BACKGROUND Sex determining region Y-box 2(SOX2) can promote squamous cell carcinoma(SSC) because it regulates the migration and invasion of several different types of squamous carcinoma cells.However,few studies have ...BACKGROUND Sex determining region Y-box 2(SOX2) can promote squamous cell carcinoma(SSC) because it regulates the migration and invasion of several different types of squamous carcinoma cells.However,few studies have examined the prognostic value of SOX2 and its effect on the epithelial-mesenchymal transition(EMT) in esophageal SSC(ESCC),a cancer characterized by high invasion and rapid metastasis.AIM To verify the relationship of SOX2 and the EMT in ESCC and determine the prognostic value and significance of SOX2 and protein markers of the EMT in ESCC.METHODS One hundred and eighty-five postsurgical ESCC patients were retrospectively examined.Immunohistochemistry was used to detect SOX2,E-cadherin,and vimentin in ESCC tissues.The chi-square test was used to determine the relationships of the expression of these proteins with clinical data.Kaplan-Meier survival curves were used to evaluate factors associated with overall survival(OS).RESULTS SOX2 and vimentin had high expression in ESCC tissues and correlated with the depth of local carcinoma invasion.SOX2 expression had positive correlations with tumor size,vimentin expression,and the EMT,and a negative correlation with Ecadherin expression.Expression of SOX2 and vimentin had negative correlations with OS.SOX2 expression was an independent prognostic risk factor for poor OS in patients with ESCC.CONCLUSION SOX2 expression was an independent risk factor for OS in patients with ESCC and its expression had a positive correlation with the expression of vimentin,a classic marker of the EMT.SOX2 promoted the migration and invasion of ESCC,and this may related to its effect on vimentin in promoting the EMT.展开更多
目的探究性别决定区Y框蛋白9(sex determining region Y box protein 9,SOX9)在角膜内皮损伤过程中的表达及功能。方法通过转录组数据库分析人角膜内皮损伤与SOX9表达的关联。冷冻法构建小鼠角膜内皮损伤模型,每只小鼠左眼造模、右眼空...目的探究性别决定区Y框蛋白9(sex determining region Y box protein 9,SOX9)在角膜内皮损伤过程中的表达及功能。方法通过转录组数据库分析人角膜内皮损伤与SOX9表达的关联。冷冻法构建小鼠角膜内皮损伤模型,每只小鼠左眼造模、右眼空白对照,采用裂隙灯照相、眼前节光学相干断层扫描技术(optical coherence tomography,OCT)、免疫荧光技术评估动物模型质量,角膜铺片免疫荧光染色观察损伤后内皮细胞中SOX9的表达变化。分别用低浓度(30μmol/L)和高浓度(50μmol/L)的甲萘醌对人角膜内皮细胞(B4G12)处理3 h(短时间组)、6 h(中时间组)、12 h(长时间组)、24 h(超长时间组),构建细胞模型。采用显微镜照相、细胞计数试剂盒(cell counting kit-8,CCK-8)实验评估细胞模型质量,实时荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)、Western blot检测SOX9和介导角膜内皮-间质转化(endothelium-mesenchymal transition,EndMT)关键转录因子Snail家族转录抑制因子2(snail family transcriptional repressor 2,SNAIL2)的表达变化。结果数据库资料显示在角膜内皮损伤代表性疾病Fuchs角膜内皮营养不良(Fuchs′endothelial corneal dystrophy,FECD)患者中,FECD3期以上患者角膜内皮SOX9表达量比2期患者明显升高(P<0.05),且SOX9的下游基因富含半胱氨酸的酸性分泌蛋白(secreted protein acidic and rich in cysteine,SPARC)表达量比正常人明显升高(P<0.05)。在动物模型中,对照组角膜内皮铺片免疫荧光染色显示无SOX9荧光,损伤后短期出现大量SOX9荧光,损伤后中期、长期SOX9荧光回归极低水平。在甲萘醌处理的细胞模型中,SOX9表达随处理时间的延长呈现先升高后降低的趋势;同时发现SNAIL2的表达变化也会随甲萘醌处理时间的延长呈现先升高后降低的趋势。结论SOX9的表达随角膜内皮损伤进程产生变化,并与损伤修复中的EndMT紧密相关。展开更多
