[Objectives]The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake,and to verify its whitening effect.[Methods]With T.matsutake as the research object,the effects of...[Objectives]The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake,and to verify its whitening effect.[Methods]With T.matsutake as the research object,the effects of eluent concentration,loading amount and diameter-to-height ratio on the refining process of T.matsutake polysaccharide were explored by orthogonal test,to optimize the dynamic elution conditions of T.matsutake polysaccharide.The survival rate of mouse melanoma cells(B16),inhibition of melanin synthesis and inhibition of tyrosinase activity of crude extract A1 and refined extracts A2-A4 were tested to verify the whitening effect of T.matsutake.[Results]The best extraction and refining process for T.matsutake polysaccharide was:eluent 40%ethanol(volume fraction),loading amount 30 mg/g(polysaccharide-resin),diameter-to-height ratio 1∶8.Under this condition,the extraction rate of T.matsutake can reach up to 33%(A3).The whitening efficacy test showed that crude extract A1 and refined extracts A2-A4 had good whitening effects,and the whitening effects were A3≥A4>A1>A2.[Conclusions]The study provides a reference for the application of T.matsutake polysaccharide in cosmetics.展开更多
Taking refined flour,matsutake powder,and highland barley powder as main raw materials,this experiment studied the optimal formula of matsutake highland barley biscuit. Besides,single factor experiment was carried out...Taking refined flour,matsutake powder,and highland barley powder as main raw materials,this experiment studied the optimal formula of matsutake highland barley biscuit. Besides,single factor experiment was carried out for the amount of highland barley powder,white granulated sugar,and shortening. Then,the response surface optimization analysis was made on crispness and sensory score of the biscuit. The experiment indicates that taking the refined flour as the base 100 g( 100%),the formula of 20% highland barley powder,25% white granulated sugar,and 26% shortening can bake crisp biscuit with complete shape,pure flavor and high quality.展开更多
A total of 10 Tricholoma matsutake strains isolated from Yajiang County, Sichuan Province of China were analyzed by using AFLP technique. The results showed that the genetic characteristics of Tricholoma matsutake str...A total of 10 Tricholoma matsutake strains isolated from Yajiang County, Sichuan Province of China were analyzed by using AFLP technique. The results showed that the genetic characteristics of Tricholoma matsutake strains can be properly revealed by AFLP, and among the 10 Tricholoma matsutake strains, the genetic aspects were very similar. At the level of 90% similarity, all the strains collected together, and at the similarity of 92 % , three AFLP groups formed. Simultaneously, the effects of quantity of the primers on the second AFLP PCR were determined. The optimal quantity of Eco +1/Mse+1, Eco + 2/Mse + 2 and Eco + 3/ Mse+3 primer pairs used in the AFLP was 1 pmol/5 pmol, 0. 6 pmol/10 pmol and 0.1 pmol/8 pmol, respectively.展开更多
A 5-year study on the soil ecological environment of matsutake (Trihcholoma Matsutake) growing areas near Changbai Mountains showed that several factors, such as climate, parent material, organism and landform, which ...A 5-year study on the soil ecological environment of matsutake (Trihcholoma Matsutake) growing areas near Changbai Mountains showed that several factors, such as climate, parent material, organism and landform, which form the soil ecosystem provided suitable ecological environment for the formation and growth of matsutake. How to improve the construction and function of the soil ecosystem in order to utilize the natural resource continuously was also studied.展开更多
