Objective To observe the effect of meisoindigo onapoptosis and proliferation of JAK2 /V617F heterozygousmutation cell line-SET2 cell line to further explore therole of JAK-STAT pathway in this effect. Methods Cellapop...Objective To observe the effect of meisoindigo onapoptosis and proliferation of JAK2 /V617F heterozygousmutation cell line-SET2 cell line to further explore therole of JAK-STAT pathway in this effect. Methods Cellapoptosis after treated with different concentration of meisoindigo(0,5,and 10 μmol /L) was evaluated by flowcytometry at different time points (24,48,72 h). Cellproliferation with CCK8 test was evaluated at differenttime points (24,48,72,96 h) after administered withdifferent concentration of meisoindigo (0,5,10,and20 μmol /L). After treatment with different concentrationof meisoindigo (0,5,10,and 20 μmol /L),SET2 cellswere collected after 12 h,and then cultured in incom-plete methylcellulose-based medium for clone formation.JAK-STAT signaling pathway and apoptosis related proteinby Western blot test were evaluated 12 h after administeredwith different concentration of meisoindigo(0,5,10,and 20 μmol /L).展开更多
目的:探讨组蛋白赖氨酸甲基转移酶SET和MYND结构域蛋白2(SET and MYND domain-containing protein 2,SMYD2)在宫颈癌细胞糖代谢中的作用,进一步研究其是否与宫颈癌增殖有关。方法:通过基因表达谱交互分析(gene expression profiling int...目的:探讨组蛋白赖氨酸甲基转移酶SET和MYND结构域蛋白2(SET and MYND domain-containing protein 2,SMYD2)在宫颈癌细胞糖代谢中的作用,进一步研究其是否与宫颈癌增殖有关。方法:通过基因表达谱交互分析(gene expression profiling interactive analysis,GEPIA)数据库分析SMYD家族成员在宫颈癌中的表达和预后价值,通过log-rank检验进行生存差异比较。通过SMYD2的shRNA敲低或过表达技术来研究SMYD2在宫颈癌中的糖代谢作用。结果:宫颈癌组织中SMYD2的表达与患者年龄、性别、肿瘤组织分化程度无关,SMYD2的过表达预示总体生存期较差与临床分期相关(F=4.520,P=0.004)。生物信息分析揭示了SMYD2在调控宫颈癌中Warburg效应中发挥调节作用。通过功能缺失实验,证明SMYD2的敲低抑制了宫颈癌细胞的有氧糖酵解,表现为葡萄糖摄取减少,乳酸生成降低和细胞外酸化减弱,而SMYD2过表达则促进子宫颈癌细胞的糖酵解代谢。此外,SMYD2是宫颈癌细胞生长所必需的,并且这种致癌作用主要依赖糖酵解。结论:SMYD2在宫颈癌细胞中过表达并在肿瘤代谢中发挥重要作用,能够通过调节有氧酵解促进宫颈癌细胞增殖。展开更多
Electronic patient data gives many advantages,but also new difficulties.Deadlocks may delay procedures like acquiring patient information.Distributed deadlock resolution solutions introduce uncertainty due to inaccura...Electronic patient data gives many advantages,but also new difficulties.Deadlocks may delay procedures like acquiring patient information.Distributed deadlock resolution solutions introduce uncertainty due to inaccurate transaction properties.Soft computing-based solutions have been developed to solve this challenge.In a single framework,ambiguous,vague,incomplete,and inconsistent transaction attribute information has received minimal attention.The work presented in this paper employed type-2 neutrosophic logic,an extension of type-1 neutrosophic logic,to handle uncertainty in real-time deadlock-resolving systems.The proposed method is structured to reflect multiple types of knowledge and relations among transactions’features that include validation factor degree,slackness degree,degree of deadline-missed transaction based on the degree of membership of truthiness,degree ofmembership of indeterminacy,and degree ofmembership of falsity.Here,the footprint of uncertainty(FOU)for truth,indeterminacy,and falsity represents the level of uncertainty that exists in the value of a grade of membership.We employed a distributed real-time transaction processing simulator(DRTTPS)to conduct the simulations and conducted experiments using the benchmark Pima Indians diabetes dataset(PIDD).As the results showed,there is an increase in detection rate and a large drop in rollback rate when this new strategy is used.The performance of Type-2 neutrosophicbased resolution is better than the Type-1 neutrosophic-based approach on the execution ratio scale.The improvement rate has reached 10%to 20%,depending on the number of arrived transactions.展开更多
For dealing with large static error due to poor immunity of the traditional fuzzy control, a novel interval type-2 fuzzy control system is proposed. By extending the typical membership functions to interval type-2 mem...For dealing with large static error due to poor immunity of the traditional fuzzy control, a novel interval type-2 fuzzy control system is proposed. By extending the typical membership functions to interval type-2 membership functions, the proposed control system can efficiently reduce the uncertain disturbance from real environment without increasing the design complexity. The simulation results on the water tank level control system showed that the proposed method succeeded in better static and dynamic control with stronger robust performance than the traditional fuzzy control method.展开更多
