Objective:To Find the core targets and drug action mechanism of Si Miao decoction for the treatment of acute gouty arthritis.Methods:Through the TCMSP database,the chemical composition of all the drugs in Si Miao deco...Objective:To Find the core targets and drug action mechanism of Si Miao decoction for the treatment of acute gouty arthritis.Methods:Through the TCMSP database,the chemical composition of all the drugs in Si Miao decoction was obtained.The Perl script was compiled and the UniProt database was searched to determine the corresponding target of the chemical composition.Then,the disease databases such as OMIM,DisGeNET,and GeneCards were searched in order to determine acute gouty arthritis Related targets.Finally,screen common targets for drugs and diseases,use the STRING database and Cytoscape software to build a network control map of drugs-chemical components-targets-disease.Use R language software to screen common targets and find the four best The core targets of San for the treatment of acute gouty arthritis.The GO and KEGG analysis of the core targets were performed to clarify the core targets and mechanism of Si Miao decoction for the treatment of gout.Results:There are 64 effective chemical components in Si Miao decoction,197 genetic targets,600 disease targets,and 58 common targets.It is predicted that IL6,VEGFA,IL1B,JUN,PTGS2,and CCL2 may be treated by Si Miao decoction for gout The core target of arthritis.GO enrichment analysis identified 85 entries,of which biological processes mainly included the regulation of cytokine activity,protease activation,nucleic acid expression,etc.;KEGG pathway enrichment analysis identified 135 signaling pathways,of which signaling pathways involved the IL-17 signaling pathway,TNF signaling pathway,Th17 cell differentiation and other pathways.Conclusion:Si Miao decoction acts on acute gouty arthritis by regulating the occurrence of inflammation,and has significant anti-inflammatory and immune effects.The formula is rigorous and scientific,and it is worthy of clinical application.展开更多
目的 基于现代科学技术对四妙勇安汤进行改良,验证其促进糖尿病足伤口愈合的效果。方法 采用乙氧二羟丁酮辅助的单链DNA测序(kethoxal-assisted single stranded DNA sequencing, KAS-seq)技术检测糖尿病足患者创面组织和正常创面组织,...目的 基于现代科学技术对四妙勇安汤进行改良,验证其促进糖尿病足伤口愈合的效果。方法 采用乙氧二羟丁酮辅助的单链DNA测序(kethoxal-assisted single stranded DNA sequencing, KAS-seq)技术检测糖尿病足患者创面组织和正常创面组织,筛选糖尿病足相关的关键基因,并结合网络药理学,根据这些关键基因寻找有针对性的中药从而对四妙勇安汤进行改良,最后通过糖尿病足动物模型验证改良方的治疗效果。结果 通过临床样本测序分析得到CREB1、GRID2、PIK3R3、DRD2、ADORA1、TNFSF11、EDNRB、HTR1B 8个关键基因,靶向到黄柏、苍术、地黄和半夏4味中药配伍入原方。在灌胃干预第7天时改良方组(S+)小鼠伤口愈合效率显著高于对照组(Control)(P<0.05),第12天时S+组伤口愈合率显著高于原方组(S)(P<0.05)。改良方中药靶点在原方基础上更多富集到了神经系统、缺氧和血液循环调节相关通路中。结论 改良方与经方相比具有更好地促进糖尿病足伤口愈合的效果,相关机制可能与调节炎性反应、改善周围神经病变和促进伤口愈合等通路有关,同时也为中药方剂的临床加减应用提供了一种新方法和现代科学理论依据。展开更多
目的:观察凉血散瘀中药对糖尿病溃疡大鼠的干预作用,并从Wnt/β-catenin通路角度探讨其作用机制。方法:Wistar大鼠采用高脂高糖饲料联合小剂量链脲佐菌素(STZ)腹腔注射法制备糖尿病模型。分别取正常对照组与糖尿病组大鼠制作溃疡模型,...目的:观察凉血散瘀中药对糖尿病溃疡大鼠的干预作用,并从Wnt/β-catenin通路角度探讨其作用机制。方法:Wistar大鼠采用高脂高糖饲料联合小剂量链脲佐菌素(STZ)腹腔注射法制备糖尿病模型。分别取正常对照组与糖尿病组大鼠制作溃疡模型,并分为溃疡对照(CU)组、糖尿病溃疡(DU)组、四妙勇安汤组(DU-四妙组)、愈疡灵组(DU-愈疡灵组)、四妙勇安汤+愈疡灵组(DU-四妙+愈疡灵组),每组各15只。观察造模后第3、7、14 d各组创面愈合情况和织形态结构的变化的变化,采用ELISA法、RT-PCR法检测创面组织β-catenin、糖原合成激酶-3β(GSK-3β)蛋白及m RNA的表达水平。