BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PA...BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PAPP-A),triglyceride(TG),and 25-hydroxyvitamin D[25-(OH)D]with GDM in early pregnancy.METHODS A total of 99 patients in early pregnancy admitted to Peking University International Hospital from November 2015 to September 2017 were included,and underwent a fasting glucose test and oral glucose tolerance test screening at 24-28 wk of pregnancy.Of these cases with GDM,51 were assigned to group A and the remaining 48 cases without GDM were enrolled in group B.Serum PAPP-A,TG and 25-(OH)D in the two groups were compared and their correlation with blood sugar was analyzed.In addition,their diagnostic value in GDM was determined using receiver operating characteristic(ROC)curve analysis.RESULTS Group A had markedly lower serum PAPP-A and 25-(OH)D levels and a significantly higher serum TG level than group B,with statistical significance(P<0.05).Furthermore,Pearson analysis identified that PAPP-A and 25-(OH)D levels were negatively correlated with fasting blood glucose(FBG)levels(r=-0.605,P<0.001),(r=-0.597,P<0.001),while TG and FBG levels were positively correlated(r=0.628,P<0.001).The sensitivity,specificity,area under the curve(AUC)and optimal cut-off value of serum PAPP-A level in the diagnosis of GDM were 72.55%,82.35%,0.861 and 16.340,respectively,while the sensitivity of TG in diagnosing GDM was 86.27%,the specificity was 66.67%,the AUC was 0.813,with an optimal cut-off value of 1.796.The corresponding sensitivity,specificity,AUC and optimal cut-off value of serum 25-(OH)D were 64.71%,70.59%,0.721 and 23.140,respectively.Moreover,multivariate logistic regression analysis revealed that FBG,vascular endothelial growth factor,Flt-1,serum PAPP-A,TG,and 25-(OH)D were related risk factors leading to GDM in patients.CONCLUSION Serum PAPP-A,TG,and 25-(OH)D levels are all correlated with blood glucose changes in GDM,and are independent factors affecting the occurrence of GDM and have certain value in the diagnosis of GDM.展开更多
Background: There is great interest in developing blood-based biomarkers for Alzheimer’s disease (AD);however, there is no consensus as to what blood fraction is most appropriate for analyzing particular markers. The...Background: There is great interest in developing blood-based biomarkers for Alzheimer’s disease (AD);however, there is no consensus as to what blood fraction is most appropriate for analyzing particular markers. The current study provides empirical evidence regarding how blood-based proteins vary depending on whether they are assayed in serum or plasma. Methods: Weanalyzed concentrations of 100 proteins in matched samples of serum and plasma from 39 Caucasian AD participants from the Texas Alzheimer’s Research and Care Consortium bymultiplex immunoassay. Results: Concentrations of 40 proteins were highly correlated (r2≥ 0.75) between plasma and serum while the remaining proteins were moderately to weakly correlated (r2< 0.75). Discussion: Whether plasma vs. serum is assayed can have a large impact on the observed concentration of some proteins, including several proteins that are of great interest to AD pathophysiology. The current findings may explain the significant discrepancies often times reported in the AD biomarker field.展开更多
In public health studies limited volumes of blood are often collected and stored for future hypothesis testing. Archived samples are irreplaceable and therefore it is valuable to develop analytical techniques that req...In public health studies limited volumes of blood are often collected and stored for future hypothesis testing. Archived samples are irreplaceable and therefore it is valuable to develop analytical techniques that require minimal sample vo-lume. This work describes the measurement of trace elements Mg, Cu, Fe, Zn and ultratrace elements Cd, Co, Mn, Pb in limited quantity (150 μL) human serum or plasma samples. Samples were digested using a hotblock and analyzed using inductively coupled plasma mass spectrometry (ICP-MS). The