Objectives To investigate the relationship of serum leptin concentration with the lipids and body mass index （BMI） in patients with chronic congestive heart failure（CHF）. Methods The serum concentration of leptin ...Objectives To investigate the relationship of serum leptin concentration with the lipids and body mass index （BMI） in patients with chronic congestive heart failure（CHF）. Methods The serum concentration of leptin in 39 patients with CHF （14 in cardiac function class Ⅱ , 21 in class Ⅲ, 4 in classⅣ , NYHA） and in 46 patients with cardiac function class Ⅰ （NYHA） were assessed by radioimmunoassay. Results The serum concentration of leptin were 9.018±4.519 μg/l in CHF group （cardiac function class Ⅱ 11.492±5.649 μg/l, class Ⅲ 7.763±3.321 μg/l, class Ⅳ 6.100±2.657 μg/l）; 11.674± 6.911 μg/l in class Ⅰ group. The serum concentrations of leptin were significantly lower in CHF group, as compared with class Ⅰ group （P〈 0.05）. Moreover, the decrease of serum leptin concentration was significantly correlated with the decreased serum concentrations of total cholesterol, triglyceride, body mass index and left ventricular ejection fraction in CHF group, respectively （P 〈 0.05）. Conclusions The significance of the decrease in serum leptin in CHF patients needs further study.展开更多
Objective To explore the difference of low-frequency electroacupuncture in regulating serum leptin and neuropeptide Y（NPY） of male and female patients with simple obesity due to spleen deficiency and damp exuberance...Objective To explore the difference of low-frequency electroacupuncture in regulating serum leptin and neuropeptide Y（NPY） of male and female patients with simple obesity due to spleen deficiency and damp exuberance. Methods Eighty patients with simple obesity, including 37 males and 43 females, were included in this study. In both groups（male group and female group）, Yīnlíngquán（阴陵泉 SP 9）, Sānyīnjiāo（三阴交 SP 6）, Zúsānlǐ（足三里 ST 36）, Fēnglóng（丰隆 ST 40）, Qūchí（曲池 LI 11）, Tiānshū（天枢 ST 25）, Zhōngwǎn（中脘 CV 12）, Shuǐfēn（水分 CV 9）, Qìhǎi（气海 CV 6）, and Guānyuán（关元 CV 4）. were selected, and low-frequency electroacupuncture treatment was applied. Needles were retained for 30 min. The treatment was conducted once a day, and 10 times were considered as one course of treatment. Two courses were needed. The changes of weight, BMI（body mass index）, F%（fat percentage） and serum leptin and NPY contents of patients in the two groups before and after treatment were observed and analyzed comparatively. Results After treatment, in male group, the weight lost（9.24±2.55） kg, BMI reduced by 4.19±2.30, F% reduced by 3.98±2.13, serum leptin level reduced by（0.66±0.23） ng/m L, and serum NPY level reduced by（18.56±2.89） pg/m L; in female group, the weight lost（6.77±2.31） kg, BMI reduced by 2.65±0.93, F% reduced by 4.98±2.30, serum leptin level reduced by（3.49±1.09） ng/m L, and serum NPY level reduced by（11.21±1.97） pg/m L. The results reduced significantly when compared with the level before treatment（P〈0.05, P〈0.01）. The loss of weight, BMI and serum NPY content in male group was more obvious than that in female group（all P〈0.01）, and the loss of F% and serum leptin content in female group was more obvious than that in male group（both P〈0.01）. Conclusion Low-frequency electroacupuncture can significantly regulate the weight, BMI, F% and serum leptin and NPY contents of male and female patients with simple obesity, and there are gender differences. In terms of reducing weight, BMI and serum NPY content, the efficacy of low-frequency electroacupuncture in males is superior to that in females, while in terms of reducing F% and serum leptin content, the efficacy in females is superior to that in males.展开更多
