Traffic intersections are incredibly dangerous for drivers and pedestrians. Statistics from both Canada and the U.S. show a high number of fatalities and serious injuries related to crashes at intersections. In Canada...Traffic intersections are incredibly dangerous for drivers and pedestrians. Statistics from both Canada and the U.S. show a high number of fatalities and serious injuries related to crashes at intersections. In Canada, during 2019, the National Collision Database shows that 28% of traffic fatalities and 42% of serious injuries occurred at intersections. Likewise, the U.S. National Highway Traffic Administration (NHTSA) found that about 40% of the estimated 5,811,000 accidents in the U.S. during the year studied were intersection-related crashes. In fact, a major survey by the car insurance industry found that nearly 85% of drivers could not identify the correct action to take when approaching a yellow traffic light at an intersection. One major reason for these accidents is the “yellow light dilemma,” the ambiguous situation where a driver should stop or proceed forward when unexpectedly faced with a yellow light. This situation is even further exacerbated by the tendency of aggressive drivers to inappropriately speed up on the yellow just to get through the traffic light. A survey of Canadian drivers conducted by the Traffic Injury Research Foundation found that 9% of drivers admitted to speeding up to get through a traffic light. Another reason for these accidents is the increased danger of making a left-hand turn on yellow. According to the National Highway Traffic Safety Association (NHTSA), left turns occur in approximately 22.2% of collisions—as opposed to just 1.2% for right turns. Moreover, a study by CNN found left turns are three times as likely to kill pedestrians than right turns. The reason left turns are so much more likely to cause an accident is because they take a driver against traffic and in the path of oncoming cars. Additionally, most of these left turns occur at the driver’s discretion—as opposed to the distressingly brief left-hand arrow at busy intersections. Drive Safe Now proposes a workable solution for reducing the number of accidents occurring during a yellow light at intersections. We believe this fairly simple solution will save lives, prevent injuries, reduce damage to public and private property, and decrease insurance costs.展开更多
The expression patterns of OsPILll, one of six putative phytochrome-interacting factors, were analyzed in different organs of transgenic tobacco (Nicotiana tabacum). The expression of OsPIL 11 was organ-specific and...The expression patterns of OsPILll, one of six putative phytochrome-interacting factors, were analyzed in different organs of transgenic tobacco (Nicotiana tabacum). The expression of OsPIL 11 was organ-specific and was regulated by leaf development, abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). To further explore the role of OsPIL 11 in plant light signal transduction, a plant expression vector of OsPILll was constructed and introduced into tobacco. When grown under continuous red light, OsPILll-overexpressed transgenic tobacco exhibited shorter hypocotyls and larger cotyledons and leaves compared to wild-type seedlings. When grown under continuous far-red light, however, transgenic and wild-type seedlings showed similar phenotypes. These results indicate that OsPILll is involved in red light induced de-etiolation, but not in far-red light induced de-etiolation in transgenic tobacco, which lays the foundation for dissecting the function of OsPIL11 in phytochrome-mediated light signal transduction in rice.展开更多
提出一种基于旋转不变信号参数估计技术ESPRIT(Estimation of signal parameters via rotational invariance technique)、模式搜索算法PSA(Pattern search algorithm)与轻型梯度提升机LightGBM(Light gradient boosting machine)结合...提出一种基于旋转不变信号参数估计技术ESPRIT(Estimation of signal parameters via rotational invariance technique)、模式搜索算法PSA(Pattern search algorithm)与轻型梯度提升机LightGBM(Light gradient boosting machine)结合的感应电动机转子断条数目诊断新方法。模拟了转子断条故障下的瞬时无功功率信号并用其衡量ESPRIT-PSA的性能。结果表明:ESPRIT-PSA只需短时数据就能准确测量瞬时无功功率信号中的转子断条故障特征分量。随后,为解决现有的电机瞬时无功功率信号分析MIRPSA(Motor instantaneous reactive power signal analysis)类方法无法准确诊断转子断条数目的问题,引入LightGBM对转子断条故障进行多分类以准确诊断转子断条数目。最后针对一台异步电动机进行转子断条诊断实验,结果表明:该方法是有效的,并且因将瞬时无功功率作为分析信号而适用于电机低转差率的情况。展开更多
