Shewanella sp. ANA-3 with the respiratory arsenate reductase(ArrAB) and MR-1 with ferric reduction ability always coexist in the presence of high arsenic(As)-containing waste residue. However, their synergistic impact...Shewanella sp. ANA-3 with the respiratory arsenate reductase(ArrAB) and MR-1 with ferric reduction ability always coexist in the presence of high arsenic(As)-containing waste residue. However, their synergistic impacts on As transformation and mobility remain unclear. To identify which bacterium, ANA-3 or MR-1, dominates As mobility in the coexisting environment, we explored the As biotransformation in the industrial waste residue in the presence of Shewanella sp. ANA-3 and MR-1. The incubation results show that As(Ⅲ)was the main soluble species, and strain ANA-3 dominated As mobilization. The impact of ANA-3 was weakened by MR-1, probably due to the survival competition between these two bacteria. The results of micro X-ray fluorescence and X-ray photoelectron spectroscopy analyses further reveal the pathway for ANA-3 to enhance As mobility. Strain ANA-3 almost reduced 100% surface-bound Fe(Ⅲ), and consequently led to As(V) release. The dissolved As(V) was then reduced to As(Ⅲ) by ANA-3. The results of this study help to understand the fate of arsenic in the subsurface and highlight the importance of the safe disposal of high As-containing industrial waste.展开更多
从高盐含油废水中分离出一株产生物表面活性剂的菌株YY-1,通过形态学、生理生化特性和16S r RNA序列分析,该菌属于希瓦氏菌属(Shewanella sp.).Shewanella sp.YY-1分泌生物表面活性剂的临界胶束浓度为700mg/L,表面张力为30.76mN/m,且在...从高盐含油废水中分离出一株产生物表面活性剂的菌株YY-1,通过形态学、生理生化特性和16S r RNA序列分析,该菌属于希瓦氏菌属(Shewanella sp.).Shewanella sp.YY-1分泌生物表面活性剂的临界胶束浓度为700mg/L,表面张力为30.76mN/m,且在较宽的盐度(10~100g/L),温度(25~100℃),pH值(2~10)范围内均表现出良好的化学稳定性.通过薄层色谱、傅立叶红外光谱和气相色谱-质谱分析,生物表面活性剂被鉴定为磷脂类.将菌株Shewanella sp.YY-1应用于原油废水的生物修复中,表现出良好的降解效果,对原油中短链烷烃(C8~C10),中链烷烃(C11~C20),长链烷烃(C21~C44)的去除率可达74.70%,96.68%,99.42%.本研究为生物表面活性剂产生菌在原油废水的生物修复提供优质的菌种来源和理论依据.展开更多
分离得到1株对染料活性艳红X-3B具有高效降解作用的菌株,经生理生化鉴定结合16S r DNA分析鉴定为希瓦氏菌属,命名为Shewanella sp.3Y。将该菌固定到活性炭上构建了生物活性炭小试装置,用其处理染料活性艳红X-3B及与其结构类似的7种活性...分离得到1株对染料活性艳红X-3B具有高效降解作用的菌株,经生理生化鉴定结合16S r DNA分析鉴定为希瓦氏菌属,命名为Shewanella sp.3Y。将该菌固定到活性炭上构建了生物活性炭小试装置,用其处理染料活性艳红X-3B及与其结构类似的7种活性染料模拟废水时,水力停留时间2 h,对模拟染料废水COD去除率均在90%以上,对色度去除率在70%以上,大部分达到90%以上,而对UV_(254)的去除率差异较大。展开更多
从患病拟穴青蟹(Scylla paramamosain)肝胰腺中分离到2种优势菌株,对2种优势菌株进行了鉴定和特性分析。利用形态学观察、生理生化特性分析和16S r DNA序列测定法对细菌类别进行判定,并采用药敏试验对细菌的耐药性进行分析。结果表明,这...从患病拟穴青蟹(Scylla paramamosain)肝胰腺中分离到2种优势菌株,对2种优势菌株进行了鉴定和特性分析。利用形态学观察、生理生化特性分析和16S r DNA序列测定法对细菌类别进行判定,并采用药敏试验对细菌的耐药性进行分析。结果表明,这2种细菌分别为弧菌(Vibrio sp.)和希瓦氏菌(Shewanella),利用15种抗生素对2种细菌进行药敏试验分析,发现2种细菌对恩诺沙星、氟苯尼考、强力霉素高度敏感,对其他几种抗生素则有一定的耐受性。研究进一步证实了海水致病性弧菌是拟穴青蟹细菌性疾病高发的主要原因,也表明希瓦氏菌可能是一种潜在病原菌,与弧菌协同作用,对拟穴青蟹规模化养殖造成危害,同时也为拟穴青蟹养殖过程中细菌性病害的确定和防治提供参考依据。展开更多
