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The Proof of the Sufficient and Necessary Conditions which Make the Hausdorff Measure of the Generalized Non-uniform Cantor Set'exist
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作者 ZENG Chao-yi 《Chinese Quarterly Journal of Mathematics》 CSCD 北大核心 2006年第2期293-296,共4页
纸基于重复(1 ) 在获得接替概括不一致的领唱者集合的一个班:Si (x)=aix + 双性人, x [0,1 ] , i = 1,2,, m 0ai1, i=1, 2,, m;双性人 + ai0, i = 1,2,, m-1, b1=0 并且是 + bm = 1。提供它的存在 Hausdorff 措施的足够... 纸基于重复(1 ) 在获得接替概括不一致的领唱者集合的一个班:Si (x)=aix + 双性人, x [0,1 ] , i = 1,2,, m 0ai1, i=1, 2,, m;双性人 + ai0, i = 1,2,, m-1, b1=0 并且是 + bm = 1。提供它的存在 Hausdorff 措施的足够、必要的条件。 展开更多
关键词 自相似设置 Hausdorff量纲 Hausdorff测量 必然条件
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SETD5介导AKT1磷酸化调控结肠癌细胞的迁移和5-FU敏感性
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作者 黄开禹 史建国 程勇 《中国肿瘤生物治疗杂志》 CAS CSCD 北大核心 2024年第6期586-591,共6页
目的:探讨含SET结构域蛋白5(SETD5)对结肠癌细胞增殖、迁移和对5-氟尿嘧啶(5-FU)药物敏感性的影响及机制。方法:常规培养结肠癌细胞,用Lipofectamine 2000将siSETD5-NC、si-SETD5-1~3质粒转染至HT-29细胞中,将其分为对照组(未处理)、si-... 目的:探讨含SET结构域蛋白5(SETD5)对结肠癌细胞增殖、迁移和对5-氟尿嘧啶(5-FU)药物敏感性的影响及机制。方法:常规培养结肠癌细胞,用Lipofectamine 2000将siSETD5-NC、si-SETD5-1~3质粒转染至HT-29细胞中,将其分为对照组(未处理)、si-SETD5-NC组、si-SETD5组和si-SETD5+SC79组,si-SETD5+SC79组HT-29细胞转染质粒的同时用10µmol/L SC79处理。qPCR法检测NCM460、HT-29和LoVo细胞中SETD5 mRNA表达,流式细胞术、细胞划痕法、WB法和CCK-8法分别检测各组HT-29细胞的凋亡情况、迁移能力、相关蛋白的表达,以及对5-FU的敏感性。结果:SETD5 mRNA在HT-29、LoVo细胞中均呈高表达(均P<0.01)。在HT-29细胞中成功地敲减了SETD5 mRNA(P<0.01)。敲减SETD5 mRNA可明显抑制HT-29细胞的增殖活性(P<0.01)、迁移能力(P<0.01)、相关蛋白(SETD5、p-PI3K、p-AKT1、p-mTOR蛋白)的表达(均P<0.01)、促进细胞凋亡(P<0.01),且提高其对5-FU的敏感性(P<0.01),这些作用均可被AKT激活剂SC79部分阻挡(P<0.05或P<0.01)。结论:SETD5在HT-29、LoVo细胞中高表达,SETD5通过PI3K/AKT1通路促进结肠癌HT-29细胞的增殖、迁移,且降低其对5-FU的敏感性,SETD5是结肠癌临床诊断、治疗的潜在靶点。 展开更多
关键词 结肠癌 含有set结构域蛋白5 蛋白激酶B磷酸化 增殖 迁移 5-氟尿嘧啶 耐药
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基于梯度增量level-set方法的复杂物体入水砰击数值模拟
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作者 石伏龙 李洪鑫 +2 位作者 辛建建 袁琪 季孟洁 《船舶力学》 EI CSCD 北大核心 2024年第6期877-886,共10页
复杂结构物自由表面砰击问题,如空投鱼雷、水空无人航行器潜水和高速船出入水,一直是海洋工程领域的研究热点。本文采用梯度增量level-set和虚拟网格法数值模拟复杂结构物的入水砰击,采用时间半隐式有限差分法求解不可压缩Navier-Stoke... 复杂结构物自由表面砰击问题,如空投鱼雷、水空无人航行器潜水和高速船出入水,一直是海洋工程领域的研究热点。本文采用梯度增量level-set和虚拟网格法数值模拟复杂结构物的入水砰击,采用时间半隐式有限差分法求解不可压缩Navier-Stokes方程,虚拟网格法可重构复杂结构物界面插值模块并施加无滑移边界条件,梯度增量level-set法可捕捉非线性自由表面翻卷、射流等物理现象。基于以上方法模拟二维圆柱的常速入水砰击,并与试验和数值结果对比,验证本文数值方法的正确性。最后,数值模拟二维船型剖面和飞机剖面的入水砰击,分析了冲击压力、运动响应、压力分布和自由表面运动随入水角度的变化规律。同时,通过模拟可观察到复杂结构浸入水中后的流体分离、射流和小角度空气腔等典型砰击物理现象。 展开更多
关键词 虚拟网格 浸入边界法 levelset 砰击 复杂结构
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Arterial Hypertension-Related Factors within Custodial Settings of Southern Benin in 2023
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作者 Mongbo Virginie Kpozehouen Alphonse Achille Kpétékousso 《Open Journal of Epidemiology》 2024年第1期157-166,共10页
Introduction: Hypertension, a non-communicable disease, is a major public health threat worldwide, accounting for a high level of morbidity and mortality. Although it has been extensively published among the general p... Introduction: Hypertension, a non-communicable disease, is a major public health threat worldwide, accounting for a high level of morbidity and mortality. Although it has been extensively published among the general population, further research is needed to understand the reality of hypertension within the custodial setting. This study aimed to investigate the factors associated with arterial hypertension in custodial settings in southern Benin in 2023. Methods: This was a cross-sectional, descriptive, analytical study held in prisons in southern Benin from March to April 2023, involving inmates selected by two-stage random sampling. In the first stage, four prisons out of the six in the southern region of Benin