The effect of shark cartilage extract (SCE), purified in this laboratory, on angiogenesis in chick chorioallantoic membrane (CAM), on the activity of collagenase IV and on human umbilical vein endothelial cell (ECV 3...The effect of shark cartilage extract (SCE), purified in this laboratory, on angiogenesis in chick chorioallantoic membrane (CAM), on the activity of collagenase IV and on human umbilical vein endothelial cell (ECV 304) proliferation and apoptosis was investigated in vitro. The results showed that SCE caused a decline in CAM blood vessels and significantly prevented collagenase induced collagenolysis. Moreover, SCE produced a dose dependent decline in ECV 304 proliferation and altered its normal cell cycle. These results suggest that the anti angiogenesis and anti tumor effects of shark cartilage may be due to inhibition of endothelial cells as well as collagenolysis.展开更多
Shark cartilage powder and extract made in our laboratory, referred to SCE(shark cartilage extract), were used to investigate the effects of shark cartilage on the tumor cell lines HeLa and BEL 7404, tumor bearing mo...Shark cartilage powder and extract made in our laboratory, referred to SCE(shark cartilage extract), were used to investigate the effects of shark cartilage on the tumor cell lines HeLa and BEL 7404, tumor bearing mouse S 180 , Heps, EAC, and several types of immune cell. The results have shown that SCE significantly inhibits tumor growth, reducing tumors rate by 60% 70%. In addition, the activities of natural killer (NK) cells and interleukin activated killer (LAK) cells to the target cells Yac -1 and P 815 are increased after treatment with SCE, suggesting a possible mechanism for increasing tumor monitoring and inhibition activities.展开更多
基金the National High Technology Researchand Development Program of China (No. 819- 0 5 - 0 1and No. 819- Z- 0 5 )
文摘The effect of shark cartilage extract (SCE), purified in this laboratory, on angiogenesis in chick chorioallantoic membrane (CAM), on the activity of collagenase IV and on human umbilical vein endothelial cell (ECV 304) proliferation and apoptosis was investigated in vitro. The results showed that SCE caused a decline in CAM blood vessels and significantly prevented collagenase induced collagenolysis. Moreover, SCE produced a dose dependent decline in ECV 304 proliferation and altered its normal cell cycle. These results suggest that the anti angiogenesis and anti tumor effects of shark cartilage may be due to inhibition of endothelial cells as well as collagenolysis.
文摘Shark cartilage powder and extract made in our laboratory, referred to SCE(shark cartilage extract), were used to investigate the effects of shark cartilage on the tumor cell lines HeLa and BEL 7404, tumor bearing mouse S 180 , Heps, EAC, and several types of immune cell. The results have shown that SCE significantly inhibits tumor growth, reducing tumors rate by 60% 70%. In addition, the activities of natural killer (NK) cells and interleukin activated killer (LAK) cells to the target cells Yac -1 and P 815 are increased after treatment with SCE, suggesting a possible mechanism for increasing tumor monitoring and inhibition activities.
