Treatment of Aged Skin with a pH 4 Skin Care Product Normalizes Increased Skin Surface pH and Improves Barrier Function: Results of a Pilot Study

The physiological skin surface pH is just below 5. With age the skin surface pH increases up to 6. An increased pH correlates with reduced barrier integrity/cohesion. The present pilot study assesses possible normalization of an increased skin surface pH of the elderly and improvement of barrier function via application of ≈pH 4.0 skin care products. Baseline skin surface pH was determined in elderly (80+ years old;n = 15) compared to middle aged adults (31 - 50 years old;n = 15). The effect of o/w emulsions at pH-values of 3.5, 4.0, 4.5 and 5.5 on the skin surface pH was determined in both groups. Further, the effect of a 4-week treatment with a pH 4.0 skin care product on the skin surface pH, skin hydration and barrier integrity was assessed. Thirteen elderly females were involved in this home-in-use test. Increased baseline skin surface pH of the elderly normalizes to the physiological pH of 4.5 - 5.0 over 7 hours after single application of o/w-emulsions with a given pH of 3.5 or 4.0. A 4 week treatment employing the pH 4.0 skin care product improves the epidermal barrier integrity of the elderly significantly (p = 0.005). Reduction of the increased baseline skin surface pH of the elderly is accompanied by improved epidermal barrier integrity. Skin care products for the elderly have to be adjusted in the pH range of 3.5 to 4.0.

Jonzac Thermal Spring Water Reinforces Skin Barrier Function of Human Skin and Presents a Soothing and Regenerating Effect

The skin is a formidable physical and biological barrier which communicates continuously with the outside of the body. And the stratum corneum, the outermost layer of human epidermis, plays a central role in the interaction between the cutaneous tissue and the external environment. The horny layer, and more generally the whole skin layers, avoid the penetration of harmful exogenous agents, produce molecules named anti-microbial peptides which impact the composition of the cutaneous microbiota, regulate the internal corporal temperature, avoid the water loss from the inside of the body and constitute an incredible efficient anti-oxidant network. Nevertheless, nowadays, the skin is more and more solicited by the different elements of the cutaneous exposome, including atmospheric pollution and solar radiations, which can cause a dramatic acceleration of the skin ageing process. As a consequence, due to the multifunctional protective role of the skin, during the recent decade the cosmetic industry invested massively in the development of new raw materials and end-products (dermo-cosmetics) able to preserve an optimal state of the skin regarding the external environment. Based on their physical-chemical properties thermal spring waters, which are extremely rich in inorganics ions, are interesting and powerful candidates to be part, as integral component, of new efficient dermo-cosmetic formulations dedicated to protect the skin from the external stimuli. The aim of the present work was to investigate and characterize the activity of Jonzac thermal spring water on the skin. Using different models, we proved for the first time that Jonzac thermal spring water reinforces the barrier function of the skin by modulating the expression of key markers including filaggrin and human beta defensin 2 on ex vivo human skin. The ex vivo and in vivo hydration activity, by Raman spectroscopy and corneometry respectively, has been also demonstrated. We have also shown that Jonzac thermal spring water ameliorates significantly the cutaneous microrelief in vivo. To conclude, we characterize the soothing effect of Jonzac thermal spring water by the analysis of histamine release in Substance P treated skin explants and by measuring the redness of the skin following UV exposure of the skin in vivo. We observed that both parameters decreased following a preventive treatment of the skin with Jonzac thermal spring water. Taken together our results indicate that Jonzac thermal spring water is a promising and powerful dermo-cosmetic which can be used to preserve an optimal state of the cutaneous tissue.