were selected by simple random sampling. In the second stage, the prisoners were selected by systematic random sampling, with the sampling frame being the numbered list of eligible prisoners in each prison selected. Data collected by observation and questionnaire survey were analyzed using Stata 11 software. Hypertension was defined as systolic blood pressure ≥ 140 mmHg and diastolic blood pressure ≥ 90 mmHg. Overweight was defined by a body mass index (weight/height<sup>2</sup> (kg/m<sup>2</sup>) ≥ 25. Factors associated with hypertension were identified by multiple logistic regression, at a 5% threshold of significance. Results: Altogether 336 inmates aged 37.55 ± 1.72 years were surveyed. The prevalence of hypertension in custodial settings in southern Benin in 2023 was 31.32% (95% CI [17.06;52.57]). Associated factors were inmate age (ORa = 3.36 95% CI: [1.94;5.85]) and abnormal waist circumference (ORa = 2.61 95% CI [1.27;5.40]). Conclusion: The prevalence of arterial hypertension in prisons of southern Benin (31.32%) is high when compared with the national average (25.9% (22.5-29.3)). The ministries of the Interior and Health need to collaborate to involve inmates in preventive strategies for non-communicable diseases, including hypertension. 展开更多
关键词 Arterial Hypertension Associated Factors INMATES Custodial setting BENIN
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Incidence and Survivability of Acute Lymphocytic Leukemia Patients in the United States: Analysis of SEER Data Set from 2000-2019
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作者 Ishan Ghosh Sudipto Mukherjee 《Journal of Cancer Therapy》 2024年第4期141-163,共23页
The main goal of this research is to assess the impact of race, age at diagnosis, sex, and phenotype on the incidence and survivability of acute lymphocytic leukemia (ALL) among patients in the United States. By takin... The main goal of this research is to assess the impact of race, age at diagnosis, sex, and phenotype on the incidence and survivability of acute lymphocytic leukemia (ALL) among patients in the United States. By taking these factors into account, the study aims to explore how existing cancer registry data can aid in the early detection and effective treatment of ALL in patients. Our hypothesis was that statistically significant correlations exist between race, age at which patients were diagnosed, sex, and phenotype of the ALL patients, and their rate of incidence and survivability data were evaluated using SEER*Stat statistical software from National Cancer Institute. Analysis of the incidence data revealed that a higher prevalence of ALL was among the Caucasian population. The majority of ALL cases (59%) occurred in patients aged between 0 to 19 years at the time of diagnosis, and 56% of the affected individuals were male. The B-cell phenotype was predominantly associated with ALL cases (73%). When analyzing survivability data, it was observed that the 5-year survival rates slightly exceeded the 10-year survival rates for the respective demographics. Survivability rates of African Americans patients were the lowest compared to Caucasian, Asian, Pacific Islanders, Alaskan Native, Native Americans and others. Survivability rates progressively decreased for older patients. Moreover, this study investigated the typical treatment methods applied to ALL patients, mainly comprising chemotherapy, with occasional supplementation of radiation therapy as required. The study demonstrated the considerable efficacy of chemotherapy in enhancing patients’ chances of survival, while those who remained untreated faced a less favorable prognosis from the disease. Although a significant amount of data and information exists, this study can help doctors in the future by diagnosing patients with certain characteristics. It will further assist the health care professionals in screening potential patients and early detection of cases. This could also save the lives of elderly patients who have a higher mortality rate from this disease. 展开更多
