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Thinning intensity affects microbial functional diversity and enzymatic activities associated with litter decomposition in a Chinese fir plantation 被引量:7
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作者 Wenya Xiao Fei Fei +2 位作者 Jiaojiao Diao Bin J.W.Chen Qingwei Guan 《Journal of Forestry Research》 SCIE CAS CSCD 2018年第5期1337-1350,共14页
Microbial functional diversity and enzymatic activities are critical to maintaining material circulation during litter decomposition in forests.Thinning,an important and widely used silvicultural treatment,changes the... Microbial functional diversity and enzymatic activities are critical to maintaining material circulation during litter decomposition in forests.Thinning,an important and widely used silvicultural treatment,changes the microclimate and promotes forest renewal.However,how thinning affects microbial functional diversity and enzymatic activities during litter decomposition remains poorly understood.We conducted thinning treatments in a Chinese fir plantation in a subtropical region of China with four levels of tree stem removal(0,30,50,and 70%),each with three replicates,and the effects of thinning on microbial functional diversity and enzymatic activities were studied 7 years after treatment by collecting litter samples four times over a 1-year period.Microbial functional diversity and enzymatic activities were analyzed using Biolog Ecoplates(Biolog Inc.,Hayward,CA,USA)based on the utilization of 31 carbon substrates.Total microbial abundance during litter decomposition was lower after the thinning treatments than without thinning.Microbial functional diversity did not differ significantly during litter decomposition,but the types of microbial carbon-source utilization did differ significantly with the thinning treatments.Microbial cellulase and invertase activities during litter decomposition were significantly higher under the thinning treatments due to changes in the litter carbon concentration and the ratios of carbon and lignin to nitrogen.The present study demonstrated the important influence of thinning on microbial activities during litter decomposition.Moderate-intensity thinning may maximize vegetation diversity and,in turn,increase the available substrate sources for microbial organisms in litter and promote nutrient cycling in forest ecosystems. 展开更多
关键词 Biology technology Litter decomposition Microbial enzymatic activities shannon diversity index
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Arbuscular mycorrhizal fungal diversity in some commonly occurring medicinal plants of Western Ghats,Goa region 被引量:3
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作者 K.P.Radhika B.F.Rodrigues 《Journal of Forestry Research》 SCIE CAS CSCD 2010年第1期45-52,I0002,共9页
Thirty-six medicinal plant species belonging to 25 families were surveyed to study Arbuscular mycorrhizal (AM) fungal diversity from different localities of North and South Goa of Western Ghats, Goa region, India. A... Thirty-six medicinal plant species belonging to 25 families were surveyed to study Arbuscular mycorrhizal (AM) fungal diversity from different localities of North and South Goa of Western Ghats, Goa region, India. A total of 30 medicinal plant species were found to be mycorrhizal and six plant species showed absence of AM fungal colonization. Forty two AM fungal species belonging