The biosafety level-2 macromolecular crystallography beamline(BL10U2)at the Shanghai Synchrotron Radiation Facility

BL10U2 is an undulator-based macromolecular crystallography(MX)beamline located at the 3.5-GeV Shanghai Synchrotron Radiation Facility.BL10U2 is specifically designed for conducting routine and biosafety level-2(BSL-2)MX experiments utilizing high-flux tunable X-rays with energies from 7 to 18 keV,providing a beam spot size of 20μm(horizontal)×10μm(vertical)at the sample point.Certification by the Shanghai Pudong Municipal Health Commission confirmed the capability to perform BSL-2 MX experiments.The beamline is currently equipped with an Eiger X 16 M detector and two newly developed in-house high-precision diffractometers that can be switched to perform conventional or in situ crystal diffraction experiments.An automatic sample changer developed in-house allows fast sample exchange in less than 30 s,supporting high-throughput MX experimentation and rapid crystal screening.Data collection from both the diffractometer and detector was controlled by an in-house developed data collection software(Finback)with a user-friendly interface for convenient operation.This study presents a comprehensive overview of the facilities,experimental methods,and performance characteristics of the BL10U2 beamline.

BL02U1:the relocated macromolecular crystallography beamline at the Shanghai Synchrotron Radiation Facility

Macromolecular crystallography beamline BL17U1 at the Shanghai Synchrotron Radiation Facility has been relocated,upgraded,and given a new ID(BL02U1).It now delivers X-rays in the energy range of 6–16 keV,with a focused beam of 11.6μm×4.8μm and photon flux greater than 1012 phs/s.The high credibility and stability of the beam and good timing synchronization of the equipment significantly improve the experimental efficiency.Since June 2021,when it officially opened to users,over 4200 h of beamtime have been provided to over 200 research groups to collect data at the beamline.Its good performance and stable operation have led to the resolution of several structures based on data collected at the beamline.

The protein complex crystallography beamline(BL19U1)at the Shanghai Synchrotron Radiation Facility

The protein complex crystallographic beamline BL19U1 at the Shanghai Synchrotron Radiation Facility is one of the five beamlines dedicated to protein sciences operated by National Facility for Protein Science(Shanghai,China).The beamline,which features a small-gap invacuum undulator,has been officially open to users since March 2015.This beamline delivers X-ray in the energy range 7–15 keV.With its high flux,low divergence beam and a large active area detector,BL19U1 is designed for proteins with large molecular weight and large crystallographic unit cell dimensions.Good performance and stable operation of the beamline have allowed the number of Protein Data Bank(PDB)depositions and the number of articles published based on data collected at this beamline to increase steadily.To date,over 300 research groups have collected data at the beamline.More than 600 PDB entries have been deposited at the PDB(www.pdb.org).More than 300 papers have been published that include data collected at the beamline,including 21 research articles published in the top-level journals Cell,Nature,and Science.

Design of wide-range energy material beamline at the Shanghai Synchrotron Radiation Facility

We report the design of a wide-range energy material beamline(E-line) with multiple experimental techniques at the Shanghai Synchrotron Radiation Facility.The undulators consisted of an elliptically polarizing undulator and in-vacuum undulator that generate the soft and hard X-rays, respectively. The beamline covered a wide energy range from 130 to 18 ke V with both a high photon flux([ 10^(12) phs/s with exit silt 30 lm in soft X-ray and [ 5 9 10^(12) phs/s in hard X-ray within 0.1%BW bandwidth) and promising resolving power(maximum E/DE [ 15,000 in soft X-ray with exit silt 30 lm and [6000 in hard X-ray). Moreover, the beam spots from the soft and hard X-rays were focused to the same sample position with a high overlap ratio, so that the surfaces, interfaces, and bulk properties were characterized in situ by changing the probing depth.

Tuning control system of a third harmonic superconducting cavity in the Shanghai Synchrotron Radiation Facility

Beam lifetime is dominated by Touschek scattering at the Shanghai Synchrotron Radiation Facility(SSRF).Touschek loss rate is affected by probability for scattering beyond the RF acceptance and the volume charge density of the bench.In the phaseⅡupgrade of the SSRF,a third harmonic superconducting cavity will be used to enhance the Touschek lifetime by lengthening the bunches.The Touschek lifetime improvement factor is affected by the voltage of a harmonic cavity.To stabilize the cavity voltage,a tuning control system was designed to control it.The design of the tuning control system was based on the SSRF third-generation low-level RF control system.Some hardware and specialized algorithms were redesigned to fit the harmonic cavity control.The design of the tuning control system is complete,and the control system has been tested.The test result shows that the fluctuation of amplitude is<±0.34%within 1.5 h,which satisfies the stability requirement.