关键词 Acute Lymphocytic Leukemia SURVIVABILITY INCIDENCE DEMOGRAPHY SEER Data set
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Flow Rate Measurement of Gravity Infusion Set and Functional Evaluation of Drop Counter: A Pilot Study
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作者 Rina Sakai Shuichi Tanaka +3 位作者 Kaya Murakami Tomomi Mizuhashi Kazuhiro Yoshida Masanobu Ujihira 《Journal of Biomedical Science and Engineering》 2024年第7期129-135,共7页
Developing a novel drop counter by introducing the Internet of Things concept has been vigorously conducted in recent years. Understanding the newly introduced drop counter’s flow rate control accuracy and flow rate ... Developing a novel drop counter by introducing the Internet of Things concept has been vigorously conducted in recent years. Understanding the newly introduced drop counter’s flow rate control accuracy and flow rate count feature is essential for improving safety in infusion management. This study aimed to verify if the new drop counters could secure accurate flow rate and drip count by conducting actual flow rate measurements using gravimetry and functional evaluation. A drop counter was attached to each drip chamber of the infusion set, and an IV drip was conducted at the 100 ml/h flow rate. The weight of discharged physiological saline was measured to plot trumpet curves. Next, three different types of drop counters were evaluated to determine if they maintained drip count accuracy according to the changes in their position angles. The flow rate errors in all conditions indicated trumpet-like curves, exhibiting an overall error range within ±10% in all observation windows. Although every drop counter successfully detected and measured dripping, it was challenging in some counters to detect dripping when the drip chamber was tilted. In comparing adult and pediatric IV sets, the adult IV set was found to be less likely to detect dripping in the angled position. No significant differences in results were confirmed between high and low flow rates, suggesting that the drop count function would not be affected by the flow rate in the ranges of typical infusion practices. Doppler sensors have a wide range of measurements and high sensitivity;the dripping was detected successfully even when the drip chamber was tilted, probably due to the advantages of these sensors. In contrast, miscounts occurred in those equipped with infrared sensors, which could not detect light intensity changes in tilted positions. Understanding the tendencies in flow rate errors in infusion can be valuable information for infusion management. 展开更多
关键词 Gravity Infusion set Drop Counter IV Fluids Flow Rate Trumpet Curves
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浮针结合SET技术治疗腰背肌筋膜炎临床观察
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作者 李玉实 朱春燕 +1 位作者 王红蕾 郑一波 《中国民族民间医药》 2024年第13期108-111,共4页
目的:观察浮针结合SET技术(悬吊运动)治疗腰背肌筋膜炎疗效并探究其作用机理。方法:选取腰背肌筋膜炎患者90例,应用随机数字表法随机分为治疗组和对照组(各45例),治疗组应用浮针结合SET技术进行治疗;对照组单纯采用浮针治疗,于治疗前、... 目的:观察浮针结合SET技术(悬吊运动)治疗腰背肌筋膜炎疗效并探究其作用机理。方法:选取腰背肌筋膜炎患者90例,应用随机数字表法随机分为治疗组和对照组(各45例),治疗组应用浮针结合SET技术进行治疗;对照组单纯采用浮针治疗,于治疗前、后,采用简化McGill量表和功能障碍评价量表(Roland-Morris)对两组进行评定比较,并于1月后回访患者统计复发率。结果:治疗2周后,两组的疼痛及功能障碍评分较治疗前均有改善(P<0.05),且治疗组优于对照组(P<0.05);治疗组和对照组的有效率分别为93.33%和88.89%,治疗组优于对照组;整个研究期间,90例患者均未出现不良反应。结论:浮针结合SET技术可显著改善腰背肌筋膜炎患者的疼痛及功能障碍,疗效优于对照组,并可显著降低本病的复发率。 展开更多
关键词 浮针 set技术 腰背肌筋膜炎 腰痛 肌筋膜触发点
