The proprotein convertase subtilisin/kexin type 9 geneE670G polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup of the Yao minority in China.The present study was undertaken association of PCSK9 E670G polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 649 subjects of Bai Ku Yao and 646 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PCSK9 E670G polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) and apolipoprotein(Apo) AI were lower in Bai Ku Yao than in Han(P【0.01 for all).The frequency of A and G alleles was 98.00%and 2.00%in Bai Ku Yao,and 95.20%and 4.80%in Han(P【0.01);respectively. The frequency of AA,AG and GG genotypes was 95.99%,4.01%and 0%in Bai Ku Yao,and 91.02%, 8.36%and 0.62%in Han(P【0.01);respectively.There were also significant differences in the genotypic and allelic frequencies between n and the ratio of ApoAI to ApoB in Han Chinese but not in Bai Ku Yao were different between the AA and AG/GG genotypes(P【0.05 for all).The G allele carriers had higher serum HDL-C and higher ApoAI to ApoB ratio than the G allele noncarriers.When serum lipid parameters in Han were analyzed according to sex,the G allele carriers had higher serum HDL and ApoAI levels in males (P【0.05),and lower ApoB level and higher ApoAI to ApoB ratio in females(P【0.05 for all).Multiple linear regression analysis showed that serum HDL-C levels were correlated with genotypes in both ethnic groups(P【0.05 each).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups(P【0.05-0.001).Conclusions These results suggest that the PCSK9 E670G polymorphism is mainly associated with some serum lipid parameters in the Han population,both gender show different relations to different serum lipid parameters.The G allele carriers might have higher serum lipid profiles than the G allele noncarriers. ormal LDL-C(≤3.20 mmol/L) and high LDL-C subgroups (】 3.20 mmol/L,P【0.01;respectively) in Bai Ku Yao, and between normal ApoB(≤1.14 g/L) and high ApoB subgroups(】 1.14 g/L,P 【 0.01;respectively) in Han.

Interactions of the apolipoprotein A5 gene polymorphisms and alcohol consumption on serum lipid levels

Objectives Apolipoprotein(Apo) A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG) levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15 -89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol (TC),TG,high-density lipoprotein cholesterol(HDL-C), ApoA1 and ApoB were higher in drinkers than in nondrinkers (P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs) were not different between the two groups.The levels of TG in non-drinkers, and TC,TG,low-density lipoprotein cholesterol (LDL-C)and ApoB in drinkers were different among the three -1131T】C genotypes(P【0.05-0.001).The -1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all), but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types (P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T, G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of all haplotypes in the both populations.The ApoA5 haplotypes were significantly(P【0.05) associated at the global level with TC,TG,HDL-C, LDL-C,Apo1,and ApoB,even after correction for multiple testing with permutation test.In particular,carriers of haplo-type G-G-C had significantly higher TC,TG,LDL-C,ApoB than noncarriers,whereas carriers of haplotype C-A-T had significantly lower TC,LDL-C,ApoAl and ApoB,and higher HDL-C than noncarriers.Serum TC levels in nondrinkers were correlated with -1131T】C genotype and allele(P【0.05 for each),whereas serum TC,TG and LDL-C levels in drinkers were associated with -1131 T】C and C.553G】T genotypes,or c.457G】A alleles(P【0.05-0.001).Serum lipid parameters were also correlated with several environmental factors in the both groups.Conclusions The differences in serum lipid profiles between the drinkers and nondrinkers might partly result from different interactions of ApoA5 gene polymor phisms and alcohol consumption.genotypes and -1131T】C, c.553G】T and c.457G】A to detect the interactions of the ApoA5

Association of methylenetetrahydrofolate reductase C677T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Objectives The association of methylenetetrahy-drofolate reductase(MTHFR) gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in China. The aim of the present study was to eveluate the association of MTHFR C677Tpolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C), apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 77.4%and 22.6%in Bai Ku Yao,and 60.9%and 39.1%in Han(P【0.001);respectively.The frequency of CC,CT and TT genotypes was 58.7%,37.3%and 4.0%in Bai Ku Yao,and 32.6%,56.4%and 11.0%in Han(P【 0.001);respectively.The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes(P【0.05-0.01).The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers. The levels of ApoB in Han were significant differences among the three genotypes(P【0.05).The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers. The levels of TC,TG and LDL-C in Bai Ku Yao were correlated with genotypes(P【0.05-0.001),whereas the levels of LDL-C in Han were associated with genotypes(P【 0.001).Serum lipid parameters were also correlated with sex, age,body massindex,alcohol consumption,cigarette smoking, and blood pressure in the both ethnic groups.Conclusions The differences in serum TC,TG,LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677Tor differentMTHFR gene-enviromental interactions.