analytical method was evaluated using a quadrupole (Q) and sector field high resolution (SF) instrument to analyze trace elements in Seronorm? quality control serum material. The method was used to analyze 1888 blinded human plasma samples which were archived for the National Cancer Institute from the Nutrition Intervention Trial in Linxian China. The inductively coupled plasma method was capable of accurately analyzed limited quantity samples of human serum and plasma for the trace elements Mg, Cu, Fe Zn and the ultra trace elements Co, Mn and Pb. The concentration of Cd in human plasma samples was below the level of detection for 75% of the samples analyzed.展开更多
Platelet-free plasma of human blood (sodium citrate and EDTA as an anticoagulant) and serum were stored at 4°C, room temperature (25°C) and at 37°C for 24 hours. RBC aggregation decreased after incubati...Platelet-free plasma of human blood (sodium citrate and EDTA as an anticoagulant) and serum were stored at 4°C, room temperature (25°C) and at 37°C for 24 hours. RBC aggregation decreased after incubation of plasma and serum at 37°C for 4 hours. The RBC shape was changed at the same time: discocytes transformed to echinocytes. Storage of plasma and serum at 4°C and room temperature did not lead to significant alterations of RBC aggregation. The RBC shape did not change in influence of such plasma and serum. The most considerable decrease of RBC aggregation and change of their shapes were observed in the plasma and serum incubated at 37°C for 24 hours. Dilution of incubated plasma by fresh plasma led to consistent restoration of erythrocyte shape and their aggregation.展开更多
Life-saving procedures of an emergency protocol to combat fatal ambushes of viral or bacterial pathogens are listed below for immediate dissemination and implementation. This unique protocol of serum therapy commands ...Life-saving procedures of an emergency protocol to combat fatal ambushes of viral or bacterial pathogens are listed below for immediate dissemination and implementation. This unique protocol of serum therapy commands the highest benefits-versus-risks ratio, and is safe, efficacious, user-friendly and inexpensive to implement. A formal evaluation is presented to support upgrading convalescent plasma therapy to serum therapy in the context of presenting fewer donor antigens, thus evoking less lethal sensitization to obtain greater safety and efficacy in treatment.展开更多
Systemic lupus erythematosus-related acute pancreatitis（SLEAP） has a poor prognosis with a high mortality. We described the clinical features of SLEAP, and discussed the feasibility of plasma exchange（PE） combined...Systemic lupus erythematosus-related acute pancreatitis（SLEAP） has a poor prognosis with a high mortality. We described the clinical features of SLEAP, and discussed the feasibility of plasma exchange（PE） combined with glucocorticosteroids（GC） in short-term prognosis and possible mechanism in reducing serum inflammatory cytokine IL-6 and removing serum lipids. A retrospective study was performed by an independent rheumatologist. Medical records of SLEAP from March 2010 to December 2014 were retrieved from Tongji Hospital information system, and patients were divided into two groups according to whether PE therapy was adopted. Sixteen patients treated with PE in combination with GC were classified as group A, and the other 10 patients who were treated with merely GC were classified as group B. Patients＇ clinical remission rate and average daily GC dosage after two-week therapy were compared between the two groups. Patients＇ serum inflammatory cytokines and lipid concentration were compared between baseline and after two-week treatment in both groups. Pearson correlation test was performed to determine association between serum cytokines and Ranson score. SLEDAI score in group A patients at baseline（14.8±3.1） showed no statistical difference from that in group B（14.1±3.3）. At baseline serum IL-6 levels had no significant difference between group A [13.14（11.12, 16.57） mg/L] and group B [14.63（11.37, 16.37） mg/L]; after two-week therapy IL-6 decreased significantly in group A [9.16（7.93, 10.75）mg/L] while it did not show decreasing trend in group B [13.62（9.29,17.63） mg/L]. Serum lipid concentration after two-week therapy in group A [（TC=5.02±0.53, TG=1.46±0.44） mmol/L] decreased significantly compared to baseline [（TC=6.11±0.50, TG=2.14±1.03） mmol/L], while similar tendency was not observed in group B. The remission rate after two-week therapy was higher in group A（70.0%） than in group B（25.0%）. Acute pancreatitis（AP） was one of the clinical manifestations of active SLE. PE combined with GC could reduce serum IL-6 level, and remove serum lipid to improve short-term prognosis. Therefore, it might be a safe and effective way in treating SLEAP and was worth continuing to explore its feasibility.展开更多
目的调查中国两广地区法定单采血浆区域新献血浆者的血尿酸和血脂水平状况,并分析其影响因素。方法通过完全随机抽样的方式随机选取2021年4月—2022年8月来自广西内陆地区(巴马和平果)和广东沿海地区(遂溪和廉江)4家单采血浆公司的合计1...目的调查中国两广地区法定单采血浆区域新献血浆者的血尿酸和血脂水平状况,并分析其影响因素。方法通过完全随机抽样的方式随机选取2021年4月—2022年8月来自广西内陆地区(巴马和平果)和广东沿海地区(遂溪和廉江)4家单采血浆公司的合计11485名新献血浆者,检测其血尿酸、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、总胆固醇和甘油三酯水平,并对检测结果进行统计分析。结果11485名新献血浆者中血尿酸异常者占比为22%,血脂异常率为57.6%;其中UA、LDL-C、TC及TG水平与新献血浆者年龄显著相关(UA:r_(全部)=‒0.291,r_(男性)=‒0.138,r_(女性)=‒0.170;r_(LDL-C)=0.300,r_(TC)=0.340,r_(TG)=0.234;P<0.001);UA、HDL-C、LDL-C、TC及TG水平与新献血浆者BMI显著相关(UA:r_(全部)=0.201,r_(男性)=0.243,r_(女性)=0.241;r_(HDL-C)=-0.273,r_(LDL-C)=0.316,r_(TC)=0.275,r_(TG)=0.335;P<0.001);不同献浆区域间的新献浆者血尿酸(UA)和血(HDL-C,LDL-C,TC,TG)水平均存在显著差异(P<0.001);不同血型新献血浆者间的HDL-C、LDL-C和TC水平存在显著差异(P<0.001);新献血浆者中男性的UA和TG水平显著高于女性(UA:366μmol/L v s 262μmol/L,TG:1.4 mmol/L vs 1.4 mmol/L;P<0.001),女性新献血浆者的HDL-C和TC水平显著高于男性(HDL-C:1.1 mmol/L vs 0.9 mmol/L,TC:3.7 mmol/L vs 3.6 mmol/L;P<0.001);汉族和少数民族新献血浆者间的UA、HDL-C和TC水平存在显著差异(P<0.001);不同地区、性别、血型和民族的新献血浆者间血尿酸和血脂异常率存在显著差异(P<0.001);新献血浆者的浆站区域、血脂异常情况、性别、年龄、体重及身高是其血尿酸异常的独立风险因素(P<0.001);新献血浆者的浆站区域、血尿酸异常、性别、血型、年龄、体重及身高是其血脂异常的独立风险因素(P<0.001);新献血浆者的浆站区域、性别、体重及身高是其血尿酸和血脂异常的独立风险因素(P<0.001)。结论中国两广地区新献血浆者的血尿酸和血脂异常率较高,其中广东沿海地区的新献血浆者上述指标异常率较广西内陆地区比例高。推测这可能与两地不同的饮食习惯密切相关。展开更多
文摘BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PAPP-A),triglyceride(TG),and 25-hydroxyvitamin D[25-(OH)D]with GDM in early pregnancy.METHODS A total of 99 patients in early pregnancy admitted to Peking University International Hospital from November 2015 to September 2017 were included,and underwent a fasting glucose test and oral glucose tolerance test screening at 24-28 wk of pregnancy.Of these cases with GDM,51 were assigned to group A and the remaining 48 cases without GDM were enrolled in group B.Serum PAPP-A,TG and 25-(OH)D in the two groups were compared and their correlation with blood sugar was analyzed.In addition,their diagnostic value in GDM was determined using receiver operating characteristic(ROC)curve analysis.RESULTS Group A had markedly lower serum PAPP-A and 25-(OH)D levels and a significantly higher serum TG level than group B,with statistical significance(P<0.05).Furthermore,Pearson analysis identified that PAPP-A and 25-(OH)D levels were negatively correlated with fasting blood glucose(FBG)levels(r=-0.605,P<0.001),(r=-0.597,P<0.001),while TG and FBG levels were positively correlated(r=0.628,P<0.001).The sensitivity,specificity,area under the curve(AUC)and optimal cut-off value of serum PAPP-A level in the diagnosis of GDM were 72.55%,82.35%,0.861 and 16.340,respectively,while the sensitivity of TG in diagnosing GDM was 86.27%,the specificity was 66.67%,the AUC was 0.813,with an optimal cut-off value of 1.796.The corresponding sensitivity,specificity,AUC and optimal cut-off value of serum 25-(OH)D were 64.71%,70.59%,0.721 and 23.140,respectively.Moreover,multivariate logistic regression analysis revealed that FBG,vascular endothelial growth factor,Flt-1,serum PAPP-A,TG,and 25-(OH)D were related risk factors leading to GDM in patients.CONCLUSION Serum PAPP-A,TG,and 25-(OH)D levels are all correlated with blood glucose changes in GDM,and are independent factors affecting the occurrence of GDM and have certain value in the diagnosis of GDM.