Gluconacetobacter hansenii GK-1 Ingestion for 8 Weeks Improves Skin Discomfort in Healthy Adults: A Randomized, Double-Blind, Placebo-Controlled Study

Gluconacetobacter hansenii GK-1 is an acid-resistant gram-negative bacterium used in vinegar brewing. Oral ingestion of GK-1 was previously reported to help maintain immunity and reduce nasal discomfort. Considering the suggested mechanism of action of activation of regulatory T cells via TLR4 to control Th1/Th2 balancing, GK-1 is also assumed to reduce skin discomfort secondary to immune reactions;however, this has not been validated in humans. Thus, we conducted a randomized, double-blind, placebo-controlled, parallel-group study on 100 healthy Japanese men and women (mean age, 47.6 ± 1.01 years) aged 20–64 years who consumed GK-1 (9 × 109 cells) daily for 8 weeks. Visual analog scale for overall, facial, arm, and leg skin discomfort was assessed before and after ingestion. The cumulative days of skin discomfort during the ingestion period were assessed. Compared with the placebo group, the G. hansenii GK-1 group had a significantly lower visual analog scale for overall and facial skin discomfort after 8 weeks and cumulative days of skin discomfort. Moreover, there were no adverse events attributable to G. hansenii GK-1. This study confirmed that oral ingestion of G. hansenii GK-1 contributed to skin integrity. The study protocol was preregistered at the Clinical Trials Registry System (registration no. UMIN000053005, December 7, 2023).

功能化自组装胶束增强氨甲环酸在皮肤色素沉着治疗中的作用

背景:氨甲环酸局部给药可用于改善皮肤色素沉着,但氨甲环酸极性大,局部给药时难以突破皮肤角质层屏障和细胞膜,皮下蓄积不易达到有效治疗浓度。目的:设计功能化自组装胶束,增强氨甲环酸抗皮肤色素沉着作用。方法:采用植物多酚衍生物表没食子儿茶素没食子酸棕榈酸酯与二甲双胍作为载体材料,通过氢键与静电作用自组装包载氨甲环酸,制备具有协同对抗色素沉着活性和增强药物渗透性能的载药自组装胶束;检测载药自组装胶束的纳米特性及稳定性,评价载药自组装胶束体外经皮渗透性能,考察载药自组装胶束的生物安全性和细胞水平效应。结果与结论:①载药自组装胶束的平均粒径为(176.27±5.23)nm,多分散系数为0.23±0.02,Zeta电位为(-25.67±0.98)mV,具有良好的稳定性,载药自组装胶束中氨甲环酸、二甲双胍质量浓度分别为(20.03±0.12),(6.67±0.08)mg/mL,载药自组装胶束对氨甲环酸和二甲双胍的载药量分别为(19.97±0.12)%,(6.65±0.08)%;②体外透皮实验结果显示,与氨甲环酸比较,载药自组装胶束具有更高的单位皮肤面积透过量和皮内贮留量,能渗透并扩散至皮肤更深层部位;③MTT检测结果显示,含氨甲环酸50-250μg/mL的载药自组装胶束对小鼠成纤维细胞与小鼠皮肤黑色素瘤细胞均无毒性作用,含氨甲环酸500μg/mL的载药自组装胶束对小鼠皮肤黑色素瘤细胞有轻微毒性作用;含氨甲环酸50-500μg/mL的载药自组装胶束未引发溶血反应,具有良好的生物安全性;④体外细胞培养结果显示,含氨甲环酸500μg/mL的载药自组装胶束可显著抑制小鼠皮肤黑色素瘤细胞的酪氨酸酶活性及黑色素释放,并且抑制作用强于单独的氨甲环酸溶液与二甲双胍溶液。结果表明,功能化自组装胶束能协同氨甲环酸抑制酪氨酸酶活性、减少黑色素合成,增强氨甲环酸抗皮肤色素沉着效应。

Skin Characteristics of Sites Predisposed to Pressure Ulcers among Bedridden Elderly Patients in Japan

