Regulatory Effect of Shenge Yifei Capsule on TGF-β1/Smad Signaling Pathway in Rats with Chronic Obstructive Pulmonary Disease

[Objectives]This study aimed to study the effects of Shenge Yifei capsule on the TGF-β1/Smad signaling pathway in rats with chronic obstructive pulmonary disease(COPD).[Methods]Ten rats were randomly selected as the control group,and the other 40 rats were selected for modeling by fumigation combined with Klebsiella pneumoniae infection.A total of 38 rats were successfully modeled.They were randomly divided into model group(8 rats),low-dose Shenge Yifei capsule group(10 rats),high-dose Shenge Yifei capsule group(10 rats)and theophylline group(10 rats)in accordance with the principle of half male and half female.The rats in the model and control groups were given with distilled water by gavage,and the rats in the drug administration groups were given with corresponding drugs.The TGF-β1 level in the serum,and the expression levels of TGF-β1,Smad2,Smad3 and Smad7 and TGF-β1,Smad3 and Smad7 in airway tissues were detected.[Results]After 12 weeks,the serum TGF-β1 levels of the theophylline group and high-dose Shenge Yifei capsule group were lower than that of the low-dose Shenge Yifei capsule group(P<0.05).The expression levels of TGF-β1 and Smad3 in the theophylline group and high-dose Shenge Yifei capsule group were lower than that in the low-dose Shenge Yifei capsule group(P<0.05).The expression levels of TGF-β1 and Smad3 in the high-dose Shenge Yifei capsule group were lower than those in the low-dose Shenge Yifei capsule group and theophylline group(P<0.05).The expression levels of Smad7 and the proteins in the model group were lower than those in the other groups(P<0.05).The expression levels of Smad7 in the theophylline group and high-dose Shenge Yifei capsule group were higher than that in the low-dose Shenge Yifei capsule group(P<0.05).After 18 weeks,no significant difference was found in serum TGF-β1 level among the theophylline group and low and high-dose Shenge Yifei capsule groups(P>0.05).The expression levels of Smad7 and the proteins in the model group were lower than those in the other groups.The expression level of Smad7 in the high-dose Shenge Yifei capsule group was lower than that in the theophylline group(P<0.05).[Conclusions]Shenge Yifei capsule can regulate the TGF-β1/Smads signaling pathway.They can down-regulate the expression of TGF-β1,Smad2 and Smad3 and up-regulate the expression of Smad7,reducing the degree of airway modeling,delaying the development of COPD disease.Conventional high-dose Shenge Yifei capsule is more effective in inhibiting the expression of Smad2.

Antifibrosis effects of Shenge Yangfei Capsules on bleomycin-induced pulmonary fibrosis in rats

Objective： The present study, for the first time, was designed to examine the antifibrosis effects of Shenge Yangfei Capsules（SGYF） on bleomycin-induced pulmonary injury and fibrosis in rats.Methods： Animals were divided into six groups randomly： saline control group, model group（bleomycin5 mg/kg）, and positive control group（Dexamethasone 5 mg/kg）, and SGYF（70, 420, and 850 mg/kg/d, ig）group. Effects of SGYF on body weight and lung index were evaluated. For histological evaluation of lungs injury and fibrosis, lung specimens were stained with hematoxylin and eosin（HE） and Masson trichrome.Body weight and lung index from various groups were measured, and the concentrations of monoamine（MAO） and transforming growth factor-β1（TGF-β1） were also detected in lung homogenates.Results： SGYF attenuated the bleomycin-induced weight loss and the lung index. Histologica levidence showed the ability of SGYF to decrease bleomycin-induced lung fibrosis and consolidation. SGYF reduced MAO and TGF-β1 activity in lung tissues.Conclusions： These findings suggest that SGYF may be a promising candidate to prevent bleomycininduced lung damage or other interstitial lesions.

