Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension...Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.展开更多
The cross-well acoustic tomography as an advanced technique in 1980's is widely researchedand improved now. It is found that the velocity of oil-layer is lower than that Of country rock byabout ten percent accordi...The cross-well acoustic tomography as an advanced technique in 1980's is widely researchedand improved now. It is found that the velocity of oil-layer is lower than that Of country rock byabout ten percent according to some reports abroad. It had been tested to drive oil by steam inoil--field. In China the first test of cross-well acoustic tomography was performed in Fuyu oilfield, Jinn Province, in Dec. 1992. Through data processing in detail, an acoustic tomogram wasobtained, in which the distribution and inclination of oil layers between two boreholes are well revealed and coincident with the logging results. SOme technical problems existed in this experiment are POinted.展开更多
[Objective] The study aimed at analyzing the dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation based on 3S technology. [Method] At semi-arid and arid areas in t...[Objective] The study aimed at analyzing the dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation based on 3S technology. [Method] At semi-arid and arid areas in the west of Jilin Province, water resource balance between the amount of oil-field water supply and ecological water requirement in the constructed wetland irrigated by oil-field water during 2001-2010 was investigated firstly. Afterwards, based on 3S technology, the partition and dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation in 2001, 2006, 2008 and 2010 were analyzed. [Result] The annual ecological water requirement of the constructed wetland from 2003 to 2010 varied from 1.62×106 to 2.24×106 m3, and the annual amount of oil-field water supply in the region changed from 2.12×106 to 2.84×106 m3, which showed that the supply amount of oil-field water could meet the basic ecological water requirement of the constructed wetland. Meanwhile, compared with 2001, the areas of water region and paddy field in 2010 increased by 2.3 and 10.0 times, and the areas of forest and marsh rose by 40.15% and 29.5.0% respectively. [Conclusion] Water shortage and ecological environment problem of arid and semi-arid areas had been improved by oil-field water irrigation.展开更多
This paper introduces the application and development of welding technology in the oil-field construction in China. It focuses on introducing,by statistics,the growth of working capacity in the welding engineering of ...This paper introduces the application and development of welding technology in the oil-field construction in China. It focuses on introducing,by statistics,the growth of working capacity in the welding engineering of oil well heads,long-distance oil transmission lines,large-scale storage tanks, various kinds of oil station and stores, and the applications of welding technology in the typical construction of oil field. Finally, it presents suggestions on the future development of welding technology in oil-field construction for discussion.展开更多
文摘Seventy-nine strains of bioflocculant-producing bacteria were isolated from 3 activated sludge samples. Among them, strain MYC was found to have the highest and stable flocculating rate for both kaolin clay suspension and oil-field produced water. The bacterial strain was identified as Klebsiella sp. MYC according to its morphological and biochemical characteristics and 16SrDNA sequence. The optimal medium for bioflocculant production by this bacterial strain was composed of cane sugar 20 g L^-1, KH2PO4 2 g L^-1, K2HPO4 5 g L^- 1, （ NH4）2SO4 0.2 g L^-1, urea 0.5 g L^- 1 and yeast extract 0.5 g L^- 1, the initial pH being 5.5. When the suspension of kaolin clay was treated with0.5% of Klebsiella sp. MYC culture broth, the flocculating rate reached more than 90.0% in the presence of 500mg L^-1 CaCI2, while the flocculating rate for oil-field produced water was near 80.0% in a pH range of 7.0 - 9.0 with the separation of oil and suspended particles from the oil-field produced water under similar conditions. The environment-friendly nature of the bioflocculant and high flocculating rate of the strain make the bioflocculant produced by Klebsiella sp. MYC an attractive bioflocculant in oil-field produced water treatment.
文摘The cross-well acoustic tomography as an advanced technique in 1980's is widely researchedand improved now. It is found that the velocity of oil-layer is lower than that Of country rock byabout ten percent according to some reports abroad. It had been tested to drive oil by steam inoil--field. In China the first test of cross-well acoustic tomography was performed in Fuyu oilfield, Jinn Province, in Dec. 1992. Through data processing in detail, an acoustic tomogram wasobtained, in which the distribution and inclination of oil layers between two boreholes are well revealed and coincident with the logging results. SOme technical problems existed in this experiment are POinted.