Background: Previous studies have not specifically measured skin characteristics at common sites of pressure ulcers in high-risk elderly patients. Therefore, this study aimed to clarify the physiological skin characteristics at common pressure ulcer sites and their relationship with pressure ulcer risk and demographic/laboratory data in bedridden elderly Japanese patients. Methods: This study involved 55 elderly Japanese patients in a long-term care hospital and 25 female Japanese university students. Skin surface temperature, epidermal water content, transepidermal water loss, skin erythema/redness, skin elasticity, and skin thickness were measured using noninvasive devices. The sacral and both heel areas (sites predisposed to pressure ulcers) and mid-to-lower back area (control site) were observed. Results: Elderly patients showed a low epidermal water content at both heels (right heel, 14.8 ± 9.1 arbitrary units, AU.;left heel, 14.4 ± 8.3 AU). Transepidermal water loss remained acceptable at all sites in older patients despite the presence of dry skin (back, 7.1 ± 1.8 g/hm2;sacrum, 7.4 ± 3.0 g/hm2;right heel, 17.7 ± 7.1 g/hm2;left heel, 19.4 ± 8.1 g/hm2). Back (0.61 ± 0.13 AU) and sacral (0.67 ± 0.11 AU) skin elasticity and sacral skin thickness (0.97 ± 0.56 cm) were significantly lower than those of healthy young people (0.86 ± 0.04 AU, 0.87 ± 0.05 AU, and 2.27 ± 0.84 cm, respectively;p < 0.001 for all sites). Moderate positive correlations were observed between back skin elasticity and serum albumin level (r = 0.445, p < 0.001), and between sacral skin thickness and BMI (r = 0.506, p < 0.001) in older patients. Conclusions: Our findings showed that skin thickness and elasticity should be prioritized when evaluating pressure ulcer risk at the sacral region in bedridden elderly Japanese patients. Moreover, skin moisturization should be considered to minimize the risk at the heels in such patients.

A Dead Sea Water-Enriched Body Cream Improves Skin Severity Scores in Children with Atopic Dermatitis

Dead Sea (DS) mud and water are known for their unique composition of minerals, and for their therapeutic properties on inflammatory skin diseases. The objective of the study was to evaluate the efficacy of an emollient cream enriched with DS water in children with atopic dermatitis (AD). Eighty six AD children were randomized in a double-blind controlled study to receive twice-daily topical treatment with a body cream enriched with DS minerals (TP) compared to two types of control: 1) DM, DS minerals with lower DS water concentrations than TP, and 2) an emollient (E) with no DS minerals. Efficacy was assessed by a change in clinical skin severity scores: SCORing Atopic Dermatitis (SCORAD), investigator’s global assessment (IGA) and patient global assessment (PGA) as well as by objective physiological parameters: transepidermal water loss (TEWL), stratum corneum hydration (SCH), affected body surface area (BSA) and Objective Severity Assessment of Atopic Dermatitis (OSAAD). The total length of the trial was 12 weeks divided to 6 visits at weeks 0, 2, 4, 6, 8, 12. The study showed that both TP and DM creams improved OSAAD scores. Only TP improved TEWL and SCH. TP was the most effective regarding TEWL, SCH and OSAAD compared to DM and E. Treatment with E decreased more significantly IGA score compared to TP. Although within each treatment group significant improvements in SCH, BSA, SCORAD, IGA and PGA were observed, the reduction in BSA, SCORAD and PGA was not significantly different among the groups. Our results clearly show the benefits of TP as a leave on-skin emulsion enrich with DS water in terms of skin barrier function. Thus, TP can serve as an effective adjuvant treatment for AD skin as well as for its maintenance.

Protective Effects of a Novel Preparation Consists of Concentrated Dead Sea Water and Natural Plants Extracts against Skin Photo-Damage

Background: Exposure to solar UV rays has deleterious effects on skin appearance through physiological and structural alterations that eventually lead to skin photo-damage. Aims: To test the photo-protective effect of a novel preparation, Dead Sea Osmoter Concentrate (DSOC), containing high concentration of Dead Sea water. Methods: Human skin organ culture was used as a model to assess the biological effects of UVB irradiation and the protective effect of topical application of DSOC preparation. Skin pieces were analyzed for mitochondrial activity by Alamar blue (resazurin) assay, for apoptosis by caspase 3 assay, for detoxification by proteasome 20S sub-unit activity and for skin hydration by aquaporin 3 (AQP3) membrane channels expression. Human subjects were tested to evaluate the effect of DSOC Serum, a topical formula for facial skin, on skin hydration by a corneometer. Results: UVB irradiation induced cell apoptosis in the epidermis of skin organ cultures and decreased their proteasome activity and AQP3 expression. Topical application of DSOC significantly attenuated all these effects. In human subjects, an elevation in the intense skin moisture, which was higher relatively to subjects’ regular moisturizer, was observed. Moreover, DSOC Serum boosted the hydration effect together with regular moisturizer. Conclusions: The results clearly demonstrate that DSOC preparation can significantly improve the skin capability in coping with UVB rays stress in different cellular functions such as anti-apoptotic properties as well as detoxification and hydration recovery that can attenuate biological effects of skin photo-damage. Topical application of DSOC Serum had contributed to skin appearance by a strong hydration impact, also as a booster.

