High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the sa...High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases.We thus collected bile,stool,saliva,plaque,sputum,and conjunctival swab samples and performed DNA extraction with three commercial kits.For each combination of the specimen type and DNA extraction kit,20-gigabase(Gb)metagenomic data were generated using short-read sequencing.While profiles of the specimen types showed close proximity to each other,we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits.No kit outperformed all selected kits on every specimen.We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit.Depending on the specimen,our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution,but DNA-based identification is superior to identification by mass spectrometry.Finally,longread nanopore sequencing confirmed the results(correlation coefficient>0.98).Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients.展开更多
梳理数智时代可应用在阅读服务领域的信息技术,调研国内外近3年与新一代信息技术环境下的阅读服务相关的研究成果,为该领域的研究发展提供参考。利用CNKI和Web of Science数据库分别对2019—2021年国内外的相关研究成果进行检索,经过筛...梳理数智时代可应用在阅读服务领域的信息技术,调研国内外近3年与新一代信息技术环境下的阅读服务相关的研究成果,为该领域的研究发展提供参考。利用CNKI和Web of Science数据库分别对2019—2021年国内外的相关研究成果进行检索,经过筛选获得目标文献294篇,对其期刊来源和研究方法进行统计,并对研究主题进行分析与总结。总结出数智时代国内外阅读服务研究主题主要包括新一代信息技术环境下的阅读服务系统与平台、阅读服务内容与策略、阅读服务用户、阅读服务评价和阅读服务管理等5个主题,在此基础上对国内外研究方向异同进行分析,并展望领域内未来的研究方向。展开更多
文摘High-quality DNA extraction is a crucial step in metagenomic studies.Bias by different isolation kits impairs the comparison across datasets.A trending topic is,however,the analysis of multiple metagenomes from the same patients to draw a holistic picture of microbiota associated with diseases.We thus collected bile,stool,saliva,plaque,sputum,and conjunctival swab samples and performed DNA extraction with three commercial kits.For each combination of the specimen type and DNA extraction kit,20-gigabase(Gb)metagenomic data were generated using short-read sequencing.While profiles of the specimen types showed close proximity to each other,we observed notable differences in the alpha diversity and composition of the microbiota depending on the DNA extraction kits.No kit outperformed all selected kits on every specimen.We reached consistently good results using the Qiagen QiAamp DNA Microbiome Kit.Depending on the specimen,our data indicate that over 10 Gb of sequencing data are required to achieve sufficient resolution,but DNA-based identification is superior to identification by mass spectrometry.Finally,longread nanopore sequencing confirmed the results(correlation coefficient>0.98).Our results thus suggest using a strategy with only one kit for studies aiming for a direct comparison of multiple microbiotas from the same patients.
文摘梳理数智时代可应用在阅读服务领域的信息技术,调研国内外近3年与新一代信息技术环境下的阅读服务相关的研究成果,为该领域的研究发展提供参考。利用CNKI和Web of Science数据库分别对2019—2021年国内外的相关研究成果进行检索,经过筛选获得目标文献294篇,对其期刊来源和研究方法进行统计,并对研究主题进行分析与总结。总结出数智时代国内外阅读服务研究主题主要包括新一代信息技术环境下的阅读服务系统与平台、阅读服务内容与策略、阅读服务用户、阅读服务评价和阅读服务管理等5个主题,在此基础上对国内外研究方向异同进行分析,并展望领域内未来的研究方向。
