Correlation between Egg Production Traits and Broodiness Traits in Shiqiza,Langya and Luqin B2 Chickens

[ Objective] To investigate the relationship between the egg production traits and broodiness of Shiqiza, Langya and Luqin B2 chickens. E Method] Three chicken breeds, Shiqiza chickens （a breed preserved by Shandong Academy of Agricultural Sciences）, Langya chickens （a local breed in Shandong Province） and Luqin B2 chickens （a cultivated breed） were selected. Then their broodiness traits were observed and the egg productions were analyzed. Finally, the relationships between the egg production traits and broodiness were analyzed by SAS software. [ Result] The Shiqiza, Langya and Luqin B2. chickens were broody and their broodiness rate was 15.80%, 2.24% and 6.95%, respectively. Compared with the non-broody chickens, the egg productions of Shiqiza, Langya and Luqin B2 chickens were respectively decreased by 17.16%, 1.53% and 10.8% at the age of 43 weeks. The three breeds had significant differences in repeats of broody cycles （ P 〈 0.05）, and extremely significant differences in the broodiness duration, the the first day of broodiness, the age at the first egg, total amount of abnormal eggs and amount of double- yolked eggs （P 〈 0.01 ）. [Conclusion] The broodiness is a main factor affecting egg production traits. The degree of effects of broodiness on egg production traits is decreased successively in Shiqiza chicken, Luqin B2 chicken and Langya chicken.

应用血液蛋白质多态性探讨石歧杂鸡的起源和分化

本文测定了与石岐杂鸡有关的８个家鸡品种（群体）的血液碱性磷酸酶（Ａｋｐ－１及Ａｋｐ－２）、淀粉酶（Ａｍｙ－１及Ａｍｙ－２）、酯酶（Ｅｓ－１）和血红蛋白（Ｈｂ－１）多态性，用主成分分析的方法研究了石峻杂鸡的起源与分化，结果表明，石岐杂鸡主要受石岐鸡和白洛克鸡血液的影响，而与杏花鸡、清远麻鸡等也存在一定的关系，并且可能正是这种关系构成了石歧杂鸡广泛的进传基础：既保持与外种鸡相仿的生长速度与体重，又具备本地地肉质优美的特性。结果还表明了石岐杂类鸡本身的分化。

石歧杂、琅琊鸡和鲁禽B2产蛋和就巢性状的研究

[目的]研究石歧杂、琅琊鸡和鲁禽B2系就巢性和产蛋性能的关系。[方法]对山东省农业科学院家禽保存品种石歧杂、山东地方品种琅琊鸡和培育品系鲁禽B2系进行就巢表型观察和产蛋性能统计,利用SAS软件分析就巢性和产蛋性能的关系。[结果]3个品种都有不同程度的就巢表现,就巢率分别为15.8%、2.24%和6.95%;3个品种就巢鸡43周产蛋量比正常鸡分别减少17.16%、1.53%和10.8%;3个品种间就巢次数存在显著差异(P<0.05),就巢持续时间、首次就巢日龄、开产日龄、总畸形蛋数和双黄蛋数均存在极显著差异(P<0.01)。[结论]就巢性是影响产蛋性能的主要因素,3个品种中就巢性影响产蛋性能的程度从高到低依次为石歧杂,鲁禽B2和琅琊鸡。

石歧杂鸡催乳素基因外显子4变异与产蛋就巢性状的关联分析

利用PCR-SSCP技术和测序技术对催乳素(prolactin,PRL)基因外显子4进行SNP检测,并分析了该基因与石歧杂鸡产蛋、就巢性状的相关性。结果表明,PRL基因外显子4存在1个多态性位点,该位点在6017位点发生G→A的碱基突变,产生了AA、AB和BB 3种基因型,AA基因型为优势基因型,等位基因A为优势等位基因,该突变未导致氨基酸的变异;该位点的变异与开产日龄、首次就巢日龄呈现一定的显著差异趋势,BB基因型开产日龄、首次就巢日龄都晚于AA和AB基因型。

石歧杂鸡胚胎成纤维细胞的体外培养

用组织块法对石歧杂鸡胚胎成纤维细胞进行原代和传代培养,探讨禽类胚胎成纤维细胞适宜的培养模式。成功获得较均一的稳定的细胞群体,并成功地对细胞进行了冷冻保存和复苏。对于进行大规模的动物细胞培养具有重要的指导意义。