Recovery of sympathetic nerve function after lumbar sympathectomy is slower in the hind limbs than in the torso

Local sympathetic denervation by surgical sympathectomy is used in the treatment of lower limb ulcers and ischemia,but the restoration of cutaneous sympathetic nerve functions is less clear.This study aims to explore the recovery of cutaneous sympathetic functions after bilateral L2-4 sympathectomy.The skin temperature of the left feet,using a point monitoring thermometer,increased intraoperatively after sympathectomy.The cytoplasm of sympathetic neurons contained tyrosine hydroxylase and dopamineβ-hydroxylase,visualized by immunofluorescence,indicated the accuracy of sympathectomy.Iodine starch test results suggested that the sweating function of the hind feet plantar skin decreased 2 and 7 weeks after lumbar sympathectomy but had recovered by 3 months.Immunofluorescence and western blot assay results revealed that norepinephrine and dopamineβ-hydroxylase expression in the skin from the sacrococcygeal region and hind feet decreased in the sympathectomized group at 2 weeks.Transmission electron microscopy results showed that perinuclear space and axon demyelination in sympathetic cells in the L5 sympathetic trunks were found in the sympathectomized group 3 months after sympathectomy.Although sympathetic denervation occurred in the sacrococcygeal region and hind feet skin 2 weeks after lumbar sympathectomy,the skin functions recovered gradually over 7 weeks to 3 months.In conclusion,sympathetic functional recovery may account for the recurrence of hyperhidrosis after sympathectomy and the normalization of sympathetic nerve trunks after incomplete injury.The recovery of sympathetic nerve function was slower in the limbs than in the torso after bilateral L（2-4） sympathectomy.

Olfactory ensheathing cell transplantation improves sympathetic skin responses in chronic spinal cord injury

Forty-three patients with chronic spinal cord injury for over 6 months were transplanted with bryonic olfactory ensheathing cells, 2-4 × 106, into multiple sites in the injured area under the sur-gical microscope. The sympathetic skin response in patients was measured with an electromyo-graphy/evoked potential instrument 1 day before transplantation and 3-8 weeks after trans-tion. Spinal nerve function of patients was assessed using the American Spinal Injury Association impairment scale. The sympathetic skin response was elicited in 32 cases before olfactory en-sheathing celltransplantation, while it was observed in 34 cases after transplantation. tantly, sympathetic skin response latency decreased significantly and amplitude increased cantly after transplantation. Transplantation of olfactory ensheathing cells also improved American Spinal Injury Association scores for movement, pain and light touch. Our findings indicate that factory ensheathing celltransplantation improves motor, sensory and autonomic nerve functions in patients with chronic spinal cord injury.