to five genera viz., Glomus, Acaulospora, Scutellospora, Gigaspora and Ambispora were recovered from the rhizosphere soil. Glomus was found to be the most dominant genera in the study sites, and Glomus fasciculatum the most dominant AM fungal species. Negative significant correlation was observed between percent colonization and spore density. Simpson's and Shannon Weiner diversity index studies carried out in North and South Goa exhibited less variation in both the sites, indicating a stable and a diverse plant community. 展开更多
关键词 Arbuscular mycorrhizal fungi Glomus medicinal plants Western Ghats shannon Weiner diversity index spore density
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Information Entropy Measures for Stand Structural Diversity:Joint Entropy 被引量:2
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作者 LeiXiangdong LuYuanchang 《Forestry Studies in China》 CAS 2004年第2期12-15,共4页
Structural diversity is the key attribute of a stand. A set of biodiversity measures in ecology was introduced in forest management for describing stand structure, of which Shannon information entropy (Shannon index) ... Structural diversity is the key attribute of a stand. A set of biodiversity measures in ecology was introduced in forest management for describing stand structure, of which Shannon information entropy (Shannon index) has been the most widely used measure of species diversity. It is generally thought that tree size diversity could serve as a good proxy for height diversity. However, tree size diversity and height diversity for stand structure is not completely consistent. Stand diameter cannot reflect height information completely. Either tree size diversity or height diversity is one-dimensional information entropy measure. This paper discussed the method of multiple-dimensional information entropy measure with the concept of joint entropy. It is suggested that joint entropy is a good measure for describing overall stand structural diversity. 展开更多
关键词 stand structural diversity shannon index joint entropy conditional entropy
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Spatial pattern of tree diversity and evenness across forest types in Majella National Park,Italy 被引量:1
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作者 Mohammad Redowan 《Forest Ecosystems》 SCIE CSCD 2015年第4期304-313,共10页
Background: Estimation of tree diversity at broader scale is important for conservation planning. Tree diversity should be measured and understood in terms of diversity and evenness, two integral components to descri... Background: Estimation of tree diversity at broader scale is important for conservation planning. Tree diversity should be measured and understood in terms of diversity and evenness, two integral components to describe the structure of a biological community. Variation of the tree diversity and evenness with elevation, topographic relief, aspect, terrain shape, slope, soil nutrient, solar radiation etc. are well documented. Methods: Present study explores the variation of tree diversity (measured as Shannon diversity and evenness indices) of Majella National Park, italy with five available forest types namely evergreen oak woods, deciduous oak woods, blacWaleppo pine stands, hop-hornbeam forest and beech forest, using satellite, environmental and field data. Results: Hop-hornbeam forest was found to be most diverse and even while evergreen