BACKGROUND Necrotising enterocolitis(NEC)is a critical gastrointestinal emergency affecting premature and low-birth-weight neonates.Serum amyloid A(SAA),procalcitonin(PCT),and high-mobility group box 1(HMGB1)have emer...BACKGROUND Necrotising enterocolitis(NEC)is a critical gastrointestinal emergency affecting premature and low-birth-weight neonates.Serum amyloid A(SAA),procalcitonin(PCT),and high-mobility group box 1(HMGB1)have emerged as potential biomarkers for NEC due to their roles in inflammatory response,tissue damage,and immune regulation.AIM To evaluate the diagnostic value of SAA,PCT,and HMGB1 in the context of NEC in newborns.METHODS The study retrospectively analysed the clinical data of 48 newborns diagnosed with NEC and 50 healthy newborns admitted to the hospital.Clinical,radiological,and laboratory findings,including serum SAA,PCT,and HMGB1 Levels,were collected,and specific detection methods were used.The diagnostic value of the biomarkers was evaluated through statistical analysis,which was performed using chi-square test,t-test,correlation analysis,and receiver operating characteristic(ROC)analysis.RESULTS The study demonstrated significantly elevated levels of serum SAA,PCT,and HMGB1 Levels in newborns diagnosed with NEC compared with healthy controls.The correlation analysis indicated strong positive correlations among serum SAA,PCT,and HMGB1 Levels and the presence of NEC.ROC analysis revealed promising sensitivity and specificity for serum SAA,PCT,and HMGB1 Levels as potential diagnostic markers.The combined model of the three biomarkers demonstrating an extremely high area under the curve(0.908).CONCLUSION The diagnostic value of serum SAA,PCT,and HMGB1 Levels in NEC was highlighted.These biomarkers potentially improve the early detection,risk stratification,and clinical management of critical conditions.The findings suggest that these biomarkers may aid in timely intervention and the enhancement of outcomes for neonates affected by NEC.展开更多
A reduction in adult neurogenesis is associated with behavioral abnormalities in patients with Alzheimer's disease.Consequently,enhancing adult neurogenesis represents a promising therapeutic approach for mitigati...A reduction in adult neurogenesis is associated with behavioral abnormalities in patients with Alzheimer's disease.Consequently,enhancing adult neurogenesis represents a promising therapeutic approach for mitigating disease symptoms and progression.Nonetheless,nonpharmacological interventions aimed at inducing adult neurogenesis are currently limited.Although individual non-pharmacological interventions,such as aerobic exercise,acousto-optic stimulation,and olfactory stimulation,have shown limited capacity to improve neurogenesis and cognitive function in patients with Alzheimer's disease,the therapeutic effect of a strategy that combines these interventions has not been fully explored.In this study,we observed an age-dependent decrease in adult neurogenesis and a concurrent increase in amyloid-beta accumulation in the hippocampus of amyloid precursor protein/presenilin 1 mice aged 2-8 months.Amyloid deposition became evident at 4 months,while neurogenesis declined by 6 months,further deteriorating as the disease progressed.However,following a 4-week multifactor stimulation protocol,which encompassed treadmill running(46 min/d,10 m/min,6 days per week),40 Hz acousto-optic stimulation(1 hour/day,6 days/week),and olfactory stimulation(1 hour/day,6 days/week),we found a significant increase in the number of newborn cells(5'-bromo-2'-deoxyuridine-positive cells),immature neurons(doublecortin-positive cells),newborn immature neurons(5'-bromo-2'-deoxyuridine-positive/doublecortin-positive cells),and newborn astrocytes(5'-bromo-2'-deoxyuridine-positive/glial fibrillary acidic protein-positive cells).Additionally,the amyloid-beta load in the hippocampus decreased.These findings suggest that multifactor stimulation can enhance adult hippocampal neurogenesis and mitigate amyloid-beta neuropathology in amyloid precursor protein/presenilin 1 mice.Furthermore,cognitive abilities were improved,and depressive symptoms were alleviated in amyloid precursor protein/presenilin 1 mice following multifactor stimulation,as evidenced by Morris water maze,novel object recognition,forced swimming test,and tail suspension test results.Notably,the efficacy of multifactor stimulation in consolidating immature neurons persisted for at least 2weeks after treatment cessation.At the molecular level,multifactor stimulation upregulated