Hypothalamic-Pituitary-Gonadal(HPG)Axis and Transcriptional Regulatory Elements Regulate piwil2 Gene Expression During Gametogenesis and Gonadal Development in Japanese Flounder(Paralichthys olivaceus)

The P-element induced wimpy testis(Piwi)proteins,which are associated with PIWI-interacting RNAs(piRNAs),play important roles in meiosis,germ cell division,and germline maintenance.In this study,we identified and characterized the Paralichthys olivaceus piwil2 gene,a constituent factor of the piRNA pathways involved in the biogenesis of reproductive development.The biological analysis indicated that piwil2,which contains PAZ and PIWI domains,was highly conserved between teleosts and tetrapods.The piwil2 distribution profile in different tissues confirmed a sexually dimorphic expression pattern,with a higher expression level in testis.In situ hybridization demonstrated that piwil2 was expressed in the oogonia and oocytes of the ovaries as well as in the Sertoli cells and spermatocytes of the testes.Gene piwil2 showed a maternally inherited expression pattern during embryonic development,and was highly expressed during the early embryonic development.Different luciferase reporters were constructed to determine the transcriptional regulatory mechanisms of piwil2.The piwil2 core promoter region was located at−360 bp to−60 bp.Furthermore,some representative sex hormones,including human chorionic gonadotropin,17α-methyltestosterone,and estradiol-17βhad distinct regulatory effects on piwil2.In a summery,these results indicate that piwil2,regulated by sex hormones and transcriptional elements,has vital functions in the reproductive cycle and gonadal development.

Features,Mechanisms and Applies of Post-transcriptional Gene Silencing in Transgenic Plants

Since transgene silencing was found in transgenic plants,many scholars have studied it extensively and considered that it has three functional mechanisms:post dependent gene silencing,transcriptional gene silencing,post transcriptional gene silencing.At the moment,people have mainly focused on the study of post transcriptional gene silencing and found its features:extensivity,conduction and peculiarity,also put forward some hypothesis for its mechanisms,for example,RNA threshold model,aberrant RNA model,inter or intra molecular base pairing model and so on.Furthermore,post transcriptional gene silencing is being applied in gene engineering of plants.Recently the people have found that post transcriptional gene silencing has bearing on capacity plants resisting virus.Many researchers have studied post transcriptional gene silencing,but there are some questions which need be solved in the future.This article summarizes progresses in features,mechanisms,applies of post transcriptional gene silencing about transgenic plants.

Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus

While human immunodeficiency virus 1(HIV-1) infectionis controlled through continuous, life-long use of a combination of drugs targeting different steps of the virus cycle, HIV-1 is never completely eradicated from the body. Despite decades of research there is still no effective vaccine to prevent HIV-1 infection. Therefore, the possibility of an RNA interference(RNAi)-based cure has become an increasingly explored approach. Endogenous gene expression is controlled at both, transcriptional and post-transcriptional levels by noncoding RNAs, which act through diverse molecular mechanisms including RNAi. RNAi has the potential to control the turning on/off of specific genes through transcriptional gene silencing(TGS), as well as finetuning their expression through post-transcriptional gene silencing(PTGS). In this review we will describe in detail the canonical RNAi pathways for PTGS and TGS, the relationship of TGS with other silencing mechanisms and will discuss a variety of approaches developed to suppress HIV-1 via manipulation of RNAi. We will briefly compare RNAi strategies against other approaches developed to target the virus, highlighting their potential to overcome the major obstacle to finding a cure, which is the specific targeting of the HIV-1 reservoir within latently infected cells.

Studies on Trans-Generational Transcriptional Silencing of <i>cry</i>1<i>Ac</i>Gene in Tobacco Transgenics

Developing transgenics that express high levels of Cry1Ac protein, and at the same time, are phenotypically normal, has not been an easy task to achieve. It has been routinely observed that most of the transgenic plants that survive, show no or extremely low levels of Cry1Ac protein. However, all of these plants do express the selectable marker, nptII gene. In the present study, we record an interesting observation of how one of the genes (cry1Ac) on a single T-DNA fragment is selectively silenced, keeping the expression of the other gene (nptII) intact. Further, this silenced state is inherited.