Oak woods was the lowest diverse and even. Diversity and evenness of forest types were concurrent to each other i.e. forest type which was more diverse was also more even. As a broad pattern, majority portion of the study area belonged to medium diversity and high evenness class. Conclusions: Satellite images and other GIS data proved useful tools in monitoring variation of tree diversity and evenness across various forest types. Present study findings may have implications in prioritizing conservation zones of high tree diversity at Majella. 展开更多
关键词 Tree diversity Tree evenness Forest type shannon diversity index shannon evenness index Neural network Kappa statistic
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Relationship between diversity of forest plant species and environmental gradient in eastern mountainous area of Heilongjiang Province, China 被引量:2
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作者 WANG Qing-gui XING Ya-juan +1 位作者 ZHOU Xiao-feng HAN Shi-jie 《Journal of Forestry Research》 SCIE CAS CSCD 2006年第3期252-254,共3页
Twenty-three secondary forest communities with different structure were selected in Mao'er Mountain National Park of Heilongjiang Province, China to study the relationship between diversity of forest plant species an... Twenty-three secondary forest communities with different structure were selected in Mao'er Mountain National Park of Heilongjiang Province, China to study the relationship between diversity of forest plant species and environmental gradient. The forest plant species diversity was analyzed by the diversity index, and the environmental factors was quantified by the method of Whittaker's quantification of environmental gradient. Meanwhile, β-diversity indexes of communities were calculated with similar measurements. The results showed that the Shannon-wiener diversity index of forest plant species increased with the increase of the environmental gradient, and the β-diversity indexes of communities showed a liner increase along with the change of environmental gradient. 展开更多
关键词 species diversity Forest plant species Heilongjiang Province shannon-wiener diversity β-diversity index Environmental gradient
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Feedback Between Influent Condition Heterogeneity and Diversity of Bacterial Community in Bioreactor at Low Substrate Concentration
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作者 Hui Feng Wei Wang +3 位作者 Zhiwei Zhao Maoan Du Fuyi Cui Ruisha Shi 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2017年第6期60-66,共7页
In order to investigate the effect of influent condition heterogeneity on diversity of the bacterial community,the degree of microbial resolution and effluent quality,biological treatment of micro-polluted source wate... In order to investigate the effect of influent condition heterogeneity on diversity of the bacterial community,the degree of microbial resolution and effluent quality,biological treatment of micro-polluted source water is proposed. Scanning Electron Microscopy( SEM) analysis reflects that influent conditions change the morphologies of biofilm. Denaturing Gradient Gel Electrophoresis( DGGE) analysis shows differences of H values are due to succession of functional bacterial communities. Microbial resolution values and species identifications reveal organic carbon is the main cause of community differentiation and bacterial migration. 展开更多
