Autism spectrum disorders(ASD)are characterized by social and repetitive abnormalities.Although the ASD mouse model with Shank3b mutations is widely used in ASD research,the behavioral phenotype of this model has not ...Autism spectrum disorders(ASD)are characterized by social and repetitive abnormalities.Although the ASD mouse model with Shank3b mutations is widely used in ASD research,the behavioral phenotype of this model has not been fully elucidated.Here,a 3D-motion capture system and linear discriminant analysis were used to comprehensively record and analyze the behavioral patterns of male and female Shank3b mutant mice.It was found that both sexes replicated the core and accompanied symptoms of ASD,with significant sex differences.Further,Shank3b heterozygous knockout mice exhibited distinct autistic behaviors,that were significantly different from those those observed in the wild type and homozygous knockout groups.Our findings provide evidence for the inclusion of both sexes and experimental approaches to efficiently characterize heterozygous transgenic models,which are more clinically relevant in autistic studies.展开更多
基金National Science and Technology Innovation 2030-Major Project of China(2021ZD0201003 and 2022ZD0208300)National Natural Science Foundation of China(32171092)+3 种基金Shenzhen Key Basic Research Project(JCYJ20220818100805013)Shenzhen Key Laboratory of Viral Vectors for Biomedicine(ZDSYS20200811142401005)Guangdong Provincial Medical Products Administration Key Laboratory of Quality Control Technology for Virus-Based Therapeutics(2020ZDB26)Guangdong Provincial Medical Products Administration(2022ZDZ13).
文摘Autism spectrum disorders(ASD)are characterized by social and repetitive abnormalities.Although the ASD mouse model with Shank3b mutations is widely used in ASD research,the behavioral phenotype of this model has not been fully elucidated.Here,a 3D-motion capture system and linear discriminant analysis were used to comprehensively record and analyze the behavioral patterns of male and female Shank3b mutant mice.It was found that both sexes replicated the core and accompanied symptoms of ASD,with significant sex differences.Further,Shank3b heterozygous knockout mice exhibited distinct autistic behaviors,that were significantly different from those those observed in the wild type and homozygous knockout groups.Our findings provide evidence for the inclusion of both sexes and experimental approaches to efficiently characterize heterozygous transgenic models,which are more clinically relevant in autistic studies.