Preliminary identification and analysis of point mutations corre lated with response to interferon-α in hepatitis B virus post-transcriptional regulatory elements

Background It is still unclear whether viral genetic variability influences response to interferon(IFN) α treatment Recent reports suggest that IFN α effects may be associated with hepatitis B virus(HBV) post transcriptional regulation This study was designed to explore the heterogeneity of HBV post transcriptional regulatory elements (HPRE) and the relationship between the diversity of HPRE and the response to IFN α treatment Methods The HPRE sequences from 31 Chinese patients infected with HBV were determined by directly sequencing of polymerase chain reaction (PCR) product, and comparing them to those from Caucasian patients Subsequently, eukaryotic expression vectors containing HPRE at various points were constructed and transfected into HepG2 cells, which were then exposed to recombinant human cytokines Results The T to C point mutation at nt 1504 and the C to T (G) at nt 1508 in HPRE were found in 21 and 19 patients with chronic hepatitis B, respectively; the C to T point mutation at nt 1509 was found in 17 patients These point mutations did not exist in the HPRE of the Caucasian patients The activity of the CAT gene obviously increased in the case of T to C point mutation at nt 1504, but did not change in the case of the C to T (G) mutations at nt 1508 and 1509 The activity of the CAT gene at these point mutations of HPRE could be inhibited by IFN α/γ and tumor necrosis factor (TNF) α except for the point mutations at nt 1508 of HPRE which may escape the suppression role of IFN α on HPRE Conclusions There are point mutations between the HPRE of Chinese and Caucasian HBV patients, which might be correlated with response to IFN α The variation of HPRE might affect the function of HPRE and influence the regulative function of IFN α other than that of IFN γ or TNF α on HPRE

Dual activities of a silencing information regulator complex in yeast transcriptional regulation and DNA-damage response

The Saccharomyces cerevisiae silencing information regulator(SIR)complex contains up to four proteins,namely Sir1,Sir2,Sir3,and Sir4.While Sir2 encodes a NAD-dependent histone deacetylase,other SIR proteins mainly function as structural and scaffold components through physical interaction with various proteins.The SIR complex displays different conformation and composition,including Sir2 homotrimer,Sir1-4 heterotetramer,Sir2-4 heterotrimer,and their derivatives,which recycle and relocate to different chromosomal regions.Major activities of the SIR complex are transcriptional silencing through chromosomal remodeling and modulation of DNA double-strand-break repair pathways.These activities allow the SIR complex to be involved in mating-type maintenance and switching,telomere and subtelomere gene silencing,promotion of nonhomologous end joining,and inhibition of homologous recombination,as well as control of cell aging.This review explores the potential link between epigenetic regulation and DNA damage response conferred by the SIR complex under various conditions aiming at understanding its roles in balancing cell survival and genomic stability in response to internal and environmental stresses.As core activities of the SIR complex are highly conserved in eukaryotes from yeast to humans,knowledge obtained in the yeast may apply to mammalian Sirtuin homologs and related diseases.

Repressor elements provide insights into tissue development and phenotypes in pigs

Repressor elements significantly influence economically relevant phenotypes in pigs;however,their precise roles and characteristics are inadequately understood.In the present study,we employed H3K27me3 profiling,assay for transposase-accessible chromatin with highthroughput sequencing(ATAC-seq),and RNA sequencing(RNA-seq)data across six tissues derived from three embryonic layers to identify and map 2034 super repressor elements(SREs) and 22223 typical repressor elements(TREs) in the pig genome.Notably,many repressor elements were conserved across mesodermal and ectodermal tissues.SREs exhibited tight regulation of their target genes,affecting a limited number of genes within a specific genomic region with pronounced effects,while TREs exerted broader but weaker regulation over a wider range of target genes.Furthermore,in neuronal tissues,genes regulated by repressor elements started to be repressed during the differentiation of stem cells into progenitor cells.Notably,analysis showed that many repressor elements exhibited cooperative and additive effects on the modulation of KLF4 expression.This research provides the first comprehensive map of pig repressor elements,serving as an essential reference for future studies on repressor elements.

