Objective: To investigate the effect of prepared rhubarb on insulin resistance in patients with pregnancy induced hypertension (PIH) and its mechanism. Methods: All the 92 patients accepted 75 g oral glucose tolerance...Objective: To investigate the effect of prepared rhubarb on insulin resistance in patients with pregnancy induced hypertension (PIH) and its mechanism. Methods: All the 92 patients accepted 75 g oral glucose tolerance test (OGTT) and insulin release test before and after treatment. These patients were divided into two groups (treated group and control group). Prepared rhubarb and nifedipine were given to the treated group, while nifedipine was given to the control group alone. Circulating endothelial cell (CEC), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) were measured and glucose area under curve (GAUC), insulin area under curve (IAUC), insulin sensitivity index (ISI) were calculated. And 30 normal pregnant women were selected as the healthy group. Results: CEC, TNF-a, IL-6, GAUC and IAUC of PIH patients were significantly higher than those of the normal healthy group; while ISI of PIH patients was significantly lower than that of the healthy group. ISI was significantly negatively correlated to CEC, TNF-a and IL-6. After treatment, CEC, TNF-α, IL-6, GAUC decreased and ISI increased significantly in the treated group; while in the control group, all above-mentioned parameters showed no change. Conclusion: Prepared rhubarb can improve insulin resistance of PIH by reducing vascular endothelial cell's damage.展开更多
目的建立5种大黄制剂(保肾片、新保肾片、肾炎宁片、新肾炎胶囊、炎黄保肾胶囊)的微生物限度检查方法。方法按照《中国药典》(2010年版)微生物限度检查法的规定,采用常规法、培养基稀释法、薄膜过滤法对5种大黄制剂的细菌、霉菌和酵母...目的建立5种大黄制剂(保肾片、新保肾片、肾炎宁片、新肾炎胶囊、炎黄保肾胶囊)的微生物限度检查方法。方法按照《中国药典》(2010年版)微生物限度检查法的规定,采用常规法、培养基稀释法、薄膜过滤法对5种大黄制剂的细菌、霉菌和酵母菌进行回收率测定,通过回收率试验测定制剂的抑菌性;采用直接接种法对制剂控制菌进行测定。结果 5种大黄制剂均有抑菌作用,均不可用常规法进行细菌、霉菌和酵母菌测定;2种大黄制剂(保肾片、新保肾片)对枯草芽孢杆菌有较强的抑菌作用,可采用培养基稀释法(0.2 m L/皿)进行细菌数测定,霉菌和酵母菌可采用常规法检查;1种大黄制剂(肾炎宁片)对枯草芽孢杆菌和白色念珠菌有较强的抑菌作用,可采用培养基稀释法(0.2 m L/皿)进行细菌、霉菌和酵母菌数测定;2种大黄制剂(新肾炎胶囊、炎黄保肾胶囊)对枯草芽孢杆菌和金黄色葡萄球菌有很强的抑菌作用,需采用薄膜过滤法对细菌数进行测定,对白色念珠菌有较强的抑菌作用,可采用培养基稀释法(0.2 m L/皿)进行霉菌和酵母菌数测定。结论大黄具有明显的抑菌性,严格按照《中国药典》(2010年版)规定,建立合理适用的5种大黄制剂微生物限度检查方法,以有效控制其质量。展开更多
文摘Objective: To investigate the effect of prepared rhubarb on insulin resistance in patients with pregnancy induced hypertension (PIH) and its mechanism. Methods: All the 92 patients accepted 75 g oral glucose tolerance test (OGTT) and insulin release test before and after treatment. These patients were divided into two groups (treated group and control group). Prepared rhubarb and nifedipine were given to the treated group, while nifedipine was given to the control group alone. Circulating endothelial cell (CEC), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) were measured and glucose area under curve (GAUC), insulin area under curve (IAUC), insulin sensitivity index (ISI) were calculated. And 30 normal pregnant women were selected as the healthy group. Results: CEC, TNF-a, IL-6, GAUC and IAUC of PIH patients were significantly higher than those of the normal healthy group; while ISI of PIH patients was significantly lower than that of the healthy group. ISI was significantly negatively correlated to CEC, TNF-a and IL-6. After treatment, CEC, TNF-α, IL-6, GAUC decreased and ISI increased significantly in the treated group; while in the control group, all above-mentioned parameters showed no change. Conclusion: Prepared rhubarb can improve insulin resistance of PIH by reducing vascular endothelial cell's damage.
文摘目的建立5种大黄制剂(保肾片、新保肾片、肾炎宁片、新肾炎胶囊、炎黄保肾胶囊)的微生物限度检查方法。方法按照《中国药典》(2010年版)微生物限度检查法的规定,采用常规法、培养基稀释法、薄膜过滤法对5种大黄制剂的细菌、霉菌和酵母菌进行回收率测定,通过回收率试验测定制剂的抑菌性;采用直接接种法对制剂控制菌进行测定。结果 5种大黄制剂均有抑菌作用,均不可用常规法进行细菌、霉菌和酵母菌测定;2种大黄制剂(保肾片、新保肾片)对枯草芽孢杆菌有较强的抑菌作用,可采用培养基稀释法(0.2 m L/皿)进行细菌数测定,霉菌和酵母菌可采用常规法检查;1种大黄制剂(肾炎宁片)对枯草芽孢杆菌和白色念珠菌有较强的抑菌作用,可采用培养基稀释法(0.2 m L/皿)进行细菌、霉菌和酵母菌数测定;2种大黄制剂(新肾炎胶囊、炎黄保肾胶囊)对枯草芽孢杆菌和金黄色葡萄球菌有很强的抑菌作用,需采用薄膜过滤法对细菌数进行测定,对白色念珠菌有较强的抑菌作用,可采用培养基稀释法(0.2 m L/皿)进行霉菌和酵母菌数测定。结论大黄具有明显的抑菌性,严格按照《中国药典》(2010年版)规定,建立合理适用的5种大黄制剂微生物限度检查方法,以有效控制其质量。
