Background: Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia. DM-related dyslipidemia are associated with complications resulting from progressive damage of various organs. CD36 is...Background: Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia. DM-related dyslipidemia are associated with complications resulting from progressive damage of various organs. CD36 is 88-kD, class B scavenger receptor, expressed on different types of cells. In diabetic patients, LDL particles are glycated with strong level;this increases CD36 expression, initiates foam cell formation and accelerates atherosclerosis. Objective: This study aimed to determine the correlation between serum CD36 level and lipid profile among patients with type 2 diabetes mellitus in Zeenam Specialized center, Khartoum State, Sudan, in a period between 2019 and 2022. Methodology: Hundred participants at different ages were included in this study;70 were type 2 diabetic patients (cases) and 30 apparently healthy individual (control). 3 ml of venous blood were collected from the participants by using a sterile needle and syringe into a labeled plain container. Each sample was stood until complete clot occurs. Clotted blood sample was then centrifuged to obtain the serum. Then they were used for measurement of total cholesterol, LDL cholesterol, HDL cholesterol, triglyceride and soluble CD36 levels. Total cholesterol, LDL cholesterol, HDL cholesterol and triglycerides were measured using Biosystem chemistry analyzer BTS-302. Serum CD36 was measured using Microplate Reader (URIT-660). Results: The results revealed that serum total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride levels were significantly higher in patients with type 2 diabetes mellitus compared with control (P = 0.03, P = 0.031, P = 0.000, P = 0.000) respectively, while there is no statistically significant differences in serum CD36 level between cases and control (P = 0.129). Also this study showed that there is no statistically significant correlation between serum CD36 level and total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides, age and body mass index. Conclusion: This study concluded that there is no statistically significant difference in serum CD36 level between cases and control. And sCD36 level was not correlated with total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides, body mass index, and age.展开更多
CD36是一种多肽单链的跨膜糖蛋白,属于B类清道夫受体。为更深入了解CD36基因的功能并能更好地为鸭分子育种方面提供基础参考,本研究采用相对定量PCR法检测樱桃谷鸭CD36 m RNA的组织表达谱,分析樱桃谷鸭各组织CD36 m RNA表达水平差异显...CD36是一种多肽单链的跨膜糖蛋白,属于B类清道夫受体。为更深入了解CD36基因的功能并能更好地为鸭分子育种方面提供基础参考,本研究采用相对定量PCR法检测樱桃谷鸭CD36 m RNA的组织表达谱,分析樱桃谷鸭各组织CD36 m RNA表达水平差异显著性及其与血清生化指标的相关性。结果表明:CD36 m RNA在16个组织中表达水平上存在显著差异,在皮脂、垂体、大肠和腹脂均呈高度特异表达,在下丘脑及胸肌呈中度特异表达,在心、肝、脾、肺、肾、腺胃、肌胃、小脑、大脑和小肠中呈低度特异表达;相关分析结果显示:CD36与甘油三酯呈极显著正相关(p<0.01),与总蛋白、球蛋白、低密度脂蛋白及总胆固醇呈极显著负相关(p<0.01)。由此推测鸭CD36基因可作为脂质性状选择的候选基因。展开更多
文摘Background: Diabetes mellitus (DM) is a chronic metabolic disorder characterized by hyperglycemia. DM-related dyslipidemia are associated with complications resulting from progressive damage of various organs. CD36 is 88-kD, class B scavenger receptor, expressed on different types of cells. In diabetic patients, LDL particles are glycated with strong level;this increases CD36 expression, initiates foam cell formation and accelerates atherosclerosis. Objective: This study aimed to determine the correlation between serum CD36 level and lipid profile among patients with type 2 diabetes mellitus in Zeenam Specialized center, Khartoum State, Sudan, in a period between 2019 and 2022. Methodology: Hundred participants at different ages were included in this study;70 were type 2 diabetic patients (cases) and 30 apparently healthy individual (control). 3 ml of venous blood were collected from the participants by using a sterile needle and syringe into a labeled plain container. Each sample was stood until complete clot occurs. Clotted blood sample was then centrifuged to obtain the serum. Then they were used for measurement of total cholesterol, LDL cholesterol, HDL cholesterol, triglyceride and soluble CD36 levels. Total cholesterol, LDL cholesterol, HDL cholesterol and triglycerides were measured using Biosystem chemistry analyzer BTS-302. Serum CD36 was measured using Microplate Reader (URIT-660). Results: The results revealed that serum total cholesterol, LDL cholesterol, HDL cholesterol, and triglyceride levels were significantly higher in patients with type 2 diabetes mellitus compared with control (P = 0.03, P = 0.031, P = 0.000, P = 0.000) respectively, while there is no statistically significant differences in serum CD36 level between cases and control (P = 0.129). Also this study showed that there is no statistically significant correlation between serum CD36 level and total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides, age and body mass index. Conclusion: This study concluded that there is no statistically significant difference in serum CD36 level between cases and control. And sCD36 level was not correlated with total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides, body mass index, and age.
文摘CD36是一种多肽单链的跨膜糖蛋白,属于B类清道夫受体。为更深入了解CD36基因的功能并能更好地为鸭分子育种方面提供基础参考,本研究采用相对定量PCR法检测樱桃谷鸭CD36 m RNA的组织表达谱,分析樱桃谷鸭各组织CD36 m RNA表达水平差异显著性及其与血清生化指标的相关性。结果表明:CD36 m RNA在16个组织中表达水平上存在显著差异,在皮脂、垂体、大肠和腹脂均呈高度特异表达,在下丘脑及胸肌呈中度特异表达,在心、肝、脾、肺、肾、腺胃、肌胃、小脑、大脑和小肠中呈低度特异表达;相关分析结果显示:CD36与甘油三酯呈极显著正相关(p<0.01),与总蛋白、球蛋白、低密度脂蛋白及总胆固醇呈极显著负相关(p<0.01)。由此推测鸭CD36基因可作为脂质性状选择的候选基因。
