Objective To analyze the genetic polymorphism of 5 STR loci (D12S304, D12S313, D12S1583, D12S1640 and D12S1708) on chromosome 12 in Chinese Han population. Methods EDTA-anticoagulated blood specimens were collected fr...Objective To analyze the genetic polymorphism of 5 STR loci (D12S304, D12S313, D12S1583, D12S1640 and D12S1708) on chromosome 12 in Chinese Han population. Methods EDTA-anticoagulated blood specimens were collected from unrelated individuals of Chinese Han population in Shaanxi province. DNA samples were extracted with the Wizard Genomic DNA purification Kit and were amplified by polymerase chain reaction (PCR) technique. The PCR products were analyzed by ABI 3100 Genetic Analyzer. Results All 5 loci were in Hardy-Weinberg equilibrium. Allele and genotype frequencies, heterozygosity, power of discrimination, polymorphism information content, probability of paternity exclusion and matching probability of each locus were calculated in Excel 2002. Conclusion They are complex loci with lots of evenly distributed alleles and high heterozygosity in Chinese Han population. Thus they are informative polymorphic loci and valuable DNA marker which represents a superior alternative to many established STRs.展开更多
目的对湖南汉族人群20个常染色体短串联重复序列(short tandem repeat,STR)基因座的等位基因分布、群体遗传学参数和邻近群体的遗传分析进行研究,评估其在法医学中的应用价值。方法应用Power-Plex21^(■) 试剂盒对2997例湖南汉族无关个...目的对湖南汉族人群20个常染色体短串联重复序列(short tandem repeat,STR)基因座的等位基因分布、群体遗传学参数和邻近群体的遗传分析进行研究,评估其在法医学中的应用价值。方法应用Power-Plex21^(■) 试剂盒对2997例湖南汉族无关个体进行20个STR基因座复合扩增及等位基因分型,统计等位基因频率及群体遗传学参数,计算湖南汉族与已公开报道的13个群体间的Nei’s遗传距离,进行多维尺度分析并构建系统发生树。结果20个常染色体STR基因座的杂合度为0.6009~0.9116,个人识别能力为0.7745~0.9866,三联体非父排除率为0.2920~0.8191,二联体非父排除率为0.1910~0.7086,多态信息含量为0.5348~0.9093,基因型分布符合Hardy-Weinberg平衡。20个基因座的累积个体识别率为1-1.3509×10^(-21),三联体非父累积排除率与二联体非父累积排除率分别为0.999996523945999、0.999999996129773,基于湖南汉族人群与其他13个群体间遗传距离获得的多维尺度分析及系统发生树结果显示,其与湖北汉族相距较近,而与云南苗族最远。结论20个常染色体STR基因座在湖南汉族人群中呈高度多态性和良好的鉴别能力,能为该地区法医学个体识别、亲权鉴定和群体学研究提供基础数据。展开更多
文摘Objective To analyze the genetic polymorphism of 5 STR loci (D12S304, D12S313, D12S1583, D12S1640 and D12S1708) on chromosome 12 in Chinese Han population. Methods EDTA-anticoagulated blood specimens were collected from unrelated individuals of Chinese Han population in Shaanxi province. DNA samples were extracted with the Wizard Genomic DNA purification Kit and were amplified by polymerase chain reaction (PCR) technique. The PCR products were analyzed by ABI 3100 Genetic Analyzer. Results All 5 loci were in Hardy-Weinberg equilibrium. Allele and genotype frequencies, heterozygosity, power of discrimination, polymorphism information content, probability of paternity exclusion and matching probability of each locus were calculated in Excel 2002. Conclusion They are complex loci with lots of evenly distributed alleles and high heterozygosity in Chinese Han population. Thus they are informative polymorphic loci and valuable DNA marker which represents a superior alternative to many established STRs.
文摘目的对湖南汉族人群20个常染色体短串联重复序列(short tandem repeat,STR)基因座的等位基因分布、群体遗传学参数和邻近群体的遗传分析进行研究,评估其在法医学中的应用价值。方法应用Power-Plex21^(■) 试剂盒对2997例湖南汉族无关个体进行20个STR基因座复合扩增及等位基因分型,统计等位基因频率及群体遗传学参数,计算湖南汉族与已公开报道的13个群体间的Nei’s遗传距离,进行多维尺度分析并构建系统发生树。结果20个常染色体STR基因座的杂合度为0.6009~0.9116,个人识别能力为0.7745~0.9866,三联体非父排除率为0.2920~0.8191,二联体非父排除率为0.1910~0.7086,多态信息含量为0.5348~0.9093,基因型分布符合Hardy-Weinberg平衡。20个基因座的累积个体识别率为1-1.3509×10^(-21),三联体非父累积排除率与二联体非父累积排除率分别为0.999996523945999、0.999999996129773,基于湖南汉族人群与其他13个群体间遗传距离获得的多维尺度分析及系统发生树结果显示,其与湖北汉族相距较近,而与云南苗族最远。结论20个常染色体STR基因座在湖南汉族人群中呈高度多态性和良好的鉴别能力,能为该地区法医学个体识别、亲权鉴定和群体学研究提供基础数据。
