AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori...AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori on chocolate blood agar was used to provide spiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0 4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori . RESULTS Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non ulcer dyspepsia (NUD) (44%) and other diseases (45%) ( P <0 05). IgG antibodies against spiral and coccoid antigens were detected in 50 7% (141/278) and 49 6% (138/278) , respectively. CONCLUSION The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.展开更多
AIM: To investigate catalase (KatA) and alkyl hydroperoxide reductase (AhpC) antibodies of Helicobacter pylori as biomarkers for gastric cancer (GC). METHODS: This study included 232 cases and 264 controls. Recombinan...AIM: To investigate catalase (KatA) and alkyl hydroperoxide reductase (AhpC) antibodies of Helicobacter pylori as biomarkers for gastric cancer (GC). METHODS: This study included 232 cases and 264 controls. Recombinant KatA and AhpC proteins were constructed and the levels of antibodies were tested by indirect enzyme-linked immunosorbent assay (ELISA). Logistic regression was applied to analyze the relationships between KatA, AhpC and GC. The chi(2) trend test was used to evaluate the dose-response relationships between serum KatA and AhpC antibody levels and GC. Receiver operating characteristic (ROC) curve was used to evaluate the screening accuracy of KatA and AhpC as biomarkers. Combined analysis was used to observe screening accuracy of predictors for GC. RESULTS: In all subjects, the association between KatA and AhpC and GC risk was significant (P < 0.001) with odds ratio (OR) = 12.84 (95%CI: 7.79-21.15) and OR = 2.4 (95%CI: 1.55-3.73), respectively. KatA and AhpC antibody levels were strongly related to GC risk with a dose-dependent effect (P for trend < 0.001). The area under the ROC (AUC) for KatA was 0.806, providing a sensitivity of 66.81% and specificity of 86.36%; and the AUC for AhpC was 0.615, with a sensitivity of 75.65% and specificity of 45.49%. The AUC was 0.906 for KatA and flagella protein A (FlaA) combined analysis. CONCLUSION: Serum KatA and AhpC antibodies are associated with GC risk and KatA may serve as a biomarker for GC. KatA/FlaA combined analysis improved screening accuracy.展开更多
Objective:To detect the prevalence of Helicobacter polori(H.pylori) in a group of pediatric patients complaining of gastrointestinal disorders,and to identify the virulent strains of H. pylori and its relation to pept...Objective:To detect the prevalence of Helicobacter polori(H.pylori) in a group of pediatric patients complaining of gastrointestinal disorders,and to identify the virulent strains of H. pylori and its relation to peptic disease.Methods:The study included 60 patients(41 males and 19 females),aged from 6 months to 14 years,with complaining of different gastrointestinal troubles.Anti-H.pylori IgG antibodies were used.Thorough examination of the oesophagus, stomach and duodenum was done in all patients using a fibreoptic endoscopy.In addition, gastric antral biopsies were taken for diagnosis of H.pylori infection in the following test: rapid urease test,histopathologic examination and culture.And cytotoxin assay of virulent strains was employed.H.pylori infection was diagnosed if 3 or more tests for diagnosis were found to be positive.Results:33 out of 60 patients(55%) was positive.12(20%) patients had positive toxin producing strains(virulent strains).Virulent strain had a significantly closer relationship with recurrent abdominal pain,while no difference was found in other symptoms. Age of patients showed a highly significant positive correlation with virulent H.pylori strain. Histopathological finding revealed that oesophagitis was significandy common in patients with negative H.pylori.Endoscopy and histopathological findings revealed that virulent strain had a significandy higher gastritis and oesophagitis rates.Conclusion:Infection by virulent strains of H.pylori in children is not uncommon:It is associated with endoscopic and histopathologic changes.All cases infected with virulent strain showed positive results by using rapid urease test,culture and IgG antibodies.展开更多
文摘AIM To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS Blood samples were collected from 278 patients with gastric diseases. A 3 day old culture of H. pylori on chocolate blood agar was used to provide spiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0 4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori . RESULTS Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non ulcer dyspepsia (NUD) (44%) and other diseases (45%) ( P <0 05). IgG antibodies against spiral and coccoid antigens were detected in 50 7% (141/278) and 49 6% (138/278) , respectively. CONCLUSION The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.
基金Supported by the National Natural Science Foundation of China,No.81573219Heilongjiang Province Office of Education Foundation,No.12541288
文摘AIM: To investigate catalase (KatA) and alkyl hydroperoxide reductase (AhpC) antibodies of Helicobacter pylori as biomarkers for gastric cancer (GC). METHODS: This study included 232 cases and 264 controls. Recombinant KatA and AhpC proteins were constructed and the levels of antibodies were tested by indirect enzyme-linked immunosorbent assay (ELISA). Logistic regression was applied to analyze the relationships between KatA, AhpC and GC. The chi(2) trend test was used to evaluate the dose-response relationships between serum KatA and AhpC antibody levels and GC. Receiver operating characteristic (ROC) curve was used to evaluate the screening accuracy of KatA and AhpC as biomarkers. Combined analysis was used to observe screening accuracy of predictors for GC. RESULTS: In all subjects, the association between KatA and AhpC and GC risk was significant (P < 0.001) with odds ratio (OR) = 12.84 (95%CI: 7.79-21.15) and OR = 2.4 (95%CI: 1.55-3.73), respectively. KatA and AhpC antibody levels were strongly related to GC risk with a dose-dependent effect (P for trend < 0.001). The area under the ROC (AUC) for KatA was 0.806, providing a sensitivity of 66.81% and specificity of 86.36%; and the AUC for AhpC was 0.615, with a sensitivity of 75.65% and specificity of 45.49%. The AUC was 0.906 for KatA and flagella protein A (FlaA) combined analysis. CONCLUSION: Serum KatA and AhpC antibodies are associated with GC risk and KatA may serve as a biomarker for GC. KatA/FlaA combined analysis improved screening accuracy.
文摘Objective:To detect the prevalence of Helicobacter polori(H.pylori) in a group of pediatric patients complaining of gastrointestinal disorders,and to identify the virulent strains of H. pylori and its relation to peptic disease.Methods:The study included 60 patients(41 males and 19 females),aged from 6 months to 14 years,with complaining of different gastrointestinal troubles.Anti-H.pylori IgG antibodies were used.Thorough examination of the oesophagus, stomach and duodenum was done in all patients using a fibreoptic endoscopy.In addition, gastric antral biopsies were taken for diagnosis of H.pylori infection in the following test: rapid urease test,histopathologic examination and culture.And cytotoxin assay of virulent strains was employed.H.pylori infection was diagnosed if 3 or more tests for diagnosis were found to be positive.Results:33 out of 60 patients(55%) was positive.12(20%) patients had positive toxin producing strains(virulent strains).Virulent strain had a significantly closer relationship with recurrent abdominal pain,while no difference was found in other symptoms. Age of patients showed a highly significant positive correlation with virulent H.pylori strain. Histopathological finding revealed that oesophagitis was significandy common in patients with negative H.pylori.Endoscopy and histopathological findings revealed that virulent strain had a significandy higher gastritis and oesophagitis rates.Conclusion:Infection by virulent strains of H.pylori in children is not uncommon:It is associated with endoscopic and histopathologic changes.All cases infected with virulent strain showed positive results by using rapid urease test,culture and IgG antibodies.
