Sucrose non-fermenting-1-related protein kinase-1(SnRK1)regulates carbon and nitrogen metabolism in plants.However,its roles and their underlying mechanisms in tolerance to abiotic stresses are little known.The presen...Sucrose non-fermenting-1-related protein kinase-1(SnRK1)regulates carbon and nitrogen metabolism in plants.However,its roles and their underlying mechanisms in tolerance to abiotic stresses are little known.The present study indicated that the IbSnRK1 gene was strongly induced by Na Cl,polyethylene glycol(PEG)6000,hydrogen peroxide(H2O2),cold(4°C),and abscisic acid(ABA).Its overexpression significantly increased salt,drought,and cold tolerance in transgenic sweet potato plants.ABA,proline,and K+contents were significantly increased,whereas malondialdehyde(MDA),Na+and H2O2 contents and O2-production rate were significantly decreased in the transgenic plants under salt,drought,and cold stresses.Overexpression of the gene up-regulated genes involved in ABA biosynthesis,stress response,and stomatal closure;increased enzyme activities in the reactive oxygen species(ROS)scavenging system;and controlled stomatal closure under salt,drought,and cold stresses.These results show that the IbSnRK1 gene confers salt,drought,and cold tolerance in sweet potato by activating the ROS scavenging system and controlling stomatal closure via the ABA signaling pathway.展开更多
A complete cDNA of potato Phytophthora infestans-induced hypersensitive response-related protein gene (POTHR-1) was cloned using rapid amplification of cDNA ends (RACE) strategy according to a fragment sequence which ...A complete cDNA of potato Phytophthora infestans-induced hypersensitive response-related protein gene (POTHR-1) was cloned using rapid amplification of cDNA ends (RACE) strategy according to a fragment sequence which we had cloned using suppression subtractive hybridization (SSH) technique. The potato POTHR-1 gene encodes a protein of 225 amino acids, which shares 81% identity with tobacco hin1 gene-enoded protein (harpin-induced protein). Southern blot revealed that there are two to three copies of POTHR-1 in potato genome. The POTHR-1 gene expression in potato leaves showed that its transcripts accumulated remarkably in leaves after 36 h inoculation with P. infestans. Mechanical wounding and jasmonic acid (JA) could induce the POTHR-1 gene expression and osmotic stress just induce a slight accumulation of POTHR-1 gene mRNA, while salicylic acid (SA) had no detectable function on the induction accumulation of POTHR-1 gene transcripts. The potato POTHR-1 gene may preferentially associate with hypersensitive response (HR) or biotic cell death during interaction between host and pathogen.展开更多
[Objective] The research aimed to study the effects of 1-methylcyclopropene （1-MCP） and chitosan on active oxygen metabolism and quality of fresh-cut potato during storage. [Method] The fresh-cut potato slices were ...[Objective] The research aimed to study the effects of 1-methylcyclopropene （1-MCP） and chitosan on active oxygen metabolism and quality of fresh-cut potato during storage. [Method] The fresh-cut potato slices were respectively treated with 1- MCP （2 μl/L）, chitosan or 1-MCP ＋ chitosan. During the storage period, the contents of superoxide anion （02）, malondialdehyde （MDA）, hydrogen dioxide （H202） and Vita- min C （Vc）, the activities of polyphenol oxidase （PPO）, peroxydase （POD） and super- oxide dismutase （SOD） as well as the respiratory rate of the fresh-cut potatoes in all the treatments were determined every day. The fresh-cut potato slices treated without any reagents were used as control. [Result] 1-MCP Treated could significantly de- creased the respiratory rate, PPO activity and the accumulations of 02 , H202 and MDA, increased the activities of SOD and POD and slowed down Vc content reduc- tion. On the contrary, potato treated with chitosan significantly inhibited the POD activi- ty in fresh-cut potato. Compared with the control, the combination of 1-MCP and chi- tosan showed a little but not significant better effects on potato preservation. [Conclu- sion] 1-MCP Treated showed the best effects on potato preservation.展开更多
The aim of this study was to investigate the effect of different concentrations of sporamin on the differentiation and proliferation of 3T3-LI preadipocytes, providing the theoretical basis for the development of food...The aim of this study was to investigate the effect of different concentrations of sporamin on the differentiation and proliferation of 3T3-LI preadipocytes, providing the theoretical basis for the development of food to treat obesity and diabetes, The isolation and purification of sporamin from sweet potato species 55-2 were performed by ammonium sulphate precipitation in combination with ion-exchange and gel filtration chromatography. With berberine as a positive control, different concentrations ofsporamin （0.000, 0.125, 0.025, 