Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene seri...Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene series in workshop was determined by gas chromatography. As an internal exposure dose of benzene, the concentration of trans, trans-muconic acid （ttMA） was determined by high performance liquid chromatography. DNA was detected by modified single cell gel electrophoresis （SCGE）. Results The air concentrations of benzene, toluene and xylene at the workplace were 86.49±2.83 mg/m^3, 97.20±3.52 mg/m^3 and 97.45± 2.10 mg/m^3, respectively. Urinary ttMA in exposed group （1.040 ± 0.617 mg/L） was significantly higher than that of control group （0.819 ± 0.157 mg/L）. The percentage of head DNA, determined by modified SCGE method, significantly decreased in the exposed group （n=13, 70.18% ± 7.36%） compared with the control （n=16, 90.62% ± 2.94%）（P〈0.001）. Conclusion The modified SCGE method can be used to investigate the damage of sperm DNA. As genotoxin and reprotoxins, benzene had direct effect on the germ cells during the spermatogenesiss.展开更多
AIM:To study the effects of different Helicobacter pylori(H pylori) culture filtrates on growth of gastric epithelial cells. METHODS:Broth culture filtrates of H pylori were prepared. Gastric epithelial cells were tre...AIM:To study the effects of different Helicobacter pylori(H pylori) culture filtrates on growth of gastric epithelial cells. METHODS:Broth culture filtrates of H pylori were prepared. Gastric epithelial cells were treated with the filtrates,and cell growth was determined by growth curve and flow cytometry. DNA damage of gastric epithelial cells was measured by single-cell microgel electrophoresis. RESULTS:Gastric epithelial cells proliferated actively when treated by CagA-gene-positive broth culture filtrates,and colony formation reached 40%. The number of cells in S phase increased compared to controls. Comet assay showed 41.2% comet cells in GES-1 cells treated with CagA-positive filtrates(P < 0.05) . CONCLUSION:CagA-positive filtrates enhance the changes in morphology and growth characteristics of human gastric epithelial tumor cells. DNA damage maybe one of the mechanisms involved in the growth changes.展开更多
Objective:To evaluate retrospectively the effect of general anesthesia on DNA damage in the blood mononuclear cells(PBMCs) of surgical patients in order to provide evidence for a better nursing care during the procedu...Objective:To evaluate retrospectively the effect of general anesthesia on DNA damage in the blood mononuclear cells(PBMCs) of surgical patients in order to provide evidence for a better nursing care during the procedure.Methods:Clinical charts of 76 patients who underwent operation under general anesthesia and 76 healthy control subjects with documented results of DNA damage extent in PBMCs from the single-cell gel electrophoresis(SCGE) or cornel assay and scrum contents of superoxide dismutase(SOD) and malondialdehyde(MDA) from biochemical analyses were reviewed.The percentage of comet PBMCs and tail DNA and semm contents of SOD and MAD were analyzed by student t-test.Results:Compared with health) control subjects, generally anesthetized surgical patients had significantly higher%comet PBMCs and%tail DNA(P【0.05) and significantly lower serum concentrations of SOD(P【0.05) and significantly higher serum concentrations of MAD(P【0.05).Compared with levels before general anesthesia in surgical patients,%comet PBMCs,%tail DNA.and serum lexels of MAD were significantly higher(P【0.05 or 0.01).and semm levels of SOD were significantly lower(P【0.05).alter general anesthesia.Conclusions:General anesthesia during surgery causes a certain degree of hypoxia and PBMC damage.Particular attention should be paid to monitoring and maintenance of blood oxygen saturation in patients undergoing surgery under general anesthesia.展开更多
文摘Objective To assess the effect of benzene on sperm DNA damage ;Methods Twenty-seven benzene-exposed workers were selected as exposed group and 35 normal sperm donors as control group. Air concentration of benzene series in workshop was determined by gas chromatography. As an internal exposure dose of benzene, the concentration of trans, trans-muconic acid （ttMA） was determined by high performance liquid chromatography. DNA was detected by modified single cell gel electrophoresis （SCGE）. Results The air concentrations of benzene, toluene and xylene at the workplace were 86.49±2.83 mg/m^3, 97.20±3.52 mg/m^3 and 97.45± 2.10 mg/m^3, respectively. Urinary ttMA in exposed group （1.040 ± 0.617 mg/L） was significantly higher than that of control group （0.819 ± 0.157 mg/L）. The percentage of head DNA, determined by modified SCGE method, significantly decreased in the exposed group （n=13, 70.18% ± 7.36%） compared with the control （n=16, 90.62% ± 2.94%）（P〈0.001）. Conclusion The modified SCGE method can be used to investigate the damage of sperm DNA. As genotoxin and reprotoxins, benzene had direct effect on the germ cells during the spermatogenesiss.
基金The National Natural Science Foundation of China, No. 30271276
文摘AIM:To study the effects of different Helicobacter pylori(H pylori) culture filtrates on growth of gastric epithelial cells. METHODS:Broth culture filtrates of H pylori were prepared. Gastric epithelial cells were treated with the filtrates,and cell growth was determined by growth curve and flow cytometry. DNA damage of gastric epithelial cells was measured by single-cell microgel electrophoresis. RESULTS:Gastric epithelial cells proliferated actively when treated by CagA-gene-positive broth culture filtrates,and colony formation reached 40%. The number of cells in S phase increased compared to controls. Comet assay showed 41.2% comet cells in GES-1 cells treated with CagA-positive filtrates(P < 0.05) . CONCLUSION:CagA-positive filtrates enhance the changes in morphology and growth characteristics of human gastric epithelial tumor cells. DNA damage maybe one of the mechanisms involved in the growth changes.
文摘Objective:To evaluate retrospectively the effect of general anesthesia on DNA damage in the blood mononuclear cells(PBMCs) of surgical patients in order to provide evidence for a better nursing care during the procedure.Methods:Clinical charts of 76 patients who underwent operation under general anesthesia and 76 healthy control subjects with documented results of DNA damage extent in PBMCs from the single-cell gel electrophoresis(SCGE) or cornel assay and scrum contents of superoxide dismutase(SOD) and malondialdehyde(MDA) from biochemical analyses were reviewed.The percentage of comet PBMCs and tail DNA and semm contents of SOD and MAD were analyzed by student t-test.Results:Compared with health) control subjects, generally anesthetized surgical patients had significantly higher%comet PBMCs and%tail DNA(P【0.05) and significantly lower serum concentrations of SOD(P【0.05) and significantly higher serum concentrations of MAD(P【0.05).Compared with levels before general anesthesia in surgical patients,%comet PBMCs,%tail DNA.and serum lexels of MAD were significantly higher(P【0.05 or 0.01).and semm levels of SOD were significantly lower(P【0.05).alter general anesthesia.Conclusions:General anesthesia during surgery causes a certain degree of hypoxia and PBMC damage.Particular attention should be paid to monitoring and maintenance of blood oxygen saturation in patients undergoing surgery under general anesthesia.