文摘Background: There is great interest in developing blood-based biomarkers for Alzheimer’s disease (AD);however, there is no consensus as to what blood fraction is most appropriate for analyzing particular markers. The current study provides empirical evidence regarding how blood-based proteins vary depending on whether they are assayed in serum or plasma. Methods: Weanalyzed concentrations of 100 proteins in matched samples of serum and plasma from 39 Caucasian AD participants from the Texas Alzheimer’s Research and Care Consortium bymultiplex immunoassay. Results: Concentrations of 40 proteins were highly correlated (r2≥ 0.75) between plasma and serum while the remaining proteins were moderately to weakly correlated (r2< 0.75). Discussion: Whether plasma vs. serum is assayed can have a large impact on the observed concentration of some proteins, including several proteins that are of great interest to AD pathophysiology. The current findings may explain the significant discrepancies often times reported in the AD biomarker field.
文摘In public health studies limited volumes of blood are often collected and stored for future hypothesis testing. Archived samples are irreplaceable and therefore it is valuable to develop analytical techniques that require minimal sample vo-lume. This work describes the measurement of trace elements Mg, Cu, Fe, Zn and ultratrace elements Cd, Co, Mn, Pb in limited quantity (150 μL) human serum or plasma samples. Samples were digested using a hotblock and analyzed using inductively coupled plasma mass spectrometry (ICP-MS). The analytical method was evaluated using a quadrupole (Q) and sector field high resolution (SF) instrument to analyze trace elements in Seronorm? quality control serum material. The method was used to analyze 1888 blinded human plasma samples which were archived for the National Cancer Institute from the Nutrition Intervention Trial in Linxian China. The inductively coupled plasma method was capable of accurately analyzed limited quantity samples of human serum and plasma for the trace elements Mg, Cu, Fe Zn and the ultra trace elements Co, Mn and Pb. The concentration of Cd in human plasma samples was below the level of detection for 75% of the samples analyzed.
文摘Platelet-free plasma of human blood (sodium citrate and EDTA as an anticoagulant) and serum were stored at 4°C, room temperature (25°C) and at 37°C for 24 hours. RBC aggregation decreased after incubation of plasma and serum at 37°C for 4 hours. The RBC shape was changed at the same time: discocytes transformed to echinocytes. Storage of plasma and serum at 4°C and room temperature did not lead to significant alterations of RBC aggregation. The RBC shape did not change in influence of such plasma and serum. The most considerable decrease of RBC aggregation and change of their shapes were observed in the plasma and serum incubated at 37°C for 24 hours. Dilution of incubated plasma by fresh plasma led to consistent restoration of erythrocyte shape and their aggregation.
文摘Life-saving procedures of an emergency protocol to combat fatal ambushes of viral or bacterial pathogens are listed below for immediate dissemination and implementation. This unique protocol of serum therapy commands the highest benefits-versus-risks ratio, and is safe, efficacious, user-friendly and inexpensive to implement. A formal evaluation is presented to support upgrading convalescent plasma therapy to serum therapy in the context of presenting fewer donor antigens, thus evoking less lethal sensitization to obtain greater safety and efficacy in treatment.