Pig H3K4me3,H3K27ac,and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

Regulatory sequences and transposable elements(TEs)account for a large proportion of the genomic sequences of species;however,their roles in gene transcription,especially tissue-specific expression,remain largely unknown.Pigs serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated populations.Here,we conducted an integrated analysis using H3K27ac ChIP-seq,H3K4me3 ChIP-seq,and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult pigs.We aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental stages.Based on correlation analysis between mRNA expression and H3K27ac and H3K4me3 peak activity,results indicated that H3K27ac exhibited stronger associations with gene expression than H3K4me3.Furthermore,1.45%of TEs overlapped with either the H3K27ac or H3K4me3 peaks,with the majority displaying tissue-specific activity.Notably,a TE subfamily(LTR4C_SS),containing binding motifs for SIX1 and SIX4,showed specific enrichment in the H3K27ac peaks of the adult and fetal ovaries.RNA-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes,including 4688 TE-containing transcripts with distinct development stage-specific and tissue-specific expression.Of note,1967 TE-containing transcripts were enriched in the testes.We identified a long terminal repeat(LTR),MLT1F1,acting as a testis-specific alternative promoter in SRPK2(a cell cycle-related protein kinase)in our pig dataset.This element was also conserved in humans and mice,suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary patterns.Collectively,our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions,particularly in the reproductive organs.

The Splicing Factor PRP31 Is Involved in Transcriptional Gene Silencing and Stress Response in Arabidopsis

Although DNA methylation is known to play an important role in the silencing of transposable elements （TEs） and introduced transgenes, the mechanisms that generate DNA methylation-independent transcrip- tional silencing are poorly understood. Previous studies suggest that RNA-directed DNA methylation （RdDM） is required for the silencing of the RD29A-LUC transgene in the Arabidopsis rosl mutant back- ground with defective DNA demethylase. Loss of function of ARGONAUTE 4 （AGO4） gene, which encodes a core RdDM component, partially released the silencing of RD29A-LUC in the rosl/ago4 double mutant plants. A forward genetic screen was performed to identify the mutants with elevated RD29A-LUC trans- gene expression in the rosl/ago4 mutant background. We identified a mutation in the homologous gene of PRP31, which encodes a conserved pre-mRNA splicing factor that regulates the formation of the U4/ U6.U5 snRNP complex in fungi and animals. We previously demonstrated that the splicing factors ZOP1 and STA1 contribute to transcriptional gene silencing. Here, we reveal that Arabidopsis PRP31 associates with ZOP1, STA1, and several other splicing-related proteins, suggesting that these splicing factors are both physically and functionally connected. We show that Arabidopsis PRP31 participates in transcrip- tional gene silencing. Moreover, we report that PRP31, STA1, and ZOP1 are required for development and stress response. Under cold stress, PRP31 is not only necessary for pre-mRNA splicing but also for regulation of cold-responsive gene expression. Our results suggest that the splicing machinery has multiple functions including pre-mRNA splicing, gene regulation, transcriptional gene silencing, and stress response.

Sumoylation of SUVR2 contributes to its role in transcriptional gene silencing

The SU(VAR)-3-9-related protein family member SUVR2 has been previously identified to be involved in transcriptional gene silencing both in RNA-dependent and-independent pathways. It interacts with the chromatin-remodeling proteins CHR19,CHR27, and CHR28(CHR19/27/28), which are also involved in transcriptional gene silencing. Here our study demonstrated that SUVR2 is almost fully mono-sumoylated in vivo. We successfully identified the exact SUVR2 sumoylation site by combining in vitro mass spectrometric analysis and in vivo immunoblotting confirmation. The luminescence imaging assay and quantitative RT-PCR results demonstrated that SUVR2 sumoylation is involved in transcriptional gene silencing. Furthermore, we found that SUVR2 sumoylation is required for the interaction of SUVR2 with CHR19/27/28, which is consistent with the fact that SUMO proteins are necessary for transcriptional gene silencing. These results suggest that SUVR2 sumoylation contributes to transcriptional gene silencing by facilitating the interaction of SUVR2 with the chromatin-remodeling proteins CHR19/27/28.

