AIM To establish a severe acute cholangitis(SAC) model in mice.METHODS Cholecystic catheterization was performed under the condition of bile duct ligation(BDL). Trans-cholecystic injection of lipopolysaccharide(LPS) w...AIM To establish a severe acute cholangitis(SAC) model in mice.METHODS Cholecystic catheterization was performed under the condition of bile duct ligation(BDL). Trans-cholecystic injection of lipopolysaccharide(LPS) was defined as the SAC animal model. Sham operation group, intraperitoneal injection of LPS without BDL group, intraperitoneal injection of LPS with BDL group and trans-cholecystic injection of normal saline with BDL group were defined as control groups. The survival rates and tissue injuries in liver, lungs and kidney were evaluated.RESULTS Mice in the SAC group showed a time-dependent mortality and much more severe tissue injuries in liver, lungs and kidney, compared with other groups. However, relieving biliary obstruction could effectively reduce mortality and attenuate liver injury in the SAC mouse model.CONCLUSION Trans-cholecystic injection of LPS under the condition of biliary obstruction could establish a repeatable and reversible mouse model of SAC.展开更多
基金Supported by Zhejiang Provincial Natural Science Foundation of China under grant No.LY17H030001 and No.LQ14H160001Zhejiang Provincial Public Welfare Technology Application Research Projects under grant No.2015C33293,No.2013C33214 and No.2011C33023Research Foundation of Health Bureau of Zhejiang Province under grant No.2014RCA031 and No.2016KYB300
文摘AIM To establish a severe acute cholangitis(SAC) model in mice.METHODS Cholecystic catheterization was performed under the condition of bile duct ligation(BDL). Trans-cholecystic injection of lipopolysaccharide(LPS) was defined as the SAC animal model. Sham operation group, intraperitoneal injection of LPS without BDL group, intraperitoneal injection of LPS with BDL group and trans-cholecystic injection of normal saline with BDL group were defined as control groups. The survival rates and tissue injuries in liver, lungs and kidney were evaluated.RESULTS Mice in the SAC group showed a time-dependent mortality and much more severe tissue injuries in liver, lungs and kidney, compared with other groups. However, relieving biliary obstruction could effectively reduce mortality and attenuate liver injury in the SAC mouse model.CONCLUSION Trans-cholecystic injection of LPS under the condition of biliary obstruction could establish a repeatable and reversible mouse model of SAC.