基金supported by a grant of Innovation Talents Development Fund of Wuhan Municipal Human Resources and Social Security Bureau,China(No.201402)
文摘Systemic lupus erythematosus-related acute pancreatitis（SLEAP） has a poor prognosis with a high mortality. We described the clinical features of SLEAP, and discussed the feasibility of plasma exchange（PE） combined with glucocorticosteroids（GC） in short-term prognosis and possible mechanism in reducing serum inflammatory cytokine IL-6 and removing serum lipids. A retrospective study was performed by an independent rheumatologist. Medical records of SLEAP from March 2010 to December 2014 were retrieved from Tongji Hospital information system, and patients were divided into two groups according to whether PE therapy was adopted. Sixteen patients treated with PE in combination with GC were classified as group A, and the other 10 patients who were treated with merely GC were classified as group B. Patients＇ clinical remission rate and average daily GC dosage after two-week therapy were compared between the two groups. Patients＇ serum inflammatory cytokines and lipid concentration were compared between baseline and after two-week treatment in both groups. Pearson correlation test was performed to determine association between serum cytokines and Ranson score. SLEDAI score in group A patients at baseline（14.8±3.1） showed no statistical difference from that in group B（14.1±3.3）. At baseline serum IL-6 levels had no significant difference between group A [13.14（11.12, 16.57） mg/L] and group B [14.63（11.37, 16.37） mg/L]; after two-week therapy IL-6 decreased significantly in group A [9.16（7.93, 10.75）mg/L] while it did not show decreasing trend in group B [13.62（9.29,17.63） mg/L]. Serum lipid concentration after two-week therapy in group A [（TC=5.02±0.53, TG=1.46±0.44） mmol/L] decreased significantly compared to baseline [（TC=6.11±0.50, TG=2.14±1.03） mmol/L], while similar tendency was not observed in group B. The remission rate after two-week therapy was higher in group A（70.0%） than in group B（25.0%）. Acute pancreatitis（AP） was one of the clinical manifestations of active SLE. PE combined with GC could reduce serum IL-6 level, and remove serum lipid to improve short-term prognosis. Therefore, it might be a safe and effective way in treating SLEAP and was worth continuing to explore its feasibility.
文摘目的调查中国两广地区法定单采血浆区域新献血浆者的血尿酸和血脂水平状况,并分析其影响因素。方法通过完全随机抽样的方式随机选取2021年4月—2022年8月来自广西内陆地区(巴马和平果)和广东沿海地区(遂溪和廉江)4家单采血浆公司的合计11485名新献血浆者,检测其血尿酸、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、总胆固醇和甘油三酯水平,并对检测结果进行统计分析。结果11485名新献血浆者中血尿酸异常者占比为22%,血脂异常率为57.6%;其中UA、LDL-C、TC及TG水平与新献血浆者年龄显著相关(UA:r_(全部)=‒0.291,r_(男性)=‒0.138,r_(女性)=‒0.170;r_(LDL-C)=0.300,r_(TC)=0.340,r_(TG)=0.234;P<0.001);UA、HDL-C、LDL-C、TC及TG水平与新献血浆者BMI显著相关(UA:r_(全部)=0.201,r_(男性)=0.243,r_(女性)=0.241;r_(HDL-C)=-0.273,r_(LDL-C)=0.316,r_(TC)=0.275,r_(TG)=0.335;P<0.001);不同献浆区域间的新献浆者血尿酸(UA)和血(HDL-C,LDL-C,TC,TG)水平均存在显著差异(P<0.001);不同血型新献血浆者间的HDL-C、LDL-C和TC水平存在显著差异(P<0.001);新献血浆者中男性的UA和TG水平显著高于女性(UA:366μmol/L v s 262μmol/L,TG:1.4 mmol/L vs 1.4 mmol/L;P<0.001),女性新献血浆者的HDL-C和TC水平显著高于男性(HDL-C:1.1 mmol/L vs 0.9 mmol/L,TC:3.7 mmol/L vs 3.6 mmol/L;P<0.001);汉族和少数民族新献血浆者间的UA、HDL-C和TC水平存在显著差异(P<0.001);不同地区、性别、血型和民族的新献血浆者间血尿酸和血脂异常率存在显著差异(P<0.001);新献血浆者的浆站区域、血脂异常情况、性别、年龄、体重及身高是其血尿酸异常的独立风险因素(P<0.001);新献血浆者的浆站区域、血尿酸异常、性别、血型、年龄、体重及身高是其血脂异常的独立风险因素(P<0.001);新献血浆者的浆站区域、性别、体重及身高是其血尿酸和血脂异常的独立风险因素(P<0.001)。结论中国两广地区新献血浆者的血尿酸和血脂异常率较高,其中广东沿海地区的新献血浆者上述指标异常率较广西内陆地区比例高。推测这可能与两地不同的饮食习惯密切相关。
