Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate ...Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate of the two methods was beyond 70 % (P<0.01). It was concluded that the in vitro and in vivo detection methods of allergens have a high coherence and can be used as the effective ways to diagnose the allergic diseases in clinical practice.展开更多
Background A free running asthma screening test is usually used for screening exercise-induced bronchoconstriction.The total serum allergen IgE level can reveal the patient＇s atopy characteristics.Our study is to eva...Background A free running asthma screening test is usually used for screening exercise-induced bronchoconstriction.The total serum allergen IgE level can reveal the patient＇s atopy characteristics.Our study is to evaluate the diagnostic values of the combination of the two tests in asthmatic children and compare this new diagnostic method with the Intemational Study of Asthma and Allergies in Childhood （ISSAC） questionnaires and the bronchial provocation test,which are popular diagnostic tool for pediatric asthma.Methods A total of 773 school children were recruited in this study.The children＇s asthma was diagnosed by means of a combination of the free running asthma screening test and total serum allergen IgE level.The new diagnostic method value was assessed using receiver operating characteristic （ROC） curves and compared with other diagnostic tools such as ISSAC questionnaires and the bronchial provocation test.Results The AUC of this new diagnostic method was higher than 0.9.When the cut-off value of total serum allergen IgE level was ≥47 KU/L,the sensitivity and the specificity were 71.4％ and 85.1％,respectively,which were better than those of either the ISSAC questionnaires or bronchial provocation test.Conclusion The combination of the free running asthma screening test and total serum allergen IgE level may be an effective diagnostic tool for children＇s asthma.展开更多
目的调查分析大庆地区变应性鼻炎的主要变应原谱。方法采用德国Mediwiss变应原检测系统,检测大庆地区明确诊断为变应性鼻炎的患者975例,将患者分为儿童组（≤14岁）,青年组（15-35岁）,中年组（36-55岁）和老年组（≥56岁）。变应原分...目的调查分析大庆地区变应性鼻炎的主要变应原谱。方法采用德国Mediwiss变应原检测系统,检测大庆地区明确诊断为变应性鼻炎的患者975例,将患者分为儿童组（≤14岁）,青年组（15-35岁）,中年组（36-55岁）和老年组（≥56岁）。变应原分为吸入性和食入性二组,分析检测结果。结果大庆地区变应性鼻炎患者主要吸入变应原是蒿/矮豚草470/975（48.2%）,柏榆梧桐柳桦栎杨榛/白蜡/梧桐309/975（31.7%）,户尘螨231/975（23.7%）,苋/藜/葎草/蒲公英194/975（19.9%）;主要食入变应原是腰果/花生/黄豆208/975（21.3%）和贝/鱼165/975（16.9%）。儿童组的食入性变应原阳性率高于其他3组（P〈0.05）;儿童组的混合变应原阳性率高于其他3组（P〈0.05）;吸入组变应原在特异性Ig E 1-2级阳性率低于食入组变应原（P〈0.05）;吸入组变应原在3-4级阳性率高于食入变应原（P〈0.05）;吸入组变应原在5-6级阳性率高于食入变应原（P〈0.01）。老年组特异性Ig E 0级的阳性率高于其他3组（P〈0.01）;老年组5-6级的阳性率低于其他3组（P〈0.01）。结论大庆地区主要吸入变应原是蒿/矮豚草、柏榆梧桐柳桦栎杨榛/白蜡/梧桐、户尘螨和苋/藜/葎草/蒲公英,主要食入变应原是腰果/花生/黄豆和贝/鱼。儿童患者较成年患者发生食物变应的可能性大。儿童更易受多种变应原致敏发生混合过敏。吸入变应原的致敏强度高于食入变应原。老年组患者机体的免疫反应能力弱于儿童、青年和中年组患者。展开更多
目的制备用于检测血清中针对几种桃过敏原组分抗体的蛋白质芯片,为针对桃过敏原组分过敏患者的临床诊断提供快速可靠的方法。方法合成6种常见的桃过敏原组分的编码基因,分别将其插入p GAPZαA酵母表达载体,AvrⅡ单酶切线性化后,电转毕...目的制备用于检测血清中针对几种桃过敏原组分抗体的蛋白质芯片,为针对桃过敏原组分过敏患者的临床诊断提供快速可靠的方法。方法合成6种常见的桃过敏原组分的编码基因,分别将其插入p GAPZαA酵母表达载体,AvrⅡ单酶切线性化后,电转毕赤酵母SMD1168,表达和纯化相应的桃过敏原组分蛋白,并制备蛋白质芯片;用蛋白芯片检测临床血清样本中针对桃过敏原组分蛋白的抗体,与ImmunoCAP法相比,验证蛋白质芯片检测的灵敏度和特异性。结果用酵母表达系统成功表达并纯化了4种桃过敏原组分Pru p 1、Pru p 3、Pru p 4和Pru p 7,制备了能进行质量控制的检测血清桃过敏原组分抗体的蛋白质芯片。检测了疑似桃过敏患者血清41例,结果以ImmunoCAP法为金标准,蛋白芯片检测血清Pru p 1、Pru p 3和Pru p 4抗体的灵敏度分别为40%、100%和100%,特异性为78%、50%和100%;对上述3种组分抗体的综合检测灵敏度为86%,特异性为96%。针对缺乏ImmunoCAP检测结果的Pru p 7抗体,蛋白芯片检测出6份阳性的血清样本,均为桃过敏患者。结论桃过敏原组分检测芯片在灵敏度和特异性方面与ImmunoCAP相当,血清用量少,优化后有望成为临床桃过敏检测的一种新方法。展开更多
文摘Summary: In order to study the relationship between serum specific IgE (sIgE) and allergen skin test, allergen skin tests and detections of sIgE in 220 allergic patients of Wuhan area were analyzed. The coherent rate of the two methods was beyond 70 % (P<0.01). It was concluded that the in vitro and in vivo detection methods of allergens have a high coherence and can be used as the effective ways to diagnose the allergic diseases in clinical practice.
