Background:Hyperglycemia is a characteristic feature of diabetes that often results in neuropsychological complications such as depression.Diabetic individuals are more vulnerable to experience depression compared to ...Background:Hyperglycemia is a characteristic feature of diabetes that often results in neuropsychological complications such as depression.Diabetic individuals are more vulnerable to experience depression compared to the normal population.Thus,novel treatment approaches are required to reduce depressive symptoms among diabetic individuals.Traditional Chinese medicines(TCMs)such as Shengmai San(SMS)and Radix puerariae(R)are usually widely used to treat ailments such as neurological com-plications since ancient time.Methods:In this study,SMS was combined with R to prepare an R-SMS formulation and screened for their antidepressant activity in diabetic rats.The antidepressant po-tential of the prepared combination was evaluated behaviorally using open field test,novelty-induced hypophagia,and forced swim test in diabetic rats with biochemical and protein expression(PI3K,BDNF[brain-derived neurotrophic factor],and SYN[pr-esynaptic vesicle protein])analysis.Results:Diabetic rats(streptozotocin,45 mg/kg)showed elevated fasting blood glu-cose(FBG)>12 mM with depressive symptoms throughout the study.Treatment with R-SMS(0.5,1.5,and 4.5 g/kg)significantly reverted depressive symptoms in diabetic rats as evinced by significantly(p<0.05)reduced immobility time with an increased tendency to eat food in a novel environment.Treatment with R-SMS also significantly increased the protein expression of PI3K,BDNF,and SYN protein,which play a crucial role in depression.Conclusion:This study showed that R-SMS formulation antagonized depressive symptoms in diabetic rats;thus,this formulation might be studied further to develop as an antidepressant.展开更多
Objective:The purpose of this paper is to study the effects of supplemented Shengmai San on the expression of heat shock protein 72(HSP72)and blood biochemistry in peripheral blood of flying-saucer athletes before and...Objective:The purpose of this paper is to study the effects of supplemented Shengmai San on the expression of heat shock protein 72(HSP72)and blood biochemistry in peripheral blood of flying-saucer athletes before and after high temperature training,so as to provide experimental basis for enhancing thermal endurance and preventing heat disease of flying-saucer athletes with anti-heat disease drugs.Methods:24 flying-saucer athletes from Zhejiang province were selected and divided into two groups,including control group and traditional Chinese medicine group with 12 people in each group.Among them,those who did not drink supplemented Shengmai San were in the control group,while those who drank it were in the traditional Chinese medicine group.During summer training,high temperature training task was carried out for 2 weeks.Flying-saucer athletes in traditional Chinese medicine group were given supplemented Shengmai San before training every morning and afternoon.Peripheral venous blood of each group was extracted before experiment and 2 weeks later and lymphocyte HSP72 content,blood routine and biochemical indexes were determined respectively.Results:Before the experiment,there were no abnormal phenomenon blood routine,biochemical test value and HSP72 expression in the control group and the traditional Chinese medicine group.After comparison,there was no significant difference(P>0.05).After the high temperature training,the expression of HSP72 in the Chinese medicine group was significantly higher than that before the training and control group(P<0.05).There were significant differences in white blood cell count before and after the experiment(P<0.05).Conclusion:Taking supplemented Shengmai San can significantly increase expression of HSP72 in up-regulation of flying saucer athletes after thermal stimulation,thus enhancing thermal tolerance and preventing thermal diseases.展开更多