Transcriptional activation of Nlp by estrogen-ERa in breast cancer

Estrogen Receptor-a （ERa） is the key transcription factor that regulates cell proliferation and homeosta- sis. In this pathway, estrogen plays an important role in genomic instability and cell cycle regulation pro- cesses and the mechanisms of its action are multifaceted. In this study, we showed that estrogen regulates genomic instability through promoting the expression of Nip, a BRCAl-associated centrosomal protein which is involved in microtubule nucleation, spindle formation, chromosomal missegregation and abnormal cytokinesis. We demonstrated that the expression of Nip is strongly associated with ERa and FOXAI level in clinical breast cancer samples with poor clinical outcomes to breast cancer patients. Addition of estrogen in the ER-positive breast cancer cells resulted in elevation of NLP mRNA. Significantly, we identified that estrogen-ERa is capable of regulating Nlp expression through specifically binding ERa to the proximal region and the Estrogen Responsive Elements （ERE） enhancer in the distal region of NLP gene. Reporter assays demonstrated that estrogen directly activated Nlp promoter. ChIP assay results showed that E2-ERa directly bound to the EREs of Nip. Therefore, overexpression of Nip in breast cancer exhibits a hormone-dependent pattern, and estrogen participates in the regulation of genome instability and cell cycle in breast cancer cells partially through transcriptional activation of NLP gene. Overexpression of Nlp enhances the malignant progression of ERa-positive breast cancer cells in vitro, whereas knockdown of Nip suppresses this biological effects in ERa-positive breast cancer ceils. ERa/NIp axis may serve as a promising target against breast cancer.

Transcriptional Regulatory Networks Activated by PI3K and ERK Transduced Growth Signals in Human Glioblastoma Cells

Determining how cells regulate their transcriptional response toextracellular signals is key to the understanding of complex eukaryotic systems. This study wasinitiated with the goals of furthering the study of mammalian transcriptional regulation andanalyzing the relative benefits of related computational methodologies. One dataset available forsuch an analysis involved gene expression profiling of the early growth factor response to plateletderived growth factor (PDGF) in a human glioblastoma cell line; this study differentiated geneswhose expression was regulated by signaling through the phosphoinositide-3-kinase (PI3K) versus theextracellular-signal regulated kinase (ERK) pathways. We have compared the inferred transcriptionfactors from this previous study with additional predictions of regulatory transcription factorsusing two alternative promoter sequence analysis techniques. This comparative analysis, in which thealgorithms predict overlapping, although not identical, sets of factors, argues for meticulousbenchmarking of promoter sequence analysis methods to determine the positive and negative attributesthat contribute to their varying results. Finally, we inferred transcriptional regulatory networksderiving from various signaling pathways using the CARRIE program suite. These networks not onlyincluded previously described transcriptional features of the response to growth signals, but alsopredicted new regulatory features for the propagation and modulation of the growth signal.

MicroRNA Primary Transcripts and Promoter Elements Analysis in Soybean (Glycine max L. Merril.)

The importance of microRNA （miRNA） at the post-transcriptional regulation level has recently been recognized in both animals and plants. In recent years, many studies focused on miRNA target identification and functional analysis. However, little is known about the transcription and regulation of miRNAs themselves. In this study, the transcription start sites （TSSs） for 11 miRNA primary transcripts of soybean from 11 miRNA loci （of 50 loci tested） were cloned by a 5＂ rapid amplification of cDNA ends （5＂ RACE） procedure using total RNA from 30-d-old seedlings. The features consistent with a RNA polymerase II mechanism of transcription were found among these miRNA loci. A position weight matrix algorithm was used to identify conserved motifs in miRNA core promoter regions. A canonical TATA box motif was identified upstream of the major start site at 8 （76%） of the mapped miRNA loci. Several cis-acting elements were predicted in the 2 kb 5＂ to the TSSs. Potential spatial and temporal expression patterns of the miRNAs were found. The target genes for these miRNAs were also predicted and further elucidated for the potential function of the miRNAs. This research provides a molecular basis to explore regulatory mechanisms of miRNA expression, and a way to understand miRNA-mediated regulatory pathways and networks in soybean.