0.250, 0.500, and 1.000 mg·mL^-1 were used to treat 3T3-L1 preadipocytes. Intracellular fat accumulation and the degree of adipogenesis were quantified using Oil Red O staining and colorimetry, Preadipocytes differentiation was measured by 3（4,5-dimethylthiazolyl-2-yl）-2,5-diphenyltetrazolium bromide （MTT） spectrophotometric assay. Two sporamin proteins, which were separated into sporamin A （31 kD） and sporamin B （22 kD）, could be purified by ion-exchange and gel filtration chromatography. After being treated by different concentrations of sporamin, the differentiation of 3T3-L1 preadipocytes was significantly inhibited, compared with the positive control. When the sporamin solution concentration was 0.500 mg mL-1, the accumulation of lipid droplets within the cells was significantly decreased and the optical density （OD） value of the solution from destained Oil Red O reached to 0.35, which was the lowest value （P〈 0.05）. The proliferation of 3T3-L1 preadipocytes was significantly inhibited by treating at higher sporamin concentrations. In addition, the inhibitory effect was more obvious with the prolonged treatment time （P〈 0.05）. The differentiation and proliferation of 3T3-L1 preadipocytes could be inhibited significantly by the addition of higher concentration sporamin. It was, therefore, suggested that the sporamin was potentially effective for weight loss.展开更多
Salt stress seriously restricts the growth and yield of potatoes.Plant cystatins are vital players in biotic stress and development,however,their roles in salt stress resistance remain elusive.Here,we report that StCY...Salt stress seriously restricts the growth and yield of potatoes.Plant cystatins are vital players in biotic stress and development,however,their roles in salt stress resistance remain elusive.Here,we report that StCYS1 positively regulates salt tolerance in potato plants.An in vitro biochemical test demonstrated that StCYS1 is a bona fide cystatin.Overexpression of StCYS1 in both Escherichia coli and potato plants significantly increased their resistance to high salinity.Further analysis revealed that the transgenic plants accumulated more proline and chlorophyll under salt stress conditions.Moreover,the transgenic plants displayed higher H2O2 scavenging capability and cell membrane integrity compared with wild-type potato.These results demonstrate that StCYS1 is closely correlated with salt stress and its overaccumulation can substantially enhance salt stress resistance.展开更多
基金supported by the National Key Research and Development Program of China(2019YFD1001303 and 2019YFD1001300)China Agriculture Research System(CARS-10,Sweetpotato)。
文摘Sucrose non-fermenting-1-related protein kinase-1(SnRK1)regulates carbon and nitrogen metabolism in plants.However,its roles and their underlying mechanisms in tolerance to abiotic stresses are little known.The present study indicated that the IbSnRK1 gene was strongly induced by Na Cl,polyethylene glycol(PEG)6000,hydrogen peroxide(H2O2),cold(4°C),and abscisic acid(ABA).Its overexpression significantly increased salt,drought,and cold tolerance in transgenic sweet potato plants.ABA,proline,and K+contents were significantly increased,whereas malondialdehyde(MDA),Na+and H2O2 contents and O2-production rate were significantly decreased in the transgenic plants under salt,drought,and cold stresses.Overexpression of the gene up-regulated genes involved in ABA biosynthesis,stress response,and stomatal closure;increased enzyme activities in the reactive oxygen species(ROS)scavenging system;and controlled stomatal closure under salt,drought,and cold stresses.These results show that the IbSnRK1 gene confers salt,drought,and cold tolerance in sweet potato by activating the ROS scavenging system and controlling stomatal closure via the ABA signaling pathway.
文摘A complete cDNA of potato Phytophthora infestans-induced hypersensitive response-related protein gene (POTHR-1) was cloned using rapid amplification of cDNA ends (RACE) strategy according to a fragment sequence which we had cloned using suppression subtractive hybridization (SSH) technique. The potato POTHR-1 gene encodes a protein of 225 amino acids, which shares 81% identity with tobacco hin1 gene-enoded protein (harpin-induced protein). Southern blot revealed that there are two to three copies of POTHR-1 in potato genome. The POTHR-1 gene expression in potato leaves showed that its transcripts accumulated remarkably in leaves after 36 h inoculation with P. infestans. Mechanical wounding and jasmonic acid (JA) could induce the POTHR-1 gene expression and osmotic stress just induce a slight accumulation of POTHR-1 gene mRNA, while salicylic acid (SA) had no detectable function on the induction accumulation of POTHR-1 gene transcripts. The potato POTHR-1 gene may preferentially associate with hypersensitive response (HR) or biotic cell death during interaction between host and pathogen.