Despite the extensive clinical experience with the use of metformin worldwide, no formal dose-ranging study has been conducted because the current dosing strategy of metformin was determined empirically, rather than b...Despite the extensive clinical experience with the use of metformin worldwide, no formal dose-ranging study has been conducted because the current dosing strategy of metformin was determined empirically, rather than by an understanding of its dose-response relationship in patients with type 2 diabetes. The present study was designed to evaluate the correlation between serum metformin levels and glycemic control, insulin resistance and leptin levels in females newly diagnosed with type 2 diabetes. Sixty type 2 diabetic females were recruited for the study and were allocated into 3 groups;each receiving metformin 1000, 1500 and 2000 mg/day respectively for 3 months. Blood samples withdrawn from each patient at zero time and after 3 months is used to evaluate serum levels of HbA1c, glucose, leptin and insulin, in addition, the measurement of serum level of metformin in blood after 3 months by HPLC. The results demonstrated that all the treated groups with different doses of metformin showed significant improve in all parameters;the use of increased metformin doses was only correlated with plasma leptin levels in the highest dose. In conclusion, serum metformin levels are not good predictors for correlating improvement in clinical and biochemical parameters with increasing the dose in newly diagnosed non-insulin resistant females with type 2 diabetes.展开更多
目的:研究脓毒败血症对外周血瘦素(Leptin)表达水平的影响,探讨Leptin在急性创伤炎症反应中的作用。方法:建立大鼠盲肠结扎穿孔模型,设立假手术组、单纯损伤组和脂肪乳、雌二醇、胰岛素组等干预能量代谢和神经-内分泌功能的实验组,每组1...目的:研究脓毒败血症对外周血瘦素(Leptin)表达水平的影响,探讨Leptin在急性创伤炎症反应中的作用。方法:建立大鼠盲肠结扎穿孔模型,设立假手术组、单纯损伤组和脂肪乳、雌二醇、胰岛素组等干预能量代谢和神经-内分泌功能的实验组,每组10只,并采用自建的高灵敏大鼠Leptin R IA测量损伤后12h血清Leptin浓度的变化。结果:所用Leptin R IA标准曲线形态良好,可获得各实验组血清Leptin浓度。与伤后假手术组血清Leptin水平相比,其他四组与之无显著差异,脂肪乳组有升高的趋势,而其他三组均为降低的趋势。另外,脂肪乳组显著高于单纯损伤组和雌二醇组。结论:脓毒败血症引起内源性Leptin表达不足,早期给予能量补充有望升高Leptin表达水平,而干扰下丘脑-垂体轴功能或降低能量储备则会抑制Leptin所发挥的保护效应。展开更多
Objective: To observe the effect of acupuncture on hyperleptinaemia and hyperinsulinemia for studying its underlying mechanism about anti-obesity and reducing blood lipid in obesity rats. Methods: A total of 80 SD rat...Objective: To observe the effect of acupuncture on hyperleptinaemia and hyperinsulinemia for studying its underlying mechanism about anti-obesity and reducing blood lipid in obesity rats. Methods: A total of 80 SD rats were randomized into normal control, model, acupuncture and medication groups, with 20 cases in each group. Hypothalamic obesity model was established by subcutaneous injection of 15% sodium glutamate (0.2 mL/10 g body weight), once daily and continuously for 5 days. “Zusanli” (足三里 