The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the ...The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling(CK),even the seedlings treated with lethal oxytetracycline concentration.展开更多
In order to probe the death reason of terminal buds and carry out trunk extension by using lateralbuds, the temperature characteristics of dormancy development of terminal and lateral buds in Paulowniatomentosa p. for...In order to probe the death reason of terminal buds and carry out trunk extension by using lateralbuds, the temperature characteristics of dormancy development of terminal and lateral buds in Paulowniatomentosa p. fortunei 33 were investigated by raising cutting branches on different date at 25 ℃ and 15 ℃.The results were as follows: ①The death reason of terminal buds and the first pair of lateral buds was itselfecological adapting strategy, not the injury of early frost and freezing; ②The lateral buds 2, 3 and 4 hadobtained the feature of winter dormancy, and could survive from cold winter, and widen its possible temperaturerange of sprouting to lower temperature by experiencing winter chilling; ③The lateral buds 2, 3 and 4 couldsprout at the same time in spring, but it was difficult to sprout for other lateral buds; ④The sprouting ofupper lateral buds could restrict the sprouting of lower lateral buds at 25 ℃, but not at 15 ℃.展开更多
文摘The effects of oxytetracycline on the morphology of the seedling with witches’ broom and global DNA methylation level of Paulownia tomentosa × Paulownia fortunei were investigated.The results indicated that the infected seedlings by phytoplasma recovered to normal morphology of the healthy seedlings after being treated with more than 20 mg L-1 oxytetracycline,and the pathogen could not be detected with PCR in the seedlings.The global DNA methylation level of diseased seedlings was lower than that of healthy one.The oxytetracycline treatment was able to increase the DNA methylation level,and the methylation level of the diseased seedlings increased with the dose of applied oxytetracycline.However the increased methylation of infected seedlings with oxytetracycline treatment could still not reach to level of healthy seedling(CK),even the seedlings treated with lethal oxytetracycline concentration.
基金Foundation of Research Startup for Returned Student From AbroadScience Foundation of Henan Province forDistiguished Young Scholars(NO.002).
文摘In order to probe the death reason of terminal buds and carry out trunk extension by using lateralbuds, the temperature characteristics of dormancy development of terminal and lateral buds in Paulowniatomentosa p. fortunei 33 were investigated by raising cutting branches on different date at 25 ℃ and 15 ℃.The results were as follows: ①The death reason of terminal buds and the first pair of lateral buds was itselfecological adapting strategy, not the injury of early frost and freezing; ②The lateral buds 2, 3 and 4 hadobtained the feature of winter dormancy, and could survive from cold winter, and widen its possible temperaturerange of sprouting to lower temperature by experiencing winter chilling; ③The lateral buds 2, 3 and 4 couldsprout at the same time in spring, but it was difficult to sprout for other lateral buds; ④The sprouting ofupper lateral buds could restrict the sprouting of lower lateral buds at 25 ℃, but not at 15 ℃.