关键词 BACTERIAL COMMUNITY diversity BIOREACTOR cluster analysis INFLUENT HETEROGENEITY shannon index
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Bayesian Estimation of Shrubs Diversity in Rangelands under Two Management Systems in Northern Syria
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作者 Abdoul Aziz Niane Murari Singh Paul C. Struik 《Open Journal of Ecology》 2014年第4期168-173,共6页
The diversity of shrubs in rangelands of northern Syria is affected by the grazing management systems restricted by the increase in human and livestock populations. To describe and estimate diversity and compare the r... The diversity of shrubs in rangelands of northern Syria is affected by the grazing management systems restricted by the increase in human and livestock populations. To describe and estimate diversity and compare the rangeland grazing management treatments, two popular indices for diversity, the Shannon index and the Simpson index, were studied for the four combinations of two sites, Hammam and Obeisan, and two grazing methods, Closed and Open, using frequentist and Bayesian approaches. We simulated the a priori and a-posteriori distributions of the Shannon and Simpson diversity indices, where from a range of values for a constant in the a priori distribution the best value normalizing the distribution of the diversity indices was chosen. The Bayesian diversity estimates were higher than their frequentist counterparts and had lower standard errors. The grazing methods at each site and sites under each grazing method delivered significant diversity of shrub species. The Bayesian estimates resulted in lower p-values than the frequentist approach for two cases reflecting in Bayesian method’s higher power. Bayesian approach is recommended as it has a wider framework for inference on diversity studies. 展开更多
关键词 Arid RANGELANDs species Abundance diversity shannon index sIMPsON diversity index Bayesian Method
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Quantifyingα-diversity as a continuous function of location——a case study of a temperate forest
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作者 Peijian Shi Brady K.Quinn +2 位作者 Long Chen Jie Gao Julian Schrader 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第6期1683-1691,共9页
α-diversity describes species diversity at local scales.The Simpson’s and Shannon-Wiener indices are widely used to characterizeα-diversity based on species abundances within a fixed study site(e.g.,a quadrat or pl... α-diversity describes species diversity at local scales.The Simpson’s and Shannon-Wiener indices are widely used to characterizeα-diversity based on species abundances within a fixed study site(e.g.,a quadrat or plot).Although such indices provide overall diversity estimates that can be analyzed,their values are not spatially continuous nor applicable in theory to any point within the study region,and thus they cannot be treated as spatial covariates for analyses of other variables.Herein,we extended the Simpson’s and Shannon-Wiener indices to create point estimates ofα-diversity for any location based on spatially explicit species occurrences within different bandwidths(i.e.,radii,with the location of interest as the center).For an arbitrary point in the study region,species occurrences within the circle plotting the bandwidth were weighted according to their distance from the center using a