Synchrotron radiation-based materials characterization techniques shed light on molten salt reactor alloys

From a safety point of view, it is important to study the damages and reliability of molten salt reactor structural alloy materials, which are subjected to extreme environments due to neutron irradiation, molten salt corrosion, fission product attacks, thermal stress, and even combinations of these. In the past few years, synchrotron radiation-based materials characterization techniques have proven to be effective in revealing the microstructural evolution and failure mechanisms of the alloys under surrogating operation conditions. Here, we review the recent progress in the investigations of molten salt corrosion,tellurium(Te) corrosion, and alloy design. The valence states and distribution of chromium(Cr) atoms, and the diffusion and local atomic structure of Te atoms near the surface of corroded alloys have been investigated using synchrotron radiation techniques, which considerably deepen the understandings on the molten salt and Te corrosion behaviors. Furthermore, the structure and size distribution of the second phases in the alloys have been obtained, which are helpful for the future development of new alloy materials.

The SLEGS beamline of SSRF

The Shanghai Laser Electron Gamma Source(SLEGS, located in BL03SSID) beamline at the Shanghai Synchrotron Radiation Facility(SSRF) is a Laser Compton Scattering(LCS) gamma source used for the investigation of nuclear structure, which is in extensive demand in fields such as nuclear astrophysics, nuclear cluster structure, polarization physics, and nuclear energy. The beamline is based on the inverse Compton scattering of 10640 nm photons on 3.5 GeV electrons and a gamma source with variable energy by changing the scattering angle from 20° to 160°. γ rays of 0.25-21.1 MeV can be extracted by the scheme consisting of the interaction chamber, coarse collimator, fine collimator, and attenuator. The maximum photon flux for 180° is approximately 10~7 photons/s at the target at 21.7 MeV, with a 3-mm-diameter beam. The beamline was equipped with four types of spectrometers for experiments in( γ,γ'),( γ,n),( γ,p), and( γ,α). At present, Nuclear Resonance Fluorescence(NRF) spectrometry, Flat-Efficiency neutron Detector(FED) spectrometry, neutron Time-Of-Flight(TOF) spectrometry, and Light-Charged Particle(LCP) spectrometry methods have been developed.

Ultrahard X-ray multifunctional application beamline at the SSRF

The ultrahard X-ray multifunctional application beamline(BL12SW)is a phase-II beamline project at the Shanghai Syn-chrotron Radiation Facility.The primary X-ray techniques used at the beamline are high-energy X-ray diffraction and imaging using white and monochromatic light.The main scientific objectives of ultrahard X-ray beamlines are focused on two research areas.One is the study of the structural properties of Earth’s interior and new materials under extreme high-temperature and high-pressure conditions,and the other is the characterization of materials and processes in near-real service environments.The beamline utilizes a superconducting wiggler as the light source,with two diamond windows and SiC discs to filter out low-energy light(primarily below 30 keV)and a Cu filter assembly to control the thermal load entering the subsequent optical components.The beamline is equipped with dual monochromators.The first was a meridional bending Laue monochromator cooled by liquid nitrogen,achieving a full-energy coverage of 30-162 keV.The second was a sagittal bending Laue monochromator installed in an external building,providing a focused beam in the horizontal direction with an energy range of 60-120 keV.There were four experimental hutches:two large-volume press experimental hutches(LVP1 and LVP2)and two engineering material experimental hutches(ENG1 and ENG2).Each hutch was equipped with various near-real service conditions to satisfy different requirements.For example,LVP1 and LVP2 were equipped with a 200-ton DDIA press and a 2000-ton dual-mode(DDIA and Kawai)press,respectively.ENG1 and ENG2 provide in situ tensile,creep,and fatigue tests as well as high-temperature conditions.Since June 2023,the BL12SW has been in trial operation.It is expected to officially open to users by early 2024.

The hard X-ray nanoprobe beamline at the SSRF

The hard X-ray nanoprobe beamline BL13U is a phase-Ⅱ beamline project at the Shanghai Synchrotron Radiation Facility.The beamline aims to enable comprehensive experiments at high spatial resolutions ranging from 50 to 10 nm. The X-ray energy range of the beamline, 5–25 keV, can detect most elements in the periodic table. Two operating modes were designed to accommodate the experimental requirements of high-energy resolution or high photon flux, respectively. X-ray nanofluorescence, nanodiffraction, and coherent diffraction imaging are developed as the main experimental techniques for BL13U. This paper describes the beamline optics, end station configurations, experimental methods under development, and preliminary test results. This comprehensive overview aims to provide a clear understanding of the beamline capabilities and potential applications.