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Watson-Jones Anatomical Approach for Open Reduction and Internal Fixation of Proximal Femoral Fractures without Image Intensifier in a Low-Resource Setting
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作者 Loïc Fonkoue Gaspary Fodjeu +6 位作者 Kennedy Olivier Muluem Olivier Ngongang Theophile Nana Marie Ange Ngo Yamben DésiréAkaba Urich Tambekou Daniel Eone Handy 《Open Journal of Orthopedics》 2024年第4期173-186,共14页
Introduction: Standard procedures for surgical fixation of proximal femoral fractures (PFF) require an image intensifier which in developing countries remains a luxury. We hypothesized that, with a well-codified techn... Introduction: Standard procedures for surgical fixation of proximal femoral fractures (PFF) require an image intensifier which in developing countries remains a luxury. We hypothesized that, with a well-codified technique, the Watson Jones approach (WJA) without image intensifier nor traction table, can allow open reduction and internal fixation (ORIF) of PFF using Dynamic hip screw (DHS), with satisfactory outcome. Patients and methods: Forty one consecutive patients (mean age 59.5 ± 21.6 years, 61% males) who were followed in a Teaching Hospital for PFF treated by ORIF using the WJA and DHS from January 2016 to December 2020 were reassessed. The outcome measures were the quality of the reduction, the positioning of the implants, the tip-apex distance (TAD), the rate and delay of consolidation, the functional results using Postel Merle d’Aubigné (PMA) score, the rate of surgical site infection (SSI) and the overall mortality. Logistic regression was used to determine factors associated with mechanical failure. Results: The mean follow-up period was 33.8 ± 15.0 months. Fracture reduction was good in 31 (75.6%) cases and acceptable in 8(19.5%) cases. Implant position was fair to good in 37 (90.2%) patients. The mean TAD was 26.1 ± 3.9 mm. Three patients developed SSI. Consolidation was achieved in 38 (92.6%) patients. The functional results were good to excellent in 80.5% of patients. The overall mortality rate was 7.3%. There were an association between mechanical failure and osteoporosis (p = 0.04), fracture reduction (p = 0.003), and TAD (p = 0.025). In multivariate logistic regression, no independent factors were predictive of mechanical failure. Conclusion: This study shows that ORIF using DHS for PFF via the Watson-Jones approach without an image intensifier can give satisfactory anatomical and functional outcomes in low-resource settings. It provides and validates a reliable and reproducible technique that deserves to be diffused to surgeons in austere areas over the world. 展开更多
关键词 Proximal Femoral Fracture Watson-Jones Approach Dynamic Hip Screw Low Resource setting
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Multi-Granularity Neighborhood Fuzzy Rough Set Model on Two Universes
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作者 Ju Wang Xinghu Ai Li Fu 《Journal of Intelligent Learning Systems and Applications》 2024年第2期91-106,共16页
The two universes multi-granularity fuzzy rough set model is an effective tool for handling uncertainty problems between two domains with the help of binary fuzzy relations. This article applies the idea of neighborho... The two universes multi-granularity fuzzy rough set model is an effective tool for handling uncertainty problems between two domains with the help of binary fuzzy relations. This article applies the idea of neighborhood rough sets to two universes multi-granularity fuzzy rough sets, and discusses the two-universes multi-granularity neighborhood fuzzy rough set model. Firstly, the upper and lower approximation operators are defined in the two universes multi-granularity neighborhood fuzzy rough set model. Secondly, the properties of the upper and lower approximation operators are discussed. Finally, the properties of the two universes multi-granularity neighborhood fuzzy rough set model are verified through case studies. 展开更多