Arabidopsis seedlings undergo photomorphogenesis in the light and etiolation in the dark. HFR1, a bHLH transcription factor, is required for both phytochrome A (phyA)-mediated far-red and cryptochrome 1 (cry1)-mediate...Arabidopsis seedlings undergo photomorphogenesis in the light and etiolation in the dark. HFR1, a bHLH transcription factor, is required for both phytochrome A (phyA)-mediated far-red and cryptochrome 1 (cry1)-mediated blue light signaling. We report that HFR1 is a short-lived protein in darkness and is degraded through a 26S proteasome-dependent pathway. Light, irrespective of its quality, enhances HFR1 protein accumulation via promoting its stabilization. We demonstrate that HFR1 physically interacts with COP1 and that COP1 exhibits ubiquitin ligase activity toward HFR1 in vitro. In addition, we show that COP1 is required for degradation of HFR1 in vivo. Furthermore, plants overexpressing a C-terminal 161 amino acid fragment of HFR1 (CT161) display enhanced photomorphogenesis, suggesting an autonomous function of CT161 in promoting light signaling. This truncated HFR1 gene product is more stable than the full-length HFR1 protein in darkness, indicating that the COP1-interacting N-terminal portion of HFR1 is essential for COP1-mediated destabilization of HFR1. These results suggest that light enhances HFR1 protein accumulation by abrogating COP1-mediated degradation of HFR1, which is necessary and sufficient for promoting light signaling. Additionally, our results substantiate the E3 ligase activity of COP1 and its critical role in desensitizing light signaling.展开更多
We collected centric diatom Stephanopyxis palmeriana samples in coastal waters of Xiamen for charac- teristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual ...We collected centric diatom Stephanopyxis palmeriana samples in coastal waters of Xiamen for charac- teristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual repro- duction. The result showed that pre-illumination of 2-3h red light before darkness could induce sexualization of S. palmeriana, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment of S. palmeriana with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction of S. palmeriana is phytochrome B (phyB).展开更多
We experimentally study the controllable generation of a beating signal using stored light pulses based on electromagnetically induced transparency(EIT) in a solid medium. The beating signal relies on an asymmetric pr...We experimentally study the controllable generation of a beating signal using stored light pulses based on electromagnetically induced transparency(EIT) in a solid medium. The beating signal relies on an asymmetric procedure of light storage and retrieval. After storing the probe pulse into the spin coherence under the EIT condition, two-color control fields with opposite detunings instead of the initial control field are used to scatter the stored spin coherence. The controllable beating signal is generated due to alternative constructive and destructive interferences in the retrieved signal intensities. The beating of the two-color control fields is mapped into the beating of weak probe fields by using atomic spin coherence. This beating signal will be important in precise atomic spectroscopy and fast quantum limited measurements.展开更多
【目的】对城市交叉口采用的左转非机动车信号灯设施进行交通安全性量化评估。【方法】提出一种基于拓展碰撞时间(extended time to collision,ETTC)指标的左转非机动车信号灯安全效应评估方法。针对现有的碰撞时间(time to collision,T...【目的】对城市交叉口采用的左转非机动车信号灯设施进行交通安全性量化评估。【方法】提出一种基于拓展碰撞时间(extended time to collision,ETTC)指标的左转非机动车信号灯安全效应评估方法。针对现有的碰撞时间(time to collision,TTC)指标不适于评估交叉口左转非机动车冲突的问题,考虑非机动车车辆尺寸与加速度对交通冲突的影响,采用拓展碰撞时间指标,评估交叉口非机动车交通冲突。收集长沙市4个信号交叉口的视频大数据,利用视频软件Tracker提取车辆微观轨迹后,开展案例分析。【结果】左转非机动车信号灯在时间上明确了非机动车的通行权,其设置能显著降低非机动车冲突率,在平峰、高峰时段非机动车冲突率分别降低了40.11%、25.27%。在直行相位末期、左转相位即将启亮时,设置组的左转非机动车在待行区等待,冲突率降为0;而对比组近50%的非机动车违规左转,冲突严重。设置左转非机动车信号灯的改善效果随非机动车流量的增大呈先增加后降低趋势,而随机动车流量的增大呈逐步波动下降趋势。【结论】本研究揭示了非机动车左转信号灯的设置对减少交叉口交通冲突的影响,可为城市交叉口非机动车交通安全管控提供有益参考。展开更多
Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise ...Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise molecular mechanisms governing this process remain largely unknown.In this study,we revealed that high red to far-red(high R:FR)light significantly induces the synthesis of leaf starch and the expression of synthesis-related genes,whereas low R:FR light suppress these processes.Arabidopsis phytochrome B(phyB),the primary R and FR photoreceptor,was identified as a critical positive regulator in this process.Downstream of phyB,basic leucine zipper transcription factor ELONGATED HYPOCOTYL5(HY5)was found to enhance starch synthesis,whereas the basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORs(PIF3,PIF4,and PIF5)inhibit starch synthesis in Arabidopsis leaves.Notably,HY5 and PIFs directly compete for binding to a shared G-box cis-element in the promoter region of genes encoding starch synthases GBSS,SS3,and SS4,which leads to antagonistic regulation of their expression and,consequently,starch synthesis.Our findings highlight the vital role of phyB in enhancing starch synthesis by stabilizing HY5 and facilitating PIFs degradation under high R:FR light conditions.Conversely,under low R:FR light,PIFs predominantly inhibit starch synthesis.This study provides insight into the physiological and molecular functions of phyB and its downstream transcription factors HY5 and PIFs in starch synthesis regulation,shedding light on the regulatory mechanism by which plants synchronize dynamic light signals with metabolic cues to module starch synthesis.展开更多
Plants deploy versatile scaffold proteins to intricately modulate complex cell signaling.Among these,RACK1A(Receptors for Activated C Kinase 1A)stands out as a multifaceted scaffold protein functioning as a central in...Plants deploy versatile scaffold proteins to intricately modulate complex cell signaling.Among these,RACK1A(Receptors for Activated C Kinase 1A)stands out as a multifaceted scaffold protein functioning as a central integrative hub for diverse signaling pathways.However,the precise mechanisms by which RACK1A orchestrates signal transduction to optimize seedling development remain largely unclear.Here,we demonstrate that RACK1A facilitates hypocotyl elongation by functioning as a flexible platform that connects multiple key components of light signaling pathways.RACK1A interacts with PHYTOCHROME INTERACTING FACTOR(PIF)3,enhances PIF3 binding to the promoter of BBX11 and down-regulates its transcription.Furthermore,RACK1A associates with ELONGATED HYPOCOTYL 5(HY5)to repress HY5 biochemical activity toward target genes,ultimately contributing to hypocotyl elongation.In darkness,RACK1A is targeted by CONSTITUTIVELY PHOTOMORPHOGENIC(COP)1 upon phosphorylation and subjected to COP1-mediated degradation via the 26 S proteasome system.Our findings provide new insights into how plants utilize scaffold proteins to regulate hypocotyl elongation,ensuring proper skoto-and photo-morphogenic development.展开更多
文摘Traffic intersections are incredibly dangerous for drivers and pedestrians. Statistics from both Canada and the U.S. show a high number of fatalities and serious injuries related to crashes at intersections. In Canada, during 2019, the National Collision Database shows that 28% of traffic fatalities and 42% of serious injuries occurred at intersections. Likewise, the U.S. National Highway Traffic Administration (NHTSA) found that about 40% of the estimated 5,811,000 accidents in the U.S. during the year studied were intersection-related crashes. In fact, a major survey by the car insurance industry found that nearly 85% of drivers could not identify the correct action to take when approaching a yellow traffic light at an intersection. One major reason for these accidents is the “yellow light dilemma,” the ambiguous situation where a driver should stop or proceed forward when unexpectedly faced with a yellow light. This situation is even further exacerbated by the tendency of aggressive drivers to inappropriately speed up on the yellow just to get through the traffic light. A survey of Canadian drivers conducted by the Traffic Injury Research Foundation found that 9% of drivers admitted to speeding up to get through a traffic light. Another reason for these accidents is the increased danger of making a left-hand turn on yellow. According to the National Highway Traffic Safety Association (NHTSA), left turns occur in approximately 22.2% of collisions—as opposed to just 1.2% for right turns. Moreover, a study by CNN found left turns are three times as likely to kill pedestrians than right turns. The reason left turns are so much more likely to cause an accident is because they take a driver against traffic and in the path of oncoming cars. Additionally, most of these left turns occur at the driver’s discretion—as opposed to the distressingly brief left-hand arrow at busy intersections. Drive Safe Now proposes a workable solution for reducing the number of accidents occurring during a yellow light at intersections. We believe this fairly simple solution will save lives, prevent injuries, reduce damage to public and private property, and decrease insurance costs.