Polysaccharides of Tricholoma matsutake Sing. were extracted with boilingwater, followed by precipitating with ethanol. Two membranes of different molecular weight cut-off, namely, 30 kD and 10 kD were chosen with ult...Polysaccharides of Tricholoma matsutake Sing. were extracted with boilingwater, followed by precipitating with ethanol. Two membranes of different molecular weight cut-off, namely, 30 kD and 10 kD were chosen with ultrafiltration. The antioxidant activity of the purification polysaccharides was evaluated by various antioxidant assay, including DPPH （l,l-diphenyl-2-picrylhydrazyl） radical scavenging, superoxide anion radical scavenging and hydrogen peroxide scavenging. The scavenging effect on hydroxyl radical and DPPH radical, which may be comparable to Vitamin C, while the scavenging effect on superoxide anion radicals was not obvious.展开更多
Evaluation for human body whitening effcacy of cosmetics with extract from Tricholoma matsutake Sing as main effective constituent was conducted. Thirty female volunteers with age between 20 and 45 years old were kind...Evaluation for human body whitening effcacy of cosmetics with extract from Tricholoma matsutake Sing as main effective constituent was conducted. Thirty female volunteers with age between 20 and 45 years old were kindly invited to use the cosmetic product on the inner side of their crank arm for a testing period of 8 weeks continuously. Values of MI, L＊ and ITA° of the skin were measured and the results display signifcant difference （ P〈0. 05） before and after the 8 weeks testing period. Duncan multiple comparisons showed that the whitening effcacy of cosmetic product formulated with the extract from Tricholoma matsutake Sing is signifcantly greater than that of the formulation matrix and equivalent with that of ascorbyl ethyl ether. Via the data collection and statistical analysis, this method can directly refect the whitening effect of active additives in cosmetics, and it is suitable for the evaluation of the whitening effcacy of cosmetics with additives.展开更多
【目的】提高松茸粗多糖提取率,探究松茸多糖纯化组分及抗氧化作用。【方法】采用超声波辅助水提醇沉法提取松茸粗多糖,优化提取工艺,使用DEAE-Sepharose Fast Flow离子柱分离纯化粗多糖,采用离子色谱法分析单糖成分,并测定体外抗氧化...【目的】提高松茸粗多糖提取率,探究松茸多糖纯化组分及抗氧化作用。【方法】采用超声波辅助水提醇沉法提取松茸粗多糖,优化提取工艺,使用DEAE-Sepharose Fast Flow离子柱分离纯化粗多糖,采用离子色谱法分析单糖成分,并测定体外抗氧化活性。【结果】最优提取工艺参数为:浸提时间2.25 h,料液比1∶31(g∶mL),浸提温度83.50℃,该条件下多糖提取率为8.67%。松茸粗多糖经分离纯化后收集到3种多糖组分(TMP-1、TMP-2、TMP-3),主要由岩藻糖、盐酸氨基葡萄糖、半乳糖、葡萄糖和甘露糖组成,其中TMP-1存在鼠李糖,TMP-2和TMP-3存在葡萄糖醛酸。松茸粗多糖和3种纯化多糖组分均具有一定的抗氧化活性且存在量效关系,抗氧化能力强弱为:松茸粗多糖>TMP-2>TMP-3>TMP-1。【结论】松茸多糖有一定的抗氧化活性,且松茸粗多糖效果最好,分离纯化后的多糖组分抗氧化活性有所降低。本研究为松茸多糖的开发利用提供了理论基础,对其功能食品研发具有重要意义。展开更多
The development and protection of Shangri-La wetlands in southwestern Yunnan Province is of great significance for the protection of regional biodiversity, ecotourism and regional development. To specify the functions...The development and protection of Shangri-La wetlands in southwestern Yunnan Province is of great significance for the protection of regional biodiversity, ecotourism and regional development. To specify the functions of Shangri-La wetlands, with the help of Analytic Hierarchy Process (AHP), 7 typical wetlands in Shangri-La (Bita Lake Wetland, Napa Lake Wetland, Shudu Lake Wetland, Dazhongdian Wetland, Xiaozhongdian Wetland, Dahei Lake Wetland, Jinsha River Wetland) were selected for the quantitative analysis of 20 indexes in 3 comprehensive assessment layers (service functions, protective functions and production functions). The results showed that Shangri-La wetlands followed the below sequence in function: Bita Lake Wetland (0.811 4), Shudu Lake Wetland (0.739 3), Napa Lake Wetland (0.695 3), Dahei Lake Wetland (0.659 5), Jinsha River Wetland (0.614 3), Dazhongdian Wetland (0.520 4) and Xiaozhongdian Wetland (0.505 2). As single indexes of those wetlands were compared, it was found that values of certain indexes varied greatly, and value differences among 5 of 20 indexes even reached more than 100%, among which that of water supply capacity and tourism recreational value (164.60%) remained the highest, then water supplementation capacity (121.17%) and habitat stability (109.05%), and others varied between 5.58%-91.10%. The assessment results were similar to actual conditions, thus such a method was proved scientific and practical.展开更多