the expression of neuron-related proteins(NeuN,doublecortin,postsynaptic density protein-95,and synaptophysin),anti-apoptosis-related proteins(Bcl-2 and PARP),and an autophagyassociated protein(LC3B),while decreasing the expression of apoptosis-related proteins(BAX and caspase-9),in the hippocampus of amyloid precursor protein/presenilin 1 mice.These observations might be attributable to both the brain-derived neurotrophic factor-mediated signaling pathway and antioxidant pathways.Furthermore,serum metabolomics analysis indicated that multifactor stimulation regulated differentially expressed metabolites associated with cell apoptosis,oxidative damage,and cognition.Collectively,these findings suggest that multifactor stimulation is a novel non-invasive approach for the prevention and treatment of Alzheimer's disease.展开更多
Objective: To investigate the expression level and correlation of soluble programmed death factor (sPD-L1) and vascular endothelial growth factor (VEGF) in serum of patients with acute myeloid leukemia.Methods: From J...Objective: To investigate the expression level and correlation of soluble programmed death factor (sPD-L1) and vascular endothelial growth factor (VEGF) in serum of patients with acute myeloid leukemia.Methods: From January 2016 to October 2017, 36 cases with acute myeloid leukemia were selected as AML group. After 1-2 courses of standard chemotherapy, 27 cases with complete remission were selected as complete remission group, 9 cases without improvement as a group. In the same period, 20 healthy patients were selected as the healthy control group. The expression levels of sPD-L1 and VEGF were detected by enzyme-linked immunoassay (ELISA).Results: The expression of sPD-L1 and VEGF in serum of the initial and unrelieved AML patients was significantly higher than that in the healthy control group, and the difference was statistically significant. After 1-2 courses of standard chemotherapy, the expression of sPD-L1 and VEGF in the serum of patients with complete remission was significantly lower than that in the initial treatment group, and the difference was statistically significant. There was no statistically significant difference between the total relief group and the control group. There is a significant positive correlation between the two expressions. Conclusions: The high expression of sPD-L1and VEGF in serum may be related to the occurrence, development and prognosis of AML. Therefore, the joint detection of sPD-L1 and VEGF is of some significance to the early diagnosis, monitoring efficacy and prognosis of AML patients.展开更多
目的探讨可溶性程序性死亡蛋白1(soluble programmed death protein 1,sPD-1)、可溶性程序性死亡蛋白配体1(soluble programmed death protein ligand 1,sPD-L1)在原发性肾病综合征(primary nephrotic syndrome,PNS)患者中的表达及其临...目的探讨可溶性程序性死亡蛋白1(soluble programmed death protein 1,sPD-1)、可溶性程序性死亡蛋白配体1(soluble programmed death protein ligand 1,sPD-L1)在原发性肾病综合征(primary nephrotic syndrome,PNS)患者中的表达及其临床意义。方法选择2016年4月至2019年4月我院收治的PNS患者87例作为病例组,同期在我院健康体检者87例作为对照组,比较两组血清sPD-1、sPD-L1水平,比较不同病理类型PNS患者血清sPD-1、sPD-L1水平,比较不同转归情况PNS患者血清sPD-1、sPD-L1水平,采用P earson相关分析PNS患者血清sPD-1、sPD-L1水平的相关性,采用多因素Logistic回归分析PNS发生的危险因素。结果病例组治疗前血清sPD-1、sPD-L1水平明显高于对照组,差异具有统计学意义(P<0.05);3种病理类型的PNS患者治疗前血清sPD-1、sPD-L1水平差异具有统计学意义(P<0.05);3种病理类型的PNS患者治疗后血清sPD-1、sPD-L1水平差异具有统计学意义(P<0.05);3种病理类型的PNS患者治疗前、后血清sPD-1、sPD-L1水平差异均具有统计学意义(P<0.05);不同转归情况PNS患者治疗后血清sPD-1、sPD-L1水平差异具有统计学意义(P<0.05);PNS患者血清sPD-1、sPD-L1水平呈正相关(P<0.05);sPD-1、sPD-L1是PNS的影响因素(P<0.05)。结论sPD-1、sPD-L1在原发性肾病综合征患者血清中呈高表达,sPD-1、sPD-L1检测对肾病综合征预后评估有潜在价值。展开更多
文摘BACKGROUND Necrotising enterocolitis(NEC)is a critical gastrointestinal emergency affecting premature and low-birth-weight neonates.Serum amyloid A(SAA),procalcitonin(PCT),and high-mobility group box 1(HMGB1)have emerged as potential biomarkers for NEC due to their roles in inflammatory response,tissue damage,and immune regulation.AIM To evaluate the diagnostic value of SAA,PCT,and HMGB1 in the context of NEC in newborns.METHODS The study retrospectively analysed the clinical data of 48 newborns diagnosed with NEC and 50 healthy newborns admitted to the hospital.Clinical,radiological,and laboratory findings,including serum SAA,PCT,and HMGB1 Levels,were collected,and specific detection methods were used.The diagnostic value of the biomarkers was evaluated through statistical analysis,which was performed using chi-square test,t-test,correlation analysis,and receiver operating characteristic(ROC)analysis.RESULTS The study demonstrated significantly elevated levels of serum SAA,PCT,and HMGB1 