Protective efficacy of Shenge San(参蛤散) on mitochondria in H9c2 cardiomyocytes

OBJECTIVE: To investigate whether peroxisome proliferator-activated receptor γ-coactivator-1α/nuclear respiratory factor 1(PGC-1α/NRF1) activity can protect mitochondrial function in the setting of cardiac hypertrophy and improve cardiomyocyte energy metabolism. METHODS: Cardiac hypertrophy was modeled in H9c2 cells treated with isoproterenol(ISO) to assess the effects of Shenge San( 参 蛤 散, SGS) on cell viability and mitochondrial membrane potential. We assessed mitochondrial complex m RNA levels and mitochondrial oxidative phosphorylation factor m RNA and protein levels. RESULTS: Compared with the 100 μM ISO group, cell size was significantly decreased in the 0.3 mg/m L SGS and 20 μM ZLN005(PGC-1α activator) groups(P < 0.01). Compared with the SGS(0.3) +ISO group, we observed lower phosphorylated adenosine monophosphateactivated kinase(AMPK) protein levels in the ISO and ZLN005+SGS+ISO groups(P < 0.01). Compared with the compound C group, SGS significantly increased PGC-1α expression in ISO-induced cardiac hypertrophy cells(P < 0.01), and this was inhibited by compound C pretreatment(P < 0.05). Compared with the ISO group, the mitochondrial red-green fluorescence ratio increased in the 0.3 mg/m L SGS group(P < 0.05). m RNA levels of cytochrome c oxidase subunit 1(CO1) in the ISO and compound C groups were lower than those in control group(P < 0.01), and the m RNA levels of CO1 and ATP8 were significantly lower in the ISO and compound C groups versus control(P < 0.01). Compared with the SGS(0.3) +ISO group, ATP synthetase subunit 8(ATP8) m RNA was significantly decreased in the ISO group(P < 0.01) and compound C+SGS+ISO group(P < 0.05). Compared with the SGS(0.3) +ISO group, NRF1 m RNA levels were significantly decreased(P < 0.05) in the ISO and compound C+SGS+ISO groups. CONCLUSIONS: SGS can attenuate ISO-induced cardiomyocyte hypertrophy, restore the decrease in mitochondrial membrane potential, and upregulate PGC-1α/NRF1 levels. Notably, these effects can be blocked by AMPK inhibitor-compound C.

Journal Introduction

AJSP has been founded jointly by Shenyang Pharmaceutical University and Hongkong Shengjing Pharmaceutical Publishing&Media Co.,Limited.The journal is published by Hongkong Shengjing Pharmaceutical Publishing&Media Co.,Limited.

The active mechanism of the Sheng Yang San Huo decoction on diabetic peripheral neuropathy on network pharmacology

Objectives:To discuss the mechanism of Sheng Yang San Huo decoction on diabetic peripheral neuropathy using the network pharmacology method.Methods:The BATMAN-TCM database,TCM-ID database,Chinese Natural Product Chemical Composition Database,and TCMIP database were employed to screen the chemical active ingredients of each herb in Sheng Yang San Huo decoction based on the“Libinsky Drug Rules”.SwissTargetPrediction was used to screen effective action targets for each herb in the prescription.Additionally,Cytoscape 3.7.0 was utilized to construct a“drug-target”network.GeneCards,OMIM,and MaLaCards databases were utilized to gather targets related to diabetic peripheral neuropathy.VENNY 2.1 online platform was employed to match drug and disease targets,draw a Venn diagram,and construct a“drug-active compounds-common target”network using Cytoscape 3.7.0.gene ontology biological process analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis for the targets were conducted using the DAVID 6.8 database.Enrichment analysis results were visualized using the OmicShareTool online platform.Molecular docking was performed using CB-Dock2.Results:Following screening,a total of 217 active compounds and 132 potential targets were identified in Sheng Yang San Huo decoction.The effects are primarily enriched in pathways such as Lipid and Atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,and the IL-17 signaling pathway.The binding energy of the key active ingredients to the core protein targets of DPN was favorable.Conclusion:The study reveals the characteristics of multiple targets and pathways of Sheng Yang San Huo decoction,providing new insights for the clinical application of this prescription.