tri-cube kernel function,with occurrences closer to the center having greater weight than more distant ones.These novel kernel-basedα-diversity indices were tested using a tree dataset from a 400 m×400 m study region comprising a 200 m×200 m core region surrounded by a 100-m width buffer zone.Our newly extendedα-diversity indices did not disagree qualitatively with the traditional indices,and the former were slightly lower than the latter by<2%at medium and large band widths.The present work demonstrates the feasibility of using kernel-basedα-diversity indices to estimate diversity at any location in the study region and allows them to be used as quantifiable spatial covariates or predictors for other dependent variables of interest in future ecological studies.Spatially continuousα-diversity indices are useful to compare and monitor species trends in space and time,which is valuable for conservation practitioners. 展开更多
关键词 Distance-based weighting diversity continuum Kernel function shannon-Wiener index simpson’s index Tri-cube kernel
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Crop Diversity Measurement at Dufuya Gardens and Insukamini Irrigation Scheme in Lower Gweru Communal Lands
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作者 Matsa Mark 《Journal of Environmental Science and Engineering(B)》 2012年第5期673-681,共9页
Dufuya wetland gardens and Insukamini irrigation scheme in Lower Gweru communal areas of Gweru district are two examples of how rural communities can be self-reliant if they are capacitated. This study sought to compa... Dufuya wetland gardens and Insukamini irrigation scheme in Lower Gweru communal areas of Gweru district are two examples of how rural communities can be self-reliant if they are capacitated. This study sought to compare crop diversity between these two vegetable producing schemes. The location of the two areas and the selected plots were determined using the GPS (global positioning system) hand receiver. Maps of the quadrants were produced in a GIS (geographic information system) on a computer. Simpson's index D = sum (pi2) was used to measure crop diversity while the Shannon-Weiner index (/4) was used to measure crop evenness. Results show that although both schemes are viable, Dufuya Gardens have higher crop diversity than Insukamini irrigation scheme. The study recommends that AGRITEX officers at Insukamini must advise farmers to diversify their crops in order for their produce to be more competitive on the market and also to cushion farmers against the effects of natural hazards. Given the success of the two schemes, the government must seriously consider sustainable utilization of the many small-scale dams and wetlands lying idle across the country for crop production in order to empower poor rural communities as well as alleviate poverty. 展开更多
关键词 Crop diversity EVENNEss sirnpson's index shannon-Weiner index.
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Shannon-Wiener多样性指数两种计算方法的比较研究 被引量:50
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作者 王晶 焦燕 +3 位作者 任一平 薛莹 纪毓鹏 徐宾铎 《水产学报》 CAS CSCD 北大核心 2015年第8期1257-1263,共7页
在应用群落多样性指数评价调查海域群落物种多样性或进行海域生态环境评价时,常用基于样方数据(方法 1)或基于区域混合样数据(方法 2)2种方法计算Shannon-Wiener多样性指数。为了比较哪种方法所得结果能更好地反映调查海域群落多样性状... 在应用群落多样性指数评价调查海域群落物种多样性或进行海域生态环境评价时,常用基于样方数据(方法 1)或基于区域混合样数据(方法 2)2种方法计算Shannon-Wiener多样性指数。为了比较哪种方法所得结果能更好地反映调查海域群落多样性状况,并且分析多样性指数与样本含量的关系,本实验根据2009年胶州湾潮滩湿地大型底栖动物季度调查数据,应用再抽样模拟方法对2种多样性指数计算方法进行了比较研究。结果表明,对于相同样本含量,基于样方数据计算的Shannon-Wiener多样性指数值小于基于区域混合样数据计算的结果。方法 1所计算的群落多样性指数与样本含量无关;而方法 2计算的多样性指数值随样本含量的增加而增加,并在样本含量为20时接近"真值"并趋于稳定,其相对估计误差(REE)和相对偏差(RB)较小,表明样本含量影响方法 2的多样性指数的计算。在评价调查海域群落物种多样性或进行海域生态环境评价时,应该使用基于区域混合样数据的计算方法,并保证足够的样本含量,才能客观地反映海域群落生物多样性现状。 展开更多
