Research on fish sex determining genes and their mode of action is not only important for elucidating the mechanisms of vertebrate sex determination,but also for fish monosex control breeding.This paper briefly summar...Research on fish sex determining genes and their mode of action is not only important for elucidating the mechanisms of vertebrate sex determination,but also for fish monosex control breeding.This paper briefly summarizes our current knowledge about vertebrate sex determination,and reviews the progress of research on fish sex determining genes,including their function and their mechanism of action.In recent years,the number of newly uncovered fish sex determining genes has grown rapidly.In contrast,no sex determining genes have been identified in Cyprinid fishes,which constitute most of the aquaculture fish species in China.Technologies such as high-throughput sequencing and gene editing will provide important technical support for the study of sex determining genes in fish,and play an important role in establishing accurate sex-controlling breeding techniques and the creation of new fish germplasm.展开更多
Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane′s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cr...Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane′s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cranes and 15 youngs were identified. Through DNA sequence analysis, the identity is 94.77% between Red-crowned Crane and Oriental White Stork. The results of this study suggest that the application of the polymerase chain reaction technique is practicable for determining sex in the Red-crowned Crane.展开更多
基金supported by the National Key R&D Program of China(2018YFD0900203,2018YFD0901200)the National Natural Science Foundation of China(32030113)+2 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010108)Laboratory of Lingnan Modern Agriculture Project(NT2021008)Guangdong Basic and Applied Basic Research Foundation(2019B1515120072).
文摘Research on fish sex determining genes and their mode of action is not only important for elucidating the mechanisms of vertebrate sex determination,but also for fish monosex control breeding.This paper briefly summarizes our current knowledge about vertebrate sex determination,and reviews the progress of research on fish sex determining genes,including their function and their mechanism of action.In recent years,the number of newly uncovered fish sex determining genes has grown rapidly.In contrast,no sex determining genes have been identified in Cyprinid fishes,which constitute most of the aquaculture fish species in China.Technologies such as high-throughput sequencing and gene editing will provide important technical support for the study of sex determining genes in fish,and play an important role in establishing accurate sex-controlling breeding techniques and the creation of new fish germplasm.
文摘Sex determining gene primers of Oriental White Stork were used to amplify sex-linked gene of the Red-crowned Crane′s W chromosome-specific by PCR for sex identification. The sexes of 7 couples of grown Red-crowned Cranes and 15 youngs were identified. Through DNA sequence analysis, the identity is 94.77% between Red-crowned Crane and Oriental White Stork. The results of this study suggest that the application of the polymerase chain reaction technique is practicable for determining sex in the Red-crowned Crane.
