The fall armyworm,Spodoptera frugiperda(S.frugiperda),represents the most resistant insect species and poses serious threat to grain yield.Chlorantraniliprole(CHL),which targets the ryanodine receptors(RyRs)in insects...The fall armyworm,Spodoptera frugiperda(S.frugiperda),represents the most resistant insect species and poses serious threat to grain yield.Chlorantraniliprole(CHL),which targets the ryanodine receptors(RyRs)in insects,has demonstrated the efficacy in controlling S.frugiperda.Nevertheless,this has led to emerging resistance in several countries.To counter this resistance,a viable approach involves the development of novel compounds that bind against RyRs via distinct binding sites or modes.In this study,a series of 22 novel anthranilic diamide derivatives was designed and synthesized,and their insecticidal activities were evaluated.Most of these derivatives showed moderate to good insecticidal activity against S.frugiperda and Mythimna separata.Time-lapse fluorescence measurements of endoplasmic reticulum luminal calcium revealed that most derivatives elicited cellular responses similar as CHL when assessed on HEK293 cells expressing S.frugiperda ryanodine receptors(SfRyRs).The mode of action of compound 13a was studied and verified on the isolated neurons by calcium imaging technique.Finally,molecular docking analysis was employed to predict the binding mechanism of compound 13a against SfRyRs.Overall,these novel diamide derivatives hold promise as a valuable resource for guiding the future design of insecticidal compounds targeting RyRs.展开更多
I. INTRODUCTION Hematoporphyrin derivative (HPD) has been used as a photosensitizer in the laser photoradiation therapy of malignant tumors as well as in the diagnosis of cancer, and its clinical value has been widely...I. INTRODUCTION Hematoporphyrin derivative (HPD) has been used as a photosensitizer in the laser photoradiation therapy of malignant tumors as well as in the diagnosis of cancer, and its clinical value has been widely recognized.Many studies have shown that the展开更多
I. INTRODUCTION Derivative action was introduced into the Chinese Company Law for the very first time during the revision process in 2005. Some scholars have argued that derivative action was actually permitted under ...I. INTRODUCTION Derivative action was introduced into the Chinese Company Law for the very first time during the revision process in 2005. Some scholars have argued that derivative action was actually permitted under the Chinese Company Law of 1993 because article 111 of the Chinese Company Law of 1993 provided that shareholders have the right to raise a suit when directors violate the law.1 However, main stream scholars object to this view and assert that this article was only intended for shareholders1 personal actions in joint stock companies.展开更多
基金Nankai University Cangzhou Bohai New Area Green Chemical Research Institute(NCC)Fund,the National Natural Science Foundation of China(Nos.31972287,32022073)the National Key Research and Development Program of China(No.2022YFE0108400)the Scientific Project of Tianjin Municipal Education Commission,China(No.2022KJ026).
文摘The fall armyworm,Spodoptera frugiperda(S.frugiperda),represents the most resistant insect species and poses serious threat to grain yield.Chlorantraniliprole(CHL),which targets the ryanodine receptors(RyRs)in insects,has demonstrated the efficacy in controlling S.frugiperda.Nevertheless,this has led to emerging resistance in several countries.To counter this resistance,a viable approach involves the development of novel compounds that bind against RyRs via distinct binding sites or modes.In this study,a series of 22 novel anthranilic diamide derivatives was designed and synthesized,and their insecticidal activities were evaluated.Most of these derivatives showed moderate to good insecticidal activity against S.frugiperda and Mythimna separata.Time-lapse fluorescence measurements of endoplasmic reticulum luminal calcium revealed that most derivatives elicited cellular responses similar as CHL when assessed on HEK293 cells expressing S.frugiperda ryanodine receptors(SfRyRs).The mode of action of compound 13a was studied and verified on the isolated neurons by calcium imaging technique.Finally,molecular docking analysis was employed to predict the binding mechanism of compound 13a against SfRyRs.Overall,these novel diamide derivatives hold promise as a valuable resource for guiding the future design of insecticidal compounds targeting RyRs.
文摘I. INTRODUCTION Hematoporphyrin derivative (HPD) has been used as a photosensitizer in the laser photoradiation therapy of malignant tumors as well as in the diagnosis of cancer, and its clinical value has been widely recognized.Many studies have shown that the
基金funded by National Social Science Funding Project(17CFX072)Southwest University of Political Science and Law 2018 Key Projects and the 19th Special Project(2017)
文摘I. INTRODUCTION Derivative action was introduced into the Chinese Company Law for the very first time during the revision process in 2005. Some scholars have argued that derivative action was actually permitted under the Chinese Company Law of 1993 because article 111 of the Chinese Company Law of 1993 provided that shareholders have the right to raise a suit when directors violate the law.1 However, main stream scholars object to this view and assert that this article was only intended for shareholders1 personal actions in joint stock companies.
