AIM： To explore the molecular mechanisms in lens development and the pathogenesis of Peters anomaly in Smad4 defective mice. METHODS： Le-Cre transgenic mouse line was employed to inactivate Smad4 in the surface ect...AIM： To explore the molecular mechanisms in lens development and the pathogenesis of Peters anomaly in Smad4 defective mice. METHODS： Le-Cre transgenic mouse line was employed to inactivate Smad4 in the surface ectoderm selectively. Pathological techniques were used to reveal the morphological changes of the anterior segment in Smad4 defective eye. Immunohistochemical staining was employed to observe the expression of E-cadherin, N- cadherin and α-SMA in anterior segment of Smad4 defective mice and control mice at embryonic （E） day 16.5. Real-time quantitative polymerase chain reaction （qPCR） was performed to detect the expression of Snail, Zebl, Zeb2 and Twist2 in lens of Smad4 defective mice and control mice at E16.5. RESULTS： Conditional deletion of Smad4 on eye surface ectoderm resulted in corneal dysplasia, iridocorneal angle closure, corneolenticular adhesions and cataract resembling Peters anomaly. Loss of Smad4 function inhibited E-cadherin expression in the lens epithelium cells and corneal epithelium cells in Smad4 defective eye. Expression of N-cadherin was upregulated in corneal epithelium and corneal stroma. Both E-cadherin and N-cadherin were down-regulated at the future trabecular meshwork region in mutant eye. The qPCR results showed that the expression of Twist2 was increased significantly in the mutant lens （P〈0.01）. CONCLUSION： Smad4 is essential to eye development and likely a candidate pathogenic gene to Peters anomaly by regulating epithelial-mesenchymal transition. Twist2 can be regulated by Smad4 and plays an essential role in lens development.展开更多
This paper introduces a four-dimensional （4D） segmented disc dynamo which possesses coexisting hidden attractors with one stable equilibrium or a line equilibrium when parameters vary. In addition, by choosing an ap...This paper introduces a four-dimensional （4D） segmented disc dynamo which possesses coexisting hidden attractors with one stable equilibrium or a line equilibrium when parameters vary. In addition, by choosing an appropriate bifurcation parameter, the paper proves that Hopf bifurcation and pitchfork bifurcation occur in the system. The ultimate bound is also estimated. Some numerical investigations are also exploited to demonstrate and visualize the corresponding theoretical results.展开更多
In this work, the orthogonal cutting experiments on Ti-6Al-4V alloy were conducted at different cutting speeds(10—160 m/min)and feed rates(20—160 μm/rev). The tool-chip contact length was measured by the track of t...In this work, the orthogonal cutting experiments on Ti-6Al-4V alloy were conducted at different cutting speeds(10—160 m/min)and feed rates(20—160 μm/rev). The tool-chip contact length was measured by the track of tool rake face; meanwhile, the chip morphology caused by the localized and overall chip deformation was characterized by the degree of segmentation and the chip compression ratio, respectively. These parameters were analyzed and calculated according to the segmented chip morphology. In addition, three modified models considering the overall chip deformation and the localized deformation of adiabatic shear band were proposed, and the constants of the models were calculated by the genetic algorithm optimization. Considering the overall and localized chip deformation, the value and variation trend of the tool-contact length predicted by these three models agreed well with the experimental results.展开更多
Segmentation of semantic Video Object Planes (VOP's) from video sequence is a key to the standard MPEG-4 with content-based video coding. In this paper, the approach of automatic Segmentation of VOP's Based on...Segmentation of semantic Video Object Planes (VOP's) from video sequence is a key to the standard MPEG-4 with content-based video coding. In this paper, the approach of automatic Segmentation of VOP's Based on Spatio-Temporal Information (SBSTI) is proposed.The proceeding results demonstrate the good performance of the algorithm.展开更多
基金Supported by the National Natural Science Foundation of China(No.81470617No.81371003)Colleges and Universities Scientific Research Project of Liaoning Province,China(No.L2014305)
文摘AIM： To explore the molecular mechanisms in lens development and the pathogenesis of Peters anomaly in Smad4 defective mice. METHODS： Le-Cre transgenic mouse line was employed to inactivate Smad4 in the surface ectoderm selectively. Pathological techniques were used to reveal the morphological changes of the anterior segment in Smad4 defective eye. Immunohistochemical staining was employed to observe the expression of E-cadherin, N- cadherin and α-SMA in anterior segment of Smad4 defective mice and control mice at embryonic （E） day 16.5. Real-time quantitative polymerase chain reaction （qPCR） was performed to detect the expression of Snail, Zebl, Zeb2 and Twist2 in lens of Smad4 defective mice and control mice at E16.5. RESULTS： Conditional deletion of Smad4 on eye surface ectoderm resulted in corneal dysplasia, iridocorneal angle closure, corneolenticular adhesions and cataract resembling Peters anomaly. Loss of Smad4 function inhibited E-cadherin expression in the lens epithelium cells and corneal epithelium cells in Smad4 defective eye. Expression of N-cadherin was upregulated in corneal epithelium and corneal stroma. Both E-cadherin and N-cadherin were down-regulated at the future trabecular meshwork region in mutant eye. The qPCR results showed that the expression of Twist2 was increased significantly in the mutant lens （P〈0.01）. CONCLUSION： Smad4 is essential to eye development and likely a candidate pathogenic gene to Peters anomaly by regulating epithelial-mesenchymal transition. Twist2 can be regulated by Smad4 and plays an essential role in lens development.
基金supported by the National Natural Science Foundation of China(Grant No.11671149)
文摘This paper introduces a four-dimensional （4D） segmented disc dynamo which possesses coexisting hidden attractors with one stable equilibrium or a line equilibrium when parameters vary. In addition, by choosing an appropriate bifurcation parameter, the paper proves that Hopf bifurcation and pitchfork bifurcation occur in the system. The ultimate bound is also estimated. Some numerical investigations are also exploited to demonstrate and visualize the corresponding theoretical results.
基金Supported by the National Natural Science Foundation of China(No.51205284 and No.51575384)
文摘In this work, the orthogonal cutting experiments on Ti-6Al-4V alloy were conducted at different cutting speeds(10—160 m/min)and feed rates(20—160 μm/rev). The tool-chip contact length was measured by the track of tool rake face; meanwhile, the chip morphology caused by the localized and overall chip deformation was characterized by the degree of segmentation and the chip compression ratio, respectively. These parameters were analyzed and calculated according to the segmented chip morphology. In addition, three modified models considering the overall chip deformation and the localized deformation of adiabatic shear band were proposed, and the constants of the models were calculated by the genetic algorithm optimization. Considering the overall and localized chip deformation, the value and variation trend of the tool-contact length predicted by these three models agreed well with the experimental results.
文摘Segmentation of semantic Video Object Planes (VOP's) from video sequence is a key to the standard MPEG-4 with content-based video coding. In this paper, the approach of automatic Segmentation of VOP's Based on Spatio-Temporal Information (SBSTI) is proposed.The proceeding results demonstrate the good performance of the algorithm.