Application of dual reciprocity boundary element method to predict acoustic attenuation characteristics of marine engine exhaust silencers

In marine engine exhaust silencing systems, the presence of exhaust gas flow influences the sound propagation inside the systems and the acoustic attenuation performance of silencers. In order to investigate the effects of three-dimensional gas flow and acoustic damping on the acoustic attenuation characteristics of marine engine exhaust silencers, a dual reciprocity boundary element method (DRBEM) was developed. The acoustic governing equation in three-dimensional potential flow was derived first, and then the DRBEM numerical procedure is given. Compared to the conventional boundary element method (CBEM), the DRBEM considers the second order terms of flow Mach number in the acoustic governing equation, so it is suitable for the cases with higher Mach number subsonic flow. For complex exhaust silencers, it is difficult to apply the single-domain boundary element method, so a substructure approach based on the dual reciprocity boundary element method is presented. The experiments for measuring transmission loss of silencers are conducted, and the experimental setup and measurements are explained. The transmission loss of a single expansion chamber silencer with extended inlet and outlet were predicted by DRBEM and compared with the measurements. The good agreements between predictions and measurements are observed, which demonstrated that the derived acoustic governing equation and the DRBEM numerical procedure in the present study are correct.

An <i>in silico</i>Analysis of Upstream Regulatory Modules (URMs) of Tapetum Specific Genes to Identify Regulatory <i>cis</i>-Elements and Transcription Factors

The present work presents an iin silicoi analysis of Upstream Regulatory Modules (URMs) of genes expressed in tapetum specific manner in dicotyledon and monocotyledon plants. In the current analysis, we identified several motifs conserved in these URMs of which ten were observed to be part of known icisi-elements using tools and databases like MEME, PLACE, MAST and TFSEARCH. We also identified that binding sites for two transcription factors, DOF and WRKY71 were found to be present in majority of the URMs.

冠心病患者血清环磷酸腺苷反应元件结合蛋白调节转录辅激活因子3及氧化应激指标与颈动脉粥样硬化的相关性

目的探讨冠心病患者血清环磷酸腺苷反应元件结合蛋白调节转录辅激活因子3(CRTC3)及氧化应激指标与颈动脉粥样硬化的相关性。方法选取2021年6月—2023年6月本院收治的154例冠心病患者为研究组,根据颈动脉粥样硬化程度分为轻度硬化组、中度硬化组和重度硬化组;另选取154例同期健康体检者为对照组。采用Pearson法分析血清CRTC3及氧化应激指标与颈动脉粥样硬化指标的相关性。结果研究组血清CRTC3、丙二醛(MDA)、颈动脉斑块面积和中层内膜厚度(IMT)高于或大于对照组,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平低于对照组,差异有统计学意义(P<0.05)。轻度硬化组、中度硬化组和重度硬化组血清CRTC3、MDA、颈动脉斑块面积和IMT依次升高或增大,SOD、GSH-Px水平依次降低,差异有统计学意义(P<0.05)。Pearson相关性分析显示,血清CRTC3、MDA水平与颈动脉斑块面积、IMT呈正相关(P<0.05),SOD、GSH-Px与颈动脉斑块面积、IMT呈负相关(P<0.05)。受试者工作特征(ROC)曲线显示,CRTC3、SOD、MDA和GSH-Px联合诊断重度颈动脉粥样硬化的曲线下面积(AUC)为0.990(95%CI:0.982~0.998),灵敏度为96.27%,特异度为76.28%。4项指标联合诊断的价值高于各指标单独诊断,差异有统计学意义(Z_(联合-CRTC3)=2.723,Z_(联合-SOD)=2.698,Z_(联合-MDA)=2.673,Z_(联合-GSH-Px)=2.803,P均<0.05)。结论冠心病患者血清CRTC3、MDA水平显著升高,SOD、GSH-Px水平显著降低;血清CRTC3、氧化应激水平均与颈动脉粥样硬化密切相关。

热激转录因子HSF调控植物非生物胁迫响应的作用机制

热激转录因子(Heat shock transcription factors,HSF)是植物抵抗热胁迫最重要的转录因子家族之一,广泛存在于真核生物。HSF拥有高度保守的DNA结合域,可参与复杂的胁迫信号转导和响应网络。HSF作为信号转导链的末端组分,介导非生物逆境胁迫下靶标基因的表达。在逆境胁迫响应中,HSF受上游蛋白激酶介导磷酸化和泛素化或通过ABA信号通路结合热激元件,调控热激蛋白等下游基因的表达,从而提高植物的抗逆性。本文综述HSF的发现、结构、分类、调控机制及其在植物响应非生物逆境胁迫(极端温度、干旱、高盐碱和重金属等)中的作用机制,并对未来研究方向加以展望,以期为农作物和牧草抗逆性遗传改良提供理论依据和基因资源。

