Objective Shunaoxin Dropping Pills(SDPs),a Chinese patent medicine,has been used widely in China for the treatment of headache,amnesia,and insomnia.The aim of the present study is to observe the effect of SDPs on indu...Objective Shunaoxin Dropping Pills(SDPs),a Chinese patent medicine,has been used widely in China for the treatment of headache,amnesia,and insomnia.The aim of the present study is to observe the effect of SDPs on inducing angiogenesis and neurogenesis in vitro.Methods The present testing system using the serum obtained from animals ig treated with SDPs and a co-culture system in vitro was used to investigate if SDPs promotes brain microvascular endothelial cells(BMECs)tube formation and neural differentiation of neural stem/progenitor cells(NSPCs),which plays important roles in angiogenesis and neurogenesis.Results The SDPs serum sampled from rats ig treated with SDPs for 3 d dose-dependently promoted the tube like structure formation of cultured BMECs,and enhanced the fraction of MAP-2 positive cells of NSPCs,which co-cultured with the BMECs and astrocyte.In addition,there was no significant change in the percentage of glial fibrillary acidic protein positive cells.Conclusion Our results show that SDPs serum can induce neural differentiation and BMECs tube formation in vitro.展开更多
Objective: The main objective of the study is to screen the quality markers(Q-markers) for relieving oxidative stress damage and against chronic cerebral ischemia in Shunaoxin pills(SNX). Methods: The benefit effect o...Objective: The main objective of the study is to screen the quality markers(Q-markers) for relieving oxidative stress damage and against chronic cerebral ischemia in Shunaoxin pills(SNX). Methods: The benefit effect of SNX was evaluated by a rat chronic cerebral ischemia model. The main ingredients of SNX were identified by ultra-performance liquid chromatography-quadrupole time-of-flight, whereas its core targets and pathways around antioxidative stress were predicted by Pharm Mapper and kyoto encyclopedia of genes and genomes(KEGG) analysis. Moreover, the antioxidants were screened by high-performance liquid chromatography with postcolumn derivatization system and then representative ingredients were verified by cell experiments. Results: SNX could increase expression of catalase and superoxide dismutase(SOD) as well as antagonize oxidative damage in the brain. The effects may be related to three types of antioxidant pathways, including nitrogen metabolism, arachidonic acid metabolism, and the cyclic guanosine monophosphate-dependent protein kinase(c GMP-PKG) signaling pathway by multiple active components regulate targets. Among them, ferulic acid and ligustilide were shown the key scavenging ability for reactive oxygen free radicals and significantly increased the contents of nitric oxide(NO), NO synthase, and SOD as well as decreased malonaldehyde. Conclusion: The oxidation resistances of biological and chemical processes in SNX to protect against cerebral oxidative stress injury were preliminary revealed by an integrated network pharmacology and antioxidant activity-guided screen system. Ferulic acid and ligustilide played a major antioxidant role that could be used as Q-markers to control the quality of SNX.展开更多
基金Program for Changjiang Scholars and Innovative Research Team in University(PCSIR)National Science Foundation of China(30873395)+1 种基金Special Fund Project of Tianjin Science and Technology Innovation(08FDZDSH01405)Key Projects in the National Science and Technology Pillar Program(2007BAI47B01)
文摘Objective Shunaoxin Dropping Pills(SDPs),a Chinese patent medicine,has been used widely in China for the treatment of headache,amnesia,and insomnia.The aim of the present study is to observe the effect of SDPs on inducing angiogenesis and neurogenesis in vitro.Methods The present testing system using the serum obtained from animals ig treated with SDPs and a co-culture system in vitro was used to investigate if SDPs promotes brain microvascular endothelial cells(BMECs)tube formation and neural differentiation of neural stem/progenitor cells(NSPCs),which plays important roles in angiogenesis and neurogenesis.Results The SDPs serum sampled from rats ig treated with SDPs for 3 d dose-dependently promoted the tube like structure formation of cultured BMECs,and enhanced the fraction of MAP-2 positive cells of NSPCs,which co-cultured with the BMECs and astrocyte.In addition,there was no significant change in the percentage of glial fibrillary acidic protein positive cells.Conclusion Our results show that SDPs serum can induce neural differentiation and BMECs tube formation in vitro.
基金supported by a grant from the National Natural Science Foundation of China (No. 81673637, 81473403, 81673616 and 81430095)
文摘Objective: The main objective of the study is to screen the quality markers(Q-markers) for relieving oxidative stress damage and against chronic cerebral ischemia in Shunaoxin pills(SNX). Methods: The benefit effect of SNX was evaluated by a rat chronic cerebral ischemia model. The main ingredients of SNX were identified by ultra-performance liquid chromatography-quadrupole time-of-flight, whereas its core targets and pathways around antioxidative stress were predicted by Pharm Mapper and kyoto encyclopedia of genes and genomes(KEGG) analysis. Moreover, the antioxidants were screened by high-performance liquid chromatography with postcolumn derivatization system and then representative ingredients were verified by cell experiments. Results: SNX could increase expression of catalase and superoxide dismutase(SOD) as well as antagonize oxidative damage in the brain. The effects may be related to three types of antioxidant pathways, including nitrogen metabolism, arachidonic acid metabolism, and the cyclic guanosine monophosphate-dependent protein kinase(c GMP-PKG) signaling pathway by multiple active components regulate targets. Among them, ferulic acid and ligustilide were shown the key scavenging ability for reactive oxygen free radicals and significantly increased the contents of nitric oxide(NO), NO synthase, and SOD as well as decreased malonaldehyde. Conclusion: The oxidation resistances of biological and chemical processes in SNX to protect against cerebral oxidative stress injury were preliminary revealed by an integrated network pharmacology and antioxidant activity-guided screen system. Ferulic acid and ligustilide played a major antioxidant role that could be used as Q-markers to control the quality of SNX.