ST 36), “Sanyinjiao”(三阴交 SP 6), “Guanyuan”(关元 CV 4) and “Zhongwan”(中脘 CV 12) were punctured and stimulated electrically (100 Hz, dense-sparse waves, and a suitable strength inducing local muscular tremor) for 15 min, once daily. In medication group, rats were fed with Sibutramine 4 mg/kg, once daily. After 4 weeks’ treatment, Lee’s index was detected, and serum leptin and insulin contents were determined by radioimmunoassay (RIA). Results: Compared with normal control group, Lee’s index, serum leptin and insulin contents of model group increased significantly (P<0.01). Comparison between acupuncture and model groups, Lee’s index and serum leptin of acupuncture group decreased significantly (P<0.01), and serum insulin level also lowered. In comparison with model group, Lee’s index, serum leptin and insulin levels of medication group also lowered. Comparison between acupuncture and medication groups showed that Lee’s index and serum leptin level of acupuncture group were significantly lower than those of the later group (P<0.01), displaying that the therapeutic effect of acupuncture is better than that of Sibutramine in lowering Lee’s index and serum leptin. No significant differences were found between acupuncture and medication groups in body weight and length and serum insulin(P>0.05). Conclusion: Acupuncture can effectively reduce Lee’s index, serum leptin and insulin contents in fasting obese rats, which may contribute to its effect in anti-obesity.展开更多
目的:探究并分析白芍总苷治疗对幼年特发性关节炎患儿血清Leptin、IP-10及hs-CRP表达的影响及意义。方法:选取上海六院及本院收治的特发性关节炎(JIA)患儿80例,随机将其分为观察组和对照组,每组40例。对照组给予常规治疗,观察组在对照...目的:探究并分析白芍总苷治疗对幼年特发性关节炎患儿血清Leptin、IP-10及hs-CRP表达的影响及意义。方法:选取上海六院及本院收治的特发性关节炎(JIA)患儿80例,随机将其分为观察组和对照组,每组40例。对照组给予常规治疗,观察组在对照组基础上给予足量白芍总苷进行治疗,测定两组治疗前后各个时间血清瘦素(Leptin)、大鼠干扰素诱导蛋白-10(IP-10)及超敏c反应蛋白(hsCRP)表达水平,观察并统计测定结果。结果:两组治疗后6m Leptin含量明显低于治疗前,观察组治疗后6mLeptin水平明显低于对照组,差异均具有统计学意义(P<0.05);两组治疗后6mhs-CRP水平明显低于治疗前,观察组治疗后6 m Hs-CRP水平明显低于对照组,差异均具有统计学意义(P<0.05);两组治疗后6个月IP-10水平较治疗前明显降低,但观察组明显低于对照组,差异均具有统计学意义(P<0.05)。结论:白芍总苷能有效降低幼年特发性关节炎患儿血清Leptin、IP-10及hs-CRP表达水平,有助于机体抗炎功能的充分发挥,促进健康恢复。展开更多
目的:探讨2型糖尿病合并不同分级高血压患者血清瘦素和α-黑色素细胞刺激素(α-MSH)水平的变化。方法:选取我院197例2型糖尿病患者作为研究对象,依据2010年中国高血压防治指南推荐的测量要求及高血压诊断和分级标准将197例患者进行分组...目的:探讨2型糖尿病合并不同分级高血压患者血清瘦素和α-黑色素细胞刺激素(α-MSH)水平的变化。方法:选取我院197例2型糖尿病患者作为研究对象,依据2010年中国高血压防治指南推荐的测量要求及高血压诊断和分级标准将197例患者进行分组:单纯2型糖尿病组[DM组,收缩压<140 mm Hg(1 mm Hg=0.133 k Pa)和舒张压<90 mm Hg] 45例;2型糖尿病合并1级高血压组[DH1组,收缩压140~159 mm Hg和(或)舒张压90~99 mm Hg]47例;2型糖尿病合并2级高血压组[DH2组,收缩压160~179 mm Hg和(或)舒张压100~109 mm Hg]51例;2型糖尿病合并3级高血压组[DH3组,收缩压≥180 mm Hg和(或)舒张压≥110 mm Hg]54例。另选取40名健康体检者作为正常对照组。应用酶联免疫吸附法测定血清瘦素和α-MSH水平。应用多元逐步回归法分析二者与各影响因素的相关性。结果:(1)血清瘦素水平:与正常对照组比较,其他各组明显升高,且随血压分级的增加,DH1组、DH2组和DH3组血清瘦素水平逐渐升高,以DH3组最高(P均<0.05);(2)血清α-MSH水平:与正常对照组比较,其他各组明显降低,且DH2组和DH3组血清α-MSH水平均明显低于DM组,以DH3组最低(P均<0.05);(3)相关分析结果显示:收缩压和稳态模型评估-胰岛素抵抗指数(HOMA-IR)与血清瘦素呈正相关(r=0.58,P=0.00; r=0.59,P=0.00),而与血清α-MSH呈负相关(r=-0.61,P=0.00; r=-0.59,P=0.00);进一步多元回归分析提示,HOMA-IR和收缩压均是血清瘦素和α-MSH的独立影响因素。结论:2型糖尿病合并不同分级高血压时,随着高血压分级升高,血清瘦素水平逐渐升高,血清α-MSH水平逐渐下降,表明二者可能共同对2型糖尿病合并高血压的发生有一定的影响。展开更多