黄芩苷调节cAMP/PKA/CREB信号通路对湿疹大鼠皮肤屏障功能的影响

目的探讨黄芩苷(BA)调节环磷酸腺苷(cAMP)/蛋白激酶A(PKA)/cAMP反应元件结合蛋白(CREB)通路对湿疹大鼠皮肤屏障功能的影响。方法将SD大鼠随机分为对照组(NC组)、Model组、低剂量BA组(BA-L组,25 mg/kg)、中剂量BA组(BA-M组,50 mg/kg)、高剂量BA组(BA-H组,100 mg/kg)、泼尼松组(PNS组,25 mg/kg)、BAH+cAMP抑制剂(SQ22536)组(100 mg/kg+2.13 mg/kg)、BA-H+PKA抑制剂(H-89)组(100 mg/kg+5 mg/kg),每组12只。除NC组外,其余组大鼠均构建湿疹大鼠模型。建模成功2 d后,分组进行给药处理。检测湿疹面积及严重度指数(EASI)评分、经皮肤水分流失量(TEWL)、角质层含水量(WCSC)变化;酶联免疫吸附试验(ELISA)检测大鼠血清中免疫球蛋白E(IgE)、干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平及大鼠背部受试区皮损组织中c AMP蛋白表达;HE染色检测大鼠背部受试区皮损组织病理变化;Western blot检测大鼠背部受试区皮损组织中水通道蛋白3(AQP3)、cathelicidin相关抗菌肽(CRAMP)、p-PKA、p-CREB蛋白表达。结果与NC组比较,Model组大鼠背部受试区皮损组织病理损伤严重,EASI评分、TEWL、IgE、IL-4水平升高,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平降低(P<0.05)。与Model组比较,BA-L组、BA-M组、BA-H组、PNS组大鼠背部受试区皮损组织病理损伤减轻,EASI评分、TEWL、IgE、IL-4水平降低,WCSC、IFN-γ水平、AQP3、CRAMP、cAMP、p-PKA、p-CREB蛋白水平升高(P<0.05);且BA-L组、BA-M组、BA-H组上述指标变化呈剂量依赖性。SQ22536或H-89减弱了高剂量BA对湿疹大鼠皮肤屏障功能的改善作用。结论BA可能通过激活cAMP/PKA/CREB信号通路改善湿疹大鼠皮肤屏障功能。

A FINITE-ELEMENT FORMULATION OF HEATING RATE AND SKIN FRICTION ON THE BASIS OF CONSERVAION LAWS

In the framework of the finite element method (FEM), a prediction method for the heating rate and the skin friction on a body surface is presented by using the energy and momentum conservation equations respectively. Meanwhile, a brief analysis is made of the role the weighted functions play in the present work.

Effectiveness of the Cussons Baby Sensicare Range of Products on Skin Moisturization, Softness and Suppleness of the Skin, Trans Epidermal Water Loss and Dermoprotection

OBJECTIVE: Baby skin differs significantly from adult skin and requires special care. The Cussons Baby Sensicare Range (Sensicare) of products has been specifically formulated for baby skin and a range of standard cosmetic clinical tests were conducted in adult females with dry skin to evaluate the effectiveness of the range on skin moisturization and barrier function. METHODS: The studies were within subject, controlled, single blind studies. For the Sensicare Body Wash skin moisturization (Corneometer) was measured before application and after 7 and 14 days of twice daily use. For the Sensicare Moisturizing Lotion and Sensicare Soothing Cream skin moisturization was measured before application and at 2, 4, 6, 8 and 24 hours after a single application;skin elasticity (Cutometer) and transepidermal water loss (TEWL, Tewameter) were assessed before and after 14 days of twice daily use;and skin erythema (Mexameter) before induction with sodium lauryl sulphate (SLS), and at 30 mins, 24 and 48 hours after patch removal. Changes from baseline and changes versus control were evaluated. RESULTS: Thirty to thirty-five subjects completed each study. Skin moisture content was significantly increased from baseline for all Cussons Baby Sensicare Products (p < 0.05 for Hair & Body Wash;and p < 0.001 for Cream & Lotion). Changes from baseline in skin elasticity were significantly superior to control (unperfumed liquid soap) after 14 days twice daily use of the Lotion, and Cream (p < 0.05). There were no significant changes from baseline or significant differences from control in TEWL for the Lotion, or Cream. The increases in the average skin erythema index were significantly smaller for the Lotion, and Cream 30 minutes following SLS patch removal (p < 0.05 v sterile water, p < 0.001 v no treatment for both products). All the Sensicare products were well tolerated. CONCLUSION: These studies support the use of the Cussons Baby Sensicare range of products for new born, sensitive and eczema prone skin.