The 3,300-ton rare earth oxide project of Shenghe Resources to be completed and put into production by the end of 2023

Shenghe Resources recently said in an investor survey that the company has a recycling plant in Jiangxi province,capable of processing NdFeB and phosphor slags.The 3,300-ton rare earth oxide project currently under construction is also a recycling plant,and is expected to be completed and put into operation by the end of 2023.

Translator Stylistics:A Corpus Analysis of Two English Translations of Traditional Chinese Medicine Classic Da Sheng Bian

Da Sheng Bian,a significant work on obstetrics and gynecology that emerged in the early Qing Dynasty,was initially published as“A Treatise on Midwifery”in 1842 by William Lockhart,a British missionary to China.In 1894,John G.Kerr,an American missionary,translated the text as“The Tat Shang Pin”.This paper conducts a comparative study of the two English translations using a self-constructed English-Chinese parallel corpus of Da Sheng Bian.The study explores the translation styles of the two translators by examining the token-types ratio and frequency at the lexical level,mean sentence length at the syntactic level,and the use of conjunctions at the discourse level.The observed differences in translation styles between the two translations are analyzed in relation to the translators’backgrounds and translation strategies.

夏季东海水团变动特征及对鲐鲹渔场的影响

应用模糊聚类方法，采集东海３０个站表层及底层１９９６－１９９８年夏季的水温（Ｔ）、盐度（Ｓ）、溶解氧（ＤＯ）、磷（Ｐ）、三态氮（Ｎ）、硅（Ｓｉ）、ｐＨ值等九个指标的测样数据，对东海水团进行划分，其表层水团配置为大陆沿岸冲淡水（Ｉ），黄东海混合水团（Ⅱ），台湾暖流水（Ⅲ），黑潮表层水（Ⅳ）等水团。底层水团配置为黑潮次表层水团（Ｖ），黄海冷水团（Ⅵ），台湾暖流水（Ⅲ）等水团。并分析了１９９６－１９９８年夏季由大陆径流和外海水团势力强弱影响所致的东海水团变动特征及沿岸冲淡水转向问题，同时讨论了东海水团分布与鲐■渔场关系。

伸梗獐牙菜的甙类成分

从伸梗獐牙菜的乙醇提取物中分得7个甙类成分，经化学和波谱方法，确定它们的结构分别为2′－间羟基本甲酰獐牙菜甙（2′－m－hydroxybenzoylswyroside，Ⅰ），獐牙菜苦甙（swertiamarin，Ⅱ），（＋）－hrdroxyinoresinol－1－O－β－D－glucoside（Ⅲ），8－O－β－D－葡萄糖－1，3，5－三羟基酮（8－O－β－D－glucopyranosyl－1，3，5－trihydroxyxanthone，Ⅳ），8－O－β－D－葡萄糖－1，5－二羟基－3－甲氧基酮（8－O－β－D－glucopyranosyl－1，5dihydroxy－3－methoxyxanthone，Ⅴ），2－β－D－葡萄糖－1，3，6，7－四羟基酮（2－β－D－glucopyranosyl－1，3，6，7－tetrahydroxyxanthone，Ⅵ），4－β－D－葡萄糖－1，3，6，7－四羟基酮（4－β－D－glucopyranosyl－1，3，6，7－tetrahydroxyxanthone，Ⅶ）。该7个甙类成分均系首次从本植物中分得。

生血宁治疗缺铁性贫血的临床研究

蚕砂中提取叶绿素、经皂化、铁代精制成铁叶绿酸钠,并以此为原料制成生血宁片,治疗缺铁性贫血180例,以硫酸亚铁片作对照。结果成人治疗组120例,治愈108例占90％,显效12例占10％;成人对照组30例治愈10例,占33.33％,显效8例占26.67％,有效6例占20％,无效6例占20％。儿童治疗组60例,治愈52例占86.67％,显效8例占13.33％。儿童对照组30例,治愈13例占43.33％,显效5例占16.67％,有效4例占13.33％,无效8例占26.67％,生血宁片疗效明显优于硫酸亚铁片,且无明显毒副反应,易为广大病人接受值得推广应用。

