Objective We aimed to investigate the cumulative effect of high CRP level and apolipoprotein B-to-apolipoprotein A-1（ApoB/ApoA-1） ratio on the incidence of ischemic stroke（IS） or coronary heart disease（CHD） in a...Objective We aimed to investigate the cumulative effect of high CRP level and apolipoprotein B-to-apolipoprotein A-1（ApoB/ApoA-1） ratio on the incidence of ischemic stroke（IS） or coronary heart disease（CHD） in a Mongolian population in China.Methods From June 2003 to July 2012,2589 Mongolian participants were followed up for IS and CHD events based on baseline investigation.All the participants were divided into four subgroups according to C-reactive protein（CRP） level and ApoB/ApoA-1 ratio.Cox proportional hazard models were used to estimate the hazard ratios（HRs） and 95% confidence intervals（CIs） for the IS and CHD events in all the subgroups.Results The HRs（95% CI） for IS and CHD were 1.33（0.84-2.12）,1.14（0.69-1.88）,and 1.91（1.17-3.11） in the ‘low CRP level with high ApoB/ApoA-1＇,‘high CRP level with low ApoB/ApoA-1＇,and ‘high CRP level with high ApoB/ApoA-1＇ subgroups,respectively,in comparison with the ‘low CRP level with low ApoB/ApoA-1＇ subgroup.The risks of IS and CHD events was highest in the ‘high CRP level with high ApoB/ApoA-1＇ subgroup,with statistical significance.Conclusion High CRP level with high ApoB/ApoA-1 ratio was associated with the highest risks of IS and CHD in the Mongolian population.This study suggests that the combination of high CRP and ApoB/ApoA-1 ratio may improve the assessment of future risk of developing IS and CHD in the general population.展开更多
BACKGROUND Previous studies have suggested that the costimulatory molecule 4-1BB plays pivotal roles in regulating immunity during chronic viral infection.However,up to now,there are few studies about 4-1BB in chronic...BACKGROUND Previous studies have suggested that the costimulatory molecule 4-1BB plays pivotal roles in regulating immunity during chronic viral infection.However,up to now,there are few studies about 4-1BB in chronic hepatitis B(CHB).AIM To clarify this issue,we report our comprehensive study results on the expression levels of 4-1BB in patients with CHB.METHODS From September 2018 to June 2019,a total of 64 patients with CHB were recruited from the Department of Hepatology,The First Hospital of Jilin University.Peripheral blood samples were collected from 52 treatment-naïve and 12 entecavir-treated patients with CHB as well as 37 healthy donors(including 24 healthy adults and 13 healthy children).The levels of soluble 4-1BB(s4-1BB)in plasma were measured by ELISA.4-1BB mRNA expression in peripheral blood mononuclear cells was detected by real-time quantitative PCR.RESULTS The s4-1BB levels in the plasma of patients with CHB were significantly higher than those in healthy adults(94.390±7.393 ng/mL vs 8.875±0.914 ng/mL,P<0.001).In addition,the s4-1BB level in plasma was significantly increased in patients with a higher viral load and a disease flare up.However,there were no significant differences between treatment-naïve and entecavir-treated patients.Interestingly,among treatment-naïve patients with CHB,the levels of s4-1BB in plasma had a significant positive correlation with hepatitis B surface antigen,hepatitis B virus DNA,hepatitis B e antigen,and triglyceride levels(r=0.748,P<0.001;r=0.406,P=0.004;r=0.356,P=0.019 and r=-0.469,P=0.007,respectively).The 4-1BB mRNA expression was higher in the peripheral blood mononuclear cells of patients with CHB than in the peripheral blood mononuclear cells of healthy adults,but the difference was not statistically significant.CONCLUSION These results suggest that the levels of s4-1BB may be associated with pathogenesis of hepatitis B virus and therefore may be a promising biomarker for disease progression.展开更多
