Objectives:The purpose of this paper was to construct a reliable methodology to discriminate the geographical origins of Chuanminshen violaceum Sheh et Shan planted in different regions in Sichuan,China.Materials and ...Objectives:The purpose of this paper was to construct a reliable methodology to discriminate the geographical origins of Chuanminshen violaceum Sheh et Shan planted in different regions in Sichuan,China.Materials and methods:Fatty acid profiles of roots and leaves of C.violaceum planted in various regions of Sichuan Province in China,namely Guangyuan(GY),Langzhong(LZ),Jintang(JT),Bazhong(BZ),and Shuangling(SL),were determined using GC-MS followed by multivariate statistical analyses,including orthogonal partial least-squares discriminant analysis and hierarchical clustering analysis.Results:Leaves of C.violaceum showed the highest contents of hexadecatrienoic acid(3.21 g/kg),linoleic acid(6.62 g/kg),andα-linolenic acid(7.24 g/kg),which were all higher than those contained in roots.Chuanminshen violaceum samples collected from LZ,JT,and GY could be clearly distinguished based on fatty acid profiles of leaves and those collected from LZ,GY,and BZ could be clearly distinguished based on fatty acid profiles of roots.Conclusions:Chemometric method is used as a potential approach for analyses of fatty acid profiles of roots and leaves to control the quality of C.violaceum and their powered products.展开更多
文摘采用PCR直接测序法对伞形科（ Apiaceae）前胡族（ Trib. Peucedaneae）种类川明参（ Chuanminshen violaceum Sheh et Shan）、泰山前胡〔Peucedanum wawrae （ H. Wolff） Su〕和华中前胡（ P. medicum Dunn）以及美味芹族（ Trib. Smyrnieae）种类明党参（Changium smyrnioides H. Wolff）、宝兴棱子芹（Pleurospermum davidii Franch.）、丽江棱子芹（ P. foetens Franch.）和鸡冠棱子芹（ P. cristatum de Boiss.）的叶绿体基因组psbA-trnH片段进行了扩增测序,获得的序列已提交至GenBank,登录号为KF557756-KF557762。结合引自GenBank的前胡族阿魏属（ Ferula Linn.）1种、大瓣芹属（ Semenovia Regel et Herder）1种、当归属（ Angelica Linn.）2种和美味芹族的舟瓣芹属（ Sinolimprichtia H. Wolff）1种、羌活属（ Notopterygium de Boiss.）1种、瘤果芹属（ Trachydium Lindl.）1种以及针果芹族（ Trib. Scandicineae）刺果芹〔Turgenia latifolia （ Linn.） Hoffm.〕的psbA-trnH片段序列,对各种类的psbA-trnH片段信息进行分析；并以刺果芹为外类群构建了MP、ML和BI系统发育树。结果表明：川明参和明党参的psbA-trnH片段长度均为258 bp、GC含量均为23％,而其他种类的psbA-trnH片段长度为228~405 bp、GC含量为26％~35％；排序后psbA-trnH 序列总长度为553 bp（包括空位）,其中变异位点237个、信息位点178个。川明参与明党参间的相对遗传距离最小（仅为0.02）,而川明参与其他种类间的相对遗传距离为0.10~1.34,且总体上川明参与美味芹族种类的相对遗传距离较小,表明川明参与明党参及美味芹族种类的亲缘关系较近。在3类系统树上,川明参与明党参均聚在一起,并与美味芹族的属种聚为一大支,而远离由前胡族属种构成的另一大支。结合外部形态和果实解剖结构特征,建议将川明参属（ Chuanminshen Sheh et Shan ）从前胡族中分出并置于美味芹族中,与明党参属（ Changium H. Wolff）为姐妹类群。
基金This work was supported financially by the Education Department of Sichuan Province under Grant 17ZA0304.
文摘Objectives:The purpose of this paper was to construct a reliable methodology to discriminate the geographical origins of Chuanminshen violaceum Sheh et Shan planted in different regions in Sichuan,China.Materials and methods:Fatty acid profiles of roots and leaves of C.violaceum planted in various regions of Sichuan Province in China,namely Guangyuan(GY),Langzhong(LZ),Jintang(JT),Bazhong(BZ),and Shuangling(SL),were determined using GC-MS followed by multivariate statistical analyses,including orthogonal partial least-squares discriminant analysis and hierarchical clustering analysis.Results:Leaves of C.violaceum showed the highest contents of hexadecatrienoic acid(3.21 g/kg),linoleic acid(6.62 g/kg),andα-linolenic acid(7.24 g/kg),which were all higher than those contained in roots.Chuanminshen violaceum samples collected from LZ,JT,and GY could be clearly distinguished based on fatty acid profiles of leaves and those collected from LZ,GY,and BZ could be clearly distinguished based on fatty acid profiles of roots.Conclusions:Chemometric method is used as a potential approach for analyses of fatty acid profiles of roots and leaves to control the quality of C.violaceum and their powered products.