关键词 Fuzzy set Two Universes Multi-Granularity Rough set Multi-Granularity Neighborhood Fuzzy Rough set
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术前血清V-set和免疫球蛋白结构域4以及长链非编码核糖核酸SBF2反义RNA1与肾结石患者经皮肾镜取石术后急性肾损伤的关系
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作者 姚俊波 贾波 +2 位作者 刘加元 邹一鸣 邓思文 《实用临床医药杂志》 CAS 2024年第10期29-34,共6页
目的探讨术前血清V-set和免疫球蛋白结构域4(VSIG4)以及长链非编码核糖核酸(LncRNA)SBF2反义RNA1(SBF2-AS1)与肾结石患者经皮肾镜取石术后急性肾损伤(AKI)的关系。方法选择2020年1月—2022年12月本院收治的109例肾结石患者为研究对象。... 目的探讨术前血清V-set和免疫球蛋白结构域4(VSIG4)以及长链非编码核糖核酸(LncRNA)SBF2反义RNA1(SBF2-AS1)与肾结石患者经皮肾镜取石术后急性肾损伤(AKI)的关系。方法选择2020年1月—2022年12月本院收治的109例肾结石患者为研究对象。术前检测患者血清VSIG4水平以及LncRNA SBF2-AS1表达,术后记录AKI发生情况。采用多因素Logistic回归模型分析肾结石患者经皮肾镜取石术后发生AKI的影响因素;采用受试者工作特征(ROC)曲线分析VSIG4、LncRNA SBF2-AS1预测肾结石患者经皮肾镜取石术后发生AKI的价值。结果本研究中,术后发生AKI者16例。AKI组血清VSIG4水平低于非AKI组,LncRNA SBF2-AS1表达高于非AKI组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析显示,术前高尿酸水平、术前高LncRNA SBF2-AS1表达、较长的手术时间、术中低血压是肾结石患者经皮肾镜取石术后发生AKI的危险因素(P<0.05),术前高VSIG4水平是保护因素(P<0.05)。术前血清VSIG4、LncRNA SBF2-AS1水平预测肾结石患者经皮肾镜术后发生AKI的曲线下面积分别为0.854、0.705,二者联合预测的曲线下面积为0.948,大于各指标单独预测(Z=1.995、2.958,P<0.05)。结论肾结石患者术前血清VSIG4水平降低、LncRNA SBF2-AS1表达增高与经皮肾镜取石术后AKI的发生有关,联合检测术前VSIG4、LncRNA SBF2-AS1可预测术后AKI的发生风险。 展开更多
关键词 肾结石 经皮肾镜取石术 急性肾损伤 V-set和免疫球蛋白结构域4 长链非编码核糖核酸 SBF2反义RNA 1
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Approximation Maintenance When Adding a Conditional Value in Set-Valued Ordered Decision Systems
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作者 Xiaoyu Wang Yuebin Su 《Open Journal of Applied Sciences》 2024年第2期411-424,共14页
The integration of set-valued ordered rough set models and incremental learning signify a progressive advancement of conventional rough set theory, with the objective of tackling the heterogeneity and ongoing transfor... The integration of set-valued ordered rough set models and incremental learning signify a progressive advancement of conventional rough set theory, with the objective of tackling the heterogeneity and ongoing transformations in information systems. In set-valued ordered decision systems, when changes occur in the attribute value domain, such as adding conditional values, it may result in changes in the preference relation between objects, indirectly leading to changes in approximations. In this paper, we effectively addressed the issue of updating approximations that arose from adding conditional values in set-valued ordered decision systems. Firstly, we classified the research objects into two categories: objects with changes in conditional values and objects without changes, and then conducted theoretical studies on updating approximations for these two categories, presenting approximation update theories for adding conditional values. Subsequently, we presented incremental algorithms corresponding to approximation update theories. We demonstrated the feasibility of the proposed incremental update method with numerical examples and showed that our incremental algorithm outperformed the static algorithm. Ultimately, by comparing experimental results on different datasets, it is evident that the incremental algorithm efficiently reduced processing time. In conclusion, this study offered a promising strategy to address the challenges of set-valued ordered decision systems in dynamic environments. 展开更多
关键词 Information Systems Rough set Attribute Value Incremental Method