Sympathetic skin response in patients with myasthenia gravis: A comparative analysis

BACKGROUND： The examination of sympathetic skin response is an important index for assessing the autonomic nerve function, and patients with myasthenia gravis are always accompanied by dysautonomia. Therefore, it will be important to know whether sympathetic skin response can be used as the index for the clinical evaluation of myasthenia gravis. OBJECTIVE： To investigate the diagnostic value of sympathetic skin response in the damage of autonomic nerve function of patients with myasthenia gravis. DESIGN： A case-controlled comparative observation. SETTING： Department of Neurology and Room of Nerve Electromyogram, the Affiliated Hospital of North Sichuan Medical College. PARTICIPANTS： Thirty outpatients or inpatients with myasthenia gravis were selected from the Department of Neurology, the Affiliated Hospital of North Sichuan Medical College from May 2006 to May 2007, including 9 males and 21 females, aged 8 - 72 years with a mean age of （28 ± 5） years old. They were all accorded with the diagnostic standards of myasthenia gravis, accompanied by different severity of autonomic nerve symptoms, including poor skin nutrition, sweating of hands and feet, pyknocardia, persistent hypotension, abdominal pain, constipation, etc. They all had not taken any drug affecting the autonomic nerve function before the examination. Informed consents were obtained from all the patients. Meanwhile, 30 healthy physical examinees were enrolled as the normal control group, including 10 males and 20 females, aged 10 - 75 years with a mean age of （31 ±5） years old. Approval was obtained from the hospital ethic committee. METHODS： After admission, the patients were examined with sympathetic skin response using DANTEC keypoint 2.0 electromyography evoked potential apparatus （Danmark）. The changes of the latency and wave amplitude of sympathetic skin response were observed. The subjects in the normal control group were examined with the same methods at physical examination. Abnormality was judged by the disappearance of wave form, latency longer than that in the normal control group by Mean＋2.5SD, or wave amplitude lower than the average value in the normal control group by 50%. MAIN OUTCOME MEASURES： The results of the latency and wave amplitude of sympathetic skin response were compared between the patients with myasthenia gravis and normal controls. RESULTS： All the 30 patients with myasthenia gravis and 30 healthy physical examinees were involved in the final analysis of results. There were no significant differences between the left and right upper and lower limbs in both the myasthenia gravis group and normal control group （P 〉 0.05）. In the myasthenia gravis group, the abnormal rate of sympathetic skin response was 37% （11/30）, the latency was prolonged and the wave amplitude was decreased as compared with those in the normal control group, and there were significant differences （P 〈 0.01）. CONCLUSION： Sympathetic skin response can be used as an electrophysiological index for judging the damages of autonomic nerve function in patients with myasthenia gravis.

微针导入重组Ⅲ型人源化胶原蛋白在皮肤屏障功能修复中的应用效果

目的探究微针导入重组Ⅲ型人源化胶原蛋白治疗因皮肤炎性衰老导致的皮肤屏障功能下降的有效性及安全性。方法选取2020年6月至2021年5月在本院接受面部皮肤治疗的30例患者作为研究对象,采用随机数字表法将其分为对照组(重组Ⅲ型人源化胶原蛋白)与治疗组(微针导入重组Ⅲ型人源化胶原蛋白),各15例。治疗后定期对患者进行随访,分别进行主观疗效评价及客观疗效评价。结果治疗后4、8、12周,治疗组的满意度显著高于对照组(P<0.05)。治疗后,治疗组的皮肤皱纹、纹理、红区、毛孔、弹性、皮肤经皮失水及皮肤含水量改善明显(P<0.05);对照组治疗后油脂改善明显(P<0.05)。治疗后12周,治疗组的总症状评分显著低于对照组(P<0.05)。结论微针导入重组Ⅲ型人源化胶原蛋白对皮肤修复有较好的疗效,可增强皮肤屏障功能,为改善皮肤屏障功能、治疗因皮肤屏障受损引起的炎性衰老提供了新选择。

