Background:Study the effect of Jiangu Shenjin decoction on the Wnt/β-catenin signalling pathway in knee osteoarthritis.Methods:Forty-eight female Sprague-Dawley rats were randomly divided into eight groups.After succ...Background:Study the effect of Jiangu Shenjin decoction on the Wnt/β-catenin signalling pathway in knee osteoarthritis.Methods:Forty-eight female Sprague-Dawley rats were randomly divided into eight groups.After successful modelling,the blank group and the model group were given a normal diet and drinking water,and the other rats were given meloxicam,an anti-bone hyperplasia agent and Jiangu Shenjin decoction by gavage for 4 weeks.The degree of movement and knee swelling were observed.Serum levels of interleukin-1β,interleukin-6,and tumour necrosis factor-αwere measured.The levels of inflammatory factors,pathological changes in articular cartilage and the mRNA expression levels ofβ-catenin and Wnt4 were examined.Results:Compared with the model group,the treatment group had lower levels than the model group.The medium-dose group had lower levels than the high-dose group.Compared with the meloxicam group and the anti-bone hyperplasia group,the medium-dose group had lower levels than the high-dose group.The medium-dose group had slightly lower levels than the other two groups,and the difference among the three groups was not significant.The mRNA expression level of Wnt4 andβ-catenin indicated thatβ-catenin was inhibited in the signalling pathway.The level ofβ-catenin was inhibited and then increased each treatment,and bone metabolism regulation by Jiangu Shenjin decoction was effective.Conclusion:Jiangu Shenjin decoction may act through Wnt to treat knee osteoarthritis.Theβ-catenin signalling pathway inhibits inflammatory factors and regulates proteins in the signalling pathway to affect bone metabolism.展开更多
基金the General Project of the Natural Science Foundation of Hainan Province(No.819MS064).
文摘Background:Study the effect of Jiangu Shenjin decoction on the Wnt/β-catenin signalling pathway in knee osteoarthritis.Methods:Forty-eight female Sprague-Dawley rats were randomly divided into eight groups.After successful modelling,the blank group and the model group were given a normal diet and drinking water,and the other rats were given meloxicam,an anti-bone hyperplasia agent and Jiangu Shenjin decoction by gavage for 4 weeks.The degree of movement and knee swelling were observed.Serum levels of interleukin-1β,interleukin-6,and tumour necrosis factor-αwere measured.The levels of inflammatory factors,pathological changes in articular cartilage and the mRNA expression levels ofβ-catenin and Wnt4 were examined.Results:Compared with the model group,the treatment group had lower levels than the model group.The medium-dose group had lower levels than the high-dose group.Compared with the meloxicam group and the anti-bone hyperplasia group,the medium-dose group had lower levels than the high-dose group.The medium-dose group had slightly lower levels than the other two groups,and the difference among the three groups was not significant.The mRNA expression level of Wnt4 andβ-catenin indicated thatβ-catenin was inhibited in the signalling pathway.The level ofβ-catenin was inhibited and then increased each treatment,and bone metabolism regulation by Jiangu Shenjin decoction was effective.Conclusion:Jiangu Shenjin decoction may act through Wnt to treat knee osteoarthritis.Theβ-catenin signalling pathway inhibits inflammatory factors and regulates proteins in the signalling pathway to affect bone metabolism.