Objective:To study the effect of Shengmai San(生脉散Pulse-activating Powder) in protecting myocardium in the rat of the type 2 diabetic cardiomyopathy(DCM) model.Methods:The DCM rat model was established by combinatio...Objective:To study the effect of Shengmai San(生脉散Pulse-activating Powder) in protecting myocardium in the rat of the type 2 diabetic cardiomyopathy(DCM) model.Methods:The DCM rat model was established by combination of insulin resistance induced by a high-fat diet with intraperitoneal injection of high dose streptozotocin(50 mg/kg).And these rat models were randomly divided into three groups:a normal group(n=12,one of them died),a model group(n=15) and a Shengmai San group(treatment group,n=15).The damage of the myocardium was assessed by electrocardiogram at the twelfth week after modeling,and the blood glucose,cholesterol and triglyceride levels were determined;the content of the left cardiac ventricle myocardial collagen was quantified by Masson staining test;the level of myocardial cell apoptosis was detected with TUNEL apoptosis detection kit;the damage extent of the myocardial sub-cellular structures was observed by electron microscopy;the expression levels of cardiac TSP-1(Thrombospondin-1),TGF-β1(Transforming Growth Ffactor-β) and TRB-3(Tribbles homolog 3) proteins were detected by immunohistochemical method;the expression levels of cardiac TSP-1,A-TGF-β1 and L-TGF-β1 proteins were detected by Western blotting;and the expression levels of TSP-1 and TRB-3 mRNAs were detected by real-time quantitative PCR.Results:Compared with the control group,the blood glucose,cholesterol,triglycerides levels in both the model groups and the Shengmai San group were significantly decreased;the myocardial tissue was less damaged and the collagen content was reduced in the Shengmai San group;the myocardial sub-cellular structure was injured to a lesser extent;the expression levels of myocardial TSP-1,TGF-β1,TRB-3,and TSP-1,A-TGF-β1,L-TGF-β1 and chymase were decreased,and the expression levels of TSP-1 mRNA and TRB-3 mRNA were decreased in both the model groups and the Shengmai San group(the latter was better),.Conclusion:Shengmai San can inhibit myocardial fibrosis in the rat of diabetic cardiomyopathy,and significantly delay the formation of diabetic cardiomyopathy in hyperglycemia rats through multiple pathways.展开更多
Objective:To systematically evaluate the efficacy of Shengmai San in patients with cardiotoxicity of anthracyclines.Methods:Randomized controlled trials(RCTs)were identified by searching China National Knowledge Infra...Objective:To systematically evaluate the efficacy of Shengmai San in patients with cardiotoxicity of anthracyclines.Methods:Randomized controlled trials(RCTs)were identified by searching China National Knowledge Infrastructure(CNKI),Wanfang Database,Chinese Biomedical Literature Database(CBM),Pub Med,Cochrane Library,and Embase Databases from the inceptions until December 2020.The Cochrane Handbook was used to evaluate the risk of bias in the included studies.Data analysis was conducted using Rev Man 5.3 software.Results:Totally 19 RCTs with 2,331 participants were included in this review.Results showed that in improving arrhythmia(13 RCTs,n=1,877,RR=0.37,95%CI 0.25 to 0.52,P<0.00001),the treatment group was superior to the control group.In terms of reducing left ventricular end-diastolic diameter(LVEDD,2 RCTs,n=128,MD=-0.79,95%CI-0.93 to-0.65,P<0.00001)and left ventricular end systolic diameter(LVESD,2 RCTs,n=128,MD=-0.58,95%CI-0.82 to-0.35,P<0.00001),the treatment group was also better than the control group.In reducing myocardial enzymes such as creatine kinase(CK)[(3 RCTs,n=256,SMD=-0.80,95%CI-1.16 to-0.44,P<0.0001),(2 RCTs,n=126,SMD=-0.62,95%CI-0.98 to-0.26,P=0.0007)],the treatment group was superior to the control group.Conclusion:Shengmai San has a positive effect on the treatment of cardiotoxicity from anthracyclines.However,in the future,it is still necessary to conduct high-quality RCTs to verify its efficacy.展开更多
By means of stimulating bilateral phrenic nerves in rabbits, the effect of Shengmai San(SMS) injection on function of normal and fatigue diaphragm was observed with the measuring of diaphragmevoked potential ( DEP) , ...By means of stimulating bilateral phrenic nerves in rabbits, the effect of Shengmai San(SMS) injection on function of normal and fatigue diaphragm was observed with the measuring of diaphragmevoked potential ( DEP) , and the analysis of the power spectrum of diaphragmatic electromyogram ( EMGdi)at spontaneous breathing. The results were as tollows : ( 1 ) After the adminstration ot 2 ml/kg of SMS, ampli-tude of DEP, central frequency, and the high/low trequency ratio were markedly increased. (2) InjectingSMS 2 ml/kg in 30 minutes betore injury could effectively get rid of diaphragmatic fatigue following electricalstimulation of the phrenic nerves. ( 3 ) Injecting SMS in 2 ml/kg after fatigue could enhance the recovery of thediaphragmatic fatigue .展开更多
基金We thank the National High-End Foreign Experts Recruitment Plan(G2022051012L)National Key Research and Development Program of China(2016YFE0131800)+1 种基金Science and Technology Department of Sichuan Province(2019YFH0023)the High-End Talents Recruitment Program(Liu Xinmin group)of Luzhou Municipal People's Government.