文摘Background A free running asthma screening test is usually used for screening exercise-induced bronchoconstriction.The total serum allergen IgE level can reveal the patient＇s atopy characteristics.Our study is to evaluate the diagnostic values of the combination of the two tests in asthmatic children and compare this new diagnostic method with the Intemational Study of Asthma and Allergies in Childhood （ISSAC） questionnaires and the bronchial provocation test,which are popular diagnostic tool for pediatric asthma.Methods A total of 773 school children were recruited in this study.The children＇s asthma was diagnosed by means of a combination of the free running asthma screening test and total serum allergen IgE level.The new diagnostic method value was assessed using receiver operating characteristic （ROC） curves and compared with other diagnostic tools such as ISSAC questionnaires and the bronchial provocation test.Results The AUC of this new diagnostic method was higher than 0.9.When the cut-off value of total serum allergen IgE level was ≥47 KU/L,the sensitivity and the specificity were 71.4％ and 85.1％,respectively,which were better than those of either the ISSAC questionnaires or bronchial provocation test.Conclusion The combination of the free running asthma screening test and total serum allergen IgE level may be an effective diagnostic tool for children＇s asthma.
文摘目的调查分析大庆地区变应性鼻炎的主要变应原谱。方法采用德国Mediwiss变应原检测系统,检测大庆地区明确诊断为变应性鼻炎的患者975例,将患者分为儿童组（≤14岁）,青年组（15-35岁）,中年组（36-55岁）和老年组（≥56岁）。变应原分为吸入性和食入性二组,分析检测结果。结果大庆地区变应性鼻炎患者主要吸入变应原是蒿/矮豚草470/975（48.2%）,柏榆梧桐柳桦栎杨榛/白蜡/梧桐309/975（31.7%）,户尘螨231/975（23.7%）,苋/藜/葎草/蒲公英194/975（19.9%）;主要食入变应原是腰果/花生/黄豆208/975（21.3%）和贝/鱼165/975（16.9%）。儿童组的食入性变应原阳性率高于其他3组（P〈0.05）;儿童组的混合变应原阳性率高于其他3组（P〈0.05）;吸入组变应原在特异性Ig E 1-2级阳性率低于食入组变应原（P〈0.05）;吸入组变应原在3-4级阳性率高于食入变应原（P〈0.05）;吸入组变应原在5-6级阳性率高于食入变应原（P〈0.01）。老年组特异性Ig E 0级的阳性率高于其他3组（P〈0.01）;老年组5-6级的阳性率低于其他3组（P〈0.01）。结论大庆地区主要吸入变应原是蒿/矮豚草、柏榆梧桐柳桦栎杨榛/白蜡/梧桐、户尘螨和苋/藜/葎草/蒲公英,主要食入变应原是腰果/花生/黄豆和贝/鱼。儿童患者较成年患者发生食物变应的可能性大。儿童更易受多种变应原致敏发生混合过敏。吸入变应原的致敏强度高于食入变应原。老年组患者机体的免疫反应能力弱于儿童、青年和中年组患者。
文摘目的制备用于检测血清中针对几种桃过敏原组分抗体的蛋白质芯片,为针对桃过敏原组分过敏患者的临床诊断提供快速可靠的方法。方法合成6种常见的桃过敏原组分的编码基因,分别将其插入p GAPZαA酵母表达载体,AvrⅡ单酶切线性化后,电转毕赤酵母SMD1168,表达和纯化相应的桃过敏原组分蛋白,并制备蛋白质芯片;用蛋白芯片检测临床血清样本中针对桃过敏原组分蛋白的抗体,与ImmunoCAP法相比,验证蛋白质芯片检测的灵敏度和特异性。结果用酵母表达系统成功表达并纯化了4种桃过敏原组分Pru p 1、Pru p 3、Pru p 4和Pru p 7,制备了能进行质量控制的检测血清桃过敏原组分抗体的蛋白质芯片。检测了疑似桃过敏患者血清41例,结果以ImmunoCAP法为金标准,蛋白芯片检测血清Pru p 1、Pru p 3和Pru p 4抗体的灵敏度分别为40%、100%和100%,特异性为78%、50%和100%;对上述3种组分抗体的综合检测灵敏度为86%,特异性为96%。针对缺乏ImmunoCAP检测结果的Pru p 7抗体,蛋白芯片检测出6份阳性的血清样本,均为桃过敏患者。结论桃过敏原组分检测芯片在灵敏度和特异性方面与ImmunoCAP相当,血清用量少,优化后有望成为临床桃过敏检测的一种新方法。