文摘Objectives To investigate the relationship of serum leptin concentration with the lipids and body mass index （BMI） in patients with chronic congestive heart failure（CHF）. Methods The serum concentration of leptin in 39 patients with CHF （14 in cardiac function class Ⅱ , 21 in class Ⅲ, 4 in classⅣ , NYHA） and in 46 patients with cardiac function class Ⅰ （NYHA） were assessed by radioimmunoassay. Results The serum concentration of leptin were 9.018±4.519 μg/l in CHF group （cardiac function class Ⅱ 11.492±5.649 μg/l, class Ⅲ 7.763±3.321 μg/l, class Ⅳ 6.100±2.657 μg/l）; 11.674± 6.911 μg/l in class Ⅰ group. The serum concentrations of leptin were significantly lower in CHF group, as compared with class Ⅰ group （P〈 0.05）. Moreover, the decrease of serum leptin concentration was significantly correlated with the decreased serum concentrations of total cholesterol, triglyceride, body mass index and left ventricular ejection fraction in CHF group, respectively （P 〈 0.05）. Conclusions The significance of the decrease in serum leptin in CHF patients needs further study.
基金Supported by Science-technology support project of Hebei Provincial Administration of Traditional Chinese Medicine:2014015Youth fund project of Hebei University of Chinese Medicine:QNZ2014023
文摘Objective To explore the difference of low-frequency electroacupuncture in regulating serum leptin and neuropeptide Y（NPY） of male and female patients with simple obesity due to spleen deficiency and damp exuberance. Methods Eighty patients with simple obesity, including 37 males and 43 females, were included in this study. In both groups（male group and female group）, Yīnlíngquán（阴陵泉 SP 9）, Sānyīnjiāo（三阴交 SP 6）, Zúsānlǐ（足三里 ST 36）, Fēnglóng（丰隆 ST 40）, Qūchí（曲池 LI 11）, Tiānshū（天枢 ST 25）, Zhōngwǎn（中脘 CV 12）, Shuǐfēn（水分 CV 9）, Qìhǎi（气海 CV 6）, and Guānyuán（关元 CV 4）. were selected, and low-frequency electroacupuncture treatment was applied. Needles were retained for 30 min. The treatment was conducted once a day, and 10 times were considered as one course of treatment. Two courses were needed. The changes of weight, BMI（body mass index）, F%（fat percentage） and serum leptin and NPY contents of patients in the two groups before and after treatment were observed and analyzed comparatively. Results After treatment, in male group, the weight lost（9.24±2.55） kg, BMI reduced by 4.19±2.30, F% reduced by 3.98±2.13, serum leptin level reduced by（0.66±0.23） ng/m L, and serum NPY level reduced by（18.56±2.89） pg/m L; in female group, the weight lost（6.77±2.31） kg, BMI reduced by 2.65±0.93, F% reduced by 4.98±2.30, serum leptin level reduced by（3.49±1.09） ng/m L, and serum NPY level reduced by（11.21±1.97） pg/m L. The results reduced significantly when compared with the level before treatment（P〈0.05, P〈0.01）. The loss of weight, BMI and serum NPY content in male group was more obvious than that in female group（all P〈0.01）, and the loss of F% and serum leptin content in female group was more obvious than that in male group（both P〈0.01）. Conclusion Low-frequency electroacupuncture can significantly regulate the weight, BMI, F% and serum leptin and NPY contents of male and female patients with simple obesity, and there are gender differences. In terms of reducing weight, BMI and serum NPY content, the efficacy of low-frequency electroacupuncture in males is superior to that in females, while in terms of reducing F% and serum leptin content, the efficacy in females is superior to that in males.