禅宗心灵的表现──日本著名景观设计师■野俊明及其作品

■野俊明被誉为最具日本传统园林风格的现代景观设计师。由于他出生在一个禅僧世家，所以从他的作品中可以充分体验到日本传统文化在景观设计中的表现。

四生汤抗放射反应作用机制的实验研究

在临床研究中,已经发现四生汤具有抗放疗毒副反应,提高机体免疫功能的作用,为了进一步探索该方的作用机理.我们以60Co 照射大鼠为实验对象,较系统地观察了四生汤对模型动物神经内分泌免疫网络内各不同层次,不同环节的影响及对照射大鼠下丘脑β-内啡肽含量的影响。

《梦溪笔谈》所记毕昇泥活字印书法释论

北宋沈括的《梦溪笔谈》卷十八《技艺门》记载:'板印书籍,唐人尚未盛为之,自冯瀛王始印《五经》,已后典籍皆为板本。(1)庆历中,有布衣毕昇又为活板。(2)其法:用胶泥刻字,薄如钱唇,每字为一印,火烧令坚。(3)先设一铁板,其上以松脂蜡和纸灰之类冒之。(4)欲印,则以一铁范置铁板上,乃密布字印,满铁范为一板,持就火炀之。(5)

略论毕昇活字印刷术的发明与发展(二)

④1991年,在宁夏贺兰县拜寺沟西夏方塔第十层塔心室内发现一批西夏时期文物遗存。其中西夏文佛经《吉祥遍至口和本续》,经考古学家牛达生先生悉心研究、考证,认定为是12世纪下半期(1150～1180)木活字印本,并通过了由文化部主持的部级鉴定。这是迄今所知存世最早的木活字印本遗存。 ⑤二十世纪五十年代以来,学术界还先后发现了清代江苏李瑶(1790～1855)、安徽翟金生(1775～1860?)

《长生殿》“三易稿”创作时间考

本文根据洪昇的作品及当时人的记载,汲取现当代学者的成果,除了认定《长生殿》最终定稿于康熙二十七年（1688）外,还指出《沉香亭》成稿于康熙十二年（1673）或康熙十六年（1677）和《舞霓裳》成稿于康熙十八年（1679）的观点难以成立,进而认为《沉香亭》的创作时问当为康熙十四年（1675）,《舞霓裳》的创作时间至迟不会超过康熙十五年（1676）。

甘肃马泉金矿伴生元素特征及剥蚀程度研究

对马泉金矿伴生元素的一般特征、组合特征和垂直分带做了细致的研究 ,并以此为基础 ,进一步对矿床的剥蚀深度做了较详细论证。马泉金矿矿石伴生元素的垂直分带 (由上至下 )为Bi—Cu—Hg—Ag—Pb—Sb—Au—As—Zn—Be—Mo ;与Au相关的元素依次为As、Sb、Cu、Zn、Ag。矿床已剥蚀到中上部 。

Prof.Sheng Canruo's Experience in Acupuncture Treatment of Throat Diseases with Yan Si Xue

Yan Si Xue(咽四穴)refer to four points in the throat summarized by Prof.Sheng Canruo in his long-year clinical experience based on the combination of TCM theory and the knowledge of modernmedical anatomy.By taking “Yan Si Xue” as main points,and other differential adjunct points,Prof.Sheng has obtained satisfactory therapeutic results in treating various throat diseases such ashoarseness,paralysis of vocal cord,dysphonia after radiotherapy on throat tumor,vocal nodules,disorder of the glossopharyngeal nerve,hysteric aphasia,and acute or chronic laryngopharyngitis.

The Song Tomb Fresco (宋墓壁画) in Panle Village of Hancheng City (韩城盘乐村):The Medicine‑preparation Picture (备药图)

A song tomb was discovered in Panle village(盘乐村)of Hancheng city(韩城),Shaanxi Province(陕西省)in 2009.Although it was not of high standard,the tomb contained extremely exquisite frescoes with striking contents.The relation between the identity of the tomb owner and the frescoes had been discussed by scholars,while this paper focused on the social status of doctors in the Song dynasty(宋朝)and the medical scenes reflected in the frescoes,to form different perspectives toward the profession of the tomb owner and the properties of the frescoes.