文摘Background:Hyperglycemia is a characteristic feature of diabetes that often results in neuropsychological complications such as depression.Diabetic individuals are more vulnerable to experience depression compared to the normal population.Thus,novel treatment approaches are required to reduce depressive symptoms among diabetic individuals.Traditional Chinese medicines(TCMs)such as Shengmai San(SMS)and Radix puerariae(R)are usually widely used to treat ailments such as neurological com-plications since ancient time.Methods:In this study,SMS was combined with R to prepare an R-SMS formulation and screened for their antidepressant activity in diabetic rats.The antidepressant po-tential of the prepared combination was evaluated behaviorally using open field test,novelty-induced hypophagia,and forced swim test in diabetic rats with biochemical and protein expression(PI3K,BDNF[brain-derived neurotrophic factor],and SYN[pr-esynaptic vesicle protein])analysis.Results:Diabetic rats(streptozotocin,45 mg/kg)showed elevated fasting blood glu-cose(FBG)>12 mM with depressive symptoms throughout the study.Treatment with R-SMS(0.5,1.5,and 4.5 g/kg)significantly reverted depressive symptoms in diabetic rats as evinced by significantly(p<0.05)reduced immobility time with an increased tendency to eat food in a novel environment.Treatment with R-SMS also significantly increased the protein expression of PI3K,BDNF,and SYN protein,which play a crucial role in depression.Conclusion:This study showed that R-SMS formulation antagonized depressive symptoms in diabetic rats;thus,this formulation might be studied further to develop as an antidepressant.
基金the Key Program of Zhejiang Sports Bureau(2017[397]-09)。
文摘Objective:The purpose of this paper is to study the effects of supplemented Shengmai San on the expression of heat shock protein 72(HSP72)and blood biochemistry in peripheral blood of flying-saucer athletes before and after high temperature training,so as to provide experimental basis for enhancing thermal endurance and preventing heat disease of flying-saucer athletes with anti-heat disease drugs.Methods:24 flying-saucer athletes from Zhejiang province were selected and divided into two groups,including control group and traditional Chinese medicine group with 12 people in each group.Among them,those who did not drink supplemented Shengmai San were in the control group,while those who drank it were in the traditional Chinese medicine group.During summer training,high temperature training task was carried out for 2 weeks.Flying-saucer athletes in traditional Chinese medicine group were given supplemented Shengmai San before training every morning and afternoon.Peripheral venous blood of each group was extracted before experiment and 2 weeks later and lymphocyte HSP72 content,blood routine and biochemical indexes were determined respectively.Results:Before the experiment,there were no abnormal phenomenon blood routine,biochemical test value and HSP72 expression in the control group and the traditional Chinese medicine group.After comparison,there was no significant difference(P>0.05).After the high temperature training,the expression of HSP72 in the Chinese medicine group was significantly higher than that before the training and control group(P<0.05).There were significant differences in white blood cell count before and after the experiment(P<0.05).Conclusion:Taking supplemented Shengmai San can significantly increase expression of HSP72 in up-regulation of flying saucer athletes after thermal stimulation,thus enhancing thermal tolerance and preventing thermal diseases.