文摘[Objectives]The paper was to determine the extraction and refining process of polysaccharide from Tricholoma matsutake,and to verify its whitening effect.[Methods]With T.matsutake as the research object,the effects of eluent concentration,loading amount and diameter-to-height ratio on the refining process of T.matsutake polysaccharide were explored by orthogonal test,to optimize the dynamic elution conditions of T.matsutake polysaccharide.The survival rate of mouse melanoma cells(B16),inhibition of melanin synthesis and inhibition of tyrosinase activity of crude extract A1 and refined extracts A2-A4 were tested to verify the whitening effect of T.matsutake.[Results]The best extraction and refining process for T.matsutake polysaccharide was:eluent 40%ethanol(volume fraction),loading amount 30 mg/g(polysaccharide-resin),diameter-to-height ratio 1∶8.Under this condition,the extraction rate of T.matsutake can reach up to 33%(A3).The whitening efficacy test showed that crude extract A1 and refined extracts A2-A4 had good whitening effects,and the whitening effects were A3≥A4>A1>A2.[Conclusions]The study provides a reference for the application of T.matsutake polysaccharide in cosmetics.
基金Supported by Agricultural Product Processing Technology and Product Development Project of the 13th Five-Year Plan of Tibet
文摘Taking refined flour,matsutake powder,and highland barley powder as main raw materials,this experiment studied the optimal formula of matsutake highland barley biscuit. Besides,single factor experiment was carried out for the amount of highland barley powder,white granulated sugar,and shortening. Then,the response surface optimization analysis was made on crispness and sensory score of the biscuit. The experiment indicates that taking the refined flour as the base 100 g( 100%),the formula of 20% highland barley powder,25% white granulated sugar,and 26% shortening can bake crisp biscuit with complete shape,pure flavor and high quality.
文摘A total of 10 Tricholoma matsutake strains isolated from Yajiang County, Sichuan Province of China were analyzed by using AFLP technique. The results showed that the genetic characteristics of Tricholoma matsutake strains can be properly revealed by AFLP, and among the 10 Tricholoma matsutake strains, the genetic aspects were very similar. At the level of 90% similarity, all the strains collected together, and at the similarity of 92 % , three AFLP groups formed. Simultaneously, the effects of quantity of the primers on the second AFLP PCR were determined. The optimal quantity of Eco +1/Mse+1, Eco + 2/Mse + 2 and Eco + 3/ Mse+3 primer pairs used in the AFLP was 1 pmol/5 pmol, 0. 6 pmol/10 pmol and 0.1 pmol/8 pmol, respectively.
文摘A 5-year study on the soil ecological environment of matsutake (Trihcholoma Matsutake) growing areas near Changbai Mountains showed that several factors, such as climate, parent material, organism and landform, which form the soil ecosystem provided suitable ecological environment for the formation and growth of matsutake. How to improve the construction and function of the soil ecosystem in order to utilize the natural resource continuously was also studied.
文摘Polysaccharides of Tricholoma matsutake Sing. were extracted with boilingwater, followed by precipitating with ethanol. Two membranes of different molecular weight cut-off, namely, 30 kD and 10 kD were chosen with ultrafiltration. The antioxidant activity of the purification polysaccharides was evaluated by various antioxidant assay, including DPPH （l,l-diphenyl-2-picrylhydrazyl） radical scavenging, superoxide anion radical scavenging and hydrogen peroxide scavenging. The scavenging effect on hydroxyl radical and DPPH radical, which may be comparable to Vitamin C, while the scavenging effect on superoxide anion radicals was not obvious.
