Functional constipation （FC） is a common functional bowel disorder disease that affects life quality of a large number of people. This study aimed to explore the impact of different intensities of electro-acupunctu...Functional constipation （FC） is a common functional bowel disorder disease that affects life quality of a large number of people. This study aimed to explore the impact of different intensities of electro-acupuncture （EA） treatment for FC patients. Totally, 111 patients with FC meeting the Rome III criteria were randomly assigned to different intensities of EA groups （low and high intensity of EA groups） and medicine-controlled （MC） group. In EA groups, patients were treated with EA at quchi （Llll） and shangjuxu （ST37） bilaterally for 4 weeks, 5 times/week in the first 2 weeks, and 3 times/week in the last 2 weeks. In MC group, 5 mg mosapride citrate was administered orally 3 times/day for 4 weeks. Spontaneous bowel movement frequency each day was recorded using a consti- pation diary. Self-rating anxiety scale （SAS） and self-rating depression scale （SDS） were used to assess the patients＇ psychological state. Cortisol （CORT）, substance P （SP）, and vasoactive intestinal polypep- tide （VIP） were evaluated at baseline and at the end of 4 weeks after treatment. As compared with the baseline, there was statistically significant increase in stool frequency every week （P〈0.01）, but there was no statistically significant difference among the three groups. As compared with the baseline, after 4 weeks of EA therapy, the scores of SDS and serum levels of CORT were decreased significantly in low intensity of EA group （P〈0.01）, and the serum levels of SP and VIP were increased significantly （P〈0.05）; the scores of SAS and SDS and serum levels of CORT were decreased significantly in high intensity of EA group （P〈0.05）, and the serum levels of SP and VIP were increased significantly （P〈0.05）; the serum levels of CORT and VIP were increased significantly in MC group （P〈0.05）. As compared with MC group, after 4 weeks of treatment, the serum levels of SP were signifcicantly in- creased in low intensity of EA group （P〈0.01）. Low and high intensities of EA could increase the stool frequency, improve the FC patient＇s anxiety and depression, reduce the serum levels of CORT, and in- crease the serum levels of SP and VIP effectively. It is concluded that both low and high intensities of EA are effective for FC patients, but there is no significant difference between the low and high intensi- ties of EA.展开更多
目的:观察电针天枢、上巨虚穴对结肠炎大鼠肠黏膜中维生素D受体(VDR)表达的影响,探讨电针治疗溃疡性结肠炎(UC)的可能机制。方法:按照随机数字表法将雄性Wistar大鼠分为对照组、模型组和电针组,每组8只。采用三硝基苯磺酸(TNBS)灌肠的...目的:观察电针天枢、上巨虚穴对结肠炎大鼠肠黏膜中维生素D受体(VDR)表达的影响,探讨电针治疗溃疡性结肠炎(UC)的可能机制。方法:按照随机数字表法将雄性Wistar大鼠分为对照组、模型组和电针组,每组8只。采用三硝基苯磺酸(TNBS)灌肠的方法来诱导溃疡性结肠炎制备大鼠模型,同时采用大鼠疾病活动指数(DAI)评分和组织学活动度评分(HAI)来评估结肠炎症程度。电针组大鼠经TNBS造模成功后次日取天枢、上巨虚穴进行电针治疗,共14 d。于第15天处死大鼠后收集结肠组织,分别采用免疫组化和实时荧光定量PCR法(qRT-PCR)检测肠黏膜VDR的表达水平,采用分光光度法测定结肠组织髓过氧化物酶(MPO)活性。结果:与模型组比较,电针组HAI明显下降(P=0.005)。与对照组比较,模型组和电针组结肠组织中MPO活性均明显增加。与模型组比较,电针组结肠组织中MPO活性下降(P<0.001)。与对照组比较,模型组、电针组大鼠肠黏膜中VDR mRNA及蛋白表达降低(P<0.01)。与模型组比较,电针组大鼠肠黏膜VDR m RNA及蛋白表达升高(P<0.05)。蛋白免疫组化提示VDR主要在上皮细胞中表达。VDR蛋白表达水平与MPO活性成负相关(P<0.05)。结论:电针天枢、上巨虚两穴可能通过上调肠黏膜VDR表达来缓解大鼠UC症状。展开更多
OBJECTIVE:To evaluate the effects of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors,and to explore an efficient and noninvas...OBJECTIVE:To evaluate the effects of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors,and to explore an efficient and noninvasive method for postoperative recovery of bowel functions.METHODS:Forty patients were randomly and evenly assigned into a regular nursing care group(RNC) and an acupoint electric stimulation group(AES).Patients in the RNC group received regular nursing care and patients in the AES group received regular nursing care plus electric stimulation of acupoints.The serum levels of gastrin(GAS),motilin(MOT),and cholecystokinin(CCK),and an electrogastrogram(EGG) of all the patients were evaluated on the first,third,and fifth day after surgery.The time to first flatus after surgery and the number of patients with side effects such as abdominal pain,abdominal distention,and diarrhea were recorded.RESULTS:There were significant differences between the two groups in GAS,MOT,EGG,time to first flatus,abdominal pain,abdominal distention,and diarrhea(P < 0.05).CONCLUSION:Electric stimulation on acupoints could increase levels of GAS and MOT,promote the recovery of gastrointestinal functions,and decrease complications among postoperative senile patients with gastrointestinal tumors.展开更多