基金supported by grants from the Chinese National Natural Science Foundation (Grant No. 30971744)the Chinese Ministry of Agriculture(Grant No. 2009ZX08001-029B)the Shandong Natural Science Funds for Distinguished Young Scholar,China (Grant No. JQ200911)
文摘The expression patterns of OsPILll, one of six putative phytochrome-interacting factors, were analyzed in different organs of transgenic tobacco (Nicotiana tabacum). The expression of OsPIL 11 was organ-specific and was regulated by leaf development, abscisic acid (ABA), jasmonic acid (JA) and salicylic acid (SA). To further explore the role of OsPIL 11 in plant light signal transduction, a plant expression vector of OsPILll was constructed and introduced into tobacco. When grown under continuous red light, OsPILll-overexpressed transgenic tobacco exhibited shorter hypocotyls and larger cotyledons and leaves compared to wild-type seedlings. When grown under continuous far-red light, however, transgenic and wild-type seedlings showed similar phenotypes. These results indicate that OsPILll is involved in red light induced de-etiolation, but not in far-red light induced de-etiolation in transgenic tobacco, which lays the foundation for dissecting the function of OsPIL11 in phytochrome-mediated light signal transduction in rice.
文摘提出一种基于旋转不变信号参数估计技术ESPRIT(Estimation of signal parameters via rotational invariance technique)、模式搜索算法PSA(Pattern search algorithm)与轻型梯度提升机LightGBM(Light gradient boosting machine)结合的感应电动机转子断条数目诊断新方法。模拟了转子断条故障下的瞬时无功功率信号并用其衡量ESPRIT-PSA的性能。结果表明:ESPRIT-PSA只需短时数据就能准确测量瞬时无功功率信号中的转子断条故障特征分量。随后,为解决现有的电机瞬时无功功率信号分析MIRPSA(Motor instantaneous reactive power signal analysis)类方法无法准确诊断转子断条数目的问题,引入LightGBM对转子断条故障进行多分类以准确诊断转子断条数目。最后针对一台异步电动机进行转子断条诊断实验,结果表明:该方法是有效的,并且因将瞬时无功功率作为分析信号而适用于电机低转差率的情况。
文摘Arabidopsis seedlings undergo photomorphogenesis in the light and etiolation in the dark. HFR1, a bHLH transcription factor, is required for both phytochrome A (phyA)-mediated far-red and cryptochrome 1 (cry1)-mediated blue light signaling. We report that HFR1 is a short-lived protein in darkness and is degraded through a 26S proteasome-dependent pathway. Light, irrespective of its quality, enhances HFR1 protein accumulation via promoting its stabilization. We demonstrate that HFR1 physically interacts with COP1 and that COP1 exhibits ubiquitin ligase activity toward HFR1 in vitro. In addition, we show that COP1 is required for degradation of HFR1 in vivo. Furthermore, plants overexpressing a C-terminal 161 amino acid fragment of HFR1 (CT161) display enhanced photomorphogenesis, suggesting an autonomous function of CT161 in promoting light signaling. This truncated HFR1 gene product is more stable than the full-length HFR1 protein in darkness, indicating that the COP1-interacting N-terminal portion of HFR1 is essential for COP1-mediated destabilization of HFR1. These results suggest that light enhances HFR1 protein accumulation by abrogating COP1-mediated degradation of HFR1, which is necessary and sufficient for promoting light signaling. Additionally, our results substantiate the E3 ligase activity of COP1 and its critical role in desensitizing light signaling.
基金Supported by National Natural Science Foundation of China (No. 39670076)
文摘We collected centric diatom Stephanopyxis palmeriana samples in coastal waters of Xiamen for charac- teristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual repro- duction. The result showed that pre-illumination of 2-3h red light before darkness could induce sexualization of S. palmeriana, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment of S. palmeriana with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction of S. palmeriana is phytochrome B (phyB).
基金Project supported by the National Basic Research Program of China (Grant No. 201 ICB921603), the National Natural Science Foundation of China (Grant Nos. 11374126, 10904048, 11074097, 11004079, 11004080, and 11247201), the China Postdoctoral Science Foundation (Grant Nos. 2011M500924 and 2013T60317). and the National Fund for Fosterin~ Talents of Basic Science (Grant No. J I 103202).
文摘We experimentally study the controllable generation of a beating signal using stored light pulses based on electromagnetically induced transparency(EIT) in a solid medium. The beating signal relies on an asymmetric procedure of light storage and retrieval. After storing the probe pulse into the spin coherence under the EIT condition, two-color control fields with opposite detunings instead of the initial control field are used to scatter the stored spin coherence. The controllable beating signal is generated due to alternative constructive and destructive interferences in the retrieved signal intensities. The beating of the two-color control fields is mapped into the beating of weak probe fields by using atomic spin coherence. This beating signal will be important in precise atomic spectroscopy and fast quantum limited measurements.