结果:DU组大鼠与CU组大鼠相比,第3、7、14 d的创面愈合率明显降低[(5.21±0.94)%vs(20.87±1.39)%,(31.70±1.21)%vs (49.99±1.58)%,(62.20±1.37)%vs (91.60±1.58)%, P <0.05];创面组织中β-catenin蛋白表达[(5.14±0.56) vs (9.04±0.65),(5.83±0.43) vs (10.08±0.35),(6.55±0.58) vs (12.77±0.30), P <0.05]及mRNA表达[(4.40±0.35) vs (6.92±0.16),(5.20±0.49) vs (7.12±0.48),(6.06±0.47) vs (9.24±0.55), P <0.05]均较低,而GSK-3β蛋白表达[(15.52±1.00) vs (7.04±0.53),(14.58±0.45) vs (6.05±0.23),(13.37±0.62) vs (4.49±0.37), P <0.05]及mRNA表达[(7.07±0.29) vs (3.55±0.32),(6.64±0.47) vs (3.00±0.28),(5.68±0.46) vs (1.98±0.19), P <0.05]则较高。与DU组大鼠相比,各治疗组大鼠各时间点的创面愈合率、创面组织中β-catenin蛋白及mRNA的表达水平均升高,而GSK-3β蛋白及mRNA的表达水平则降低,且治疗组中,以DU-四妙+愈疡灵组改善最明显。结论:具有凉血散瘀作用的四妙勇安汤及愈疡灵软膏均可通过干预Wnt/β-catenint通路的表达促进DU大鼠创面愈合,两者同用的效果优于单用其中一种。展开更多
目的分析四妙勇安汤的研究热点、研究趋势和处方配伍规律。方法纳入中国知识资源总库(CNKI)、万方数据知识服务平台V2.0(万方数据)、维普中文期刊服务平台(VIP)、中国生物医学文献数据库(SinoMed)、Pubmed、Web of Science(WOS)自建库至...目的分析四妙勇安汤的研究热点、研究趋势和处方配伍规律。方法纳入中国知识资源总库(CNKI)、万方数据知识服务平台V2.0(万方数据)、维普中文期刊服务平台(VIP)、中国生物医学文献数据库(SinoMed)、Pubmed、Web of Science(WOS)自建库至2022年12月31日的四妙勇安汤相关文献为研究对象,应用CiteSpace软件进行可视化分析,统计具体频次;以2006~2022年的四妙勇安汤验案建立处方数据库,形成药物共现复杂网络,并进行分析。结果共纳入文献1086篇,医学院校及其附属医院是研究主力,血栓闭塞性脉管炎、糖尿病足、冠心病、痛风性关节炎、类风湿关节炎是四妙勇安汤的主治病种,四妙勇安汤及其加减方对相关疾病的治疗是研究重点,基础研究主要关注该方的抗动脉粥样硬化相关机制。该方常配伍黄芪、地黄、赤芍、苍术、黄柏等中药。结论四妙勇安汤是中医研究的热点方剂之一,其临床适应证的拓展和处方用药配伍有一定规律可循,其药理作用机制还需深入研究。展开更多
基金National Natural Resources Foundation program(No.81373802)Beijing Natural Science Foundation(No.7172244)Beijing Science and Technology Project(No.Z191100006619024)
文摘Objective:To Find the core targets and drug action mechanism of Si Miao decoction for the treatment of acute gouty arthritis.Methods:Through the TCMSP database,the chemical composition of all the drugs in Si Miao decoction was obtained.The Perl script was compiled and the UniProt database was searched to determine the corresponding target of the chemical composition.Then,the disease databases such as OMIM,DisGeNET,and GeneCards were searched in order to determine acute gouty arthritis Related targets.Finally,screen common targets for drugs and diseases,use the STRING database and Cytoscape software to build a network control map of drugs-chemical components-targets-disease.Use R language software to screen common targets and find the four best The core targets of San for the treatment of acute gouty arthritis.The GO and KEGG analysis of the core targets were performed to clarify the core targets and mechanism of Si Miao decoction for the treatment of gout.Results:There are 64 effective chemical components in Si Miao decoction,197 genetic targets,600 disease targets,and 58 common targets.It is predicted that IL6,VEGFA,IL1B,JUN,PTGS2,and CCL2 may be treated by Si Miao decoction for gout The core target of arthritis.GO enrichment analysis identified 85 entries,of which biological processes mainly included the regulation of cytokine activity,protease activation,nucleic acid expression,etc.;KEGG pathway enrichment analysis identified 135 signaling pathways,of which signaling pathways involved the IL-17 signaling pathway,TNF signaling pathway,Th17 cell differentiation and other pathways.Conclusion:Si Miao decoction acts on acute gouty arthritis by regulating the occurrence of inflammation,and has significant anti-inflammatory and immune effects.The formula is rigorous and scientific,and it is worthy of clinical application.