基金Supported by 2007 Environmental Protection Project of Jilin Provincial Department of Environmental Protection(2007-09)
文摘[Objective] The study aimed at analyzing the dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation based on 3S technology. [Method] At semi-arid and arid areas in the west of Jilin Province, water resource balance between the amount of oil-field water supply and ecological water requirement in the constructed wetland irrigated by oil-field water during 2001-2010 was investigated firstly. Afterwards, based on 3S technology, the partition and dynamic variation of land-use types of the constructed wetland before and after oil-field water irrigation in 2001, 2006, 2008 and 2010 were analyzed. [Result] The annual ecological water requirement of the constructed wetland from 2003 to 2010 varied from 1.62×106 to 2.24×106 m3, and the annual amount of oil-field water supply in the region changed from 2.12×106 to 2.84×106 m3, which showed that the supply amount of oil-field water could meet the basic ecological water requirement of the constructed wetland. Meanwhile, compared with 2001, the areas of water region and paddy field in 2010 increased by 2.3 and 10.0 times, and the areas of forest and marsh rose by 40.15% and 29.5.0% respectively. [Conclusion] Water shortage and ecological environment problem of arid and semi-arid areas had been improved by oil-field water irrigation.
文摘This paper introduces the application and development of welding technology in the oil-field construction in China. It focuses on introducing,by statistics,the growth of working capacity in the welding engineering of oil well heads,long-distance oil transmission lines,large-scale storage tanks, various kinds of oil station and stores, and the applications of welding technology in the typical construction of oil field. Finally, it presents suggestions on the future development of welding technology in oil-field construction for discussion.
文摘目的 观察双合汤联合关节腔药物注射治疗膝关节骨性关节炎临床观察及对肿瘤坏死因子-α(Tumor necrosis factor alpha, TNF-α)的影响。方法 研究总计纳入膝关节骨性关节炎患者150例(2022年7月—2023年3月门诊及住院患者),以随机数字表法将纳入患者分为3组,治疗组(A组)患者采取双合汤联合玻璃酸钠关节腔注射治疗,西药对照组(B组)患者采取玻璃酸钠关节腔注射治疗,中药对照组(C组)患者采取双合汤治疗,各组50例,3组患者均治疗5周时间,治疗过程中A组脱落2例,B组脱落1例,C组未见脱落。观察比较3组患者临床治疗效果、治疗前后中医证候(关节肿胀僵硬、活动受限、时常口干等)积分变化、治疗前后3组患者视觉模拟疼痛评分法(Visual Analogue Scale, VAS)变化、西安大略和麦克马斯特大学骨关节炎指数量表(Western Ontario and McMaster Universities Osteoarthritis Index, WOMAC)评分变化、肿瘤坏死因子-α(Tumor necrosis factor alpha, TNF-α)水平变化、美国特种外科医院膝关节评分(hospital for special surgery knee score, HSS评分)变化、健康状况调查简表(MOS item short from health survey, SF-36)评分变化、治疗不良反应情况。结果 A组患者治疗总有效率高于B组与C组患者,P<0.05;B组与C组患者治疗总有效率比较差异无统计学意义,P>0.05;治疗前3组患者中医证候(关节肿胀僵硬、活动受限、时常口干等)积分、VAS评分、WOMAC评分、TNF-α水平、HSS评分与SF-36评分比较,P>0.05;较治疗前,治疗后3组患者中医证候(关节肿胀僵硬、活动受限、时常口干等)积分、VAS评分、WOMAC评分、TNF-α水平、HSS评分以及SF-36评分等指标均改善,P<0.05;A组患者经治疗后中医证候(关节肿胀僵硬及活动受限、时常口干等)积分、VAS评分、WOMAC评分、TNF-α水平、HSS评分与SF-36评分均明显优于B组与C组,C组患者治疗后中医证候(关节肿胀僵硬、活动受限、时常口干等)积分、VAS评分、WOMAC评分、TNF-α水平、HSS评分与SF-36评分与B组患者相当,P>0.05;3组患者治疗不良反应率均较低,且不良反应症状轻微,可自行缓解,P>0.05。结论 双合汤联合关节腔药物注射治疗膝关节骨性关节炎效果佳,可一定程度降低TNF-α水平,改善患者病情,提升患者膝关节功能及生活质量,治疗不良反应少,治疗安全可靠。
