TGF-Objective:To investigate the effect of postgraduate Shengsui Jiangu Capsule on tumor necrosis factors TNF-α,transforming growth factor TGF-β1 and empty bone lacuna in rats with alcoholic osteoporosis.Method:120 ...TGF-Objective:To investigate the effect of postgraduate Shengsui Jiangu Capsule on tumor necrosis factors TNF-α,transforming growth factor TGF-β1 and empty bone lacuna in rats with alcoholic osteoporosis.Method:120 SD rats were randomly divided into 4 groups,30 rats in each group:blank group,model group,western medicine control group and traditional Chinese medicine intervention group.Liquor gavage was used to model the disease,except for the blank group.The western medicine control group was gavaged with a suspension of calcium carbonate and afad3,while the Chinese medicine intervention group was gavaged with a suspension of Shengsui Jiangu capsules combined with 0.9%saline solution injection.The values of TNF-αand TGF-β1 in femora were measured after 8,12,and 16 weeks of intervention.After dissecting out the femoral head using the HE(hematoxylin-eosin staining)staining method,the bone lacunae were observed under a light microscope,and the empty bone lacunae rate was calculated for intergroup comparison.Results:Compared with the blank group,the model group had a significantly higher percentage of TNF-αand empty bone lacunae,and a significantly lower percentage of TGF-β1(P<0.01).Compared with the model group,the rates of TNF-αand empty bone lacunae in the western medicine control group and the Chinese medicine intervention group were significantly decreased,and TGF-β1 was significantly increased(P<0.01).The traditional Chinese medicine intervention group was more effective than the western medicine control group(P<0.05).Conclusion:Shengsui Jiangu capsule attenuates the expression of TNF-α,enhances the expression of TGF-β1,decreases the rate of empty bone lacunae,and achieves a protective effect against alcoholic osteoporosis in rats with alcoholic osteoporosis.展开更多
Objective:To study the mechanism of Shengsui Jiangu Capsule promoting vitamin D_(3)regulating bone turnover in rats with alcoholic osteoporosis.Methods:120 SD rats were randomly divided into four groups:model group(al...Objective:To study the mechanism of Shengsui Jiangu Capsule promoting vitamin D_(3)regulating bone turnover in rats with alcoholic osteoporosis.Methods:120 SD rats were randomly divided into four groups:model group(alcoholic osteoporosis model group),blank control group,western medicine control group(calcium carbonate tablet and affa D_(3)control group),Chinese medicine experimental group(Shengsui Jiangu capsule experimental group),30 rats in each group.After 8,12 and 16 weeks,the serum levels of 25 hydroxyvitamin D_(3)(25(OH)D_(3)),1,25dihydroxyvitamin D_(3)(1,25(OH)_(2)D_(3)),cross-linked carboxy-terminal peptide of type 1 collagen(β-CTX)and N-terminal propeptide of type 1 collagen(P1NP)in rats and the density of the femur of rats were detected and compared.The correlation between vitamin D_(3)markers(25(OH)D_(3)and 1,25(OH)_(2)D_(3))and bone turnover indices(P1NP andβ-CTX)and bone mineral density was analyzed.Results:In the experimental group,bone mineral density(BMD)was significantly increased(P<0.01),bone repair and mineral stability and balance were maintained during bone turnover,the level of bone formation marker P1NP was significantly increased(P<0.01),and the level of bone resorption markerβ-CTX was significantly decreased(P<0.01).Serum levels of 25(OH)D_(3)and 1,25(OH)_(2)D_(3)were increased(P<0.01),and significantly higher than those in western medicine control group(P<0.05).Conclusion:Shengsui Jiangu capsule can effectively improve bone density in alcoholic osteoporosis(AOP)rats,and its mechanism may be related to promoting bone calcium absorption and regulating bone conversion.展开更多