Objective： To compare the effects between electroacupuncture （EA） at Chize （LU 5, the He-Sea point of the Lung Meridian） and Shangjuxu （ST 37, the lower He-Sea point of the large intestine） in rats with ulcerat...Objective： To compare the effects between electroacupuncture （EA） at Chize （LU 5, the He-Sea point of the Lung Meridian） and Shangjuxu （ST 37, the lower He-Sea point of the large intestine） in rats with ulcerative colitis （UC） on the variations of mesenteric microcirculation and vasoactive intestinal peptide （VIP） in the colon, lung, and hypothalamus. The relative specificity of acupoints was also explored. Methods： A total of 28 male Wistar rats were randomized into a normal group, a model group, a Chize （LU 5） group and a Shangjuxu （ST 37） group, 7 rats in each group. The UC model was established by enema with acetic acid. Since the third day after modeling, rats in the Chize （LU 5） group and Shangjuxu （ST 37） group respectively received EA at Chize （LU 5） and Shangjuxu （ST 37）, 1S min each time for successive 7 d. The variations of mesenteric microvascular calibers and blood flow status were observed by a microcirculation microscopic tester; VIP in the colon, lung and hypothalamus was measured by radioimmunoassay. Results： Compared with the normal group, the mesenteric microvascular calibers were significantly expanded in the model group （P〈0.05）; there was no significant difference between the model group and Chize （LU 5） group （P〉0.05）; compared with the model group and Chize （LU 5） group, the calibers were obviously shrunk in Shangjuxu （ST 37） group （P〈0.05）. The four groups showed no significant inter-group differences in comparing blood flow status （P〉0.05）. The colonic VIP levels in the model group and Chize （LU 5） group were significantly higher than that in the normal group （P〈0.01, P〈0.05）; the VIP level in Shangjuxu （ST 37） group was markedly lower than that in the model group （P〈0.01）. There were no significant differences among the four groups in comparing VIP level in lung and hypothalamus （P〉0.05）. Conclusion： The effects of Chize （LU 5） and Shangjuxu （ST 37） were different in treating UC. Shangjuxu （ST 37） showed a more significant efficacy in down-regulating VIP in the colon and regulating mesenteric microcirculation, while the effects of Chize （LU 5） were not obvious.展开更多
Objective： To probe into the mechanisms of electroacupuncture （EA） at Shangjuxu （ST 37） for treatment of the ulcerative colitis （UC）. Methods： Forty male Wistar rats were randomly divided into 4 groups： a nor...Objective： To probe into the mechanisms of electroacupuncture （EA） at Shangjuxu （ST 37） for treatment of the ulcerative colitis （UC）. Methods： Forty male Wistar rats were randomly divided into 4 groups： a normal group, a model group, a Shangjuxu group and a non-acupoint group, 10 rats in each group. The UC rat model was made with enema of trinitro-benzenesulfonic acid （TNBSA）, and the changes of interleuldn-1β （IL-1β） and interleukin-4 （IL-4） contents after EA at Shangjuxu （ST 37） were observed. Results： EA at Shangjuxu （ST 37） could significantly decrease the IL-1β content and increase the IL-4 content in the colic tissues of the UC rats with significant differences as compared with the model group and the non-acupoint group （P〈0.05 or P〈0.01）. Conclusion： The mechanisms of EA at Shangjuxu （ST 37） for treatment of the UC rats is possibly related with the decrease of IL-1β, a inflammation-promoting cytokine, and the increase of IL-4, a anti-inflammatory cytokine.展开更多
基金supported by the National Basic Research Program of China(973 Program)(No.2011CB505203)
