Study on the anesthestic quality of intravenous infusion of shumianning Ⅱ in minipigs

Pigs are suitable experimental animals for many surgical techniques[1-4]. Shumianning Ⅱ is compound anesthetic which cantain ketamine,xylazine and midazolam and can be only used for swine anesthesia. The purpose of this study was to evaluate the effects of Shumianning Ⅱ administered by continuous rate infusion using microinjection pump[5]on anesthetic quality,cardiopulmonary function and biochemistrical values in minipigs.The anesthestic quality of shumianning Ⅱ was investigated in 6 Bama minipigs,aged 8. 7 ± 0. 2 mon,mean weight 23. 7 ± 5. 0 kg. Minipigs were induced with shumianning Ⅱ 0. 03 mL/kg from the ear vein,then received with constant rate infusions of shumianning Ⅱ 0. 03 mL/kg·h for 1h by microinjection pump. Heart rate (HR),respiratory rate (RR),rectal temperature (RT),non-invasive arterial blood pressure (NABP),pulse oxygen saturation (SpO2) and end-tidal pressure of carbon dioxide (P ET CO2) were measured and the effectiveness of analgesia,sedation and muscle relaxation were observed every 5 min; the biochemical parameters of ALP,ALT,AST,CREA,UREA,GLU and TP were measured before and 1 h,24 h,48 h,72 h,168 h after the administration of Shumianning Ⅱ; 5 mL blood sample from the precaval vein was collected at 1 min,3 min,5 min,10 min,25 min,40 min,45 min,60 min,70 min,80min,90 min and 100 min,respectively,then the plasma concentrations of ketamine,xylazine and midazolam were measured with high-performance liquid chromatography.The results showed that the status of HR,RR,RT,NABP,SpO2and P ET CO2were significantly different from the base value at some time points (P【0. 05),but all parameters were in the normal physiologic range. Levels of SpO2 at1 min and 5 min (P【0. 05) were significantly lower than the normal physiologic range. Cardiovascular and blood chemistry effects was small,only the level of GLU was higher than the base value (P【0. 05),while others were all not significantly different.In the period of intravenous infusion of Shumianning Ⅱ,the plasma concentrations of ketamine,midazolam and xylazine were downtrend in first 3 ~ 10 min,and began to raise at 14 min,the value reach the top at 20 ~ 55 min,the plasma concentration can be stable for about 1 h.Induction anesthesia in minipigs was quick,the stage of surgical anesthesia was long and smooth,recovery was smooth and quick. There was no pain reaction (eg. emesis,apnea,salivation) on every stage in the experiments. Using microinjection pump can be inexpensive,easier to perform. shumianningⅡmeets clinical operation requirement.

Shumian capsule(舒眠胶囊) improves symptoms of sleep mood disorder in convalescent patients of Corona Virus Disease 2019

OBJECTIVE:To evaluate the clinical effectiveness of Shumian capsule(舒眠胶囊)in improving the symptoms of insomnia,anxiety,depression,and other symptoms of convalescent patients of COVID-19.METHODS:Totally 200 patients were collected and randomly divided into experiment group(n=100)and control group(n=100).The control group was treated with Shumian capsule simulator,and the experiment group was treated with Shumian capsule.The improvement of TCM symptom score,the total effective rate and symptom disappearance rate of TCM symptoms in the two groups before and after treatment were observed,and the clinical effect was evaluated.RESULTS:One week after treatment,the scores of anxiety symptoms in the experiment group were significantly different from those in the control group(P<0.05),but there was no significant difference in the scores of insomnia and depression between the experiment group and the control group(P>0.05).There was no significant difference in the total effective rate and disappearance rate of TCM symptoms of insomnia,anxiety and depression between the experiment group and the control group(P>0.05).After 2 weeks of treatment,the scores of insomnia,anxiety,depression and the total effective rate of TCM symptoms in the experiment group were significantly different from those in the control group(P<0.05).There was no significant difference in the disappearance rate of insomnia,anxiety and depression between the experiment group and the control group(P>0.05).There were no significant differences in heart rate,respiration,systolic blood pressure and diastolic blood pressure between the experiment group and the control group(P>0.05).CONCLUSION:Shumian capsule can significantly improve the symptoms of insomnia,anxiety and depression in COVID-19’s convalescent patients with sleep and mood disorders.