Relationship between the low density lipoprotein receptor gene polymorphism and serum lipid levels in the Guangxi Bai Ku Yao population

Objectives Bai Ku Yao(White-trousers Yaos)is a special branch of Yao minority in China.They are now living in both Lihu and Baxu villages,Nandan County, Guangxi,China.The population size is about 30,000.The special customs and culture of Bai Ku Yao,including their special clothing,intra-ethnic marriages and alcohol intake are still completely conserved to the present day.In previous epidemiologic studies,we found that the serum lipid levels and the prevalence of hyperlipidaemia were lower in Bai Ku Yao than in Han Chinese from the same region.This ethnic difference in serum lipid profiles is still not well known.We hypothesized that there may be significant differences in some genetic polymorphismsssociation of low density lipoprotein receptor (LDL-R) genepolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 1024 subjects of Bai Ku Yao and 792 participants of Han Chinese were stud- ied by a stratified randomized cluster sampling.Epidemiological survey was carried out using internationally standardized methods.Information on demographics,socioeconomic status, and lifestyle factors was collected with standardized questionnaires. The height,weight,waist circumference,blood pressure, and serum total cholesterol(TC),triglyceride(TG), high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein(Apo) A1, and ApoB were measured.Body massindex(BMI,kg/m2) was calculated.Genotyping of the LDL-RAvaⅡwas performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results(l)The height,weight,serum TC,HDL-C,LDL-C,ApoAl levels and the ratio of ApoAl to ApoB were lower in Bai Ku Yao than in Han Chinese(P【0.01 for all),whereas the percentage of subjects who consumed alcohol or smoked cigarettes was higher in Bai Ku Yao than in Han Chinese(P【0.01 for each).(2) The frequency of A+ allele in Bai Ku Yao was 34.5%,and the frequencies of A-A-,A-A+ and A+A + genotypes were 42.6%,45.9%and 11.5%;respectively. The frequency of A+ allele in Han Chinese was 19.3%(P【0.001),and the frequencies of A-A-,A-A + and A+A+ genotypes were 64.9%,31.6%and 3.5%(P【0.001);respectively. The frequencies of A-A-,A-A+ and A+A+ genotypes in Bai Ku Yao were significant difference between males and females,between normal TC and high TC subgroup, and between normal LDL-C and high LDL-C subgroup (P【0.05 for all),whereas the frequencies of A- and A+ ? alleles in Han Chinese were significant difference between males and females(P【0.05).(3) Serum LDL-C levels in Bai Ku Yao were significant difference among the A-A-, A-A+ and A+A+ genotypes(P【0.05),the A+ carriers had higher serum LDL-C levels.Serum HDL-C levels in Han Chiese were significant difference among the A-A-,A-A + and A+A+ genotypes(P【0.01),the A+ carriers had higher serum HDL-C levels.(4) After adjusting other factors,the prevalence of LDL-C abnormality was still higher in Han Chiese than in Bai Ku Yao.The prevalence of TC abnormality in Han Chinese was almost twice high as in Bai Ku Yao. The age and diet were common risk factor for TC abnormality. No effect of AvaⅡgenotype or alcohol consumption on the TC abnormality was found,but the combination of geno-type and alcohol consumption can increase the prevalence of TC abnormality[Exp(B) =(1.154)].Age was negatively cor- related with TG level.Conclusions Serum TC and LDL-C levels were lower in Bai Ku Yao than in Han Chinese.There were significant differences in the AvaⅡallele and genotype frequencies between the he A+ carriers in Bai Ku Yao had higher serum LDL-C levels,whereas the A+ carriers in Han had higher serum HDL-C levels.Interactions between alcohol consumption or cigarette smoking and the LDL-R AvaⅡgenotype were also observed.The differences in the serum lipid profiles between the two ethnic groups might partly result from different genotypic frequency of LDL-R AvaⅡpolymorphism or differentgene-enviromental interactions.Bai Ku Yao and Han population,the frequency of A + allele was higher in Bai Ku Yao than in Han.T between the two ethnic groups.Therefore,the aim of the present study was to detect the