In view of the environment competencies,selecting the optimal green supplier is one of the crucial issues for enterprises,and multi-criteria decision-making(MCDM)methodologies can more easily solve this green supplier...In view of the environment competencies,selecting the optimal green supplier is one of the crucial issues for enterprises,and multi-criteria decision-making(MCDM)methodologies can more easily solve this green supplier selection(GSS)problem.In addition,prioritized aggregation(PA)operator can focus on the prioritization relationship over the criteria,Choquet integral(CI)operator can fully take account of the importance of criteria and the interactions among them,and Bonferroni mean(BM)operator can capture the interrelationships of criteria.However,most existing researches cannot simultaneously consider the interactions,interrelationships and prioritizations over the criteria,which are involved in the GSS process.Moreover,the interval type-2 fuzzy set(IT2FS)is a more effective tool to represent the fuzziness.Therefore,based on the advantages of PA,CI,BM and IT2FS,in this paper,the interval type-2 fuzzy prioritized Choquet normalized weighted BM operators with fuzzy measure and generalized prioritized measure are proposed,and some properties are discussed.Then,a novel MCDM approach for GSS based upon the presented operators is developed,and detailed decision steps are given.Finally,the applicability and practicability of the proposed methodology are demonstrated by its application in the shared-bike GSS and by comparisons with other methods.The advantages of the proposed method are that it can consider interactions,interrelationships and prioritizations over the criteria simultaneously.展开更多
In this paper, interval type-2 fuzzy sets, fuzzy comprehensive evaluation and the fuzzy control rules are synthesized to realize the control of unmanned vehicle in driving state and behavioral decisions. Compared to t...In this paper, interval type-2 fuzzy sets, fuzzy comprehensive evaluation and the fuzzy control rules are synthesized to realize the control of unmanned vehicle in driving state and behavioral decisions. Compared to the type-1 fuzzy set, type-2 fuzzy sets have more advantages in handling the model based on uncertainties, linguistic information because the membership functions are fuzzy sets. Different membership functions are established for each factor when the unmanned vehicle is driving at different speed intervals. In addition, a new evaluation method is developed to analyze unmanned vehicle’s driving state. Finally, a set of dynamic fuzzy rules are sorted out, which can be applied to the unmanned vehicle’s behavioral decision-making and provide a new idea to related research.展开更多
The modelling and formal characterization of spatial vagueness plays an increasingly important role in the imple- mentation of Geographic Information System (GIS). The concepts involved in spatial objects of GIS have ...The modelling and formal characterization of spatial vagueness plays an increasingly important role in the imple- mentation of Geographic Information System (GIS). The concepts involved in spatial objects of GIS have been investigated and acknowledged as being vague and ambiguous. Models and methods which describe and handle fuzzy or vague (rather than crisp or determinate) spatial objects, will be more necessary in GIS. This paper proposes a new method for modelling spatial vagueness based on type-2 fuzzy set, which is distinguished from the traditional type-1 fuzzy methods and more suitable for describing and implementing the vague concepts and objects in GIS.展开更多