Levels in newborns diagnosed with NEC compared with healthy controls.The correlation analysis indicated strong positive correlations among serum SAA,PCT,and HMGB1 Levels and the presence of NEC.ROC analysis revealed promising sensitivity and specificity for serum SAA,PCT,and HMGB1 Levels as potential diagnostic markers.The combined model of the three biomarkers demonstrating an extremely high area under the curve(0.908).CONCLUSION The diagnostic value of serum SAA,PCT,and HMGB1 Levels in NEC was highlighted.These biomarkers potentially improve the early detection,risk stratification,and clinical management of critical conditions.The findings suggest that these biomarkers may aid in timely intervention and the enhancement of outcomes for neonates affected by NEC.
基金supported by the National Natural Science Foundation of China,No.82001155(to LL)the Natural Science Foundation of Zhejiang Province,No.LY23H090004(to LL)+5 种基金the Natural Science Foundation of Ningbo,No.2023J068(to LL)the Fundamental Research Funds for the Provincial Universities of Zhejiang Province,No.SJLY2023008(to LL)the College Students'Scientific and Technological Innovation Project(Xin Miao Talent Plan)of Zhejiang Province,No.2022R405A045(to CC)the Student ResearchInnovation Program(SRIP)of Ningbo University,Nos.20235RIP1919(to CZ),2023SRIP1938(to YZ)the K.C.Wong Magna Fund in Ningbo University。
文摘A reduction in adult neurogenesis is associated with behavioral abnormalities in patients with Alzheimer's disease.Consequently,enhancing adult neurogenesis represents a promising therapeutic approach for mitigating disease symptoms and progression.Nonetheless,nonpharmacological interventions aimed at inducing adult neurogenesis are currently limited.Although individual non-pharmacological interventions,such as aerobic exercise,acousto-optic stimulation,and olfactory stimulation,have shown limited capacity to improve neurogenesis and cognitive function in patients with Alzheimer's disease,the therapeutic effect of a strategy that combines these interventions has not been fully explored.In this study,we observed an age-dependent decrease in adult neurogenesis and a concurrent increase in amyloid-beta accumulation in the hippocampus of amyloid precursor protein/presenilin 1 mice aged 2-8 months.Amyloid deposition became evident at 4 months,while neurogenesis declined by 6 months,further deteriorating as the disease progressed.However,following a 4-week multifactor stimulation protocol,which encompassed treadmill running(46 min/d,10 m/min,6 days per week),40 Hz acousto-optic stimulation(1 hour/day,6 days/week),and olfactory stimulation(1 hour/day,6 days/week),we found a significant increase in the number of newborn cells(5'-bromo-2'-deoxyuridine-positive cells),immature neurons(doublecortin-positive cells),newborn immature neurons(5'-bromo-2'-deoxyuridine-positive/doublecortin-positive cells),and newborn astrocytes(5'-bromo-2'-deoxyuridine-positive/glial fibrillary acidic protein-positive cells).Additionally,the amyloid-beta load in the hippocampus decreased.These findings suggest that multifactor stimulation can enhance adult hippocampal neurogenesis and mitigate amyloid-beta neuropathology in amyloid precursor protein/presenilin 1 mice.Furthermore,cognitive abilities were improved,and depressive symptoms were alleviated in amyloid precursor protein/presenilin 1 mice following multifactor stimulation,as evidenced by Morris water maze,novel object recognition,forced swimming test,and tail suspension test results.Notably,the efficacy of multifactor stimulation in consolidating immature neurons persisted for at least 2weeks after treatment cessation.At the molecular level,multifactor stimulation upregulated the expression of neuron-related proteins(NeuN,doublecortin,postsynaptic density protein-95,and synaptophysin),anti-apoptosis-related proteins(Bcl-2 and PARP),and an autophagyassociated protein(LC3B),while decreasing the expression of apoptosis-related proteins(BAX and caspase-9),in the hippocampus of amyloid precursor protein/presenilin 1 mice.These observations might be attributable to both the brain-derived neurotrophic factor-mediated signaling pathway and antioxidant pathways.Furthermore,serum metabolomics analysis indicated that multifactor stimulation regulated differentially expressed metabolites associated with cell apoptosis,oxidative damage,and cognition.Collectively,these findings suggest that multifactor stimulation is a novel non-invasive approach for the prevention and treatment of Alzheimer's disease.