Objective: To explore the changes of MCP-1, FKN, and related cytokines in the serum and cerebrospinal fluid (CSF) in children with epidemic encephalitis B. Methods: A total of 40 children with epidemic encephalitis B ...Objective: To explore the changes of MCP-1, FKN, and related cytokines in the serum and cerebrospinal fluid (CSF) in children with epidemic encephalitis B. Methods: A total of 40 children with epidemic encephalitis B who were admitted in our hospital from June, 2014 to June, 2017 were included in the study and divided into the severe group (n=15) and general group (n=25) according to the severity group. Moreover, 20 children who were suffered from oblique inguinal hernia, perineal adhesion, and cryptorchidism were served as the control group. The serum and CSF specimens were collected 24 h after admission and during the recovery period in children with epidemic encephalitis B. The serum specimen was collected 24 h after admission in the control group, and CSF specimen was collected during the lumbar puncture. ELISA was used to detect CMP-1, FKN, IL-1β, IL-18, and TNF-α levels in the serum and CSF. CMP-1, FKN, IL-1β, IL-18, and TNF-α levels in children with epidemic encephalitis B on the day after admission and 2-3 weeks after admission and in the control group were compared. The changes of CMP-1, FKN, IL-1β, IL-18, and TNF-α in children with severe and general epidemic encephalitis B were observed. Results: CMP-1 and FKN levels in the serum and CSF in children with epidemic encephalitis B in the critical stage were significantly higher than those in the recovery stage and in the control group. The serum CMP-1 and FKN levels in children with epidemic encephalitis B during the recovery stage were not significantly different from those in the control group, while CMP-1 and FKN levels in CSF were significantly higher than those in the control group. CMP-1 and FKN levels in the serum and CSF in children with severe epidemic encephalitis B were significantly higher than those in the general group. IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with epidemic encephalitis B during the critical stage were significantly higher than those during the recovery stage and in the control group. IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with epidemic encephalitis B during the recovery stage were significantly higher than those in the control group. IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with severe epidemic encephalitis B were significantly higher than those in the general group. Conclusions: CMP-1, FKN, IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with epidemic encephalitis B are correlated with the severity degree, detection of which can contribute to estimate the clinical typing and condition change of epidemic encephalitis.展开更多
基金supported by the National Natural Science Foundation of China(grant Nos.30972531 and 81320108026)a project of the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Objective We aimed to investigate the cumulative effect of high CRP level and apolipoprotein B-to-apolipoprotein A-1（ApoB/ApoA-1） ratio on the incidence of ischemic stroke（IS） or coronary heart disease（CHD） in a Mongolian population in China.Methods From June 2003 to July 2012,2589 Mongolian participants were followed up for IS and CHD events based on baseline investigation.All the participants were divided into four subgroups according to C-reactive protein（CRP） level and ApoB/ApoA-1 ratio.Cox proportional hazard models were used to estimate the hazard ratios（HRs） and 95% confidence intervals（CIs） for the IS and CHD events in all the subgroups.Results The HRs（95% CI） for IS and CHD were 1.33（0.84-2.12）,1.14（0.69-1.88）,and 1.91（1.17-3.11） in the ‘low CRP level with high ApoB/ApoA-1＇,‘high CRP level with low ApoB/ApoA-1＇,and ‘high CRP level with high ApoB/ApoA-1＇ subgroups,respectively,in comparison with the ‘low CRP level with low ApoB/ApoA-1＇ subgroup.The risks of IS and CHD events was highest in the ‘high CRP level with high ApoB/ApoA-1＇ subgroup,with statistical significance.Conclusion High CRP level with high ApoB/ApoA-1 ratio was associated with the highest risks of IS and CHD in the Mongolian population.This study suggests that the combination of high CRP and ApoB/ApoA-1 ratio may improve the assessment of future risk of developing IS and CHD in the general population.
基金Chinese Foundation for Hepatitis Prevention and Control—Tian-Qing Liver Disease Research Fund Subject,No.TQGB20200118.