拉曼光谱研究表面活性剂对皮肤刺激和皮肤防护的体内分子机制

为了更好地研究表面活性剂对皮肤损伤和皮肤防护的分子机制,需要研究人(内源性物质和结构)、物质(刺激物和抗刺激活性物)、以及人与物质的相互作用。然而,在人体上分子水平的机制研究报道很少。共聚焦拉曼光谱可以开展此类研究,因其可以在体、实时和非侵入式地测定角质层中的某些内源性或外源性物质,并分析角质层结构。文章以十二烷基硫酸钠(SDS)作为刺激剂,通过共聚焦拉曼光谱扫描志愿者前臂屈侧皮肤12μm深度内,定性和半定量地测量SDS经皮吸收的相对量和深度,以及SDS“入侵”时皮肤脂质相对含量和结构的变化。结果表明,SDS经皮吸收进入角质层,导致角质层脂质流失和脂质有序性降低。据报道刺激物的进入量和深度是影响皮肤刺激程度的两个主要因素,文章首次在人体上通过SDS给予证实;此外,文章首次发现SDS引起的人体皮肤屏障损伤的第三个因素,我们称之为“刺激传导网络”,即SDS对皮肤的损伤不仅局限于其所到之处,“刺激传导网络”可以将刺激损伤传递到SDS未到达的更深更远处。通过拉曼光谱对皮肤内部进行研究,并结合体外手段例如3D皮肤等,结果一致,进而提出了“现实世界发生的”SDS诱导皮肤损伤的分子学机制。根据该机制设计的表面活性剂拮抗剂简称ASF,数据显示,可以有效阻挡SDS的经皮吸收,进而有效阻止皮肤脂质流失和脂质有序性的降低,对皮肤屏障的保护效果很好,为表面活性剂在人体刺激机制以及保护机制提供进一步佐证。

枇杷叶三萜酸联合重组人表皮生长因子对激素依赖性皮炎豚鼠皮肤屏障功能的修复及免疫失衡的影响

目的 观察枇杷叶三萜酸(TAL)联合重组人表皮生长因子(rh-EGF)对激素依赖性皮炎(HDD)豚鼠模型皮肤屏障功能修复及免疫失衡的影响。方法 选取40只无特定病原(SPF)级豚鼠,随机分为对照组、模型组、rh-EGF组、TAL组和联合组,每组各8只。除对照组外,其他各组涂抹0.05%卤米松乳膏,连续45 d,构建HDD模型。构建成功后,rh-EGF组涂抹rh-EGF凝胶,TAL组涂抹TAL溶液,联合组涂抹TAL和rh-EGF凝胶,对照组和模型组涂抹生理盐水,共15 d。记录各组临床症状并打分;检测经皮水分丢失(TEWL)、角质层含水量(WCSC)和皮脂量(SC);HE染色观察各组豚鼠皮肤组织病理变化;采用酶联免疫吸附测定(ELISA)法检测总免疫球蛋白E(IgE)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)水平。结果 与对照组比较,模型组症状明显、皮肤组织损坏严重,TEWL增加,WCSC和SC减少;免疫因子Ig E和IL-4水平升高、IFN-γ水平降低(P<0.05)。与模型组比较,rh-EGF组、TAL组和联合组用药后临床症状减轻、皮肤组织损伤不明显;TEWL减少,WCSC和SC增加,免疫因子IgE和IL-4水平降低、IFN-γ水平升高(P<0.05)。且联合组与rh-EGF组和TAL组比较,作用效果更加明显(P<0.05)。TAL组和联合组各指标比较,差异无统计学意义(P>0.05)。结论 TAL联合rh-EGF可有效缓解HDD豚鼠的临床症状,改善皮肤细胞损伤,修复皮肤屏障功能,提升免疫能力。

