OBJECTIVE: To investigate the effects of the Sini San at different doses on each sleeping state[slow-wave sleep 1(SWS1), slow-wave sleep 2(SWS2), rapid-eye-movement(REM), wakefulness(W)] in insomnia rats and to identi...OBJECTIVE: To investigate the effects of the Sini San at different doses on each sleeping state[slow-wave sleep 1(SWS1), slow-wave sleep 2(SWS2), rapid-eye-movement(REM), wakefulness(W)] in insomnia rats and to identify its mode of ac-tion for improving sleep.METHODS: The insomnia rats were randomly divided into a high-, medium- or low-dose group of Sini San(equal to crude drug 8.8, 4.4, or 2.2 g/kg, respectively) for seven consecutive days.RESULTS: Compared with pre-administration,SWS2 was significantly increased after administration of the low dose. Compared with pre-administration, W was significantly decreased and SWS1,SWS2, and the total sleeping time(TST) were markedly increased after administration of the medium dose. Compared with pre-administration, W was significantly decreased and SWS1, SWS2, rapid-eye-movement sleep, and TST were significantly longer after administration of the high dose. The effects of Sini San on sleep-wake cycle are dose-dependent.CONCLUSION: The results suggest that Sini San extends SWS1 and SWS2, which increases the total sleeping time.展开更多
OBJECTIVE: To investigate the sedative and hypnotic activity of paeoniflorin and freeze-dried Sini San powder on mice and provide a reliable method for determining the pharmacodynamic material basis of Sini San.METHOD...OBJECTIVE: To investigate the sedative and hypnotic activity of paeoniflorin and freeze-dried Sini San powder on mice and provide a reliable method for determining the pharmacodynamic material basis of Sini San.METHODS: Male adult mice weighing 20-22 g were used in this study. Three experiments were carried out. Synergism with pentobarbital was used as an index for hypnotic effect. Loss of the righting reflex was used to determine the start of sleep.Sleep latency and sleeping time were recorded in each experiment.RESULTS: The coefficient of variation of the suprathreshold dose(55 mg/kg) was significantly lower than that of the threshold dose. The sleep latency of mice was significantly decreased, and the sleeping time of mice was significantly prolonged. The effects of paeoniflorin and Sini San on prolonging the sleeping time of mice induced by pentobarbital sodium were significantly stronger than those in the control group.CONCLUSION: Paeoniflorin produces significant sedative and hypnotic effects, and there is an obvious dose-effect relationship.展开更多
OBJECTIVE: To investigate the effects of Sini San and fluoxetine on the levels of central and peripheral 5-HT in a rat model of depression, and provide new insight into the treatment of depression with integrated Chin...OBJECTIVE: To investigate the effects of Sini San and fluoxetine on the levels of central and peripheral 5-HT in a rat model of depression, and provide new insight into the treatment of depression with integrated Chinese-Western Medicine.METHODS: A rat model of depression was established by chronic mild stress(CMS). Model rats received either Sini San, fluoxetine, a combination of the two drugs, or no drug treatment. Healthy naive rats were used as controls. Open field and sucrose preference tests were used to assess depression-like behavior. ELISA and immunohistochemistry were used to determine central and peripheral levels of 5-HT.RESULTS: In the group with no drug treatment,central 5-HT expression decreased while peripheral5-HT concentrations increased as CMS continued.Four weeks after CMS, Sini San alone was less effective in reducing depression-like behavior than fluoxetine alone or in combination with