文摘Despite the extensive clinical experience with the use of metformin worldwide, no formal dose-ranging study has been conducted because the current dosing strategy of metformin was determined empirically, rather than by an understanding of its dose-response relationship in patients with type 2 diabetes. The present study was designed to evaluate the correlation between serum metformin levels and glycemic control, insulin resistance and leptin levels in females newly diagnosed with type 2 diabetes. Sixty type 2 diabetic females were recruited for the study and were allocated into 3 groups;each receiving metformin 1000, 1500 and 2000 mg/day respectively for 3 months. Blood samples withdrawn from each patient at zero time and after 3 months is used to evaluate serum levels of HbA1c, glucose, leptin and insulin, in addition, the measurement of serum level of metformin in blood after 3 months by HPLC. The results demonstrated that all the treated groups with different doses of metformin showed significant improve in all parameters;the use of increased metformin doses was only correlated with plasma leptin levels in the highest dose. In conclusion, serum metformin levels are not good predictors for correlating improvement in clinical and biochemical parameters with increasing the dose in newly diagnosed non-insulin resistant females with type 2 diabetes.
文摘目的:研究脓毒败血症对外周血瘦素(Leptin)表达水平的影响,探讨Leptin在急性创伤炎症反应中的作用。方法:建立大鼠盲肠结扎穿孔模型,设立假手术组、单纯损伤组和脂肪乳、雌二醇、胰岛素组等干预能量代谢和神经-内分泌功能的实验组,每组10只,并采用自建的高灵敏大鼠Leptin R IA测量损伤后12h血清Leptin浓度的变化。结果:所用Leptin R IA标准曲线形态良好,可获得各实验组血清Leptin浓度。与伤后假手术组血清Leptin水平相比,其他四组与之无显著差异,脂肪乳组有升高的趋势,而其他三组均为降低的趋势。另外,脂肪乳组显著高于单纯损伤组和雌二醇组。结论:脓毒败血症引起内源性Leptin表达不足,早期给予能量补充有望升高Leptin表达水平,而干扰下丘脑-垂体轴功能或降低能量储备则会抑制Leptin所发挥的保护效应。
文摘Objective: To observe the effect of acupuncture on hyperleptinaemia and hyperinsulinemia for studying its underlying mechanism about anti-obesity and reducing blood lipid in obesity rats. Methods: A total of 80 SD rats were randomized into normal control, model, acupuncture and medication groups, with 20 cases in each group. Hypothalamic obesity model was established by subcutaneous injection of 15% sodium glutamate (0.2 mL/10 g body weight), once daily and continuously for 5 days. “Zusanli” (足三里 ST 36), “Sanyinjiao”(三阴交 SP 6), “Guanyuan”(关元 CV 4) and “Zhongwan”(中脘 CV 12) were punctured and stimulated electrically (100 Hz, dense-sparse waves, and a suitable strength inducing local muscular tremor) for 15 min, once daily. In medication group, rats were fed with Sibutramine 4 mg/kg, once daily. After 4 weeks’ treatment, Lee’s index was detected, and serum leptin and insulin contents were determined by radioimmunoassay (RIA). Results: Compared with normal control group, Lee’s index, serum leptin and insulin contents of model group increased significantly (P<0.01). Comparison between acupuncture and model groups, Lee’s index and serum leptin of acupuncture group decreased significantly (P<0.01), and serum insulin level also lowered. In comparison with model group, Lee’s index, serum leptin and insulin levels of medication group also lowered. Comparison between acupuncture and medication groups showed that Lee’s index and serum leptin level of acupuncture group were significantly lower than those of the later group (P<0.01), displaying that the therapeutic effect of acupuncture is better than that of Sibutramine in lowering Lee’s index and serum leptin. No significant differences were found between acupuncture and medication groups in body weight and length and serum insulin(P>0.05). Conclusion: Acupuncture can effectively reduce Lee’s index, serum leptin and insulin contents in fasting obese rats, which may contribute to its effect in anti-obesity.