目的观察理冲生髓饮有效组分(LCSSY)调控腺苷酸活化蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)/丝氨酸苏氨酸蛋白激酶-失调51样激酶1(ULK1)信号通路对卵巢癌自噬的影响。方法体外培养卵巢癌SKOV3细胞,制备LCSSY含药血清,采用随机数...目的观察理冲生髓饮有效组分(LCSSY)调控腺苷酸活化蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)/丝氨酸苏氨酸蛋白激酶-失调51样激酶1(ULK1)信号通路对卵巢癌自噬的影响。方法体外培养卵巢癌SKOV3细胞,制备LCSSY含药血清,采用随机数字表法将SD大鼠分为空白对照组和LCSSY低、中、高剂量组。细胞计数盒8法检测卵巢癌SKOV3细胞增殖抑制率;蛋白质印迹法检测计算自噬相关蛋白重组人自噬效应蛋白(Beclin-1)、人微管相关蛋白轻链3Ⅱ与小鼠微管相关蛋白轻链3Ⅰ比值(LC3Ⅱ/Ⅰ)、自噬接头蛋白(P62)以及AMPK/mTOR/ULK1信号通路相关蛋白的表达,并采用AMPK激活剂验证其作用机制。结果给药24、48 h,LCSSY低、中、高剂量组对SKOV3细胞增殖的抑制率均大于空白对照组,且随着药物浓度的增加抑制程度逐渐加强(均P<0.05);另外,给药48 h LCSSY低剂量组、LCSSY中剂量组抑制率均大于本组给药24 h时(均P<0.05)。与空白对照组比较,LCSSY低、中、高剂量组均能够下调Beclin-1和LC3Ⅱ/Ⅰ的表达,上调P62的表达,且呈剂量依赖性[(0.98±0.02)、(0.59±0.02)、(0.55±0.02)比(1.46±0.12),(4.45±0.21)、(4.00±0.25)、(2.95±0.21)比(5.98±0.23),(0.67±0.02)、(0.94±0.05)、(1.53±0.13)比(0.30±0.02)](均P<0.05)。与空白对照组比较,LCSSY低、中、高剂量组均可以降低AMPK和ULK1表达,升高mTOR复合物1表达,且呈剂量依赖性(均P<0.05)。AMPK激活剂逆转AMPK/mTOR/ULK1信号通路后,与单独LCSSY高剂量组相比,LCSSY高剂量联合AMPK激活剂组使AMPK、ULK1蛋白表达量升高,而mTOR复合物1蛋白表达量降低(均P<0.05)。结论LCSSY可能通过调控AMPK/mTOR/ULK1信号通路抑制卵巢癌细胞自噬。展开更多
目的:探讨补血生髓方对肿瘤相关性贫血(cancer related anemia, CRA)小鼠的改善作用及可能的作用机制。方法:C57BL/6雌性小鼠腹腔注射苯肼(50mg/kg),并且皮下接种肺癌LLC细胞建立CRA模型,随机分成模型组、EPO组(1365IU/kg)、补血生髓方...目的:探讨补血生髓方对肿瘤相关性贫血(cancer related anemia, CRA)小鼠的改善作用及可能的作用机制。方法:C57BL/6雌性小鼠腹腔注射苯肼(50mg/kg),并且皮下接种肺癌LLC细胞建立CRA模型,随机分成模型组、EPO组(1365IU/kg)、补血生髓方组(60.67g/kg),每组10只,另取10只正常小鼠作为空白组,给药2周。ELISA检测促红细胞生成素、血清干扰素-γ(interferon-γ,IFN-γ)水平,流式细胞术检测外周血Tregs细胞比例,Real time-PCR及免疫荧光检测缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、程序性死亡-配体1(programmed cell death ligand 1,PD-L1)mRNA及蛋白的表达。结果:与模型组相比,补血生髓方组小鼠的肿瘤体积、肿瘤质量均显著降低(P<0.01)。与空白组相比,模型组血红蛋白、促红细胞生成素、IFN-γ水平显著下调(P<0.01),外周血Tregs细胞比例显著升高(P<0.05)。与模型组相比,补血生髓方组血红蛋白、促红细胞生成素、IFN-γ水平显著上调(P<0.01),外周血Tregs细胞比例显著下降(P<0.01),HIF-1α、PD-L1mRNA和蛋白表达显著降低(P<0.01)。结论:补血生髓方能够改善CRA小鼠的贫血状态,通过下调HIF-1α/PD-L1通路调节CRA小鼠的免疫功能,从而抑制肿瘤的生长。展开更多
基金Joint Orientation Program of the Heilongjiang Nature Foundation(No.LH2020H086)Chinese Medicine Council of Heilongjiang(No.ZHY10-W22)。
文摘TGF-Objective:To investigate the effect of postgraduate Shengsui Jiangu Capsule on tumor necrosis factors TNF-α,transforming growth factor TGF-β1 and empty bone lacuna in rats with alcoholic osteoporosis.Method:120 SD rats were randomly divided into 4 groups,30 rats in each group:blank group,model group,western medicine control group and traditional Chinese medicine intervention group.Liquor gavage was used to model the disease,except for the blank group.The western medicine control group was gavaged with a suspension of calcium carbonate and afad3,while the Chinese medicine intervention group was gavaged with a suspension of Shengsui Jiangu capsules combined with 0.9%saline solution injection.The values of TNF-αand TGF-β1 in femora were measured after 8,12,and 16 weeks of intervention.After dissecting out the femoral head using the HE(hematoxylin-eosin staining)staining method,the bone lacunae were observed under a light microscope,and the empty bone lacunae rate was calculated for intergroup comparison.Results:Compared with the blank group,the model group had a significantly higher percentage of TNF-αand empty bone lacunae,and a significantly lower percentage of TGF-β1(P<0.01).Compared with the model group,the rates of TNF-αand empty bone lacunae in the western medicine control group and the Chinese medicine intervention group were significantly decreased,and TGF-β1 was significantly increased(P<0.01).The traditional Chinese medicine intervention group was more effective than the western medicine control group(P<0.05).Conclusion:Shengsui Jiangu capsule attenuates the expression of TNF-α,enhances the expression of TGF-β1,decreases the rate of empty bone lacunae,and achieves a protective effect against alcoholic osteoporosis in rats with alcoholic osteoporosis.