鉴于弗氏柠檬酸杆菌和迟钝爱德华氏菌是"人-兽-鱼"共患性条件致病菌,因此利用微生物拮抗原理研发微生态制剂进行细菌病害的防治对环境具有友好性.分别以从患病中华鳖分离的弗氏柠檬酸杆菌和迟钝爱德华氏菌作为指示菌,初步筛...鉴于弗氏柠檬酸杆菌和迟钝爱德华氏菌是"人-兽-鱼"共患性条件致病菌,因此利用微生物拮抗原理研发微生态制剂进行细菌病害的防治对环境具有友好性.分别以从患病中华鳖分离的弗氏柠檬酸杆菌和迟钝爱德华氏菌作为指示菌,初步筛选获得对这2种菌具有拮抗作用的菌株6-1-1M和7-1-1M.用点种法和纸片法进一步验证了其拮抗活性,测定了2株菌930 bp的16S r DNA基因片段序列,发现二者序列完全一致,系统进化分析表明其属于希瓦氏菌属(Shewanella sp.).安全性验证实验结果表明其无致病性,具有开发应用前景.展开更多
通过富集分离筛选高效长链烷烃降解菌,采用形态学、生理生化实验、16S r DNA序列比对进行菌种鉴定,通过UV和GC-MS检测菌株对长链烷烃降解特性。结果表明:分离到1株长链烷降解烃菌,经鉴定为希瓦氏菌属,命名为Shewanella sp.LZ02。该菌生...通过富集分离筛选高效长链烷烃降解菌,采用形态学、生理生化实验、16S r DNA序列比对进行菌种鉴定,通过UV和GC-MS检测菌株对长链烷烃降解特性。结果表明:分离到1株长链烷降解烃菌,经鉴定为希瓦氏菌属,命名为Shewanella sp.LZ02。该菌生长温度范围0~45℃,最适生长温度35℃,在接种量为106CFU/m L,原油浓度为0.5%(W/V),振荡培养7 d时,降解率可达62.90%。GC-MS分析表明:石油中长链烷烃C15~C21被完全降解,C22~C32部分降解,降解方式为单末端氧化。展开更多
基金supported by the National Natural Science Foundation of China (Nos. 41877378 and 41425016)the China Postdoctoral Science Foundation (No. 2021M691924)the Shandong Province Natural Science Foundation of Major Basic Research Program, China (No. ZR2020ZD34)。
文摘Shewanella sp. ANA-3 with the respiratory arsenate reductase(ArrAB) and MR-1 with ferric reduction ability always coexist in the presence of high arsenic(As)-containing waste residue. However, their synergistic impacts on As transformation and mobility remain unclear. To identify which bacterium, ANA-3 or MR-1, dominates As mobility in the coexisting environment, we explored the As biotransformation in the industrial waste residue in the presence of Shewanella sp. ANA-3 and MR-1. The incubation results show that As(Ⅲ)was the main soluble species, and strain ANA-3 dominated As mobilization. The impact of ANA-3 was weakened by MR-1, probably due to the survival competition between these two bacteria. The results of micro X-ray fluorescence and X-ray photoelectron spectroscopy analyses further reveal the pathway for ANA-3 to enhance As mobility. Strain ANA-3 almost reduced 100% surface-bound Fe(Ⅲ), and consequently led to As(V) release. The dissolved As(V) was then reduced to As(Ⅲ) by ANA-3. The results of this study help to understand the fate of arsenic in the subsurface and highlight the importance of the safe disposal of high As-containing industrial waste.
文摘分离得到1株对染料活性艳红X-3B具有高效降解作用的菌株,经生理生化鉴定结合16S r DNA分析鉴定为希瓦氏菌属,命名为Shewanella sp.3Y。将该菌固定到活性炭上构建了生物活性炭小试装置,用其处理染料活性艳红X-3B及与其结构类似的7种活性染料模拟废水时,水力停留时间2 h,对模拟染料废水COD去除率均在90%以上,对色度去除率在70%以上,大部分达到90%以上,而对UV_(254)的去除率差异较大。
文摘从患病拟穴青蟹(Scylla paramamosain)肝胰腺中分离到2种优势菌株,对2种优势菌株进行了鉴定和特性分析。利用形态学观察、生理生化特性分析和16S r DNA序列测定法对细菌类别进行判定,并采用药敏试验对细菌的耐药性进行分析。结果表明,这2种细菌分别为弧菌(Vibrio sp.)和希瓦氏菌(Shewanella),利用15种抗生素对2种细菌进行药敏试验分析,发现2种细菌对恩诺沙星、氟苯尼考、强力霉素高度敏感,对其他几种抗生素则有一定的耐受性。研究进一步证实了海水致病性弧菌是拟穴青蟹细菌性疾病高发的主要原因,也表明希瓦氏菌可能是一种潜在病原菌,与弧菌协同作用,对拟穴青蟹规模化养殖造成危害,同时也为拟穴青蟹养殖过程中细菌性病害的确定和防治提供参考依据。
文摘鉴于弗氏柠檬酸杆菌和迟钝爱德华氏菌是"人-兽-鱼"共患性条件致病菌,因此利用微生物拮抗原理研发微生态制剂进行细菌病害的防治对环境具有友好性.分别以从患病中华鳖分离的弗氏柠檬酸杆菌和迟钝爱德华氏菌作为指示菌,初步筛选获得对这2种菌具有拮抗作用的菌株6-1-1M和7-1-1M.用点种法和纸片法进一步验证了其拮抗活性,测定了2株菌930 bp的16S r DNA基因片段序列,发现二者序列完全一致,系统进化分析表明其属于希瓦氏菌属(Shewanella sp.).安全性验证实验结果表明其无致病性,具有开发应用前景.