Sini San,but combined use was more effective than fluoxetine alone. Eight weeks after CMS, Sini San alone or in combination with fluoxetine was more effective in reducing depression-like behavior than fluoxetine alone. Furthermore Sini San and fluoxetine used alone or in combination notably increased central 5-HT expression and decreased peripheral 5-HT levels in the rat model.CONCLUSION:The results of the present study indicate that there is a synergistic action between the two medicines in the treatment of depression. Sini San exhibited a relatively long lag before its effects were observed; however, by eight weeks the Traditional Chinese Medicine appeared at least as effective as fluoxetine. We suggest that Sini San can replace fluoxetine in the later stages of depression treatment to minimize side effects observed with long-term fluoxetine administration.展开更多
【目的】初步探讨青少期应激结合成年后诱导抑郁大鼠海马CA1区细胞形态与5-羟色胺转运体(5-HTT)蛋白表达的变化及加味四逆散的干预作用。【方法】将132只Wistar大鼠随机分成空白组、模型组、加味四逆散组、氟西汀组,共4组,每组33只;组...【目的】初步探讨青少期应激结合成年后诱导抑郁大鼠海马CA1区细胞形态与5-羟色胺转运体(5-HTT)蛋白表达的变化及加味四逆散的干预作用。【方法】将132只Wistar大鼠随机分成空白组、模型组、加味四逆散组、氟西汀组,共4组,每组33只;组内再随机分为青少期、成年期、成年后3个小组,每小组11只。选取44、56、78 d 3个时间点作为青少期、成年期、成年后3个阶段的取材时间点。复制慢性不可预知应激(CUMS)大鼠模型。于第21~44天、第57~78天造模及给药,第44~55天正常喂养,不予造模及给药。观察各组大鼠的一般状况与体质量变化,采用苏木素—伊红(HE)染色法观察海马CA1区细胞形态,采用免疫组化染色法观察海马CA1区5-HTT阳性细胞的分布变化。【结果】模型组青少期、成年期、成年后大鼠一般状况较差,而经加味四逆散及氟西汀治疗后的大鼠均得到明显改善。各阶段的模型组大鼠体质量较同阶段的空白组显著降低(P<0.01);成年后,与空白组比较,其余3组体质量均显著降低(P<0.01),且3组间比较,差异无统计学意义(P>0.05)。在海马CA1区细胞形态表现方面,模型组青少期较多神经元萎缩、深染,胞核、胞浆界限不清,成年期及成年后呈进行性加重,大量神经元深染,胞核、胞浆界限不清,并有少量胶质细胞增生;而氟西汀组、加味四逆散组与同阶段模型组比较,神经元萎缩、深染减少。5-HTT表达结果显示,成年期、成年后模型组5-HTT的平均光密度值较同阶段的空白组显著降低(P<0.05或P<0.01),成年后加味四逆散组,成年期、成年后氟西汀组5-HTT的平均光密度值值较同阶段的模型组有显著升高(P<0.05或P<0.01)。【结论】在青少期受到CUMS的大鼠可产生抑郁样行为,成年后继续施加刺激诱导会进一步加重抑郁状态。抑郁大鼠海马内5-HTT改变可能是加味四逆散干预作用的重要通路。展开更多
基金Supported by Hippocampus Neural Coding Mechanism Research on Sini San Intervention Sleep Disorders of PTSD in Myospalax cansus from the National Natural Science Foundation(No.81460611)Study on Sini San for regulation of expression of proteins of drosophila brain of sleep deprivation of Gansu Province Natural Science Foundation(No.145RJZA076)+3 种基金Fundamental Research Funds for the Gansu Provincial Department of Finance Universities(No.2013-2)Mechanisms of hippocampal neurons based on Jiawei Sini San intervention coding mode PTSD sleep disordersMinistry of Education,Sini San for intervention of sleep deprivation in drosophila Based nano-2D-LC/MS technology of Science and Technology Key Project(No.212186)Proteomics and effective substance basic of Sini San for improving sleep of Gansu Province Natural Science Foundation(No.1010RJZA212)
文摘OBJECTIVE: To investigate the effects of the Sini San at different doses on each sleeping state[slow-wave sleep 1(SWS1), slow-wave sleep 2(SWS2), rapid-eye-movement(REM), wakefulness(W)] in insomnia rats and to identify its mode of ac-tion for improving sleep.METHODS: The insomnia rats were randomly divided into a high-, medium- or low-dose group of Sini San(equal to crude drug 8.8, 4.4, or 2.2 g/kg, respectively) for seven consecutive days.RESULTS: Compared with pre-administration,SWS2 was significantly increased after administration of the low dose. Compared with pre-administration, W was significantly decreased and SWS1,SWS2, and the total sleeping time(TST) were markedly increased after administration of the medium dose. Compared with pre-administration, W was significantly decreased and SWS1, SWS2, rapid-eye-movement sleep, and TST were significantly longer after administration of the high dose. The effects of Sini San on sleep-wake cycle are dose-dependent.CONCLUSION: The results suggest that Sini San extends SWS1 and SWS2, which increases the total sleeping time.