基金Joint Orientation Program of the Heilongjiang Nature Foundation(NO.LH2020H086)Chinese Medicine Council of Heilongjiang(NO.ZHY10-W22)。
文摘Objective:To study the mechanism of Shengsui Jiangu Capsule promoting vitamin D_(3)regulating bone turnover in rats with alcoholic osteoporosis.Methods:120 SD rats were randomly divided into four groups:model group(alcoholic osteoporosis model group),blank control group,western medicine control group(calcium carbonate tablet and affa D_(3)control group),Chinese medicine experimental group(Shengsui Jiangu capsule experimental group),30 rats in each group.After 8,12 and 16 weeks,the serum levels of 25 hydroxyvitamin D_(3)(25(OH)D_(3)),1,25dihydroxyvitamin D_(3)(1,25(OH)_(2)D_(3)),cross-linked carboxy-terminal peptide of type 1 collagen(β-CTX)and N-terminal propeptide of type 1 collagen(P1NP)in rats and the density of the femur of rats were detected and compared.The correlation between vitamin D_(3)markers(25(OH)D_(3)and 1,25(OH)_(2)D_(3))and bone turnover indices(P1NP andβ-CTX)and bone mineral density was analyzed.Results:In the experimental group,bone mineral density(BMD)was significantly increased(P<0.01),bone repair and mineral stability and balance were maintained during bone turnover,the level of bone formation marker P1NP was significantly increased(P<0.01),and the level of bone resorption markerβ-CTX was significantly decreased(P<0.01).Serum levels of 25(OH)D_(3)and 1,25(OH)_(2)D_(3)were increased(P<0.01),and significantly higher than those in western medicine control group(P<0.05).Conclusion:Shengsui Jiangu capsule can effectively improve bone density in alcoholic osteoporosis(AOP)rats,and its mechanism may be related to promoting bone calcium absorption and regulating bone conversion.
文摘目的观察理冲生髓饮有效组分(LCSSY)调控腺苷酸活化蛋白激酶(AMPK)/哺乳动物雷帕霉素靶蛋白(mTOR)/丝氨酸苏氨酸蛋白激酶-失调51样激酶1(ULK1)信号通路对卵巢癌自噬的影响。方法体外培养卵巢癌SKOV3细胞,制备LCSSY含药血清,采用随机数字表法将SD大鼠分为空白对照组和LCSSY低、中、高剂量组。细胞计数盒8法检测卵巢癌SKOV3细胞增殖抑制率;蛋白质印迹法检测计算自噬相关蛋白重组人自噬效应蛋白(Beclin-1)、人微管相关蛋白轻链3Ⅱ与小鼠微管相关蛋白轻链3Ⅰ比值(LC3Ⅱ/Ⅰ)、自噬接头蛋白(P62)以及AMPK/mTOR/ULK1信号通路相关蛋白的表达,并采用AMPK激活剂验证其作用机制。结果给药24、48 h,LCSSY低、中、高剂量组对SKOV3细胞增殖的抑制率均大于空白对照组,且随着药物浓度的增加抑制程度逐渐加强(均P<0.05);另外,给药48 h LCSSY低剂量组、LCSSY中剂量组抑制率均大于本组给药24 h时(均P<0.05)。与空白对照组比较,LCSSY低、中、高剂量组均能够下调Beclin-1和LC3Ⅱ/Ⅰ的表达,上调P62的表达,且呈剂量依赖性[(0.98±0.02)、(0.59±0.02)、(0.55±0.02)比(1.46±0.12),(4.45±0.21)、(4.00±0.25)、(2.95±0.21)比(5.98±0.23),(0.67±0.02)、(0.94±0.05)、(1.53±0.13)比(0.30±0.02)](均P<0.05)。与空白对照组比较,LCSSY低、中、高剂量组均可以降低AMPK和ULK1表达,升高mTOR复合物1表达,且呈剂量依赖性(均P<0.05)。AMPK激活剂逆转AMPK/mTOR/ULK1信号通路后,与单独LCSSY高剂量组相比,LCSSY高剂量联合AMPK激活剂组使AMPK、ULK1蛋白表达量升高,而mTOR复合物1蛋白表达量降低(均P<0.05)。结论LCSSY可能通过调控AMPK/mTOR/ULK1信号通路抑制卵巢癌细胞自噬。