文摘Functional constipation （FC） is a common functional bowel disorder disease that affects life quality of a large number of people. This study aimed to explore the impact of different intensities of electro-acupuncture （EA） treatment for FC patients. Totally, 111 patients with FC meeting the Rome III criteria were randomly assigned to different intensities of EA groups （low and high intensity of EA groups） and medicine-controlled （MC） group. In EA groups, patients were treated with EA at quchi （Llll） and shangjuxu （ST37） bilaterally for 4 weeks, 5 times/week in the first 2 weeks, and 3 times/week in the last 2 weeks. In MC group, 5 mg mosapride citrate was administered orally 3 times/day for 4 weeks. Spontaneous bowel movement frequency each day was recorded using a consti- pation diary. Self-rating anxiety scale （SAS） and self-rating depression scale （SDS） were used to assess the patients＇ psychological state. Cortisol （CORT）, substance P （SP）, and vasoactive intestinal polypep- tide （VIP） were evaluated at baseline and at the end of 4 weeks after treatment. As compared with the baseline, there was statistically significant increase in stool frequency every week （P〈0.01）, but there was no statistically significant difference among the three groups. As compared with the baseline, after 4 weeks of EA therapy, the scores of SDS and serum levels of CORT were decreased significantly in low intensity of EA group （P〈0.01）, and the serum levels of SP and VIP were increased significantly （P〈0.05）; the scores of SAS and SDS and serum levels of CORT were decreased significantly in high intensity of EA group （P〈0.05）, and the serum levels of SP and VIP were increased significantly （P〈0.05）; the serum levels of CORT and VIP were increased significantly in MC group （P〈0.05）. As compared with MC group, after 4 weeks of treatment, the serum levels of SP were signifcicantly in- creased in low intensity of EA group （P〈0.01）. Low and high intensities of EA could increase the stool frequency, improve the FC patient＇s anxiety and depression, reduce the serum levels of CORT, and in- crease the serum levels of SP and VIP effectively. It is concluded that both low and high intensities of EA are effective for FC patients, but there is no significant difference between the low and high intensi- ties of EA.
文摘目的:观察电针天枢、上巨虚穴对结肠炎大鼠肠黏膜中维生素D受体(VDR)表达的影响,探讨电针治疗溃疡性结肠炎(UC)的可能机制。方法:按照随机数字表法将雄性Wistar大鼠分为对照组、模型组和电针组,每组8只。采用三硝基苯磺酸(TNBS)灌肠的方法来诱导溃疡性结肠炎制备大鼠模型,同时采用大鼠疾病活动指数(DAI)评分和组织学活动度评分(HAI)来评估结肠炎症程度。电针组大鼠经TNBS造模成功后次日取天枢、上巨虚穴进行电针治疗,共14 d。于第15天处死大鼠后收集结肠组织,分别采用免疫组化和实时荧光定量PCR法(qRT-PCR)检测肠黏膜VDR的表达水平,采用分光光度法测定结肠组织髓过氧化物酶(MPO)活性。结果:与模型组比较,电针组HAI明显下降(P=0.005)。与对照组比较,模型组和电针组结肠组织中MPO活性均明显增加。与模型组比较,电针组结肠组织中MPO活性下降(P<0.001)。与对照组比较,模型组、电针组大鼠肠黏膜中VDR mRNA及蛋白表达降低(P<0.01)。与模型组比较,电针组大鼠肠黏膜VDR m RNA及蛋白表达升高(P<0.05)。蛋白免疫组化提示VDR主要在上皮细胞中表达。VDR蛋白表达水平与MPO活性成负相关(P<0.05)。结论:电针天枢、上巨虚两穴可能通过上调肠黏膜VDR表达来缓解大鼠UC症状。
基金Supported by the National Natural Science Foundation Project of China(Transcutaneous Electrical Acupoint Stimulation Prevents the Deep Vein Thrombosis:the Role of Endothelium Modulation,No.81202750)
文摘OBJECTIVE:To evaluate the effects of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors,and to explore an efficient and noninvasive method for postoperative recovery of bowel functions.METHODS:Forty patients were randomly and evenly assigned into a regular nursing care group(RNC) and an acupoint electric stimulation group(AES).Patients in the RNC group received regular nursing care and patients in the AES group received regular nursing care plus electric stimulation of acupoints.The serum levels of gastrin(GAS),motilin(MOT),and cholecystokinin(CCK),and an electrogastrogram(EGG) of all the patients were evaluated on the first,third,and fifth day after surgery.The time to first flatus after surgery and the number of patients with side effects such as abdominal pain,abdominal distention,and diarrhea were recorded.RESULTS:There were significant differences between the two groups in GAS,MOT,EGG,time to first flatus,abdominal pain,abdominal distention,and diarrhea(P < 0.05).CONCLUSION:Electric stimulation on acupoints could increase levels of GAS and MOT,promote the recovery of gastrointestinal functions,and decrease complications among postoperative senile patients with gastrointestinal tumors.