文摘BACKGROUND Previous studies have suggested that the costimulatory molecule 4-1BB plays pivotal roles in regulating immunity during chronic viral infection.However,up to now,there are few studies about 4-1BB in chronic hepatitis B(CHB).AIM To clarify this issue,we report our comprehensive study results on the expression levels of 4-1BB in patients with CHB.METHODS From September 2018 to June 2019,a total of 64 patients with CHB were recruited from the Department of Hepatology,The First Hospital of Jilin University.Peripheral blood samples were collected from 52 treatment-naïve and 12 entecavir-treated patients with CHB as well as 37 healthy donors(including 24 healthy adults and 13 healthy children).The levels of soluble 4-1BB(s4-1BB)in plasma were measured by ELISA.4-1BB mRNA expression in peripheral blood mononuclear cells was detected by real-time quantitative PCR.RESULTS The s4-1BB levels in the plasma of patients with CHB were significantly higher than those in healthy adults(94.390±7.393 ng/mL vs 8.875±0.914 ng/mL,P<0.001).In addition,the s4-1BB level in plasma was significantly increased in patients with a higher viral load and a disease flare up.However,there were no significant differences between treatment-naïve and entecavir-treated patients.Interestingly,among treatment-naïve patients with CHB,the levels of s4-1BB in plasma had a significant positive correlation with hepatitis B surface antigen,hepatitis B virus DNA,hepatitis B e antigen,and triglyceride levels(r=0.748,P<0.001;r=0.406,P=0.004;r=0.356,P=0.019 and r=-0.469,P=0.007,respectively).The 4-1BB mRNA expression was higher in the peripheral blood mononuclear cells of patients with CHB than in the peripheral blood mononuclear cells of healthy adults,but the difference was not statistically significant.CONCLUSION These results suggest that the levels of s4-1BB may be associated with pathogenesis of hepatitis B virus and therefore may be a promising biomarker for disease progression.
文摘Objective: To explore the changes of MCP-1, FKN, and related cytokines in the serum and cerebrospinal fluid (CSF) in children with epidemic encephalitis B. Methods: A total of 40 children with epidemic encephalitis B who were admitted in our hospital from June, 2014 to June, 2017 were included in the study and divided into the severe group (n=15) and general group (n=25) according to the severity group. Moreover, 20 children who were suffered from oblique inguinal hernia, perineal adhesion, and cryptorchidism were served as the control group. The serum and CSF specimens were collected 24 h after admission and during the recovery period in children with epidemic encephalitis B. The serum specimen was collected 24 h after admission in the control group, and CSF specimen was collected during the lumbar puncture. ELISA was used to detect CMP-1, FKN, IL-1β, IL-18, and TNF-α levels in the serum and CSF. CMP-1, FKN, IL-1β, IL-18, and TNF-α levels in children with epidemic encephalitis B on the day after admission and 2-3 weeks after admission and in the control group were compared. The changes of CMP-1, FKN, IL-1β, IL-18, and TNF-α in children with severe and general epidemic encephalitis B were observed. Results: CMP-1 and FKN levels in the serum and CSF in children with epidemic encephalitis B in the critical stage were significantly higher than those in the recovery stage and in the control group. The serum CMP-1 and FKN levels in children with epidemic encephalitis B during the recovery stage were not significantly different from those in the control group, while CMP-1 and FKN levels in CSF were significantly higher than those in the control group. CMP-1 and FKN levels in the serum and CSF in children with severe epidemic encephalitis B were significantly higher than those in the general group. IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with epidemic encephalitis B during the critical stage were significantly higher than those during the recovery stage and in the control group. IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with epidemic encephalitis B during the recovery stage were significantly higher than those in the control group. IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with severe epidemic encephalitis B were significantly higher than those in the general group. Conclusions: CMP-1, FKN, IL-1β, TNF-α, and IL-18 levels in the serum and CSF in children with epidemic encephalitis B are correlated with the severity degree, detection of which can contribute to estimate the clinical typing and condition change of epidemic encephalitis.