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Phase-Based Optical Flow Method with Optimized Parameter Settings for Enhancing Displacement Measurement Adaptability
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作者 Zhaoxin Peng Menglian Liu +2 位作者 Zhiliang Wang Wei Liu Xian Wang 《Open Journal of Applied Sciences》 2024年第5期1165-1184,共20页
To enhance the applicability and measurement accuracy of phase-based optical flow method using complex steerable pyramids in structural displacement measurement engineering applications, an improved method of optimizi... To enhance the applicability and measurement accuracy of phase-based optical flow method using complex steerable pyramids in structural displacement measurement engineering applications, an improved method of optimizing parameter settings is proposed. The optimized parameters include the best measurement points of the Region of Interest (ROI) and the levels of pyramid filters. Additionally, to address the issue of updating reference frames in practical applications due to the difficulty in estimating the maximum effective measurement value, a mechanism for dynamically updating reference frames is introduced. Experimental results demonstrate that compared to representative image gradient-based displacement measurement methods, the proposed method exhibits higher measurement accuracy in engineering applications. This provides reliable data support for structural damage identification research based on vibration signals and is expected to broaden the engineering application prospects for structural health monitoring. 展开更多
关键词 Displacement Measurement Phase-Based Optical Flow Optimized Parameter setting
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Pseudo-Semi-Overlap Functions-Based Fuzzy Rough Sets Applied to Image Edge Extraction
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作者 Ran Yin Minge Chen +2 位作者 Yu Liu Yafei Zhao Jianwei Li 《Journal of Applied Mathematics and Physics》 2024年第7期2347-2366,共20页
As an extension of overlap functions, pseudo-semi-overlap functions are a crucial class of aggregation functions. Therefore, (I, PSO)-fuzzy rough sets are introduced, utilizing pseudo-semi-overlap functions, and furth... As an extension of overlap functions, pseudo-semi-overlap functions are a crucial class of aggregation functions. Therefore, (I, PSO)-fuzzy rough sets are introduced, utilizing pseudo-semi-overlap functions, and further extended for applications in image edge extraction. Firstly, a new clustering function, the pseudo-semi-overlap function, is introduced by eliminating the symmetry and right continuity present in the overlap function. The relaxed nature of this function enhances its applicability in image edge extraction. Secondly, the definitions of (I, PSO)-fuzzy rough sets are provided, using (I, PSO)-fuzzy rough sets, a pair of new fuzzy mathematical morphological operators (IPSOFMM operators) is proposed. Finally, by combining the fuzzy C-means algorithm and IPSOFMM operators, a novel image edge extraction algorithm (FCM-IPSO algorithm) is proposed and implemented. Compared to existing algorithms, the FCM-IPSO algorithm exhibits more image edges and a 73.81% decrease in the noise introduction rate. The outstanding performance of (I, PSO)-fuzzy rough sets in image edge extraction demonstrates their practical application value. 展开更多
关键词 Pseudo-Semi-Overlap Functions Fuzzy Rough set Fuzzy Mathematical Morphology Image Edge Extraction
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On the Proof of the Contradiction of Set Theory
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作者 Yury M. Volin 《Advances in Pure Mathematics》 2024年第3期139-159,共21页