基金supported by the first grade grant (No. 200070410129)the first batch special grant (No. 200801166) from China Postdoctoral Science Foundation+1 种基金the major project (No. H020920010330)the subject of Science and Technology Plan of Beijing Science and Technology Commission (No. D08050703020802)
文摘Objective:To study the effect of Shengmai San(生脉散Pulse-activating Powder) in protecting myocardium in the rat of the type 2 diabetic cardiomyopathy(DCM) model.Methods:The DCM rat model was established by combination of insulin resistance induced by a high-fat diet with intraperitoneal injection of high dose streptozotocin(50 mg/kg).And these rat models were randomly divided into three groups:a normal group(n=12,one of them died),a model group(n=15) and a Shengmai San group(treatment group,n=15).The damage of the myocardium was assessed by electrocardiogram at the twelfth week after modeling,and the blood glucose,cholesterol and triglyceride levels were determined;the content of the left cardiac ventricle myocardial collagen was quantified by Masson staining test;the level of myocardial cell apoptosis was detected with TUNEL apoptosis detection kit;the damage extent of the myocardial sub-cellular structures was observed by electron microscopy;the expression levels of cardiac TSP-1(Thrombospondin-1),TGF-β1(Transforming Growth Ffactor-β) and TRB-3(Tribbles homolog 3) proteins were detected by immunohistochemical method;the expression levels of cardiac TSP-1,A-TGF-β1 and L-TGF-β1 proteins were detected by Western blotting;and the expression levels of TSP-1 and TRB-3 mRNAs were detected by real-time quantitative PCR.Results:Compared with the control group,the blood glucose,cholesterol,triglycerides levels in both the model groups and the Shengmai San group were significantly decreased;the myocardial tissue was less damaged and the collagen content was reduced in the Shengmai San group;the myocardial sub-cellular structure was injured to a lesser extent;the expression levels of myocardial TSP-1,TGF-β1,TRB-3,and TSP-1,A-TGF-β1,L-TGF-β1 and chymase were decreased,and the expression levels of TSP-1 mRNA and TRB-3 mRNA were decreased in both the model groups and the Shengmai San group(the latter was better),.Conclusion:Shengmai San can inhibit myocardial fibrosis in the rat of diabetic cardiomyopathy,and significantly delay the formation of diabetic cardiomyopathy in hyperglycemia rats through multiple pathways.
基金Supported by the National Natural Science Foundation of China(No.81973689,No.82074254)the Beijing Natural Science Foundation of China(No.7202176)。
文摘Objective:To systematically evaluate the efficacy of Shengmai San in patients with cardiotoxicity of anthracyclines.Methods:Randomized controlled trials(RCTs)were identified by searching China National Knowledge Infrastructure(CNKI),Wanfang Database,Chinese Biomedical Literature Database(CBM),Pub Med,Cochrane Library,and Embase Databases from the inceptions until December 2020.The Cochrane Handbook was used to evaluate the risk of bias in the included studies.Data analysis was conducted using Rev Man 5.3 software.Results:Totally 19 RCTs with 2,331 participants were included in this review.Results showed that in improving arrhythmia(13 RCTs,n=1,877,RR=0.37,95%CI 0.25 to 0.52,P<0.00001),the treatment group was superior to the control group.In terms of reducing left ventricular end-diastolic diameter(LVEDD,2 RCTs,n=128,MD=-0.79,95%CI-0.93 to-0.65,P<0.00001)and left ventricular end systolic diameter(LVESD,2 RCTs,n=128,MD=-0.58,95%CI-0.82 to-0.35,P<0.00001),the treatment group was also better than the control group.In reducing myocardial enzymes such as creatine kinase(CK)[(3 RCTs,n=256,SMD=-0.80,95%CI-1.16 to-0.44,P<0.0001),(2 RCTs,n=126,SMD=-0.62,95%CI-0.98 to-0.26,P=0.0007)],the treatment group was superior to the control group.Conclusion:Shengmai San has a positive effect on the treatment of cardiotoxicity from anthracyclines.However,in the future,it is still necessary to conduct high-quality RCTs to verify its efficacy.
文摘By means of stimulating bilateral phrenic nerves in rabbits, the effect of Shengmai San(SMS) injection on function of normal and fatigue diaphragm was observed with the measuring of diaphragmevoked potential ( DEP) , and the analysis of the power spectrum of diaphragmatic electromyogram ( EMGdi)at spontaneous breathing. The results were as tollows : ( 1 ) After the adminstration ot 2 ml/kg of SMS, ampli-tude of DEP, central frequency, and the high/low trequency ratio were markedly increased. (2) InjectingSMS 2 ml/kg in 30 minutes betore injury could effectively get rid of diaphragmatic fatigue following electricalstimulation of the phrenic nerves. ( 3 ) Injecting SMS in 2 ml/kg after fatigue could enhance the recovery of thediaphragmatic fatigue .
