Single particle microbeam （SPM） is uniquely capable of delivering precisely the predefined number of charged particles to determined individual cells or sub-cellular targets in situ. It has been recognized as a powe...Single particle microbeam （SPM） is uniquely capable of delivering precisely the predefined number of charged particles to determined individual cells or sub-cellular targets in situ. It has been recognized as a powerful technique for unveiling ionization irradiation mechanisms of organism. This article describes some investigations on the irradiation quality of SPM of major world laboratories by means of Monte Carlo method based on dosimetry and microdosimetry. Those parameters are helpful not only to improve SPM irradiating cell experiments but also to study the biological effects of cells irradiated by SPM.展开更多
Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro, It is crucial to find a reference point...Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro, It is crucial to find a reference point that relates the microbeam＇s location to the microscope＇s plane, and align individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam, which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup.展开更多
Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioen...Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering （LIBB）, Chinese Academy of Sciences （CAS）, China. However there has been less research activities in this field concerning the original process of the interaction between low-energy ions and complicated organisms. To address this challenge, an in situ multi-dimensional quantitative fluorescence microscopy system combined with the CAS-LIBB single-particle microbeam II endstation is proposed. In this article, the rationale, logistics and development of many aspects of the proposed system are discussed.展开更多
Columbia University microbeam was constructed in 1993 and finished by the end of 1995. It is well established and used routinely to irradiate cells in a highly localized spatial region with a defined number of α-part...Columbia University microbeam was constructed in 1993 and finished by the end of 1995. It is well established and used routinely to irradiate cells in a highly localized spatial region with a defined number of α-particles. By using this probe, it is possible to study a number of radiobiological questions in ways that cannot be simulated by using conventional broad-field exposures. This report describes the development and current capabilities of the Columbia University microbeam, as well as the preliminary researches undertaken.展开更多
A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengi- neering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydro- ge...A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengi- neering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydro- gen ions, produced by a van de Graaff accelerator, in an en- ergy range of 2.0—3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is colli- mated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation proto- cols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.展开更多
基金the National Science Foundation for Distinguished Young Scholars of China(No.10225526)the Knowledge Innovation Program of the Chinese Academy Sciences(No.KSCX2-SW-324)the Foundation for University Key Teachers by the Ministry of Education of China(No.2005jq1135)
文摘Single particle microbeam （SPM） is uniquely capable of delivering precisely the predefined number of charged particles to determined individual cells or sub-cellular targets in situ. It has been recognized as a powerful technique for unveiling ionization irradiation mechanisms of organism. This article describes some investigations on the irradiation quality of SPM of major world laboratories by means of Monte Carlo method based on dosimetry and microdosimetry. Those parameters are helpful not only to improve SPM irradiating cell experiments but also to study the biological effects of cells irradiated by SPM.
文摘Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro, It is crucial to find a reference point that relates the microbeam＇s location to the microscope＇s plane, and align individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam, which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup.
文摘Single-particle microbeam as a powerful tool can open a research field to find answers to many enigmas in radiobiology. A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering （LIBB）, Chinese Academy of Sciences （CAS）, China. However there has been less research activities in this field concerning the original process of the interaction between low-energy ions and complicated organisms. To address this challenge, an in situ multi-dimensional quantitative fluorescence microscopy system combined with the CAS-LIBB single-particle microbeam II endstation is proposed. In this article, the rationale, logistics and development of many aspects of the proposed system are discussed.
文摘Columbia University microbeam was constructed in 1993 and finished by the end of 1995. It is well established and used routinely to irradiate cells in a highly localized spatial region with a defined number of α-particles. By using this probe, it is possible to study a number of radiobiological questions in ways that cannot be simulated by using conventional broad-field exposures. This report describes the development and current capabilities of the Columbia University microbeam, as well as the preliminary researches undertaken.
文摘A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengi- neering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined numbers of hydro- gen ions, produced by a van de Graaff accelerator, in an en- ergy range of 2.0—3.0 MeV, into an area smaller than that of the nucleus of an individual living cell. The beam is colli- mated by a borosilicate glass capillary that forms the beam-line exit. An integrated computer program recognizes the cells and locates them one by one over the microbeam exit for irradiation. We present technical details of the CAS-LIBB microbeam facility, particularly on the collimator, hardware, control program, as well as cell irradiation proto- cols available. Various factors contributing to the targeting and positioning precision are discussed along with accuracy measurement results.