关键词 shannon-Wiener多样性指数 计算方法 再抽样 最优样本含量
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基于Shannon-Wiener指数的中国森林物种多样性保育价值评估方法 被引量:123
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作者 王兵 郑秋红 郭浩 《林业科学研究》 CSCD 北大核心 2008年第2期268-274,共7页
针对森林物种多样性保育价值评估尚缺乏逻辑推理方法的现状,提出了更为客观合理的Shannon-Wiener指数评估法。此方法通过森林生态系统的Shannon-Wiener多样性指数和林分面积,计算其物种多样性保育价值。基于此方法,利用第六次全国森林... 针对森林物种多样性保育价值评估尚缺乏逻辑推理方法的现状,提出了更为客观合理的Shannon-Wiener指数评估法。此方法通过森林生态系统的Shannon-Wiener多样性指数和林分面积,计算其物种多样性保育价值。基于此方法,利用第六次全国森林资源连续清查数据和来自中国森林生态系统定位研究网络(CFERN)所属生态站的数万个观测数据,对中国森林物种多样性保育价值进行了计算。结果表明,黑龙江和云南两省生物物种保育总价值在全国名列前茅,海南省尽管单价最高,但森林面积较小,其总价值较低。广大西北以及华北地区森林平均单位面积价值和总价值普遍较低。将全国39种林分类型归为如下几类:单价高总量少型、单价高总量大型、单价低总量少型、单价低总量大型和中间类型,针对不同的类群采取不同的保护政策,有利于推动生物多样性保护工作。与目前流行的支付意愿法(WTP)相比,Shannon-Wiener指数评估法的优越性在于:以生态系统的自然属性为基础,每个指标都具有明确的意义,评价结果更接近理论认识,且结果之间可比性强。 展开更多
关键词 shannon-WIENER指数 森林物种多样性保育价值 中国
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电针对慢性应激抑郁大鼠行为学和肠道微生物每孔颜色平均变化率、Shannon-Wiener多样性指数的影响 被引量:9
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作者 宋蕙杉 王顺 《世界中西医结合杂志》 2020年第1期88-91,共4页
目的观察电针对慢性应激抑郁大鼠行为学和肠道菌群每孔颜色平均变化率(Average well color development,AWCD)、Shannon-Wiener多样性指数的影响,探讨电针治疗抑郁症的作用机制。方法将60只SD大鼠随机分为正常组、模型组、调神畅情组和... 目的观察电针对慢性应激抑郁大鼠行为学和肠道菌群每孔颜色平均变化率(Average well color development,AWCD)、Shannon-Wiener多样性指数的影响,探讨电针治疗抑郁症的作用机制。方法将60只SD大鼠随机分为正常组、模型组、调神畅情组和普通电针组,建立温和不可预见性应激抑郁模型,造模成功后予不同干预方法,观察各组大鼠行为学及肠道微生物AWCD、Shannon-Wiener多样性指数的变化。结果与正常组比较,模型组大鼠呈抑郁样表现,AWCD、Shannon-Wiener多样性指数均显著降低。与模型组比较,调神畅情组和普通电针组大鼠抑郁样症状明显改善,AWCD、Shannon-Wiener多样性指数均明显升高。结论电针能够改善慢性应激抑郁大鼠行为学表现,并提高其肠道微生物群落AWCD和Shannon-Wiener多样性指数,这可能是电针治疗抑郁症的作用机制之一。 展开更多
关键词 电针 AWCD shannon-Wiener多样性指数 抑郁
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基于Shannon-Wiener指数的三明市森林生物多样性评价 被引量:3
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作者 黄伙发 《福建林业科技》 2015年第4期121-124,共4页
参考LY/T 1721—2008森林生态系统服务功能评估规范,以2006年三明市森林资源二类调查数据为基础,采用Shannon-Wiener指数计算三明市森林生物多样性保护价值。结果表明:三明市森林生物多样性保护价值为396.80亿元,单位森林面积生物多样... 参考LY/T 1721—2008森林生态系统服务功能评估规范,以2006年三明市森林资源二类调查数据为基础,采用Shannon-Wiener指数计算三明市森林生物多样性保护价值。结果表明:三明市森林生物多样性保护价值为396.80亿元,单位森林面积生物多样性保护价值为2.28万元·hm-2,森林生物多样性保护价值占到福建省的20.58%,单位森林面积生物多样性保护价值略高于福建省平均水平(2.11万元·hm-2),高于全国平均水平(1.40万元·hm-2)。 展开更多
关键词 森林生物多样性 shannon-WIENER指数 保护价值
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Association between childhood obesity and gut microbiota:16S rRNA gene sequencing-based cohort study 被引量:1
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作者 Xu-Ming Li Qing Lv +2 位作者 Ya-Jun Chen Lu-Biao Yan Xin Xiong 《World Journal of Gastroenterology》 SCIE CAS 2024年第16期2249-2257,共9页
BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children(8-12 years old)using 16S rDNA sequencing.The research aimed to provide insights for mechanistic studies and prevent... BACKGROUND This study aimed to identify characteristic gut genera in obese and normal-weight children(8-12 years old)using 16S rDNA sequencing.The research aimed to provide insights for mechanistic studies and prevention strategies for childhood obesity.Thirty normal-weight and thirty age-and sex-matched obese children were included.Questionnaires and body measurements were