全自动滴定仪在水质分析中的应用

介绍顺昕1800型全自动滴定仪的使用特点及在水质分析化学需要氧量(COD_(Cr))、高锰酸盐指数(COD_(Mn))、总硬度中的应用。进行化学需氧量(COD_(Cr))准确度测定,其低浓度、高浓度质控样品测定的相对误差分别为0.3%和0.7%,空白样品精密度良好,相对标准偏差(RSD)<1.0%,对实际水样高锰酸盐指数(COD_(Mn))和总硬度比对试验结果进行F检验和t检验,统计学评价表明手工滴定和全自动滴定结果无显著性差异。

Shunxin decoction(顺心组方) improves diastolic function in rats with heart failure with preserved ejection fraction induced by abdominal aorta constriction through cyclic guanosine monophosphate-dependent protein kinase Signaling Pathway

OBJECTIVE: To determine whether Shunxin decoction(顺心组方) improves diastolic function in rats with heart failure with preserved ejection fraction(HFp EF) by regulating the cyclic guanosine monophosphatedependent protein kinase(c GMP-PKG) signaling pathway. METHODS: Except for control group 8 and sham surgery group 8, the remaining 32 male Sprague-Dawlay rats were developed into HFp EF rat models using the abdominal aorta constriction method. These rats in the HFp EF model were randomly divided into the model group, the Shunxin high-dose group, the Shunxin lowdose group, and the Qiliqiangxin capsule group. The three groups received high-dose Shunxin decoction, lowdose Shunxin decoction, and Qiliqiangxin capsule by gavage, respectively, for 14 d. After the intervention, the diastolic function of each rat was evaluated by testing E/A, heart index, hematoxylin-eosin staining, Masson, myocardial ultrastructure, and N-terminal pro-brain natriuretic peptide(NT-pro BNP). The Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine(BATMAN-TCM) software was used to predict targets for which Shunxin decoction acts on the c GMP-PKG pathway. Natriuretic peptide receptor A(NPRA) and guanylate cyclase(GC) were detected by immunohistochemistry, and e NOS, phosphodiesterase 5A(PDE5A), and c GMP-dependent protein kinase 1(PKG I) were determined by Western blotting. RESULTS: Compared to the model group, the thickness of the interventricular septum at the end of diastole(IVSd) and the thickness of the posterior wall at the end of diastole(PWd) of the Shunxin decoction high-dose group, Shunxin decoction low-dose group, and Qiliqiangxin capsule group were all significantly reduced(P < 0.01). Furthermore, Shunxin decoction high-dose group E/A value was decreased(P < 0.01). Compared to the model group, the expression of NPRA and GC increased in the Shunxin decoction low-dose group and the Qiliqiangxin capsule group(P < 0.01). Compared to the model group, the expressions of e NOS and PKG I increased(P < 0.05) in the Shunxin decoction high-dose group. The expression of PDE5A expression decreased in the myocardium of the Shunxin decoction high-dose group, Shunxin decoction low-dose group, and Qiliqiangxin capsule group compared to the model group(P < 0.01). CONCLUSIONS: Shunxin decoction can improve diastolic function in rats with HFp EF. It increases the expression of NPRA, GC, and e NOS in the myocardial cell c GMP-PKG signaling pathway, upregulates c GMP expression, decreases PDE5A expression to reduce the c GMP degradation. Thus, the c GMP continually stimulates PKG I, reversing myocardial hypertrophy and improving myocardial compliance in HFp EF rats.

不同煎煮时间对解郁顺心汤中无机元素含量的影响

目的建立电感耦合等离子体原子发射光谱法（ICP-AES）测定解郁顺心汤中不同元素的方法,研究不同的煎煮时间对解郁顺心汤中元素煎出的影响。方法采用HNO3/H2O2微波消解制样,再应用ICP-AES法同时测定解郁顺心汤在煎煮时间为30、60、90min的水煎液中K、Ca、Na、Mg、Al、Zn、Fe、Sn、B、Li、Sr、Cd和As 13种元素的含量组成并进行分析。结果煎煮时间为30min时,解郁顺心汤中的各元素不能够完全析出,煎煮60min各元素基本煎出。解郁顺心汤中含有丰富的元素,其中常量元素中K元素的含量最高,微量元素中Zn元素含量最高。煎煮90min,未检测到Cd和As这2种元素。各元素的线性关系良好,r均大于0.999 0,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为95.07%～100.83%,RSD〈3%。结论为了减少煎煮时间且将解郁顺心汤中的各元素尽可能的煎出,煎煮时间应控制在60min。实验结果为解郁顺心汤物质基础和药效学研究提供一定依据。

DLC理论在工业旅游产品开发中的应用——以顺鑫农业股份有限公司工业旅游为例

西方发达国家工业旅游已有一百多年历史,已具有较成熟的经验。而我国工业旅游起步较晚,且主要以企业自发形成为主,缺少专业旅游组织的介入,旅游产品结构开发单一。文章结合DLC理论对旅游产品各阶段特征的分析,以顺鑫农业股份有限公司开发的工业旅游产品为例,分析工业旅游生命周期各阶段特征,探讨工业旅游产品开发原则,并提出相应的对策和建议。

太极拳中的养心心理学思想探析

太极拳是养心拳,其身心保健功能主要通过养心来实现。太极拳的养心思想是理论与方法的统一,是中国传统养生之道的实践与体现。太极拳的养心主要表现在静心、顺心、正心、和心四个方面,这些都是当今人们预防乃至消除疾患,维护身心健康的重要方法。