文摘Evaluation for human body whitening effcacy of cosmetics with extract from Tricholoma matsutake Sing as main effective constituent was conducted. Thirty female volunteers with age between 20 and 45 years old were kindly invited to use the cosmetic product on the inner side of their crank arm for a testing period of 8 weeks continuously. Values of MI, L＊ and ITA° of the skin were measured and the results display signifcant difference （ P〈0. 05） before and after the 8 weeks testing period. Duncan multiple comparisons showed that the whitening effcacy of cosmetic product formulated with the extract from Tricholoma matsutake Sing is signifcantly greater than that of the formulation matrix and equivalent with that of ascorbyl ethyl ether. Via the data collection and statistical analysis, this method can directly refect the whitening effect of active additives in cosmetics, and it is suitable for the evaluation of the whitening effcacy of cosmetics with additives.
文摘【目的】提高松茸粗多糖提取率,探究松茸多糖纯化组分及抗氧化作用。【方法】采用超声波辅助水提醇沉法提取松茸粗多糖,优化提取工艺,使用DEAE-Sepharose Fast Flow离子柱分离纯化粗多糖,采用离子色谱法分析单糖成分,并测定体外抗氧化活性。【结果】最优提取工艺参数为:浸提时间2.25 h,料液比1∶31(g∶mL),浸提温度83.50℃,该条件下多糖提取率为8.67%。松茸粗多糖经分离纯化后收集到3种多糖组分(TMP-1、TMP-2、TMP-3),主要由岩藻糖、盐酸氨基葡萄糖、半乳糖、葡萄糖和甘露糖组成,其中TMP-1存在鼠李糖,TMP-2和TMP-3存在葡萄糖醛酸。松茸粗多糖和3种纯化多糖组分均具有一定的抗氧化活性且存在量效关系,抗氧化能力强弱为:松茸粗多糖>TMP-2>TMP-3>TMP-1。【结论】松茸多糖有一定的抗氧化活性,且松茸粗多糖效果最好,分离纯化后的多糖组分抗氧化活性有所降低。本研究为松茸多糖的开发利用提供了理论基础,对其功能食品研发具有重要意义。
基金Supported by National Natural Science Foundation of China(40861009)Provincial Natural Science Foundation of Yunnan(2002D0036M)~~
文摘The development and protection of Shangri-La wetlands in southwestern Yunnan Province is of great significance for the protection of regional biodiversity, ecotourism and regional development. To specify the functions of Shangri-La wetlands, with the help of Analytic Hierarchy Process (AHP), 7 typical wetlands in Shangri-La (Bita Lake Wetland, Napa Lake Wetland, Shudu Lake Wetland, Dazhongdian Wetland, Xiaozhongdian Wetland, Dahei Lake Wetland, Jinsha River Wetland) were selected for the quantitative analysis of 20 indexes in 3 comprehensive assessment layers (service functions, protective functions and production functions). The results showed that Shangri-La wetlands followed the below sequence in function: Bita Lake Wetland (0.811 4), Shudu Lake Wetland (0.739 3), Napa Lake Wetland (0.695 3), Dahei Lake Wetland (0.659 5), Jinsha River Wetland (0.614 3), Dazhongdian Wetland (0.520 4) and Xiaozhongdian Wetland (0.505 2). As single indexes of those wetlands were compared, it was found that values of certain indexes varied greatly, and value differences among 5 of 20 indexes even reached more than 100%, among which that of water supply capacity and tourism recreational value (164.60%) remained the highest, then water supplementation capacity (121.17%) and habitat stability (109.05%), and others varied between 5.58%-91.10%. The assessment results were similar to actual conditions, thus such a method was proved scientific and practical.