基金supported by the Independent Subject Project of Beijing University of Chinese Medicine for Postgraduate~~
文摘Objective： To compare the effects between electroacupuncture （EA） at Chize （LU 5, the He-Sea point of the Lung Meridian） and Shangjuxu （ST 37, the lower He-Sea point of the large intestine） in rats with ulcerative colitis （UC） on the variations of mesenteric microcirculation and vasoactive intestinal peptide （VIP） in the colon, lung, and hypothalamus. The relative specificity of acupoints was also explored. Methods： A total of 28 male Wistar rats were randomized into a normal group, a model group, a Chize （LU 5） group and a Shangjuxu （ST 37） group, 7 rats in each group. The UC model was established by enema with acetic acid. Since the third day after modeling, rats in the Chize （LU 5） group and Shangjuxu （ST 37） group respectively received EA at Chize （LU 5） and Shangjuxu （ST 37）, 1S min each time for successive 7 d. The variations of mesenteric microvascular calibers and blood flow status were observed by a microcirculation microscopic tester; VIP in the colon, lung and hypothalamus was measured by radioimmunoassay. Results： Compared with the normal group, the mesenteric microvascular calibers were significantly expanded in the model group （P〈0.05）; there was no significant difference between the model group and Chize （LU 5） group （P〉0.05）; compared with the model group and Chize （LU 5） group, the calibers were obviously shrunk in Shangjuxu （ST 37） group （P〈0.05）. The four groups showed no significant inter-group differences in comparing blood flow status （P〉0.05）. The colonic VIP levels in the model group and Chize （LU 5） group were significantly higher than that in the normal group （P〈0.01, P〈0.05）; the VIP level in Shangjuxu （ST 37） group was markedly lower than that in the model group （P〈0.01）. There were no significant differences among the four groups in comparing VIP level in lung and hypothalamus （P〉0.05）. Conclusion： The effects of Chize （LU 5） and Shangjuxu （ST 37） were different in treating UC. Shangjuxu （ST 37） showed a more significant efficacy in down-regulating VIP in the colon and regulating mesenteric microcirculation, while the effects of Chize （LU 5） were not obvious.
文摘Objective： To probe into the mechanisms of electroacupuncture （EA） at Shangjuxu （ST 37） for treatment of the ulcerative colitis （UC）. Methods： Forty male Wistar rats were randomly divided into 4 groups： a normal group, a model group, a Shangjuxu group and a non-acupoint group, 10 rats in each group. The UC rat model was made with enema of trinitro-benzenesulfonic acid （TNBSA）, and the changes of interleuldn-1β （IL-1β） and interleukin-4 （IL-4） contents after EA at Shangjuxu （ST 37） were observed. Results： EA at Shangjuxu （ST 37） could significantly decrease the IL-1β content and increase the IL-4 content in the colic tissues of the UC rats with significant differences as compared with the model group and the non-acupoint group （P〈0.05 or P〈0.01）. Conclusion： The mechanisms of EA at Shangjuxu （ST 37） for treatment of the UC rats is possibly related with the decrease of IL-1β, a inflammation-promoting cytokine, and the increase of IL-4, a anti-inflammatory cytokine.