Peroxisome proliferator-activated receptor delta+294T>C polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Objectives The association of peroxisome prolif-erator -activated receptor delta(PPARD) +294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of PPARD +294T】C(rs2016520) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 609 subjects of Bai Ku Yao and 573 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PPARD +294T】C polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),apolipoprotein(Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.001 for all).The frequency of T and C alleles was 77.50%and 22.50%in Bai Ku Yao,and 72.43%and 27.57%in Han (P【0.01);respectively.The frequency of TT,TC and CC genotypes was 60.59%,33.83%and 5.53%in Bai Ku Yao, and 52.18%,40.50%and 7.32%in Han(P【0.05);respectively. The levels of LDL-C,ApoB and the ratio of ApoAI to ApoB in Bai Ku Yao were different among the three genotypes in females but not in males(P【0.05 for all).The subjects with TT and TC genotypes had lower serum LDL-C and ApoB levels and higher the ratio of ApoAI to ApoB than the CC genotype in females.The levels of TC and ApoB in the total Han population were different among the three genotypes (P【0.05 for all).The C allele carriers had higher serum TC and ApoB levels than the C allele noncarriers.When serum lipid levels were analyzed according to sex,the difference in serum TC levels in Han was significant in males(P【0.01) but not in females,whereas the difference in serum ApoB levels was significant in females(P【0.05)but not in males. Serum TC and ApoB levels were correlated with genotypes in Han(P【0.05 for each) but not in Bai Ku Yao.Serum lipid parameters were also correlated with sex,age,body massindex, alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups.Conclusions These results suggest that the association of PPARD +294T】C polymorphism and serum lipid levels is different between the Bai Ku Yao and Han populations.The discrepancy between the two ethnic groups might partly result from different PPARD +294T】C polymorphism or PPARD gene-enviromental interactions,subgroup of the Yao minority in China.

Association of the LIPG 584C>T polymorphism and serum lipid levels in the Guangxi Bai Ku Yao and Han populations

Objectives Endotheliallipase(EL) is a major determinantof high-density lipoprotein-cholesterol(HDL-C) metabolism,but the association of endothelial lipase gene (LIPG) polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations.Bai Ku Yao is an isolated subgroup of the Yao minority in China.This study was designed to detect the association of LIPG 584C】T(rs2000813) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods A total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the LIPG584C】T was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),HDL-C, low-density lipoprotein cholesterol(LDL-C) and apolipoprotein (Apo) AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 73.5%and 26.5%in Bai Ku Yao,and 67.9%and 32.1%in Han(P【0.01).The frequency of CC,CT and TT genotypes was 50.4%,46.2%and 3.4%in Bai Ku Yao,and 41.4%, 53.1%and 5.5%in Han(P【0.01);respectively.Serum HDL-C levels in both ethnic groups were different among the three genotypes(P【0.05 for each).Serum TC levels in both ethnic groups were also different between CC and CT/TT types(P【 0.05 for each).The T allele carriers had higher serum HDL-C and TC levels than the T allele noncarriers.Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotype in Bai Ku Yao(P【 0.05 for each),whereas the levels of TC and HDL-C were associated with genotype in Han Chinese(P【0.05 and P【 0.01).Serum lipid parameters were also correlated with gender, age,weight,body massindex,alcohol consumption, cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The frequency of LIPG 584Tallele is lower in the Bai Ku Yao than in the Han populations.The LIPG 584Tallele is associated with serum HDL-C and TC levels.The differences in serum lipid profiles between the two ethnic groups might partly result from different genotypic and allelic of LIPG 584C 】T or different LIPG-enviromental interactions.