文摘目的:探究并分析白芍总苷治疗对幼年特发性关节炎患儿血清Leptin、IP-10及hs-CRP表达的影响及意义。方法:选取上海六院及本院收治的特发性关节炎(JIA)患儿80例,随机将其分为观察组和对照组,每组40例。对照组给予常规治疗,观察组在对照组基础上给予足量白芍总苷进行治疗,测定两组治疗前后各个时间血清瘦素(Leptin)、大鼠干扰素诱导蛋白-10(IP-10)及超敏c反应蛋白(hsCRP)表达水平,观察并统计测定结果。结果:两组治疗后6m Leptin含量明显低于治疗前,观察组治疗后6mLeptin水平明显低于对照组,差异均具有统计学意义(P<0.05);两组治疗后6mhs-CRP水平明显低于治疗前,观察组治疗后6 m Hs-CRP水平明显低于对照组,差异均具有统计学意义(P<0.05);两组治疗后6个月IP-10水平较治疗前明显降低,但观察组明显低于对照组,差异均具有统计学意义(P<0.05)。结论:白芍总苷能有效降低幼年特发性关节炎患儿血清Leptin、IP-10及hs-CRP表达水平,有助于机体抗炎功能的充分发挥,促进健康恢复。
文摘目的:探讨2型糖尿病合并不同分级高血压患者血清瘦素和α-黑色素细胞刺激素(α-MSH)水平的变化。方法:选取我院197例2型糖尿病患者作为研究对象,依据2010年中国高血压防治指南推荐的测量要求及高血压诊断和分级标准将197例患者进行分组:单纯2型糖尿病组[DM组,收缩压<140 mm Hg(1 mm Hg=0.133 k Pa)和舒张压<90 mm Hg] 45例;2型糖尿病合并1级高血压组[DH1组,收缩压140~159 mm Hg和(或)舒张压90~99 mm Hg]47例;2型糖尿病合并2级高血压组[DH2组,收缩压160~179 mm Hg和(或)舒张压100~109 mm Hg]51例;2型糖尿病合并3级高血压组[DH3组,收缩压≥180 mm Hg和(或)舒张压≥110 mm Hg]54例。另选取40名健康体检者作为正常对照组。应用酶联免疫吸附法测定血清瘦素和α-MSH水平。应用多元逐步回归法分析二者与各影响因素的相关性。结果:(1)血清瘦素水平:与正常对照组比较,其他各组明显升高,且随血压分级的增加,DH1组、DH2组和DH3组血清瘦素水平逐渐升高,以DH3组最高(P均<0.05);(2)血清α-MSH水平:与正常对照组比较,其他各组明显降低,且DH2组和DH3组血清α-MSH水平均明显低于DM组,以DH3组最低(P均<0.05);(3)相关分析结果显示:收缩压和稳态模型评估-胰岛素抵抗指数(HOMA-IR)与血清瘦素呈正相关(r=0.58,P=0.00; r=0.59,P=0.00),而与血清α-MSH呈负相关(r=-0.61,P=0.00; r=-0.59,P=0.00);进一步多元回归分析提示,HOMA-IR和收缩压均是血清瘦素和α-MSH的独立影响因素。结论:2型糖尿病合并不同分级高血压时,随着高血压分级升高,血清瘦素水平逐渐升高,血清α-MSH水平逐渐下降,表明二者可能共同对2型糖尿病合并高血压的发生有一定的影响。