In order to measure the uncertain information of a type- 2 intuitionistic fuzzy set （T21FS）, an entropy measure of T21FS is presented by using the constructive principles. The proposed entropy measure is also proved...In order to measure the uncertain information of a type- 2 intuitionistic fuzzy set （T21FS）, an entropy measure of T21FS is presented by using the constructive principles. The proposed entropy measure is also proved to satisfy all of the constructive principles. Further, a novel concept of the type-2 triangular in- tuitionistic trapezoidal fuzzy set （T2TITrFS） is developed, and a geometric interpretation of the T2TITrFS is given to comprehend it completely or correctly in a more intuitive way. To deal with a more general uncertain complex system, the constructive principles of an entropy measure of T2TITrFS are therefore proposed on the basis of the axiomatic definition of the type-2 intuitionisic fuzzy entropy measure. This paper elicits a formula of type-2 triangular intuitionistic trapezoidal fuzzy entropy and verifies that it does sa- tisfy the constructive principles. Two examples are given to show the efficiency of the proposed entropy of T2TITrFS in describing the uncertainty of the type-2 intuitionistic fuzzy information and illustrate its application in type-2 triangular intuitionistic trapezodial fuzzy decision making problems.展开更多
文摘Objective To observe the effect of meisoindigo onapoptosis and proliferation of JAK2 /V617F heterozygousmutation cell line-SET2 cell line to further explore therole of JAK-STAT pathway in this effect. Methods Cellapoptosis after treated with different concentration of meisoindigo(0,5,and 10 μmol /L) was evaluated by flowcytometry at different time points (24,48,72 h). Cellproliferation with CCK8 test was evaluated at differenttime points (24,48,72,96 h) after administered withdifferent concentration of meisoindigo (0,5,10,and20 μmol /L). After treatment with different concentrationof meisoindigo (0,5,10,and 20 μmol /L),SET2 cellswere collected after 12 h,and then cultured in incom-plete methylcellulose-based medium for clone formation.JAK-STAT signaling pathway and apoptosis related proteinby Western blot test were evaluated 12 h after administeredwith different concentration of meisoindigo(0,5,10,and 20 μmol /L).
文摘目的:探讨组蛋白赖氨酸甲基转移酶SET和MYND结构域蛋白2(SET and MYND domain-containing protein 2,SMYD2)在宫颈癌细胞糖代谢中的作用,进一步研究其是否与宫颈癌增殖有关。方法:通过基因表达谱交互分析(gene expression profiling interactive analysis,GEPIA)数据库分析SMYD家族成员在宫颈癌中的表达和预后价值,通过log-rank检验进行生存差异比较。通过SMYD2的shRNA敲低或过表达技术来研究SMYD2在宫颈癌中的糖代谢作用。结果:宫颈癌组织中SMYD2的表达与患者年龄、性别、肿瘤组织分化程度无关,SMYD2的过表达预示总体生存期较差与临床分期相关(F=4.520,P=0.004)。生物信息分析揭示了SMYD2在调控宫颈癌中Warburg效应中发挥调节作用。通过功能缺失实验,证明SMYD2的敲低抑制了宫颈癌细胞的有氧糖酵解,表现为葡萄糖摄取减少,乳酸生成降低和细胞外酸化减弱,而SMYD2过表达则促进子宫颈癌细胞的糖酵解代谢。此外,SMYD2是宫颈癌细胞生长所必需的,并且这种致癌作用主要依赖糖酵解。结论:SMYD2在宫颈癌细胞中过表达并在肿瘤代谢中发挥重要作用,能够通过调节有氧酵解促进宫颈癌细胞增殖。
文摘Electronic patient data gives many advantages,but also new difficulties.Deadlocks may delay procedures like acquiring patient information.Distributed deadlock resolution solutions introduce uncertainty due to inaccurate transaction properties.Soft computing-based solutions have been developed to solve this challenge.In a single framework,ambiguous,vague,incomplete,and inconsistent transaction attribute information has received minimal attention.The work presented in this paper employed type-2 neutrosophic logic,an extension of type-1 neutrosophic logic,to handle uncertainty in real-time deadlock-resolving systems.The proposed method is structured to reflect multiple types of knowledge and relations among transactions’features that include validation factor degree,slackness degree,degree of deadline-missed transaction based on the degree of membership of truthiness,degree ofmembership of indeterminacy,and degree ofmembership of falsity.Here,the footprint of uncertainty(FOU)for truth,indeterminacy,and falsity represents the level of uncertainty that exists in the value of a grade of membership.We employed a distributed real-time transaction processing simulator(DRTTPS)to conduct the simulations and conducted experiments using the benchmark Pima Indians diabetes dataset(PIDD).As the results showed,there is an increase in detection rate and a large drop in rollback rate when this new strategy is used.The performance of Type-2 neutrosophicbased resolution is better than the Type-1 neutrosophic-based approach on the execution ratio scale.The improvement rate has reached 10%to 20%,depending on the number of arrived transactions.