文摘【目的】对城市交叉口采用的左转非机动车信号灯设施进行交通安全性量化评估。【方法】提出一种基于拓展碰撞时间(extended time to collision,ETTC)指标的左转非机动车信号灯安全效应评估方法。针对现有的碰撞时间(time to collision,TTC)指标不适于评估交叉口左转非机动车冲突的问题,考虑非机动车车辆尺寸与加速度对交通冲突的影响,采用拓展碰撞时间指标,评估交叉口非机动车交通冲突。收集长沙市4个信号交叉口的视频大数据,利用视频软件Tracker提取车辆微观轨迹后,开展案例分析。【结果】左转非机动车信号灯在时间上明确了非机动车的通行权,其设置能显著降低非机动车冲突率,在平峰、高峰时段非机动车冲突率分别降低了40.11%、25.27%。在直行相位末期、左转相位即将启亮时,设置组的左转非机动车在待行区等待,冲突率降为0;而对比组近50%的非机动车违规左转,冲突严重。设置左转非机动车信号灯的改善效果随非机动车流量的增大呈先增加后降低趋势,而随机动车流量的增大呈逐步波动下降趋势。【结论】本研究揭示了非机动车左转信号灯的设置对减少交叉口交通冲突的影响,可为城市交叉口非机动车交通安全管控提供有益参考。
基金supported by the National Natural Science Foundation of China(32270263)the Shandong Provincial Natural Science Foundation(ZR2022QC095,ZR2020MC023,ZR2022MC019).
文摘Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise molecular mechanisms governing this process remain largely unknown.In this study,we revealed that high red to far-red(high R:FR)light significantly induces the synthesis of leaf starch and the expression of synthesis-related genes,whereas low R:FR light suppress these processes.Arabidopsis phytochrome B(phyB),the primary R and FR photoreceptor,was identified as a critical positive regulator in this process.Downstream of phyB,basic leucine zipper transcription factor ELONGATED HYPOCOTYL5(HY5)was found to enhance starch synthesis,whereas the basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORs(PIF3,PIF4,and PIF5)inhibit starch synthesis in Arabidopsis leaves.Notably,HY5 and PIFs directly compete for binding to a shared G-box cis-element in the promoter region of genes encoding starch synthases GBSS,SS3,and SS4,which leads to antagonistic regulation of their expression and,consequently,starch synthesis.Our findings highlight the vital role of phyB in enhancing starch synthesis by stabilizing HY5 and facilitating PIFs degradation under high R:FR light conditions.Conversely,under low R:FR light,PIFs predominantly inhibit starch synthesis.This study provides insight into the physiological and molecular functions of phyB and its downstream transcription factors HY5 and PIFs in starch synthesis regulation,shedding light on the regulatory mechanism by which plants synchronize dynamic light signals with metabolic cues to module starch synthesis.
基金supported by grants from National Natural Science Foundation of China(32070292 to J.L.)Shenzhen Science and Technology Program(KQTD20190929173906742 to J.L.)+1 种基金The Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes(2019KSYS006 to J.L.)Scientific Research Funding for postdoctoral researchers staying at Shenzhen(K19227561,K21227504 to W.Z.).
文摘Plants deploy versatile scaffold proteins to intricately modulate complex cell signaling.Among these,RACK1A(Receptors for Activated C Kinase 1A)stands out as a multifaceted scaffold protein functioning as a central integrative hub for diverse signaling pathways.However,the precise mechanisms by which RACK1A orchestrates signal transduction to optimize seedling development remain largely unclear.Here,we demonstrate that RACK1A facilitates hypocotyl elongation by functioning as a flexible platform that connects multiple key components of light signaling pathways.RACK1A interacts with PHYTOCHROME INTERACTING FACTOR(PIF)3,enhances PIF3 binding to the promoter of BBX11 and down-regulates its transcription.Furthermore,RACK1A associates with ELONGATED HYPOCOTYL 5(HY5)to repress HY5 biochemical activity toward target genes,ultimately contributing to hypocotyl elongation.In darkness,RACK1A is targeted by CONSTITUTIVELY PHOTOMORPHOGENIC(COP)1 upon phosphorylation and subjected to COP1-mediated degradation via the 26 S proteasome system.Our findings provide new insights into how plants utilize scaffold proteins to regulate hypocotyl elongation,ensuring proper skoto-and photo-morphogenic development.