collected,and fecal samples underwent 16S rDNA sequencing.Significant differences in body mass index(BMI)and body-fat percentage were observed between the groups.Analysis of gut microbiota diversity revealed lowerα-diversity in obese children.Differences in gut microbiota composition were found between the two groups.Prevotella and Firmicutes were more abundant in the obese group,while Bacteroides and Sanguibacteroides were more prevalent in the control group.AIM To identify the characteristic gut genera in obese and normal-weight children(8-12-year-old)using 16S rDNA sequencing,and provide a basis for subsequent mechanistic studies and prevention strategies for childhood obesity.METHODS Thirty each normal-weight,1:1 matched for age and sex,and obese children,with an obese status from 2020 to 2022,were included in the control and obese groups,respectively.Basic information was collected through questionnaires and body measurements were obtained from both obese and normal-weight children.Fecal samples were collected from both groups and subjected to 16S rDNA sequencing using an Illumina MiSeq sequencing platform for gut microbiota diversity analysis.RESULTS Significant differences in BMI and body-fat percentage were observed between the two groups.The Ace and Chao1 indices were significantly lower in the obese group than those in the control group,whereas differences were not significant in the Shannon and Simpson indices.Kruskal-Wallis tests indicated significant differences in unweighted and weighted UniFrac distances between the gut microbiota of normal-weight and obese children(P<0.01),suggesting substantial disparities in both the species and quantity of gut microbiota between the two groups.Prevotella,Firmicutes,Bacteroides,and Sanguibacteroides were more abundant in the obese and control groups,respectively.Heatmap results demonstrated significant differences in the gut microbiota composition between obese and normal-weight children.CONCLUSION Obese children exhibited lowerα-diversity in their gut microbiota than did the normal-weight children.Significant differences were observed in the composition of gut microbiota between obese and normal-weight children. 展开更多
关键词 Childhood obesity Gut microbiota 16s rDNA sequencing diversity analysis Genus identification Body mass index
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内蒙古小花棘豆遗传多样性的ISSR分析 被引量:19
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作者 卢萍 赵萌莉 +2 位作者 韩国栋 曹雪松 张丽 《西北植物学报》 CAS CSCD 北大核心 2007年第6期1102-1107,共6页
应用ISSR分子标记对内蒙古西部地区小花棘豆(Oxytropis glabra DC.)6个地理种群进行了遗传多样性研究.结果表明:小花棘豆具有较高的遗传多样性;14个引物共扩增出327个位点,物种水平上Nei's基因多样性指数为0.1972,Shannon多样性指数... 应用ISSR分子标记对内蒙古西部地区小花棘豆(Oxytropis glabra DC.)6个地理种群进行了遗传多样性研究.结果表明:小花棘豆具有较高的遗传多样性;14个引物共扩增出327个位点,物种水平上Nei's基因多样性指数为0.1972,Shannon多样性指数为0.3287;种内总基因多样性为0.1976,种群内基因多样性为0.1653,83.67%的遗传变异存在于种群内,16.33%的遗传变异存在于种群间,种群间的遗传分化系数为0.1633,基因流为2.5613,6个种群间有一定遗传分化,但遗传漂变不会引起遗传分化.UPGMA遗传距离聚类结果表明,生态地理条件相似的种群优先聚集. 展开更多
关键词 小花棘豆 IssR 遗传多样性 PCR Nei指数 shannon指数
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内蒙古扁蓿豆遗传多样性的ISSR分析 被引量:9
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作者 李鸿雁 李志勇 +2 位作者 王小丽 师文贵 邢建军 《西北植物学报》 CAS CSCD 北大核心 2011年第1期52-56,共5页