The new X-ray imaging and biomedical application beamline BL13HB at SSRF

A new X-ray imaging and biomedical application beamline(BL13HB)has been implemented at the Shanghai Radiation Synchrotron Facility(SSRF)as an upgrade to the old X-ray imaging and biomedical application beamline(BL13W1).This is part of the Phase II construction project of the SSRF.The BL13HB is dedicated to 2D and 3D static and dynamic X-ray imaging,with a field of view of up to 48.5 mm×5.2 mm and spatial resolution as high as 0.8μm.A super-bending magnet is used as the X-ray source in BL13HB,which has a maximum magnetic field of 2.293 T.The energy range of monochromatic X-ray photons from a double-multiplayer monochromator was 8–40 keV,and the white beam mode was provided on the beamline for dynamic X-ray imaging and dynamic X-ray micro-CT.While maintaining the previous experimental setup of BL13W1,new equipment was added to the beamline experimental station.The beamline is equipped with different sets of X-ray imaging detectors for several experimental methods such as micro-CT,dynamic micro-CT,and pair distribution function.The experimental station of BL13HB is designed specifically for various in situ dynamic experiments,and BL13HB has been open to users since June 2021.

同步辐射光源新增线站相关加速器安装与调试

以上海光源新增的双插入件04IVU波荡器和04Wigger扭摆器为例,介绍了已运行的同步辐射光源新增束线工程中加速器方面需要着重处理的工作。首先,介绍插入件的设计参数及磁测结果,并使用SPECTRA程序计算了其光谱亮度;详细梳理了插入件的安装过程遇到的真空盒工艺误差、安装任务冲突和项目安装超期等问题及其解决方案;阐述了插入件调试工作的物理机制,以及如何应用前馈补偿的方法抑制了插入件调试过程中束流轨道影响,双插入件IVU和Wiggler的最高亮度分别为3 keV时的5.86×10^(19)Photon·s^(−1)·mm^(−2)·mrad^(−2)·(0.1%B.W.)^(−1)和2.75×10^(15)Photon·s^(−1)·(0.1%B.W.)^(−1),校正后IVU和Wiggler最大轨道扰动均小于2μm。对前端区真空清洗工作的意义、方法和结果进行了分析,并系统性地介绍了主被动模式相结合的新方法,该方法实现了高效的真空清洗。归纳了试运行过程中遇到的联锁逻辑错误、前端区真空异常、安全光闸硬联锁等问题,并总结了应对措施,为双插入件更好投入运行提供帮助。通过这些工作的进行,该双插入件已完成安装并基本调试完毕,对其他光源新增线站相关加速器安装与调试具有一定的参考价值。

SSRF磁测数据管理与应用软件系统的开发

介绍了上海同步辐射装置即上海光源工程(简称SSRF)磁测数据的管理与应用软件系统的研发情况。

EPICS在SSRF液氮冷却单色器运动控制中的应用

介绍了上海光源液氮冷却单色器的工作原理,描述了在EPICS环境下实现单色器步进电机控制、能量扫描和探测器信号实时获取、晶体切换和高度补偿以及单色器姿态参数保存等功能。实验结果表明,单色器能够提供满足实验要求的同步辐射光,运动控制系统的各项指标达到或优于设计目标。

Highly coupled off-resonance lattice design in diffraction-limited light sources

The round-beam operation presents many benefits for scientific experiments regarding synchrotron radiation and the weak-ening influences of intra-beam scattering in diffraction-limited synchrotron light sources.A round-beam generation method based on the global setting of skew quadrupoles and the application of a non-dominated sorting genetic algorithm was pro-posed in this study.Two schemes,including large-emittance coupling introduced via betatron coupling and vertical disper-sion,were explored in a candidate lattice for an upgrade-proposal of the Shanghai synchrotron radiation facility.Emittance variations with lattice imperfections and their influence on the beam dynamics of beam optic distortions were investigated.The results demonstrated that a precise coupling control ranging from 10 to 100%was achieved under low optical distortion,whereas full-coupling generation and its robustness were achieved by our proposed method by adjusting the skew quadrupole components located in the dispersion-free sections.The Touschek lifetime increased by a factor of 2–2.5.