目的:探讨性别决定区Y框蛋白9(sex-determining region Y-box protein 9,SOX9)在甲状腺癌中的表达及其与患者临床病理特征及预后的关系。方法:回顾性分析2015年8月至2017年12月南阳市中心医院收治的98例甲状腺癌患者,收集患者根治术治...目的:探讨性别决定区Y框蛋白9(sex-determining region Y-box protein 9,SOX9)在甲状腺癌中的表达及其与患者临床病理特征及预后的关系。方法:回顾性分析2015年8月至2017年12月南阳市中心医院收治的98例甲状腺癌患者,收集患者根治术治疗过程中切除的甲状腺癌组织及癌旁组织分别作为甲状腺癌组与癌旁组。采用实时荧光定量PCR(real-time quantitative polymerase chain reaction,RT-qPCR)法检测甲状腺癌组织中SOX9 mRNA表达水平;免疫组织化学法检测SOX9蛋白表达。根据免疫组织化学染色结果中SOX9蛋白表达将甲状腺癌患者分为阳性表达组(67例)与阴性表达组(31例),观察其与患者临床病理特征的关系。对所有甲状腺癌患者进行3年随访,应用Kaplan-Meier法分析甲状腺癌患者3年累积生存率;采用Log-rank检验对影响患者3年总体生存期相关单因素进行分析;COX比例风险回归模型分析影响患者预后的多因素。结果:甲状腺癌组SOX9 mRNA及蛋白阳性表达率均显著高于癌旁组织(P<0.05);SOX9表达与淋巴结转移、TNM分期、包膜浸润明显相关(P<0.05);Kaplan-Meier法分析显示SOX9阴性表达组患者无进展生存期(progression-freesurvival,PFS)、总生存期(overall survival,OS)均显著高于阳性表达组(P<0.05);Log-rank分析显示高TNM分期、有淋巴结转移、有包膜浸润及SOX9阳性表达甲状腺癌患者平均生存时间明显缩短(P<0.05);COX多因素分析显示SOX9表达与淋巴结转移是影响甲状腺癌患者预后的独立危险因素。结论:甲状腺癌组织中SOX9表达水平明显升高,并可参与甲状腺癌发生及发展过程,SOX9高表达与患者预后差有关,可作为判断甲状腺癌病情进展及患者预后的重要指标。展开更多
基金Supported by National Natural Science Foundation of China,No. 81860422。
文摘BACKGROUND Sex determining region Y-box 2(SOX2) can promote squamous cell carcinoma(SSC) because it regulates the migration and invasion of several different types of squamous carcinoma cells.However,few studies have examined the prognostic value of SOX2 and its effect on the epithelial-mesenchymal transition(EMT) in esophageal SSC(ESCC),a cancer characterized by high invasion and rapid metastasis.AIM To verify the relationship of SOX2 and the EMT in ESCC and determine the prognostic value and significance of SOX2 and protein markers of the EMT in ESCC.METHODS One hundred and eighty-five postsurgical ESCC patients were retrospectively examined.Immunohistochemistry was used to detect SOX2,E-cadherin,and vimentin in ESCC tissues.The chi-square test was used to determine the relationships of the expression of these proteins with clinical data.Kaplan-Meier survival curves were used to evaluate factors associated with overall survival(OS).RESULTS SOX2 and vimentin had high expression in ESCC tissues and correlated with the depth of local carcinoma invasion.SOX2 expression had positive correlations with tumor size,vimentin expression,and the EMT,and a negative correlation with Ecadherin expression.Expression of SOX2 and vimentin had negative correlations with OS.SOX2 expression was an independent prognostic risk factor for poor OS in patients with ESCC.CONCLUSION SOX2 expression was an independent risk factor for OS in patients with ESCC and its expression had a positive correlation with the expression of vimentin,a classic marker of the EMT.SOX2 promoted the migration and invasion of ESCC,and this may related to its effect on vimentin in promoting the EMT.
文摘目的探究性别决定区Y框蛋白9(sex determining region Y box protein 9,SOX9)在角膜内皮损伤过程中的表达及功能。方法通过转录组数据库分析人角膜内皮损伤与SOX9表达的关联。冷冻法构建小鼠角膜内皮损伤模型,每只小鼠左眼造模、右眼空白对照,采用裂隙灯照相、眼前节光学相干断层扫描技术(optical coherence tomography,OCT)、免疫荧光技术评估动物模型质量,角膜铺片免疫荧光染色观察损伤后内皮细胞中SOX9的表达变化。分别用低浓度(30μmol/L)和高浓度(50μmol/L)的甲萘醌对人角膜内皮细胞(B4G12)处理3 h(短时间组)、6 h(中时间组)、12 h(长时间组)、24 h(超长时间组),构建细胞模型。采用显微镜照相、细胞计数试剂盒(cell counting kit-8,CCK-8)实验评估细胞模型质量,实时荧光定量聚合酶链式反应(real-time quantitative polymerase chain reaction,qPCR)、Western blot检测SOX9和介导角膜内皮-间质转化(endothelium-mesenchymal transition,EndMT)关键转录因子Snail家族转录抑制因子2(snail family transcriptional repressor 2,SNAIL2)的表达变化。结果数据库资料显示在角膜内皮损伤代表性疾病Fuchs角膜内皮营养不良(Fuchs′endothelial corneal dystrophy,FECD)患者中,FECD3期以上患者角膜内皮SOX9表达量比2期患者明显升高(P<0.05),且SOX9的下游基因富含半胱氨酸的酸性分泌蛋白(secreted protein acidic and rich in cysteine,SPARC)表达量比正常人明显升高(P<0.05)。在动物模型中,对照组角膜内皮铺片免疫荧光染色显示无SOX9荧光,损伤后短期出现大量SOX9荧光,损伤后中期、长期SOX9荧光回归极低水平。在甲萘醌处理的细胞模型中,SOX9表达随处理时间的延长呈现先升高后降低的趋势;同时发现SNAIL2的表达变化也会随甲萘醌处理时间的延长呈现先升高后降低的趋势。结论SOX9的表达随角膜内皮损伤进程产生变化,并与损伤修复中的EndMT紧密相关。
