As a well-known Chinese medicine prescription, Wenxin Keli (WXKL) has been widely used in the treatment of many arrhythmias including atrial fibrillation and has achieved significant clinical effects, but the specific...As a well-known Chinese medicine prescription, Wenxin Keli (WXKL) has been widely used in the treatment of many arrhythmias including atrial fibrillation and has achieved significant clinical effects, but the specific mechanism of its treatment of atrial fibrillation remains unclear. This study used a network pharmacology approach to reveal the potential molecular mechanisms of WXKL in the treatment of atrial fibrillation. It involves oral bioavailability screening of ingredients, drug-likeness evaluation, compound target fishing, H-C-T network and C-T-P network construction and analysis. Through the network pharmacology-based research strategy, the complex system of WXKL was analyzed. It was found that 14 representative compounds of WXKL, 30 protein targets and 12 related signaling pathways help us systematically understand the underlying mechanism of WXKL’s anti-atrial fibrillation effects.展开更多
目的:基于网络药理学方法分析肾炎消白颗粒治疗糖尿病肾病的潜在机制,为临床运用提供理论参考。方法:挖掘中医药系统药理学平台(TCMSP)获得肾炎消白颗粒有效成分及作用靶点,并研究相关文献进行补充,通过挖掘GeneCards、TTD、OMIM、DisGe...目的:基于网络药理学方法分析肾炎消白颗粒治疗糖尿病肾病的潜在机制,为临床运用提供理论参考。方法:挖掘中医药系统药理学平台(TCMSP)获得肾炎消白颗粒有效成分及作用靶点,并研究相关文献进行补充,通过挖掘GeneCards、TTD、OMIM、DisGeNET数据库获取其疾病潜在靶点,于STRING 11.0平台构建蛋白质互作网络,预测关键靶点并进行基因本体-生物过程分析(Gene Ontology-biological process,GP-BP)及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路分析,构建“肾炎消白颗粒-糖尿病肾病-通路”网络图。结果:肾炎消白颗粒主要有效成分有紫杉碱、花生四烯酸、β-胡萝卜素、β-谷甾醇、联苯酸酯、芒柄花素等,其关键靶点有TNF、RELA、JUN、AKT1、MAPK3等,生物过程可调节多种介质,调控DNA结合转录因子的活性,并针对细胞进行氧化应激反应,其涉及通路主要包括白细胞介素-17(IL-17)信号通路、Toll样受体信号通路、TNF信号通路、糖尿病并发症中的AGE-RAGE信号通路、细胞凋亡、MAPK信号通路、Th17细胞分化通路等。结论:肾炎消白颗粒具有多成分、多靶点、多通路作用的优势,治疗糖尿病肾病可能与炎症反应、自噬-凋亡通路等相关。展开更多
目的:运用网络药理学与分子对接探寻肾炎防衰液治疗肾间质纤维化(RIF)的活性成分和作用机制。方法:通过TCMSP平台和BATMAN-TCM数据库筛选肾炎防衰液的活性成分及作用靶点;利用GeneCards数据库、DisGeNET平台获取RIF相关靶点;将两者的共...目的:运用网络药理学与分子对接探寻肾炎防衰液治疗肾间质纤维化(RIF)的活性成分和作用机制。方法:通过TCMSP平台和BATMAN-TCM数据库筛选肾炎防衰液的活性成分及作用靶点;利用GeneCards数据库、DisGeNET平台获取RIF相关靶点;将两者的共有靶点与其对应的中药成分通过Cytoscape3.7.2软件构建“成分-靶点”网络,利用Centiscape插件筛选出重要的活性成分;通过Clue GO 2.5.4插件对共有靶点进行京都基因与基因组百科全书(KEGG)富集分析,获取肾炎防衰液治疗RIF的潜在作用通路,采用Autodock和PyMol将核心成分和靶点蛋白进行分子对接及可视化处理。结果:肾炎防衰液经筛选后获得200个活性成分,76个参与治疗RIF的潜在靶点,可能通过参与糖尿病并发症的晚期糖基化终末产物(AGE)-晚期糖基化终末产物受体(RAGE)信号通路、流体剪切应力与动脉粥样硬化、肿瘤坏死因子(TNF)信号通路、松弛素信号通路、缺氧诱导因子-1(HIF-1)信号通路、白介素-17(IL-17)信号通路、血管内皮生长因子(VEGF)信号通路、脂肪细胞脂肪分解的调节、磷脂酰肌醇3-激酶(PI3K)-蛋白激酶B(Akt)信号通路、Toll样受体(TLRs)信号通路等途径参与RIF的治疗,分子对接结果提示肾炎防衰液的核心成分与INS、IL-6、ALB、VEGFA、TNF具有较好的亲和力。结论:肾炎防衰液的多种活性成分具改善RIF的作用,该方可能通过抑制炎症反应、调控自噬、调节糖脂代谢等途径延缓RIF的发展。展开更多
文摘As a well-known Chinese medicine prescription, Wenxin Keli (WXKL) has been widely used in the treatment of many arrhythmias including atrial fibrillation and has achieved significant clinical effects, but the specific mechanism of its treatment of atrial fibrillation remains unclear. This study used a network pharmacology approach to reveal the potential molecular mechanisms of WXKL in the treatment of atrial fibrillation. It involves oral bioavailability screening of ingredients, drug-likeness evaluation, compound target fishing, H-C-T network and C-T-P network construction and analysis. Through the network pharmacology-based research strategy, the complex system of WXKL was analyzed. It was found that 14 representative compounds of WXKL, 30 protein targets and 12 related signaling pathways help us systematically understand the underlying mechanism of WXKL’s anti-atrial fibrillation effects.