换锦花LsMYB7基因克隆与功能研究

【目的】研究转录因子LsMYB7基因对换锦花Lycoris sprengeri花色苷积累的调控作用。【方法】采用实时荧光定量PCR(RT-qPCR)方法从换锦花花瓣中克隆获得花色苷形成相关R2R3-MYB转录因子LsMYB7基因,并进行生物信息学分析,再通过病毒介导的基因沉默(VIGS)技术研究LsMYB7基因对花色苷积累的调控作用。【结果】克隆到1条长951 bp的LsMYB7基因cDNA序列,开放阅读框(ORF)为825 bp,编码274个氨基酸,LsMYB7蛋白含有2个R2和R3结构域,属R2R3-MYB转录因子家族;系统进化分析表明LsMYB7与拟南芥Arabidopsis thaliana S22亚族基因聚为一类;LsMYB7亚细胞定位于细胞核,在不同花发育阶段和不同花色无性系中,LsMYB7基因表达与花色苷合成相关基因的表达趋势一致,主要在败花期和花色苷含量较高的H1无性系中表达;LsMYB7基因沉默后,换锦花花瓣明显变短,颜色变深,且LsCHS、LsF3'H、LsANS、LsUFGT1和LsUFGT2等花色苷形成相关基因的表达显著下调。【结论】LsMYB7属R2R3-MYB转录因子家族S22亚族,通过正向调控LsCHS、LsF3'H、LsANS、LsUFGT1和LsUFGT2花色苷生物合成相关基因的表达促进花色苷积累。

单元并排式阻性消声器消声量计算方法

采用Belov公式计算单元并排式阻性消声器消声量误差较大,为提高计算精度,按以下步骤构建单元并排式消声器消声量计算模型。(1)将消声器划分为角单元、边单元和内部单元等3种基本单元,采用Belov公式计算各基本单元传递损失(Transmission Loss,TL);(2)假设消声器入口端声能均匀分布,根据各基本单元入口端声功率和传递损失计算公式确定其出口端声功率;(3)根据消声器入口端和出口端总声功率得到消声量理论值TLt;(4)将11425 Pa·s/m^(2)作为流阻率基准值,通过有限元仿真得到采用该流阻率多孔吸声材料的消声器消声量仿真值TLs,得到仿真值和理论值的比值K_(1)(即TLs/TLt);(5)通过仿真进一步确定多孔吸声材料流阻率和基准流阻率不同情况下消声器消声量的比值K_(2),拟合获得K_(2)与流阻率σ的关系函数K_(2)(σ);(6)建立单元并排式阻性消声器消声量计算模型TL=TLt·K_(1)·K_(2)(σ)。实测结果表明,根据该模型计算得到的消声器各倍频带传递损失值与实测值绝对误差均小于2 d B,相对误差均小于10%。模型适用于计算采用不同多孔吸声材料、具有不同结构尺寸的单元并排式阻性消声器的消声量。

非生物胁迫对毛竹转座子衍生TUCP转录活性的影响

【目的】转座子(TE)是真核细胞基因组的重要组成部分,在毛竹Phyllostachys edulis基因组超过63%时,易受胁迫诱导激活。分析非生物胁迫下,来源于转座子的不确定编码潜力转录本(TUCP)的表达模式,为转座子参与毛竹抗逆分子机制提供参考。【方法】采用生物信息学技术和手段,在低温、高温、高盐、紫外照射等4种胁迫处理下,研究毛竹TE-TUCPs及转座子邻近基因的转录特性和转录模式。通过实时荧光定量PCR(RT-qPCR)验证转录组来源的TETUCPs差异表达数据的可靠性。【结果】在毛竹4个胁迫处理转录本中,共鉴定出57627个TE-TUCPs。TE-TUCPs应对不同非生物胁迫表现出特异性表达模式。高温、高盐、紫外照射处理可以促进具有转录活性的TE-TUCPs附近基因差异表达,但是低温会抑制具有转录活性的TE-TUCPs附近基因差异表达。【结论】TE-TUCPs主要来源于Ty1/Copia和Ty3/Gypsy超家族。基因的表达潜能与近距离的TE-TUCPs表达潜能互相抑制。TE-TUCPs转录情况会受到非生物胁迫作用来调控附近基因的表达以适应胁迫影响。