应用ISSR分子标记对分布于内蒙古不同地区的扁蓿豆(Medicago ruthenica)8个地理种群进行了遗传多样性研究.结果表明:扁蓿豆具有较高的遗传多样性;15个引物共扩增出363个位点,物种水平上Nei s基因多样性指数为0.198 9,Shannon多样性指数... 应用ISSR分子标记对分布于内蒙古不同地区的扁蓿豆(Medicago ruthenica)8个地理种群进行了遗传多样性研究.结果表明:扁蓿豆具有较高的遗传多样性;15个引物共扩增出363个位点,物种水平上Nei s基因多样性指数为0.198 9,Shannon多样性指数为0.303 7;种内总基因多样性为0.308 6,种群内基因多样性为0.198 9,64.45%的遗传变异存在于种群内,35.55%的遗传变异存在于种群间,种群间的遗传分化系数为0.355 5,基因流为0.906 4,8个种群间基因交流较少,遗传分化较强.UPGMA遗传距离聚类结果表明,生态地理条件相似的种群优先聚集. 展开更多
关键词 扁蓿豆 IssR 遗传多样性 Neis指数 shannon指数
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内蒙古西部地区砂珍棘豆(Oxytropis racemosa Turcz.)遗传多样性分析 被引量:5
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作者 卢萍 赵萌莉 +3 位作者 韩国栋 曹珍 陈曦 王中南 《内蒙古大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第2期160-165,共6页
用ISSR分子标记对内蒙古西部地区砂珍棘豆(Oxytropis racemosa Turcz)3个不同生境的地理种群进行了种群遗传多样性分析.结果表明砂珍棘豆种群具有较高的遗传多样性,14个ISSR引物扩增出284条带,Shannon多样性指数I=0.3630,Nei′s多样性指... 用ISSR分子标记对内蒙古西部地区砂珍棘豆(Oxytropis racemosa Turcz)3个不同生境的地理种群进行了种群遗传多样性分析.结果表明砂珍棘豆种群具有较高的遗传多样性,14个ISSR引物扩增出284条带,Shannon多样性指数I=0.3630,Nei′s多样性指数H=0.2234,物种总基因多样性(HT)为0.2236,种群内基因多样性(Hs)为0.2085,大部分遗传变异(93.27%)存在于种群内,6.73%的遗传变异存在于种群间.遗传分化系数(Gst)为0.0673,基因流(Nm)为6.9318,说明种群间有很强的基因交流,遗传漂变不会引起遗传分化.UPGMA遗传距离聚类结果表明,3个地理种群中,乌审旗和伊金霍洛种群先聚在一起,然后与鄂托克种群聚在一起,遗传距离与地理距离相关, 展开更多
关键词 砂珍棘豆 IssR 遗传多样性 Nei指数 shannon指数
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PCR-SSCP技术用于脱臭微生物群落结构的研究 被引量:13
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作者 米文秀 谢冰 徐亚同 《环境科学》 EI CAS CSCD 北大核心 2008年第7期1992-1997,共6页
采用PCR-SSCP(单链构象多态性)技术对脱臭生物滤池中填料生物膜微生物群落结构进行了研究.生物滤池对臭气中的污染物随驯化时间逐渐增强,去除率从50%升高到89%.对2种填料树皮和秸秆上生物膜的分析结果表明,滤池内微生物多样性随运行... 采用PCR-SSCP(单链构象多态性)技术对脱臭生物滤池中填料生物膜微生物群落结构进行了研究.生物滤池对臭气中的污染物随驯化时间逐渐增强,去除率从50%升高到89%.对2种填料树皮和秸秆上生物膜的分析结果表明,滤池内微生物多样性随运行时间先降后升,从1.6-1.9上升到2以上;而2种填料上微生物的相似性逐渐增加,表明随着驯化进程,生物填料上的微生物能够利用臭气污染物进行生长,并随运行时间逐渐趋于丰富和稳定;树皮比秸秆具有较高的生物多样性,平均值分别为2.2和2.0,说明树皮上容易附着多种微生物生长,电镜照片也显示2种填料生物膜的增长和生物多样性的提高.SSCP条带测序结果表明生物滤池生物膜优势微生物种属为芽孢杆菌属,占33.3%;条带中不可培养细菌占44.4%;填料生物膜上的细菌绝大多数是广泛存在于土壤、水体及生物体中的微生物,它们对环境适应能力强,在臭气的污染物去除中扮演重要角色. 展开更多
关键词 PCR-ssCP 生物除臭 生物膜 微生物群落结构 shannon-Wiener多样性指数
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狼尾草属优质牧草SRAP遗传多样性分析与指纹图谱构建 被引量:8
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作者 石秀兰 陈平 +2 位作者 于得水 韩瑞宏 刘萍 《广东农业科学》 CAS 2018年第10期55-60,共6页
为探讨狼尾草属优质牧草的遗传多样性,利用SRAP分子标记方法,对13份狼尾草属优质牧草种质进行遗传多样性分析,并构建了DNA指纹图谱,结果表明:(1)从72对SRAP引物中筛选出16对引物进行PCR扩增,共扩增出278条带,其中有218条具有多态性,平... 为探讨狼尾草属优质牧草的遗传多样性,利用SRAP分子标记方法,对13份狼尾草属优质牧草种质进行遗传多样性分析,并构建了DNA指纹图谱,结果表明:(1)从72对SRAP引物中筛选出16对引物进行PCR扩增,共扩增出278条带,其中有218条具有多态性,平均多态率为78.42%。(2)Shannon信息指数(I)和Nei's基因多样性指数(H)平均值分别为0.5017、0.3306,说明13份供试品种(系)具有较为丰富的遗传多样性。(3)利用UPGMA构建13份狼尾草种质资源的聚类图,在遗传相似系数为0.86处可将13份狼尾草品种(系)分为5类。(4)13个狼尾草品种(系)间存在较大的遗传差异,遗传距离在0.0206~0.9740之间。(5)利用1对引物扩增的17个多态性位点构建了狼尾草13个品种(系)的DNA指纹图谱,能有效区分13个品种(系)。 展开更多
关键词 狼尾草种质资源 sRAP 遗传多样性 shannon信息指数 DNA指纹图谱 聚类分析
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内蒙古地区刺叶柄棘豆(Oxytropis aciphylla Ledeb.)种群遗传多样性分析 被引量:4
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作者 卢萍 赵萌莉 +3 位作者 韩国栋 李翠 刘惠 刘秋艳 《植物研究》 CAS CSCD 北大核心 2007年第6期741-745,共5页
用ISSR分子标记对内蒙古地区刺叶柄棘豆(Oxytorpis aciphylla Ledeb.)5个地理种群进行了种群遗传多样性分析。结果表明:刺叶柄棘豆种群具有较高的遗传多样性,11个ISSR引物扩增出215条带,总的多态位点百分率为98.14%,Shannon多样性指数I=... 用ISSR分子标记对内蒙古地区刺叶柄棘豆(Oxytorpis aciphylla Ledeb.)5个地理种群进行了种群遗传多样性分析。结果表明:刺叶柄棘豆种群具有较高的遗传多样性,11个ISSR引物扩增出215条带,总的多态位点百分率为98.14%,Shannon多样性指数I=0.2108,Nei基因多样性指数H=0.341 6,种内总基因多样性(Ht)为0.2108,种群内基因多样性(Hs)为0.160 4,大部分遗传变异(76.11%)的遗传变异存在于种群内,23.89%的遗传变异存在于种群间。遗传分化系数(Gst)为0.238 9,基因流(Nm)为1.592 9。5个居群间已有遗传分化趋势,遗传漂变不会引起遗传分化。UPGMA遗传距离聚类结果表明,5个地理种群中,植被类型为荒漠草原的4个种群之间遗传距离较近,与1个荒漠种群距离较远。 展开更多
关键词 刺叶柄棘豆 IssR 遗传多样性 Nei指数
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