文摘[Objective] The research aimed to study the effects of 1-methylcyclopropene （1-MCP） and chitosan on active oxygen metabolism and quality of fresh-cut potato during storage. [Method] The fresh-cut potato slices were respectively treated with 1- MCP （2 μl/L）, chitosan or 1-MCP ＋ chitosan. During the storage period, the contents of superoxide anion （02）, malondialdehyde （MDA）, hydrogen dioxide （H202） and Vita- min C （Vc）, the activities of polyphenol oxidase （PPO）, peroxydase （POD） and super- oxide dismutase （SOD） as well as the respiratory rate of the fresh-cut potatoes in all the treatments were determined every day. The fresh-cut potato slices treated without any reagents were used as control. [Result] 1-MCP Treated could significantly de- creased the respiratory rate, PPO activity and the accumulations of 02 , H202 and MDA, increased the activities of SOD and POD and slowed down Vc content reduc- tion. On the contrary, potato treated with chitosan significantly inhibited the POD activi- ty in fresh-cut potato. Compared with the control, the combination of 1-MCP and chi- tosan showed a little but not significant better effects on potato preservation. [Conclu- sion] 1-MCP Treated showed the best effects on potato preservation.
基金supported by the National 863 Program of China (2006AA10Z332)the Ear Marked Fund for Modern Agro-Industry Technology Research System, China
文摘The aim of this study was to investigate the effect of different concentrations of sporamin on the differentiation and proliferation of 3T3-LI preadipocytes, providing the theoretical basis for the development of food to treat obesity and diabetes, The isolation and purification of sporamin from sweet potato species 55-2 were performed by ammonium sulphate precipitation in combination with ion-exchange and gel filtration chromatography. With berberine as a positive control, different concentrations ofsporamin （0.000, 0.125, 0.025, 0.250, 0.500, and 1.000 mg·mL^-1 were used to treat 3T3-L1 preadipocytes. Intracellular fat accumulation and the degree of adipogenesis were quantified using Oil Red O staining and colorimetry, Preadipocytes differentiation was measured by 3（4,5-dimethylthiazolyl-2-yl）-2,5-diphenyltetrazolium bromide （MTT） spectrophotometric assay. Two sporamin proteins, which were separated into sporamin A （31 kD） and sporamin B （22 kD）, could be purified by ion-exchange and gel filtration chromatography. After being treated by different concentrations of sporamin, the differentiation of 3T3-L1 preadipocytes was significantly inhibited, compared with the positive control. When the sporamin solution concentration was 0.500 mg mL-1, the accumulation of lipid droplets within the cells was significantly decreased and the optical density （OD） value of the solution from destained Oil Red O reached to 0.35, which was the lowest value （P〈 0.05）. The proliferation of 3T3-L1 preadipocytes was significantly inhibited by treating at higher sporamin concentrations. In addition, the inhibitory effect was more obvious with the prolonged treatment time （P〈 0.05）. The differentiation and proliferation of 3T3-L1 preadipocytes could be inhibited significantly by the addition of higher concentration sporamin. It was, therefore, suggested that the sporamin was potentially effective for weight loss.
基金We thank Li Guangcun(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences)for providing the potato(Solanum tuberosumL.)cultivar MDS.This work was supported by the National Natural Science Foundation of China(31901752)by a grant from the Potato Industry lnnovation Team for Modern Agricultural Industry Technology System,Shandong Province,China(SDAIT-10-011-11).
文摘Salt stress seriously restricts the growth and yield of potatoes.Plant cystatins are vital players in biotic stress and development,however,their roles in salt stress resistance remain elusive.Here,we report that StCYS1 positively regulates salt tolerance in potato plants.An in vitro biochemical test demonstrated that StCYS1 is a bona fide cystatin.Overexpression of StCYS1 in both Escherichia coli and potato plants significantly increased their resistance to high salinity.Further analysis revealed that the transgenic plants accumulated more proline and chlorophyll under salt stress conditions.Moreover,the transgenic plants displayed higher H2O2 scavenging capability and cell membrane integrity compared with wild-type potato.These results demonstrate that StCYS1 is closely correlated with salt stress and its overaccumulation can substantially enhance salt stress resistance.