Serum lipid levels and their risk factors in theGuangxi Mu Lao Zu population

Objectives Mu Lao Zu population.Methods A total of 1055 subjects of Mu Lao Zu were studied by a stratified randomized cluster sampling.Blood pressure,body height, body weight,waist circumference and serum lipid and apolipoprotein(Apo) levels were measured.The data were compared with those in 969 subjects of Han Chinese from the same region.In order to evaluate the association of hyperlipidemia with nine possible riks factors,multivariate logistic regression analysis was also performed in the combined population of Mu Lao Zu and Han,Mu Lao Zu,and Han;respectively. Results The levels of total cholesterol,triglyceride, low-density lipoprotein cholesterol and ApoB in Mu Lao Zu were significantly higher than those in Han(P【0.05-0.001); but the levels of high-density lipoprotein cholesterol,ApoA1 and ApoA1/Apo were significantly lower than those in Han (P【0.001 ).The prevalence of hyperlipidemia and Hypercholesterolemia was also significantly higher in Mu Lao Zu than in Han(53.0%vs.46.1%,P【0.001) and(44.5%vs. 37.5%,P【0.001).There was no difference in the prevalence of hypertriglyceridemia between the both ethnic groups (22.8%vs.19.4%,P】0.05).Hyperlipidemia was positively correlated with age,BMI,and systolic blood pressureMu Lao Zu(P【0.05),whereas it was positively correlated with BMI and hypertension in Han(P【0.05 for each).Conclusions The serum lipid profiles,the prevalence of hyperlipidemia and the risk factors are different between the Mu Lao Zu and Han populations.The differences in serum lipid profiles between the two ethnic groups might result from different dietary habit,life style,as well as genetic factors,in ? To determine the serum lipid levels and their risk factors in the Guangxi.

The effects of consumption of raw garlic on serum lipid level, blood sugar and a number of effective hormones on lipid and sugar metabolism in hyperglycemic and/or hyperlipidemic individuals ——Benefit of raw garlic consumption

Diabetes mellitus is one of the worldwide largest growing metabolic diseases. In this study the effects of consumption of raw garlic on serum lipid level, blood sugar and a number of effective hormones on lipid and sugar metabolism (thyroid hormones and insulin) in individuals either with high level of blood sugar or lipid were investigated. Eighty five volunteers having following clinical history enrolled in the study: Group 1: having blood sugar more than 126 mg/dl (30 vo-lunteers);Group 2: having cholesterol higher than 245 mg/dl (30 volunteers);Group 3: having blood sugar over 126 mg/dl and cholesterol more than 245 mg/dl (25 volunteers). In group 1, there wasn’t a sig-nificant differences between biochemical factors be-fore and after garlic consumption, whereas, in the second group after 6 weeks of garlic consumption a decline in cholesterol (P < 0.001), FBS and TG (P < 0.01) was observed but HDL-C (P < 0.001) was in-creased. Six weeks after termination of garlic con-sumption, cholesterol (P < 0.001), FBS and TG (P < 0.05) were in- creased and HDL-C (P < 0.01) was de-creased. In the third group, total cholesterol (P < 0.001), FBS (P < 0.01) and HbA1c decreased while HDL-C was in- creased. Other factors, including thy-roid hormones (T3, T4), TSH and insulin showed no significant alte- ration. In conclusion our results shown that raw garlic consumption can reduce the FBS and Lipid in hyperglycemic and/or hyperlipidemic individuals.

Review on article of effects of tenofovir alafenamide and entecavir in chronic hepatitis B virus patients

This letter comments on the article which reported that tenofovir alafenamide may increase blood lipid levels compared with entecavir in patients with chronic hepatitis B published on World J Hepatol 2023 August 27.We review the related research content,topic selection,methodology,conclusions,strengths and weaknesses of this article.And evaluate it in relation to other published relevant articles.

司美格鲁肽在2型糖尿病患者中的心血管保护作用研究

目的 探究司美格鲁肽在2型糖尿病(Type 2 Diabetes, T2DM)患者中的心血管保护作用。方法 选取2023年1—10月广东省佛山市顺德明景糖尿病医院接受治疗的90例T2DM患者作为研究对象,根据随机数表法分为对照组和观察组,每组45例,对照组采用二甲双胍治疗,观察组采用二甲双胍联合司美格鲁肽治疗。对比不同治疗方式的应用疗效。结果 治疗后,观察组血糖指标低于对照组,差异有统计学意义(P<0.05);治疗后,观察组总胆固醇(3.81±0.85)mmol/L、低密度脂蛋白胆固醇(2.47±0.19)mmol/L低于对照组,差异有统计学意义(t=2.693、2.991,P均<0.05);治疗后,观察组炎性因子水平低于对照组,差异有统计学意义(P<0.05)。结论 T2DM患者治疗中,在二甲双胍基础上加入司美格鲁肽,可稳定血糖及血脂情况,优化血清炎性因子水平,具有心血管保护作用。

