AIM: To investigate catalase(Kat A) and alkyl hydroperoxide reductase(Ahp C) antibodies of Helicobacter pylori as biomarkers for gastric cancer(GC).METHODS: This study included 232 cases and 264 controls. Recombinant ...AIM: To investigate catalase(Kat A) and alkyl hydroperoxide reductase(Ahp C) antibodies of Helicobacter pylori as biomarkers for gastric cancer(GC).METHODS: This study included 232 cases and 264 controls. Recombinant Kat A and Ahp C proteins were constructed and the levels of antibodies were tested by indirect enzyme-linked immunosorbent assay(ELISA). Logistic regression was applied to analyze the relationships between Kat A, Ahp C and GC. The χ2 trend test was used to evaluate the dose-response relationships between serum Kat A and Ahp C antibody levels and GC. Receiver operating characteristic(ROC) curve was used to evaluate the screening accuracy of Kat A and Ahp C as biomarkers. Combined analysis was used to observe screening accuracy of predictors for GC.RESULTS: In all subjects, the association between Kat A and Ahp C and GC risk was significant(P < 0.001) with odds ratio(OR) = 12.84(95%CI: 7.79-21.15)and OR = 2.4(95%CI: 1.55-3.73), respectively. Kat A and Ahp C antibody levels were strongly related to GC risk with a dose-dependent effect(P for trend < 0.001). The area under the ROC(AUC) for Kat A was 0.806, providing a sensitivity of 66.81% and specificity of 86.36%; and the AUC for Ahp C was 0.615, with a sensitivity of 75.65% and specificity of 45.49%. The AUC was 0.906 for Kat A and flagella protein A(Fla A) combined analysis.CONCLUSION: Serum Kat A and Ahp C antibodies are associated with GC risk and Kat A may serve as a biomarker for GC. Kat A/Fla A combined analysis improved screening accuracy.展开更多
Objective:To detect the prevalence of Helicobacter polori(H.pylori) in a group of pediatric patients complaining of gastrointestinal disorders,and to identify the virulent strains of H. pylori and its relation to pept...Objective:To detect the prevalence of Helicobacter polori(H.pylori) in a group of pediatric patients complaining of gastrointestinal disorders,and to identify the virulent strains of H. pylori and its relation to peptic disease.Methods:The study included 60 patients(41 males and 19 females),aged from 6 months to 14 years,with complaining of different gastrointestinal troubles.Anti-H.pylori IgG antibodies were used.Thorough examination of the oesophagus, stomach and duodenum was done in all patients using a fibreoptic endoscopy.In addition, gastric antral biopsies were taken for diagnosis of H.pylori infection in the following test: rapid urease test,histopathologic examination and culture.And cytotoxin assay of virulent strains was employed.H.pylori infection was diagnosed if 3 or more tests for diagnosis were found to be positive.Results:33 out of 60 patients(55%) was positive.12(20%) patients had positive toxin producing strains(virulent strains).Virulent strain had a significantly closer relationship with recurrent abdominal pain,while no difference was found in other symptoms. Age of patients showed a highly significant positive correlation with virulent H.pylori strain. Histopathological finding revealed that oesophagitis was significandy common in patients with negative H.pylori.Endoscopy and histopathological findings revealed that virulent strain had a significandy higher gastritis and oesophagitis rates.Conclusion:Infection by virulent strains of H.pylori in children is not uncommon:It is associated with endoscopic and histopathologic changes.All cases infected with virulent strain showed positive results by using rapid urease test,culture and IgG antibodies.展开更多
基金Supported by the National Natural Science Foundation of China,No.81573219Heilongjiang Province Office of Education Foundation,No.12541288
文摘AIM: To investigate catalase(Kat A) and alkyl hydroperoxide reductase(Ahp C) antibodies of Helicobacter pylori as biomarkers for gastric cancer(GC).METHODS: This study included 232 cases and 264 controls. Recombinant Kat A and Ahp C proteins were constructed and the levels of antibodies were tested by indirect enzyme-linked immunosorbent assay(ELISA). Logistic regression was applied to analyze the relationships between Kat A, Ahp C and GC. The χ2 trend test was used to evaluate the dose-response relationships between serum Kat A and Ahp C antibody levels and GC. Receiver operating characteristic(ROC) curve was used to evaluate the screening accuracy of Kat A and Ahp C as biomarkers. Combined analysis was used to observe screening accuracy of predictors for GC.RESULTS: In all subjects, the association between Kat A and Ahp C and GC risk was significant(P < 0.001) with odds ratio(OR) = 12.84(95%CI: 7.79-21.15)and OR = 2.4(95%CI: 1.55-3.73), respectively. Kat A and Ahp C antibody levels were strongly related to GC risk with a dose-dependent effect(P for trend < 0.001). The area under the ROC(AUC) for Kat A was 0.806, providing a sensitivity of 66.81% and specificity of 86.36%; and the AUC for Ahp C was 0.615, with a sensitivity of 75.65% and specificity of 45.49%. The AUC was 0.906 for Kat A and flagella protein A(Fla A) combined analysis.CONCLUSION: Serum Kat A and Ahp C antibodies are associated with GC risk and Kat A may serve as a biomarker for GC. Kat A/Fla A combined analysis improved screening accuracy.
文摘Objective:To detect the prevalence of Helicobacter polori(H.pylori) in a group of pediatric patients complaining of gastrointestinal disorders,and to identify the virulent strains of H. pylori and its relation to peptic disease.Methods:The study included 60 patients(41 males and 19 females),aged from 6 months to 14 years,with complaining of different gastrointestinal troubles.Anti-H.pylori IgG antibodies were used.Thorough examination of the oesophagus, stomach and duodenum was done in all patients using a fibreoptic endoscopy.In addition, gastric antral biopsies were taken for diagnosis of H.pylori infection in the following test: rapid urease test,histopathologic examination and culture.And cytotoxin assay of virulent strains was employed.H.pylori infection was diagnosed if 3 or more tests for diagnosis were found to be positive.Results:33 out of 60 patients(55%) was positive.12(20%) patients had positive toxin producing strains(virulent strains).Virulent strain had a significantly closer relationship with recurrent abdominal pain,while no difference was found in other symptoms. Age of patients showed a highly significant positive correlation with virulent H.pylori strain. Histopathological finding revealed that oesophagitis was significandy common in patients with negative H.pylori.Endoscopy and histopathological findings revealed that virulent strain had a significandy higher gastritis and oesophagitis rates.Conclusion:Infection by virulent strains of H.pylori in children is not uncommon:It is associated with endoscopic and histopathologic changes.All cases infected with virulent strain showed positive results by using rapid urease test,culture and IgG antibodies.