基于EPICS的SSRF-X射线小角散射实验站运动控制系统

运动控制系统是上海光源(SSRF)X射线小角散射(SAXS)实验站建设的重要组成部分,该控制系统通过对实验站运动部件的远程精确控制来实现对光斑的准直调节和寻找最佳的散射实验样品放置位置,以满足不同用户的实验环境要求。该系统采用国际加速器控制界称为"标准模型"的分布式控制体系结构,应用EPICS软件实现了对实验站的23个运动部件的远程运动控制,被控设备包括两个四刀口单色光狭缝及其三维狭缝支撑平台、七维样品台和两个Beam-Stop。运动测试结果表明:系统的运动分辨率最大为20μm,最小为0.33333μm,运动误差约为1%。测试结果满足小角散射实验站运动控制的要求,具有高的稳定性、可重复性和精度。目前该系统已成功应用于SSRF-SAXS实验站的调试运行。

Upgrade of macromolecular crystallography beamline BL17U1at SSRF

Beamline BL17 U1 at Shanghai Synchrotron Radiation Facility is an energy-tunable macromolecular crystallography beamline that has been in user operation since 2009. Growing demand from the user community for a small beam and related experimental methods have motivated upgrades of the devices in the endstation.Minibeam modes have already been developed for operation. A self-integrated diffractometer reduces the sphere of confusion of the rotatory axis to 1μm. The new diffractometer is equipped with an upgraded on-axis viewing system that can improve the resolving power. Additionally,the area detector was also upgraded to the newest generation of detectors, the EIGER X 16 M, which can collect data at 133 Hz. After these upgrades, the endstation became virtually new. This paper covers the upgrade of the endstation devices and gives the first data collection results.

Current status and progresses of SSRF project

The Shanghai Synchrotron Radiation Facility (SSRF), a third generation light source, comprises a 3.5GeV electron storage ring, a full energy booster, a 150 MeV linac, and seven beamlines in Phase I of the project. Beginning at the end of 2004 with a groundbreaking ceremony, the accelerators were installed in ten months from November 2006, and were successfully tested and commissioned in the past a couple of months. On December 21, 2007, storing electron beams in the storage ring was realized, and the first synchrotron radiations were observed three days later on the front-end of Beamline BL16B of the facility. Now, it runs 3 GeV 100 mA beams with a lifetime of 8~10 hours. Meanwhile, construction of the first seven beamlines (five ID beamlines and two bending magnet beamlines) is progressing on schedule.

Development of the bunch-by-bunch beam current acquisition system at SSRF

In this paper,we report the development of a bunch-by-bunch beam current acquisition system.Through a waveform-reconstruction algorithm,the system realizes high equivalent sampling rate with a relatively low inherent rate.Based on the EPICS environment,information communication with other systems can be achieved.Preliminary test results in commissioning the SSRF storage ring show that the system can reconstruct the beam waveform of single bunch,providing a convenient and reliable method for the top-up operation in the future.

BL19U2:Small-angle X-ray scattering beamline for biological macromolecules in solution at SSRF

The BL19U2 at the Shanghai Synchrotron Radiation Facility is a small-angle X-ray scattering beamline dedicated to structural studies pertaining to biological macromolecules in solution.The beamline has been officially opened to users in March 2015,and since then,a series of technological innovations has been developed to optimize beamline performance,thereby significantly improving the data collection efficiency and broadening the application scope of biological small-angle X-ray scattering.BL19U2 is ideal for the high-throughput screening of weakly scattered proteins,protein assemblies,nucleic acids,inorganic nanomaterials,and organic drug molecules.This paper describes the design and overview of the BL19U2 beamline.Versatile sample environments at the experimental station and some recent scientific highlights are presented.

Feedforward compensation of the insertion devices effects in the SSRF storage ring

The lattice of the Shanghai Synchrotron Radiation Facility(SSRF) storage ring was upgraded in the Phase-II beamline project, and thus far, 18 insertion devices(IDs) have been installed. The IDs cause closed-orbit distortions, tune drift, and coupling distortions in the SSRF storage ring, all of which are significant issues that require solutions. In this study, an ID orbit feedforward compensation system based on a response matrix using corrector coils was developed, and it was applied to all commissioned IDs in the SSRF storage ring. After correction, the maximum ID-induced horizontal and vertical orbit distortions were less than 5.0 and 3.5 μm, respectively. Some interesting phenomena observed during the measurement process were explained. Additionally, optical and coupling feedforward systems were developed using quadrupole and skew quadrupole magnets installed on the front and back of elliptically polarizing undulators(EPUs). Moreover, over nearly four months of operation, the developed strategy delivered a satisfactory performance in the SSRF storage ring.