青少年抑郁症自伤自杀行为与血脂水平及甲状腺功能的关系

目的:探讨青少年抑郁症患者自伤自杀行为与血脂水平及甲状腺功能的相关性。方法:纳入2017年7月至2020年7月西安交通大学第一附属医院住院青少年抑郁症患者230例,收集其一般资料,检测其血脂4项及甲状腺功能3项,比较自伤自杀组和无自伤自杀组各项指标,采用Logistic回归方法分析自伤自杀组的影响因素。结果:与无自伤自杀行为组相比,青少年抑郁症女性、非独生子女家庭、病程较长易出现自伤自杀行为(P<0.05),其血脂中高密度脂蛋白(HDL)水平较低(P<0.05)。两组游离三碘甲状腺原氨酸、游离甲状腺素及促甲状腺激素水平均无统计学意义。结论:青少年抑郁症患者的自伤自杀行为与女性、病程长、人际关系差及HDL水平有关。

依折麦布联合瑞舒伐他汀钙对老年冠心病伴高血脂症患者的临床疗效及心血管功能的影响

目的:探究依折麦布联合瑞舒伐他汀钙对老年冠心病伴高血脂症患者的临床效果及心血管功能的影响。方法:选取120例老年冠心病伴高血脂症的患者为研究对象,根据治疗方式不同分为对照组和观察组,每组各60例。对照组患者采用瑞舒伐他汀钙治疗;观察组患者采用瑞舒伐他汀钙联合依折麦布治疗,疗程均为8周。比较两组患者临床疗效[颈动脉内膜-中层厚度(IMT)、斑块厚度(PT)、斑块数量(PN)]、血脂水平[总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)]、血管内皮细胞功能[血管内皮生长因子(VEGF)、内皮素1(ET-1)、一氧化氮(NO)]、心功能[左心室舒张末期内经(LVDD)、左心室射血分数(LVEF)、心排血量]及不良反应发生情况。结果:治疗后,两组患者IMT、PT、PN均减小(P<0.05),且观察组小于对照组(P<0.05);TC、TG、LDL-C、VEGF、ET-1及LVDD水平均降低(P<0.05),且观察组低于对照组(P<0.05);HDL-C、NO、LVEF水平及心排血量均升高(P<0.05),且观察组高于对照组(P<0.05)。两组患者治疗期间不良反应发生情况比较,差异无统计学意义(P>0.05)。结论:依折麦布联合瑞舒伐他汀钙治对老年冠心病伴有高血脂症效果明显,可调节血脂水平,改善血管内皮功能,增强心功能,安全性好。

人外周血脂质水平和炎症指标与原发性开角型青光眼的关联性

目的探讨外周血脂质水平和炎症指标与原发性开角型青光眼(POAG)的相关性。方法采用病例对照研究, 收集2016年1月至2022年3月于汕头大学·香港中文大学联合汕头国际眼科中心确诊为POAG的86例患者作为POAG组, 同时收集年龄、性别、体质量指数相匹配的86例单纯年龄相关性白内障患者作为对照组。采用Goldmann压平式眼压计测量眼压;采用Humphrey视野分析仪测量视野平均缺损(MD)。采集各组受试者外周静脉血, 使用全自动生化分析仪检测总胆固醇、总甘油三酯、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和C反应蛋白表达水平。使用全自动血液分析仪检测白细胞、中性粒细胞、淋巴细胞、单核细胞表达水平。筛选2个组间差异有统计学意义的指标作为自变量, 采用多重Logistic回归分析POAG的危险因素。采用Pearson线性相关分析确定各危险因素与POAG患眼眼压及MD之间的相关性。结果 POAG组HDL水平为(1.59±0.42)mmol/L, 明显低于对照组的(1.76±0.50)mmol/L, 差异有统计学意义(t=2.435, P=0.016)。POAG组LDL水平为(3.34±0.66)mmol/L, 明显高于对照组的(3.08±0.71)mmol/L, 差异有统计学意义(t=2.520, P=0.013)。POAG组白细胞水平为(6.91±1.60)×109/L, 明显高于对照组的(6.11±1.29)×109/L, 差异有统计学意义(t=3.619, P<0.001)。多重Logistic回归分析结果显示, 外周血LDL水平升高(P=0.039, OR=2.354, 95%CI:1.105~5.303)和白细胞水平升高(P=0.044, OR=1.310, 95%CI:1.007~1.703)是POAG发病的危险因素。Pearson相关分析发现, POAG患者外周血LDL和白细胞水平均与眼压呈中等强度正相关(r=0.610, P<0.001;r=0.358, P=0.001);LDL水平与MD呈中等强度负相关(r=-0.496, P<0.001), 白细胞水平与MD呈弱负相关(r=-0.235, P=0.030)。结论外周血LDL和白细胞水平升高是POAG患病的危险因素, 并与POAG患者眼压呈正相关, 与MD呈负相关。

