Silk Fibroin-modified Poly(butadiene) urethane Films and Their Effects on Fibroblast Viability

Surface-modified poly(butadiene)urethane (PBTU) films with silk fibroin (SF) were prepared by simple chemical method under the normal temperature. The physical properties and biological behaviour of the SF-modified PBTU film were evaluated. The results showed that the SF-modified PBTU films kept the tenacity and pliability very well, and could overcome rigid and brittle weaks of silk fibroin films. The morphology of SF in the PBTU film was dendritic aggregations, and the water-contact angle measurement indicated that the surface hydrophilicity of modified films was apparently enhanced. The biocompatibility of PBTU films was improved due to the change of surface components. The degree of platelet adhesion and the cell viability of rat embryo dermal fibroblasts seeded on PBTU films, SF films, and SF-modified PBTU films were measured by counting platelets before and after they contacted the films and MTT assay, respectively. The results indicated that platelet adhesion resistance and cell viability on the modified film were greatly superior to those on the PBTU film and the compound interface had good stability in the air.

Effects of Nano-patterning Modification on the Cell Proliferation and Adhesion in Burn Wound Healing of Regenerated Silk Fibroin Membrane

Objective:To investigate the effect of nano-patterning modification on the cell proliferation and adhesion in burn wound healing of regenerated silk fibroin membrane.Methods:A total of 60 healthy SD mice were randomly divided into three groups:group A received treatment involving nano-patterning on the surface of regenerated silk fibroin membrane,group B received treatment with recombinant human epidermal growth factor gel,and group C received the same treatment with recombinant human epidermal growth factor gel,with 20 cases in each group.Wound healing,surface structure,protein adsorption,cell proliferation and adhesion were assessed at intervals of 5th,15th and 25th d after treatment.Results:The findings indicated that:(1)The duration and pace of wound healing in groups A and B surpassed those of group C,with group A exhibiting superior results compared to group B(P<0.05);(2)Histopathological analysis revealed a progressive increase in neovascularization and fibroblast count in wound tissue across the 5th,15th,and 25th days for all three groups,with group C exhibiting a higher count of neovascularization and fibroblasts in unhealed tissue compared to groups A and B.(3)The levels of basic calponin expression in group A and group B showed an increase on the 5th and 15th day,followed by stabilization on the 25th day.In group C,the expression of basic calponin was initially high on the 5th day,and then stabilized on the 15th and 25th day(P<0.05);(4)The expression of fibroblast proliferating cell nuclear antigen in the wound tissue of mice in all three groups peaked on the 15th day and subsequently declined.The expression of PCNA in group A and group B was higher than that in group C at each time point,with group A exhibiting higher levels than group B(P<0.05);(5)As wounds healed,there was a reduction in apoptotic cells within the wound tissues of mice across three groups,with group a exhibiting a lower count compared to groups B and C(P<0.05).Conclusion:Nanopatterning on the surface of regenerated silk fibroin membrane can enhance the biocompatibility of burn wound treatment and promote the proliferation and adhesion of reparative cells.