基金Supported by Gansu Province Finance colleges Fundamental Research Foundation (No.2013-2)Ministry of Education,Science and Technology Key Project (No.212186)and Gansu Province Natural Science Foundation (No.1010RJZA212)
文摘OBJECTIVE: To investigate the sedative and hypnotic activity of paeoniflorin and freeze-dried Sini San powder on mice and provide a reliable method for determining the pharmacodynamic material basis of Sini San.METHODS: Male adult mice weighing 20-22 g were used in this study. Three experiments were carried out. Synergism with pentobarbital was used as an index for hypnotic effect. Loss of the righting reflex was used to determine the start of sleep.Sleep latency and sleeping time were recorded in each experiment.RESULTS: The coefficient of variation of the suprathreshold dose(55 mg/kg) was significantly lower than that of the threshold dose. The sleep latency of mice was significantly decreased, and the sleeping time of mice was significantly prolonged. The effects of paeoniflorin and Sini San on prolonging the sleeping time of mice induced by pentobarbital sodium were significantly stronger than those in the control group.CONCLUSION: Paeoniflorin produces significant sedative and hypnotic effects, and there is an obvious dose-effect relationship.
基金Supported by a Grant from the National Basic Research Program of China(973 Program No.2011CB505106)
文摘OBJECTIVE: To investigate the effects of Sini San and fluoxetine on the levels of central and peripheral 5-HT in a rat model of depression, and provide new insight into the treatment of depression with integrated Chinese-Western Medicine.METHODS: A rat model of depression was established by chronic mild stress(CMS). Model rats received either Sini San, fluoxetine, a combination of the two drugs, or no drug treatment. Healthy naive rats were used as controls. Open field and sucrose preference tests were used to assess depression-like behavior. ELISA and immunohistochemistry were used to determine central and peripheral levels of 5-HT.RESULTS: In the group with no drug treatment,central 5-HT expression decreased while peripheral5-HT concentrations increased as CMS continued.Four weeks after CMS, Sini San alone was less effective in reducing depression-like behavior than fluoxetine alone or in combination with Sini San,but combined use was more effective than fluoxetine alone. Eight weeks after CMS, Sini San alone or in combination with fluoxetine was more effective in reducing depression-like behavior than fluoxetine alone. Furthermore Sini San and fluoxetine used alone or in combination notably increased central 5-HT expression and decreased peripheral 5-HT levels in the rat model.CONCLUSION:The results of the present study indicate that there is a synergistic action between the two medicines in the treatment of depression. Sini San exhibited a relatively long lag before its effects were observed; however, by eight weeks the Traditional Chinese Medicine appeared at least as effective as fluoxetine. We suggest that Sini San can replace fluoxetine in the later stages of depression treatment to minimize side effects observed with long-term fluoxetine administration.
文摘【目的】初步探讨青少期应激结合成年后诱导抑郁大鼠海马CA1区细胞形态与5-羟色胺转运体(5-HTT)蛋白表达的变化及加味四逆散的干预作用。【方法】将132只Wistar大鼠随机分成空白组、模型组、加味四逆散组、氟西汀组,共4组,每组33只;组内再随机分为青少期、成年期、成年后3个小组,每小组11只。选取44、56、78 d 3个时间点作为青少期、成年期、成年后3个阶段的取材时间点。复制慢性不可预知应激(CUMS)大鼠模型。于第21~44天、第57~78天造模及给药,第44~55天正常喂养,不予造模及给药。观察各组大鼠的一般状况与体质量变化,采用苏木素—伊红(HE)染色法观察海马CA1区细胞形态,采用免疫组化染色法观察海马CA1区5-HTT阳性细胞的分布变化。【结果】模型组青少期、成年期、成年后大鼠一般状况较差,而经加味四逆散及氟西汀治疗后的大鼠均得到明显改善。各阶段的模型组大鼠体质量较同阶段的空白组显著降低(P<0.01);成年后,与空白组比较,其余3组体质量均显著降低(P<0.01),且3组间比较,差异无统计学意义(P>0.05)。在海马CA1区细胞形态表现方面,模型组青少期较多神经元萎缩、深染,胞核、胞浆界限不清,成年期及成年后呈进行性加重,大量神经元深染,胞核、胞浆界限不清,并有少量胶质细胞增生;而氟西汀组、加味四逆散组与同阶段模型组比较,神经元萎缩、深染减少。5-HTT表达结果显示,成年期、成年后模型组5-HTT的平均光密度值较同阶段的空白组显著降低(P<0.05或P<0.01),成年后加味四逆散组,成年期、成年后氟西汀组5-HTT的平均光密度值值较同阶段的模型组有显著升高(P<0.05或P<0.01)。【结论】在青少期受到CUMS的大鼠可产生抑郁样行为,成年后继续施加刺激诱导会进一步加重抑郁状态。抑郁大鼠海马内5-HTT改变可能是加味四逆散干预作用的重要通路。