文摘目的:基于网络药理学方法分析肾炎消白颗粒治疗糖尿病肾病的潜在机制,为临床运用提供理论参考。方法:挖掘中医药系统药理学平台(TCMSP)获得肾炎消白颗粒有效成分及作用靶点,并研究相关文献进行补充,通过挖掘GeneCards、TTD、OMIM、DisGeNET数据库获取其疾病潜在靶点,于STRING 11.0平台构建蛋白质互作网络,预测关键靶点并进行基因本体-生物过程分析(Gene Ontology-biological process,GP-BP)及京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路分析,构建“肾炎消白颗粒-糖尿病肾病-通路”网络图。结果:肾炎消白颗粒主要有效成分有紫杉碱、花生四烯酸、β-胡萝卜素、β-谷甾醇、联苯酸酯、芒柄花素等,其关键靶点有TNF、RELA、JUN、AKT1、MAPK3等,生物过程可调节多种介质,调控DNA结合转录因子的活性,并针对细胞进行氧化应激反应,其涉及通路主要包括白细胞介素-17(IL-17)信号通路、Toll样受体信号通路、TNF信号通路、糖尿病并发症中的AGE-RAGE信号通路、细胞凋亡、MAPK信号通路、Th17细胞分化通路等。结论:肾炎消白颗粒具有多成分、多靶点、多通路作用的优势,治疗糖尿病肾病可能与炎症反应、自噬-凋亡通路等相关。
文摘目的:运用网络药理学与分子对接探寻肾炎防衰液治疗肾间质纤维化(RIF)的活性成分和作用机制。方法:通过TCMSP平台和BATMAN-TCM数据库筛选肾炎防衰液的活性成分及作用靶点;利用GeneCards数据库、DisGeNET平台获取RIF相关靶点;将两者的共有靶点与其对应的中药成分通过Cytoscape3.7.2软件构建“成分-靶点”网络,利用Centiscape插件筛选出重要的活性成分;通过Clue GO 2.5.4插件对共有靶点进行京都基因与基因组百科全书(KEGG)富集分析,获取肾炎防衰液治疗RIF的潜在作用通路,采用Autodock和PyMol将核心成分和靶点蛋白进行分子对接及可视化处理。结果:肾炎防衰液经筛选后获得200个活性成分,76个参与治疗RIF的潜在靶点,可能通过参与糖尿病并发症的晚期糖基化终末产物(AGE)-晚期糖基化终末产物受体(RAGE)信号通路、流体剪切应力与动脉粥样硬化、肿瘤坏死因子(TNF)信号通路、松弛素信号通路、缺氧诱导因子-1(HIF-1)信号通路、白介素-17(IL-17)信号通路、血管内皮生长因子(VEGF)信号通路、脂肪细胞脂肪分解的调节、磷脂酰肌醇3-激酶(PI3K)-蛋白激酶B(Akt)信号通路、Toll样受体(TLRs)信号通路等途径参与RIF的治疗,分子对接结果提示肾炎防衰液的核心成分与INS、IL-6、ALB、VEGFA、TNF具有较好的亲和力。结论:肾炎防衰液的多种活性成分具改善RIF的作用,该方可能通过抑制炎症反应、调控自噬、调节糖脂代谢等途径延缓RIF的发展。