五子衍宗方散剂对罗曼蛋鸡生产性能和血清生化指标的影响

以130日龄罗曼蛋鸡为研究对象,在其基础日粮中添加0(CK)、0.5%(Ⅰ组)、1.0%(Ⅱ组)、1.5%(Ⅲ组)、2.0%(Ⅳ组)、2.5%(Ⅴ组)的五子衍宗方散剂,饲喂68 d,研究饲粮中添加不同水平五子衍宗方散剂对产蛋初期罗曼蛋鸡生产性能、血液生化指标的影响。结果表明:与CK相比,各试验组的平均日采食量以及蛋鸡血清中总蛋白、白蛋白、球蛋白、谷丙转氨酶、总胆红素、直接胆红素、间接胆红素含量均无显著差异;但I组能够显著降低蛋鸡血清中肌酐、尿酸和总胆汁酸的含量,且对血清中碱性磷酸酶、谷草转氨酶、乳酸脱氢酶含量无显著影响;I~Ⅱ组能够显著降低试验蛋鸡料蛋比并显著提高试验蛋鸡全期产蛋率;I~Ⅲ组均能显著降低蛋鸡血清中总胆固醇、甘油三酯和低密度脂蛋白胆固醇含量;而Ⅲ~Ⅴ组蛋鸡血清中肌酐含量显著升高;Ⅴ组能够显著提高血清中谷草转氨酶、碱性磷酸酶、乳酸脱氢酶含量,Ⅱ~Ⅳ组血清中碱性磷酸酶含量显著升高。综上所述,在基础日粮中添加0.5%的五子衍宗方散剂可改善产蛋初期罗曼蛋鸡的生产性能、血清生化指标,并调节蛋鸡血脂代谢,但添加1.0%~2.5%的五子衍宗方散剂会给蛋鸡带来不利影响。综合考虑,产蛋初期罗曼蛋鸡饲粮中五子衍宗方散剂添加量应以0.5%为宜。

葛根素对膳食诱导的高胆固醇血症大鼠的血脂调节作用

目的:观察葛根素对膳食诱导的高胆固醇血症大鼠的血脂调节作用,并对其机制进行探索。方法:SD大鼠随机分为3组,正常组、模型组和葛根素组。模型组和葛根素组均给予富含胆固醇的饲料4周,葛根素组每天灌喂葛根素溶液(300mg·kg-1·d-1)。检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-c)和低密度脂蛋白胆固醇(LDL-c)的含量。荧光定量RT-PCR分析羟基-3-甲基戊二酰CoA还原酶(HMGR),羊毛固醇14α-去甲基酶(CYP51),7α-羟化酶(CYP7A1)的mRNA转录水平。结果:葛根素显著降低高胆固醇膳食引起的血清胆固醇的升高,降低动脉粥样硬化指数,上调CYP7A1的mRNA转录水平,对HMGR和CYP51的转录水平没有影响。结论:这些数据表明葛根素能够降低血清胆固醇,减低动脉粥样硬化发生危险。其降低胆固醇的作用可能是通过促进胆固醇转化为胆酸实现的。

DHA油与其他4种油脂对大鼠空间学习记忆、脂质过氧化及其血脂水平的影响

研究DHA油和紫苏油、核桃油、花生油、菜籽油对大鼠空间学习记忆、脂质过氧化及其血脂水平的影响。将88只初断乳SD大鼠分为11组,每组8只,5种油各设置2.445 mL/kg和8.15 mL/kg两个剂量组,另设一个阴性对照组,样品经口灌胃30 d。Morris水迷宫进行大鼠行为学测试,并测定脑组织和血清生化指标。结果表明:与阴性对照组相比,紫苏油和核桃油各剂量组逃避潜伏期均明显缩短(P<0.05,P<0.01),紫苏油大剂量组、核桃油大剂量组脑组织的乙酰胆碱酯酶活力明显下降(P<0.01,P<0.05),紫苏油大剂量组、花生油组、菜籽油大剂量组超氧化物歧化酶活力均明显增强,DHA油大剂量组超氧化物歧化酶活力、丙二醛含量均明显升高,DHA油大、小剂量组血清总胆固醇含量均明显降低,花生油大、小剂量组谷丙转氨酶显著升高;紫苏油、核桃油可以增强大鼠的空间学习记忆能力;紫苏油、花生油、菜籽油具有一定的抗氧化作用;DHA油可以降低血脂,但剂量过大会造成肝脏脂质过氧化水平升高;花生油可能会引起肝功能指标谷丙转氨酶异常。

