海绵Sigmadocia sp.中的新神经酰胺的结构确定

从南海海绵Sigmadociasp.中分离得到两个新的神经酰胺 ,通过IR ,1DNMR ,2DNMR ,FABMS等波谱和化学方法 ,确定了它们的结构分别为N_(正_二十二碳酰基 )_2_氨基_正_二十烷_1,3,4,5_四羟醇(1)和N_(2′_羟基_正_二十二碳酰基 )_2_氨基_正_十八烷_1,3,4_三羟醇(2)。

Anti-dermatophytic activity of marine sponge,Sigmadocia carnosa(Dendy)on clinically isolated fungi

Objective:To screen the anti-fungal effects and find out the active metabolites from sponge, Sigmadocia carnosa(S.carnosa) against four dermatophytic fungi.Methods:The methanol,ethyl acetate and acetone extract of marine sponge,S.carnosa was examined against Trichaphyton mentagrophytes(T.mentagrophytes),Trichophyton rubrum(T.rubrum),Epidermophyton floccosum(E.floccosum) and Microsporum gypseum(M.gypseum) and qualitative analysed to find out the active molecules.Results:The methanol extract of sponge was expressed significant activity than ethyl acetate and acetone.The minimum inhibitory concentration(MIC) of methanol extract of sponge that resulted in complete growth inhibition of T.mentagrophytes,T.rubrum, E.floccosum and M.gypseum were found to 125,250,250 and 250 μg/mL respectively.But, 100%inhibition of fungal spore germination was observed in T.mentagrophytes at 500 μg/mL concentration followed by T.rubrum,E.floccosum and At.gypseum at 1000 μg/mL concentration. Other two extracts showed weak anti spore germination activity against the tested dermatophyte fungi.Methanol extracts showed presence of terpenoids,steroids,alkaloids,saponins and glycosides.Conclusion:Based on the literature,this is the first study which has conducted to inhibit the growth and spore germination of dermatophytic fungi with S.carnosa.Further research also needs to purify and characterize the secondary metabolites from the sponge,5.carnosa for the valuable source of novel substances for future drug discovery.

Anti-inflammatory,analgesic and antipyretic potentials of marine sponge Sigmadocia pumila

Objective:To study the pharmacological properties of Sigmadocia pumila(S.pumila),a marine sponge,through in-vivo analysis.Methods:The anti-inflammatory activity was determined by the carrageenan-induced rat paw edema method.The analgesic activity was analyzed by tail immersion method.Antipyretic activity was done by using Brewer’s yeast induced hyperpyrexia method.Results:The anti-inflammatory activity using methanol extracts in S.pumila at the concentrations of 100 mg/kg and 200 mg/kg,(p.o.)on rats showed significant decrease in the paw thickness at the 5th h of administration.It was denoted that the S.pumila exerted more analgesic activity.As for the antipyretic activity during the 2nd and 3rd h,the 3rd and 4th group of rats showed the reduction in temperature in S.pumila at 100 mg/kg and 200 mg/kg dosages.Conclusions:The present study concludes that the marine sponge S.pumila acts as a vital role in exhibiting pharmaceutical activities.It could be used to produce novel drugs.

莳萝曲网海绵化学成分的研究

从莳萝曲网海绵 ( Sigmadocia cymiformis Esper)的乙醇提取物中分离得到的 4个结晶性化合物 .通过波谱分析及化学降解等方法鉴定出它们的结构是麦角甾 -5 ,2 4 ( 2 8) -二烯 -3β-醇 ( 1 ) ,金色酰胺醇乙酸酯 ( 2 ) ,N-正三十烷酰 -十六碳鞘胺醇 ( 3)和胸腺嘧啶 ( 4 ) .其中 3为新化合物 ,命名为莳萝酰胺 ( cymiforamide) .金色酰胺醇乙酸酯 ( aurantiamide acetate) 2则是首次从海洋海绵中得到的一种变型二肽 .

莳萝曲网海绵中甾醇化合物的研究

应用色谱－质谱（GC－MS）方法分别鉴定了从广西北海涠洲岛水域和海南省陵水县水域采集的莳萝曲网海绵 Sigmadocia cymiformis的甾醇类化合物。涠洲岛水域的莳萝曲网海绵所含的甾醇类化合物主要是：胆甾－4，6－二烯－3β－醇（1），27－降麦角甾5，22－二烯－3β－醇（2），胆甾醇（３），（24S）－麦角甾－5，22－二烯－3β－醇（4），麦角甾－5－烯C－3β－醇（5），麦角甾－7，22－二烯－3β－醇（6），豆甾醇（７），β－谷甾醇（8），麦角甾－4，24（28）－二烯－3－酮（9），柳珊瑚甾醇（10）；而陵水县水域的莳萝曲网海绵所含甾醇类化合物主要是：（3），（4），（5），（6），（8），胆甾－5，22－二烯－3β－醇（11），胆甾－3，5－二烯－7－酮（12）。这是莳萝曲网海绵中甾醇类化合物组成的首次报道。