A Raman spectroscopy method combined with neural network is used for the invasive and rapid detection of echinococcosis. The Raman spectroscopy measurements are performed on two groups of blood serum samples,which are...A Raman spectroscopy method combined with neural network is used for the invasive and rapid detection of echinococcosis. The Raman spectroscopy measurements are performed on two groups of blood serum samples,which are from 28 echinococcosis patients and 38 healthy persons,respectively. The normalized Raman reflection spectra show that the reflectivity of the echinococcosis blood serum is higher than that of the normal human blood serum in the wavelength ranges of 101—175 nm and 1 801—2 701 nm. Then the principal component analysis(PCA) and back propagation neural network(BPNN) model are used to obtain the diagnosis results. The diagnosis rates for healthy persons and echinococcosis persons are 93.333 3% and 90.909 1%,respectively,so the average final diagnosis rate is 92.121 2%. The results demonstrate that the Raman spectroscopy analysis of blood serum combined with PCA-BPNN has considerable potential for the non-invasive and rapid detection of echinococcosis.展开更多
A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAM...A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.I. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.I. in human serum samples.展开更多
Objective To explore a highly sensitive and highly specific method to detect the serum MG7 antigen(Ag)level for early gastric cancer diagnosis.Methods The serum MG7-Ag level was detected by enzyme-linked immunosorbent...Objective To explore a highly sensitive and highly specific method to detect the serum MG7 antigen(Ag)level for early gastric cancer diagnosis.Methods The serum MG7-Ag level was detected by enzyme-linked immunosorbent assay(ELISA)method in 116 preoperative gastric cancer patients,63 postoperative gastric cancer patients,41 patients with precancerous lesion,37 pa-展开更多
a-Ketoglutaric acid(a-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body.The mutations of isocitrate dehydrogenase-1(IDH1) and isocitrate dehydrogenase-2(IDH2), however,will lead...a-Ketoglutaric acid(a-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body.The mutations of isocitrate dehydrogenase-1(IDH1) and isocitrate dehydrogenase-2(IDH2), however,will lead to the transformation of a-KA into 2-hydroxyglutarate(2-HG), which is confirmed to closely related to actue myeloid leukemia(AML). Therefore it is of great significance to detect a-KA level changes in serum. In this paper, a fluorescent "off-on" probe CH for a-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for a-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide(CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement((I - I_0)/I_0 at 550 nm) over other biological species by optimizing the chemical recognition process of CH with a-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing a-KA and may promote fluorescence detection in clinical diagnosis.展开更多
基金supported by the National Natural Science Foundation of China(No.61308120)the Doctor Startup Project of Xinjiang University(No.BS120122)+1 种基金the Young Talents Project in Xinjiang Uygur Autonomous Region(No.2013731003)the Xinjiang Science and Technology Project(Nos.201412107 and 2014211B003)
文摘A Raman spectroscopy method combined with neural network is used for the invasive and rapid detection of echinococcosis. The Raman spectroscopy measurements are performed on two groups of blood serum samples,which are from 28 echinococcosis patients and 38 healthy persons,respectively. The normalized Raman reflection spectra show that the reflectivity of the echinococcosis blood serum is higher than that of the normal human blood serum in the wavelength ranges of 101—175 nm and 1 801—2 701 nm. Then the principal component analysis(PCA) and back propagation neural network(BPNN) model are used to obtain the diagnosis results. The diagnosis rates for healthy persons and echinococcosis persons are 93.333 3% and 90.909 1%,respectively,so the average final diagnosis rate is 92.121 2%. The results demonstrate that the Raman spectroscopy analysis of blood serum combined with PCA-BPNN has considerable potential for the non-invasive and rapid detection of echinococcosis.
基金funded by the National Key Science and Technology Projects of China(2012ZX10004219 and 2013ZX10004001)
文摘A set of universal loop-mediated isothermal amplification (LAMP) primers targeting the flo gene was designed to detect Borrelia burgdorferi sensu lato (B. burgdorferi s.I.) in human samples. The sensitivity of LAMP was 20 copies/reaction, and the assay did not detect false positives among 11 other related bacteria. A positive LAMP result was obtained for 9 of the 24 confirmed cases and for 12 of 94 suspected cases. The positive rate of LAMP was the same as that of nested PCR. The LAMP is a useful diagnostic method that can be developed for rapid detection of B. burgdorferi s.I. in human sera. Combination of the LAMP and nested PCR was more sensitive for detecting B. burgdorferi s.I. in human serum samples.
文摘Objective To explore a highly sensitive and highly specific method to detect the serum MG7 antigen(Ag)level for early gastric cancer diagnosis.Methods The serum MG7-Ag level was detected by enzyme-linked immunosorbent assay(ELISA)method in 116 preoperative gastric cancer patients,63 postoperative gastric cancer patients,41 patients with precancerous lesion,37 pa-
基金supported by the National Natural Science Foundation of China (Nos. 21576037, 21422601, 21406028, 21421005)NSFC-Liaoning United Fund (No. U1608222)
文摘a-Ketoglutaric acid(a-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body.The mutations of isocitrate dehydrogenase-1(IDH1) and isocitrate dehydrogenase-2(IDH2), however,will lead to the transformation of a-KA into 2-hydroxyglutarate(2-HG), which is confirmed to closely related to actue myeloid leukemia(AML). Therefore it is of great significance to detect a-KA level changes in serum. In this paper, a fluorescent "off-on" probe CH for a-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for a-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide(CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement((I - I_0)/I_0 at 550 nm) over other biological species by optimizing the chemical recognition process of CH with a-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing a-KA and may promote fluorescence detection in clinical diagnosis.