广州市457名在校学生肥胖和血脂紊乱状况分析

目的分析广州市457名在校学生肥胖和血脂紊乱状况。方法随机抽取广州市区小学、初中、高中各一所学校的学生共457名,测量身高、体重、脂肪含量,采集静脉血测定总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG),按照美国国家胆固醇教育计划(NCEP)推荐的儿童血脂异常诊断标准进行分析。中国学龄儿童青少年超重、肥胖筛查体重指数依据中国肥胖问题工作组推荐标准。结果该样本中31.1出现血脂紊乱,超重+肥胖率为36.3,肥胖率为15.8;血脂紊乱发生率在体型正常者中与超重或肥胖者中有显著性差异(P<0.05);TC和脂肪含量在男女性别之间总体均数差异有统计学意义(P<0.05);体重指数(BMI)除LDL-C外,与TG、TC、HDL-C相关系数差异有统计学意义(P<0.05)。结论此次调查结果显示,457名学生中,按照青少年肥胖分级,超重与肥胖率已达到Ⅲ级水平中的高度警戒水平。

茶多酚对肉鸡血脂水平、体脂分布及组织脂肪酸组成的影响

本文以28日龄雄性白羽肉鸡为研究对象,在正常饲喂基础上分别每天逐只灌喂80 mg·kg^(-1)和160 mg·kg^(-1)体重的茶多酚,以考察实验处理对肉鸡脂肪代谢和组织脂肪酸组成的影响,实验周期为28 d。结果表明,茶多酚处理能够显著降低肉鸡腹脂率、皮下脂肪厚度和肌间脂肪宽度,并改善动物的血脂水平。实验处理组动物肝脏、腿肌和胸肌含脂率与对照组动物相比显著下降,肝脏和腿肌单不饱和脂肪酸比例显著下降,而多不饱和脂肪酸比例显著升高。与对照组动物相比,160 mg·kg^(-1)茶多酚组肉鸡肝脏花生四烯酸(C20∶4)、二十二碳五烯酸(C22∶5)和二十二碳六烯酸(C22∶6)分别显著提高50.5%(p<0.001)、32.8%(p<0.05)和48.4%(p<0.05),而腿肌中该三种多不饱和脂肪酸分别升高83.7%(p<0.01)、86.0%(p<0.01)和78.3%(p<0.05)。因此,茶多酚处理可显著改善肉鸡的血脂水平和体脂分布,并在一定程度上提高腿肌的营养价值。

天津市居民血脂水平与非膳食因素关系的研究

报道了天津市１９９２年随机抽调６３１名１５～６４岁城乡居民的血脂水平。结果发现：天津市居民总胆固醇（ＴＣ）男性４．０８ｍｍｏｌ／Ｌ，女性３．９４ｍｍｏｌ／Ｌ；甘油三酯（ＴＧ）男性１．４８ｍｍｏｌ／Ｌ，女性１．２０ｍｍｏｌ／Ｌ；高密度脂蛋白胆固醇（ＨＤＬ）男性１．２４ｍｍｏｌ／Ｌ，女性１．３５ｍｍｏｌ／Ｌ。ＴＧ随年龄增长而上升，女性ＨＤＬ显著高于男性。血脂水平与体质指数（ＢＭＩ）及文化程度相关，ＢＭＩ越大血脂水平越高，而文化程度与ＴＧ水平呈负相关。

载脂蛋白E基因多态性与老年人群血脂的研究

目的观察载脂蛋白E(ApoE)的遗传多态性与老年人血脂的关系。方法808例受试者,其中脑卒中430例、冠心病167例及对照组211例。采用聚合酶链式反应限制性片段多态性方法检测ApoE基因多态性。结果ApoE4表型携带者的TC、LDLC和ApoB水平显著高于ApoE2表型携带者(P<005),ApoE3/3表型携带者的LDLC水平也高于ApoE2表型携带者(P<005),而ApoE2表型携带者的HDLC水平显著高于ApoE4(P<005),这种作用在男女两性中相似。结论ApoE基因多态性影响老年人群血脂水平,无性别差异。