The article is devoted to proving the inconsistency of set theory arising from the existence of strange trees. All steps of the proof rely on common informal set-theoretic reasoning, but they take into account the pro... The article is devoted to proving the inconsistency of set theory arising from the existence of strange trees. All steps of the proof rely on common informal set-theoretic reasoning, but they take into account the prohibitions that were introduced into axiomatic set theories in order to overcome the difficulties encountered by the naive Cantor set theory. Therefore, in fact, the article is about proving the inconsistency of existing axiomatic set theories, in particular, the ZFC theory. 展开更多
关键词 set Theory Inconsistency TREE Strange Tree Through Way Almost through Way ISOMORPHISM Almost Isomorphism Isomorphism Tree Place Plane Superposition of Trees on the Place Plane Disposition of Trees on the Place Plane
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SETD1A与KDM4A在非小细胞肺癌中的表达及临床价值 被引量:2
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作者 朱小慧 李亚云 +2 位作者 张海燕 刘海涛 于志娟 《疑难病杂志》 CAS 2023年第9期945-950,共6页
目的研究非小细胞肺癌(NSCLC)组织中组蛋白赖氨酸甲基转移酶SET结构域包含1A(SETD1A)和赖氨酸去甲基酶4A(KDM4A)表达及与临床病理特征及预后的关系。方法选取2017年1月—2020年1月南通市肿瘤医院综合内科收治NSCLC患者116例为研究对象... 目的研究非小细胞肺癌(NSCLC)组织中组蛋白赖氨酸甲基转移酶SET结构域包含1A(SETD1A)和赖氨酸去甲基酶4A(KDM4A)表达及与临床病理特征及预后的关系。方法选取2017年1月—2020年1月南通市肿瘤医院综合内科收治NSCLC患者116例为研究对象。采用免疫组织化学法检测NSCLC组织中SETD1A、KDM4A表达,二者相关性分析采用Spearman秩相关分析。Kaplan-Meier生存分析SETD1A、KDM4A表达对NSCLC患者生存预后的影响。Cox回归分析影响NSCLC患者预后因素。结果NSCLC癌组织中SETD1A棕黄色阳性染色主要位于细胞核,KDM4A阳性染色主要位于细胞浆和细胞膜。NSCLC癌组织SETD1A阳性率为61.21%(71/116),高于癌旁组织的6.90%(8/116),差异有统计学意义(χ^(2)=76.546,P<0.001)。NSCLC癌组织中KDM4A阳性率为65.52%(76/116),高于癌旁组织的8.62%(10/116),差异有统计学意义(χ^(2)=80.487,P<0.001)。NSCLC癌组织中SETD1A与KDM4A表达呈显著正相关(rs=0.722,P<0.001)。TNM分期Ⅲ期、低分化程度、伴淋巴结转移患者在NSCLC癌组织中SETD1A、KDM4A阳性率显著高于TNM分期Ⅰ~Ⅱ期、高中分化程度、无淋巴结转移者(SETD1A:χ^(2)/P=15.808/<0.001,7.108/0.008,17.136/<0.001;KDM4A:χ^(2)/P=9.050/0.003,5.480/0.019,10.208/0.001)。SETD1A阳性组和阴性组患者3年总体生存率分别为49.28%(34/69)、78.72%(37/47)。SETD1A阳性组患者3年累积生存率明显低于阴性组患者(χ^(2)=12.984,P<0.001);KDM4A阳性组和阴性组3年总体生存率分别为48.65%(36/74)、83.33%(35/42),KDM4A阳性组患者3年累积生存率明显低于阴性组患者(χ^(2)=17.175,P<0.001)。TNM分期Ⅲ期、合并淋巴结转移、SETD1A阳性、KDM4A阳性是影响NSCLC患者不良预后的独立危险因素[OR(95%CI)=1.610(1.221~2.122)、1.904(1.307~2.774)、1.835(1.228~2.742)、1.744(1.245~2.443)]。结论NSCLC癌组织中SETD1A、KDM4A表达升高,两者表达与TNM分期、分化程度及淋巴结转移有关,检测NSCLC组织中SETD1A、KDM4A表达有助于评估NSCLC患者临床预后。 展开更多
关键词 非小细胞肺癌 组蛋白赖氨酸甲基转移酶set结构域包含1A 赖氨酸去甲基酶4A 预后
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甲基转移酶SETDB1和SOX7甲基化在口腔癌中的相关性研究 被引量:2
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作者 郝妍 白相宇 +1 位作者 霍峰 陈喜波 《河北医药》 CAS 2023年第16期2435-2439,共5页
目的检测口腔癌中SETDB1表达与SOX7甲基化水平,分析二者相关性及临床意义。方法收集口腔癌和癌旁组织,焦磷酸测序法检测SOX7基因甲基化水平,RT-qPCR检测SOX7和SETDB1 mRNA的表达,免疫组化及Western blot检测蛋白表达。统计分析SOX7甲基... 目的检测口腔癌中SETDB1表达与SOX7甲基化水平,分析二者相关性及临床意义。方法收集口腔癌和癌旁组织,焦磷酸测序法检测SOX7基因甲基化水平,RT-qPCR检测SOX7和SETDB1 mRNA的表达,免疫组化及Western blot检测蛋白表达。统计分析SOX7甲基化水平与SETDB1相关性及其与临床病例特征的相关性。构建SETDB1下调siRNA转染口腔癌KB细胞,设siRNA-SETDB1为实验组(si-SET)、siRNA空载体为阴性对照组(si-N)和未转染KB细胞为空白对照组(NC)。检测3组SETDB1 mRNA和蛋白水平及SOX7甲基化。结果口腔癌组织SOX7基因甲基化率高于癌旁组织,SOX7 mRNA相对表达量低于癌旁组织,差异均有统计学意义(P<0.05)。癌组织中SETDB1 mRNA相对表达量高于癌旁组织(P<0.05);免疫组化结果显示癌组织SOX7蛋白表达阳性率SETDB1蛋白表达阳性率高于癌旁组织,差异均有统计学意义(P<0.05)。Western Blot结果显示SETDB1在癌组织中表达高于癌旁组织,SOX7在癌组织中表达低于癌旁组织,差异均有统计学意义(P<0.05);口腔癌组织中SOX7甲基化和SETDB1具有相关性(r=0.324,P<0.05);SOX7甲基化和SETDB1与肿瘤TNM分期、组织分化和淋巴结转移密切相关(P<0.05)。体外实验显示,RNA干扰SETDB1后si-RNA组中SETDB1 mRNA和蛋白表达量最低(P<0.05),SOX7基因甲基化率显著下降(P<0.05)。结论在口腔癌病变过程中SETDB1与SOX7基因甲基化具有相关性,SETDB1可能是催化SOX7发生甲基化修饰的重要甲基化转移酶,在口腔早期病变诊治中具潜在价值。 展开更多
关键词 甲基转移酶 setDB1 SOX7 甲基化 口腔癌
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组蛋白甲基化转移酶SETD4对鼻咽癌细胞增殖和迁移的影响 被引量:1
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作者 封慕茵 郑阿秀 +4 位作者 白建荣 曾玉梅 罗泊涛 申志华 揭伟 《中国病理生理杂志》 CAS CSCD 北大核心 2023年第2期259-268,共10页