文摘目的:观察凉血散瘀中药对糖尿病溃疡大鼠的干预作用,并从Wnt/β-catenin通路角度探讨其作用机制。方法:Wistar大鼠采用高脂高糖饲料联合小剂量链脲佐菌素(STZ)腹腔注射法制备糖尿病模型。分别取正常对照组与糖尿病组大鼠制作溃疡模型,并分为溃疡对照(CU)组、糖尿病溃疡(DU)组、四妙勇安汤组(DU-四妙组)、愈疡灵组(DU-愈疡灵组)、四妙勇安汤+愈疡灵组(DU-四妙+愈疡灵组),每组各15只。观察造模后第3、7、14 d各组创面愈合情况和织形态结构的变化的变化,采用ELISA法、RT-PCR法检测创面组织β-catenin、糖原合成激酶-3β(GSK-3β)蛋白及m RNA的表达水平。结果:DU组大鼠与CU组大鼠相比,第3、7、14 d的创面愈合率明显降低[(5.21±0.94)%vs(20.87±1.39)%,(31.70±1.21)%vs (49.99±1.58)%,(62.20±1.37)%vs (91.60±1.58)%, P <0.05];创面组织中β-catenin蛋白表达[(5.14±0.56) vs (9.04±0.65),(5.83±0.43) vs (10.08±0.35),(6.55±0.58) vs (12.77±0.30), P <0.05]及mRNA表达[(4.40±0.35) vs (6.92±0.16),(5.20±0.49) vs (7.12±0.48),(6.06±0.47) vs (9.24±0.55), P <0.05]均较低,而GSK-3β蛋白表达[(15.52±1.00) vs (7.04±0.53),(14.58±0.45) vs (6.05±0.23),(13.37±0.62) vs (4.49±0.37), P <0.05]及mRNA表达[(7.07±0.29) vs (3.55±0.32),(6.64±0.47) vs (3.00±0.28),(5.68±0.46) vs (1.98±0.19), P <0.05]则较高。与DU组大鼠相比,各治疗组大鼠各时间点的创面愈合率、创面组织中β-catenin蛋白及mRNA的表达水平均升高,而GSK-3β蛋白及mRNA的表达水平则降低,且治疗组中,以DU-四妙+愈疡灵组改善最明显。结论:具有凉血散瘀作用的四妙勇安汤及愈疡灵软膏均可通过干预Wnt/β-catenint通路的表达促进DU大鼠创面愈合,两者同用的效果优于单用其中一种。
文摘目的分析四妙勇安汤的研究热点、研究趋势和处方配伍规律。方法纳入中国知识资源总库(CNKI)、万方数据知识服务平台V2.0(万方数据)、维普中文期刊服务平台(VIP)、中国生物医学文献数据库(SinoMed)、Pubmed、Web of Science(WOS)自建库至2022年12月31日的四妙勇安汤相关文献为研究对象,应用CiteSpace软件进行可视化分析,统计具体频次;以2006~2022年的四妙勇安汤验案建立处方数据库,形成药物共现复杂网络,并进行分析。结果共纳入文献1086篇,医学院校及其附属医院是研究主力,血栓闭塞性脉管炎、糖尿病足、冠心病、痛风性关节炎、类风湿关节炎是四妙勇安汤的主治病种,四妙勇安汤及其加减方对相关疾病的治疗是研究重点,基础研究主要关注该方的抗动脉粥样硬化相关机制。该方常配伍黄芪、地黄、赤芍、苍术、黄柏等中药。结论四妙勇安汤是中医研究的热点方剂之一,其临床适应证的拓展和处方用药配伍有一定规律可循,其药理作用机制还需深入研究。