基金Supported by Program for Liaoning Excellent Talents in University (LJQ2011032)the National Natural Science Foundation of China (61203021)the National Science and Technology Support Program (2012BAF05B00)
文摘For dealing with large static error due to poor immunity of the traditional fuzzy control, a novel interval type-2 fuzzy control system is proposed. By extending the typical membership functions to interval type-2 membership functions, the proposed control system can efficiently reduce the uncertain disturbance from real environment without increasing the design complexity. The simulation results on the water tank level control system showed that the proposed method succeeded in better static and dynamic control with stronger robust performance than the traditional fuzzy control method.
基金supported by the National Natural Science Foundation of China(71771140)Project of Cultural Masters and“the Four Kinds of a Batch”Talents,the Special Funds of Taishan Scholars Project of Shandong Province(ts201511045)the Major Bidding Projects of National Social Science Fund of China(19ZDA080)。
文摘In view of the environment competencies,selecting the optimal green supplier is one of the crucial issues for enterprises,and multi-criteria decision-making(MCDM)methodologies can more easily solve this green supplier selection(GSS)problem.In addition,prioritized aggregation(PA)operator can focus on the prioritization relationship over the criteria,Choquet integral(CI)operator can fully take account of the importance of criteria and the interactions among them,and Bonferroni mean(BM)operator can capture the interrelationships of criteria.However,most existing researches cannot simultaneously consider the interactions,interrelationships and prioritizations over the criteria,which are involved in the GSS process.Moreover,the interval type-2 fuzzy set(IT2FS)is a more effective tool to represent the fuzziness.Therefore,based on the advantages of PA,CI,BM and IT2FS,in this paper,the interval type-2 fuzzy prioritized Choquet normalized weighted BM operators with fuzzy measure and generalized prioritized measure are proposed,and some properties are discussed.Then,a novel MCDM approach for GSS based upon the presented operators is developed,and detailed decision steps are given.Finally,the applicability and practicability of the proposed methodology are demonstrated by its application in the shared-bike GSS and by comparisons with other methods.The advantages of the proposed method are that it can consider interactions,interrelationships and prioritizations over the criteria simultaneously.
基金supported by the National Natural Science Foundation of China(61473048,61074093)
文摘In this paper, interval type-2 fuzzy sets, fuzzy comprehensive evaluation and the fuzzy control rules are synthesized to realize the control of unmanned vehicle in driving state and behavioral decisions. Compared to the type-1 fuzzy set, type-2 fuzzy sets have more advantages in handling the model based on uncertainties, linguistic information because the membership functions are fuzzy sets. Different membership functions are established for each factor when the unmanned vehicle is driving at different speed intervals. In addition, a new evaluation method is developed to analyze unmanned vehicle’s driving state. Finally, a set of dynamic fuzzy rules are sorted out, which can be applied to the unmanned vehicle’s behavioral decision-making and provide a new idea to related research.
文摘The modelling and formal characterization of spatial vagueness plays an increasingly important role in the imple- mentation of Geographic Information System (GIS). The concepts involved in spatial objects of GIS have been investigated and acknowledged as being vague and ambiguous. Models and methods which describe and handle fuzzy or vague (rather than crisp or determinate) spatial objects, will be more necessary in GIS. This paper proposes a new method for modelling spatial vagueness based on type-2 fuzzy set, which is distinguished from the traditional type-1 fuzzy methods and more suitable for describing and implementing the vague concepts and objects in GIS.
基金supported by the National Natural Science Foundation of China(7137115670971017)the Research Grants Council of the Hong Kong Special Administrative Region,China(City U112111)
文摘In order to measure the uncertain information of a type- 2 intuitionistic fuzzy set （T21FS）, an entropy measure of T21FS is presented by using the constructive principles. The proposed entropy measure is also proved to satisfy all of the constructive principles. Further, a novel concept of the type-2 triangular in- tuitionistic trapezoidal fuzzy set （T2TITrFS） is developed, and a geometric interpretation of the T2TITrFS is given to comprehend it completely or correctly in a more intuitive way. To deal with a more general uncertain complex system, the constructive principles of an entropy measure of T2TITrFS are therefore proposed on the basis of the axiomatic definition of the type-2 intuitionisic fuzzy entropy measure. This paper elicits a formula of type-2 triangular intuitionistic trapezoidal fuzzy entropy and verifies that it does sa- tisfy the constructive principles. Two examples are given to show the efficiency of the proposed entropy of T2TITrFS in describing the uncertainty of the type-2 intuitionistic fuzzy information and illustrate its application in type-2 triangular intuitionistic trapezodial fuzzy decision making problems.