目的:明确组蛋白甲基化转移酶含SET结构域蛋白4 (SET-domain-containing protein 4, SETD4)在临床鼻咽癌(nasopharyngeal carcinoma, NPC)组织中的表达,分析SETD4对NPC细胞增殖和迁移的影响。方法:采用免疫组织化学法检测86例临床NPC标... 目的:明确组蛋白甲基化转移酶含SET结构域蛋白4 (SET-domain-containing protein 4, SETD4)在临床鼻咽癌(nasopharyngeal carcinoma, NPC)组织中的表达,分析SETD4对NPC细胞增殖和迁移的影响。方法:采用免疫组织化学法检测86例临床NPC标本与对照组30例鼻咽黏膜慢性炎症(nasopharyngeal chronic inflammation,NPI)组织中SETD4蛋白的表达;基于CRISPR/Cas9技术敲除CNE2细胞中SETD4基因,DNA测序结合半定量RTPCR进行鉴定;以SETD4敲除前后的CNE2细胞为研究对象,于倒置显微镜下观察细胞形态,采用CCK-8实验分析细胞增殖活性、Transwell实验观察细胞迁移能力变化、Western blot检测增殖细胞核抗原(proliferating cell nuclear antigen, PCNA)、细胞周期相关蛋白、上皮-间充质转化标志物和组蛋白赖氨酸甲基化的变化,并通过生物信息学富集SETD4相关联的功能和信号通路。结果:SETD4蛋白主要定位于NPC细胞核中,NPC组织中SETD4阳性表达率显著低于NPI对照组(P<0.01)。基于CRISPR/Cas9技术成功敲除了CNE2细胞的SETD4基因,获得基因敲除的纯合子细胞。与野生型(wild-type, WT)细胞株相比,SETD4敲除(knockout, KO)细胞株呈现更明显的梭形和多角形,增殖活性和迁移能力均显著增加(P<0.01),E-cadherin和p21蛋白表达显著下调(P<0.05),而cyclin D1、cyclin E1、Ncadherin和vimentin蛋白表达则显著上调(P<0.05)。此外,与WT组相比,SETD4KO组细胞中H3K4me2、H3K4me3、H3K9me1、H3K27me3、H3K79me2和H4K20me2水平显著降低(P<0.05)。基因集富集结果显示,SETD4与细胞周期的功能和信号通路相关。结论:临床NPC组织中SETD4蛋白的表达减弱或缺失;SETD4表达减弱通过上调细胞周期相关蛋白促进NPC细胞增殖,通过诱导上皮-间充质转化而促进细胞迁移;SETD4影响NPC细胞中H3K4、H3K9、H3K27、H3K79和H4K20多个位点的甲基化。 展开更多
关键词 鼻咽癌 组蛋白甲基转移酶 set结构域蛋白4 细胞增殖 细胞迁移 基因集富集
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基于SET因素分析法的学龄前儿童智能机器人设计研究 被引量:3
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作者 赵宏月 冯乙 《工业设计》 2023年第7期64-67,共4页
为了获得较为科学合理的学龄前儿童智能机器人设计方案,提高用户对儿童智能机器人的满意度,文章首先通过调研分析了儿童智能机器人设计的现状及存在问题。其次归纳学龄前儿童的认知特点和SET因素,对儿童智能机器人的目标用户需求进行拆... 为了获得较为科学合理的学龄前儿童智能机器人设计方案,提高用户对儿童智能机器人的满意度,文章首先通过调研分析了儿童智能机器人设计的现状及存在问题。其次归纳学龄前儿童的认知特点和SET因素,对儿童智能机器人的目标用户需求进行拆解和分析。最后归纳总结出针对学龄前儿童智能机器人的产品机会,提出具有可行性的且易于学龄前儿童使用的儿童智能机器人设计思路。以期为学龄前儿童智能机器人设计提供参考,增强设计方案的可行性与有效性。 展开更多
关键词 set因素分析 认知特点 学龄前儿童 智能机器人设计
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基于SET与KJ法的盲人出行辅助产品设计研究 被引量:1
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作者 林润涛 赵利权 周思洁 《工业设计》 2023年第7期72-75,共4页
SET因素分析法与KJ法是产品设计中常用的方法,文章将二者融合并应用于盲人出行辅助产品的设计中。首先通过SET因素分析法发现产品机会缺口,并对用户需求进行调研;其次,整合用户需求痛点并构思初步设计方针,理清用户需求层级与群组关系,... SET因素分析法与KJ法是产品设计中常用的方法,文章将二者融合并应用于盲人出行辅助产品的设计中。首先通过SET因素分析法发现产品机会缺口,并对用户需求进行调研;其次,整合用户需求痛点并构思初步设计方针,理清用户需求层级与群组关系,为头脑风暴提供聚焦点;再次,运用KJ法对头脑风暴产物进行关联性聚类,明确设计原则,制定设计策略,从而选定设计方案并输出产品;最后,运用Rhino建模与KeyShot渲染软件呈现产品效果。融合SET与KJ法的设计流程能够深入挖掘用户需求,提升产品设计流程的系统性与准确性。 展开更多
关键词 产品设计 set KJ法 盲人 出行辅助产品
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甲状腺乳头状癌组织中SETDB2、Gal-3、PLEKHS1的表达情况及其对患者预后的预测价值
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作者 桑妙玉 杨小洁 +4 位作者 王志慧 张超 刘晓霞 周玲玲 白宝琴 《广西医学》 CAS 2023年第21期2545-2550,共6页
目的分析SET结构域分叉组蛋白赖氨酸甲基转移酶2(SETDB2)、半乳糖凝集素3(Gal-3)、pleckstrin同源结构域S1(PLEKHS1)在甲状腺乳头状癌组织中的表达情况,以及三者对患者预后的预测价值。方法(1)选择118例甲状腺乳头状癌患者作为研究组,4... 目的分析SET结构域分叉组蛋白赖氨酸甲基转移酶2(SETDB2)、半乳糖凝集素3(Gal-3)、pleckstrin同源结构域S1(PLEKHS1)在甲状腺乳头状癌组织中的表达情况,以及三者对患者预后的预测价值。方法(1)选择118例甲状腺乳头状癌患者作为研究组,45例甲状腺良性结节患者作为对照组。采用免疫组化法检测甲状腺乳头状癌组织和甲状腺良性结节组织中SETDB2、Gal-3、PLEKHS1蛋白表达情况。比较研究组与对照组之间,以及不同特征甲状腺乳头状癌患者之间病灶组织中SETDB2、Gal-3、PLEKHS1蛋白的阳性表达率。(2)随访1年,记录甲状腺乳头状癌患者的生存情况。分析SETDB2、Gal-3、PLEKHS1蛋白阳性表达和阴性表达患者的生存率;比较预后良好和预后不良患者SETDB2、Gal-3、PLEKHS1蛋白的阳性表达率。采用受试者工作特征(ROC)曲线分析SETDB2、Gal-3、PLEKHS1对甲状腺乳头状癌患者预后的预测价值。结果(1)与对照组相比,研究组病灶组织中SETDB2蛋白阳性表达率较低,Gal-3、PLEKHS1蛋白阳性表达率较高(P<0.05)。在甲状腺乳头状癌患者中,Ⅲ~Ⅳ期、浸润深度≥1/2、组织中低分化、淋巴结转移、肿瘤直径>1 cm的患者病灶组织中SETDB2蛋白阳性表达率更低,Gal-3、PLEKHS1蛋白阳性表达率更高(P<0.05)。(2)在甲状腺乳头状癌患者中,SETDB2蛋白阳性表达患者生存率高于SETDB2蛋白阴性表达患者,Gal-3、PLEKHS1蛋白阳性表达患者生存率低于Gal-3、PLEKHS1蛋白阴性表达患者(P<0.05);预后良好患者的SETDB2蛋白阳性表达率高于预后不良患者,Gal-3、PLEKHS1蛋白阳性表达率低于预后不良患者(P<0.05)。ROC曲线分析结果显示,SETDB2、Gal-3、PLEKHS1蛋白总评分预测甲状腺乳头状癌患者预后不良的曲线下面积分别为0.826、0.841、0.859。结论在甲状腺乳头状癌中,SETDB2呈低表达,而Gal-3、PLEKHS1呈高表达,三者均与患者的病情严重程度相关,且对预后具有一定的预测价值。 展开更多
关键词 甲状腺乳头状癌 set结构域分叉组蛋白赖氨酸甲基转移酶2 半乳糖凝集素3 pleckstrin同源结构域S1 预后 预测价值
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