文摘Objective: To investigate the expression level and correlation of soluble programmed death factor (sPD-L1) and vascular endothelial growth factor (VEGF) in serum of patients with acute myeloid leukemia.Methods: From January 2016 to October 2017, 36 cases with acute myeloid leukemia were selected as AML group. After 1-2 courses of standard chemotherapy, 27 cases with complete remission were selected as complete remission group, 9 cases without improvement as a group. In the same period, 20 healthy patients were selected as the healthy control group. The expression levels of sPD-L1 and VEGF were detected by enzyme-linked immunoassay (ELISA).Results: The expression of sPD-L1 and VEGF in serum of the initial and unrelieved AML patients was significantly higher than that in the healthy control group, and the difference was statistically significant. After 1-2 courses of standard chemotherapy, the expression of sPD-L1 and VEGF in the serum of patients with complete remission was significantly lower than that in the initial treatment group, and the difference was statistically significant. There was no statistically significant difference between the total relief group and the control group. There is a significant positive correlation between the two expressions. Conclusions: The high expression of sPD-L1and VEGF in serum may be related to the occurrence, development and prognosis of AML. Therefore, the joint detection of sPD-L1 and VEGF is of some significance to the early diagnosis, monitoring efficacy and prognosis of AML patients.
文摘目的探讨可溶性程序性死亡蛋白1(soluble programmed death protein 1,sPD-1)、可溶性程序性死亡蛋白配体1(soluble programmed death protein ligand 1,sPD-L1)在原发性肾病综合征(primary nephrotic syndrome,PNS)患者中的表达及其临床意义。方法选择2016年4月至2019年4月我院收治的PNS患者87例作为病例组,同期在我院健康体检者87例作为对照组,比较两组血清sPD-1、sPD-L1水平,比较不同病理类型PNS患者血清sPD-1、sPD-L1水平,比较不同转归情况PNS患者血清sPD-1、sPD-L1水平,采用P earson相关分析PNS患者血清sPD-1、sPD-L1水平的相关性,采用多因素Logistic回归分析PNS发生的危险因素。结果病例组治疗前血清sPD-1、sPD-L1水平明显高于对照组,差异具有统计学意义(P<0.05);3种病理类型的PNS患者治疗前血清sPD-1、sPD-L1水平差异具有统计学意义(P<0.05);3种病理类型的PNS患者治疗后血清sPD-1、sPD-L1水平差异具有统计学意义(P<0.05);3种病理类型的PNS患者治疗前、后血清sPD-1、sPD-L1水平差异均具有统计学意义(P<0.05);不同转归情况PNS患者治疗后血清sPD-1、sPD-L1水平差异具有统计学意义(P<0.05);PNS患者血清sPD-1、sPD-L1水平呈正相关(P<0.05);sPD-1、sPD-L1是PNS的影响因素(P<0.05)。结论sPD-1、sPD-L1在原发性肾病综合征患者血清中呈高表达,sPD-1、sPD-L1检测对肾病综合征预后评估有潜在价值。
