赤霉素对大岩桐花蕾离体培养直接再生花芽的影响(简报)

植物经过一定时期的营养生长(或感受外界信号)后,就能产生成花刺激物.成花刺激物被运输到茎尖,诱导发生一系列的反应.随后其分生组织在一定时期内处于一个相对稳定的状态,即成花决定态.

大岩桐花萼切块离体培养直接再生花芽的形态观察(简报)

The morphological changes in the cultures of sepal segments in Sinningia speciosa Hiern were observed with Zeiss Stemi 2000-C Stereomicroscope from 0 to 65 days after culture in vitro. The light yellow globular protuberances were observed on the cut edge and the surface of sepal segments after culture for 24 days. Then the globular protuberances grew bigger gradually. A lot of floral buds on the surface of sepal segments formed after culture for 60 days. The morphological changes in the cultures of sepal segments were studied with light microscopy after culture for 0 to 30 days as well. The cells of tissues of sepal segments arranged regularly and no dividing cell was observed on the first day culture. There appeared some small meristematic centers of dividing cells on cut edge and the lower epidermis on the 7th day after culture. To the 20th day culture in vitro, the floral organ primordia were differentiated on the cut edge. On the 30th day culture, floral buds with petal, stamen primordia were observed. In addition, the morphological changes in the cultures of sepal segment were studied during the 14 to 30 days culture with scanning microscopy as well.

大岩桐SPY同源基因的克隆及其功能分析

以大岩桐幼叶为材料,利用RACE技术克隆到SPY基因的全长cDNA,命名为SsSPY(Genbank:ACF96937)。序列分析表明,SsSPY包含一个完整的ORF,长度为2805bp,编码934个氨基酸;含有SPY基因所共有的TPR保守结构域。通过蛋白序列比对,发现SsSPY与矮牵牛的PhSPY有82%的同源性,与拟南芥的SPY有81%的同源性,与大麦的HvSPY有74%的同源性。将SsSPY置于CaMV35S启动子控制下在烟草中异位表达,转基因烟草表现出株高、花芽数、分枝数显著增加,节间变长等新表型,表明SsSPY参与了转基因烟草株形的发育及花芽的分化。

大岩桐花瓣切块离体培养高频率花芽再生(英文)

该文报道了大岩桐花瓣切块离体培养再生花现象,花瓣切块再生花有两种方式:一种是仅再生花芽(命名为BF);另一种是既再生花芽也再生营养芽(命名为BF+V)。花芽再生的能力与光照、花芽大小及培养基中赤霉素和细胞分裂素浓度紧密相关。当培养基中含有1.0 mg/L GA3时,BA的添加会显著增加总花芽(BF+BF+V)的形成率,添加0.5 mg/L BA时,总花芽形成率达100%。在暗中培养时,BF达93.4%。不同大小花芽的花瓣再生花的能力不同,7 mm直径花芽的BF最高,达86.7%。同时,对花芽再生过程中花瓣切块的组织结构形态变化也进行了观察。