健脾祛风汤联合皮炎洗剂治疗特应性皮炎疗效研究

目的:研究健脾祛风汤联合皮炎洗剂治疗特应性皮炎的效果及对免疫球蛋白E(IgE)和白介素-4(IL-4)的影响。方法:收集120例特应性皮炎患者临床资料,并开展回顾性对比研究。观察组60例,行健脾祛风汤联合皮炎洗剂;对照组60例,行皮炎洗剂干预。比较两组患者治疗效果,记录两组患者治疗前后皮肤屏障功能[皮脂含量、角质层含水量及经皮水分丢失(TEWL)含量]、血清因子(IL-4、IgE)水平及生活质量评分。结果:观察组患者整体疗效较对照组显著提高(P<0.05)。治疗后观察组患者皮脂含量和角质层含水量均高于对照组,TEWL低于对照组,差异有统计学意义(P<0.05)。治疗后两组患者血清IL-4和IgE较治疗前均显著降低,且观察组低于对照组,差异有统计学意义(P<0.05)。治疗后两组皮肤病生活质量指数(DLQI)和特应性皮炎积分(SCORAD)较治疗前均显著降低,且观察组低于对照组(P<0.05)。结论:健脾祛风汤联合皮炎洗剂用于特应性皮炎效果显著,有助于保护皮肤屏障功能,减轻临床症状,改善患者生活质量。

强脉冲光联合类人胶原蛋白敷料治疗面部色素沉着的疗效及相关测评指标变化评析

目的:探究强脉冲光联合类人胶原蛋白敷料治疗面部色素沉着的疗效以及对VISIA系统色素沉着评分和表皮油脂量、TEWL的影响。方法:选取从2021年1月-2022年6月在笔者医院收治的100例女性面部色素沉着患者,将所有患者按随机数字表法分配为对照组(n=50)、观察组(n=50),对照组使用强脉冲光治疗,观察组在对照组的基础上联合类人胶原蛋白敷料治疗;比较两组患者的疗效、VISIA系统色素沉着评分、皮肤屏障功能:包括表皮含水量、表皮油脂含量和经皮水分丢失量(TEWL)、SAS、DLQI评分的变化情况和不良反应的发生情况。结果:观察组患者治疗后总有效率(88.00%,44/50)高于对照组(P<0.05);与治疗前相比,治疗后观察组和对照组的VISIA系统色素沉着评分、TEWL、SAS、DLQI评分显著降低,表皮含水量、表皮油脂含量显著升高(P<0.05),治疗后与对照组相比,观察组的VISIA系统色素沉着评分、TEWL、SAS、DLQI评分显著降低,表皮含水量、表皮油脂含量显著升高(P<0.05);不良反应发生率,观察组和对照组比较,差异无统计学意义(P>0.05)。结论:强脉冲光联合类人胶原蛋白敷料治疗面部色素沉着的效果显著,可以促进色素沉着消退,改善皮肤屏障功能,改善患者生活质量和心理状态,且安全性较高。

内源性大麻素系统对皮脂腺功能的调控作用研究进展

皮脂腺(sebaceous glands)的基本生理功能是产生皮脂以滋润皮肤和毛发,加强皮肤的屏障功能,控制皮肤表面微生物的生长,并参与调节皮肤炎症和免疫应答。皮脂腺的功能失调在痤疮、脂溢性皮炎、特应性皮炎等常见皮肤病的发病机制中起着重要作用。内源性大麻素系统(endocannabinoid system)是一种复杂的稳态调控信号网络,由内源性大麻素、大麻素受体、以及参与内源性大麻素转运、合成和降解的酶组成。近年的研究发现内源性大麻素系统的各组分在皮脂腺中都有表达,并且参与皮脂腺功能的调控,表明其在皮脂腺功能障碍相关皮肤病防治中具有潜在应用前景。本文就内源性大麻素系统对皮脂腺功能的调控作用研究进展进行综述。

养肤润肠汤治疗非小细胞肺癌表皮生长因子受体抑制剂相关性皮疹临床观察

目的分析养肤润肠汤治疗非小细胞肺癌表皮生长因子受体抑制剂相关性皮疹患者的临床疗效。方法选择2020年5月至2022年5月于该院治疗的非小细胞肺癌患者表皮生长因子受体抑制剂相关性皮疹患者120例为研究对象,采用随机数字表法分为西医组和养肤润肠汤组,每组60例。西医组采用常规西医治疗,养肤润肠汤组在常规西医治疗同时给予养肤润肠汤治疗,均治疗1个月。检测两组治疗前、治疗后肿瘤坏死因子-α(TNF-α)、胸腺基质淋巴细胞生成素(TSLP)、白细胞介素-1β(IL-1β)、趋化因子27(CXCL27)水平,检测两组角质层含水量、皮肤油脂含量、经皮水分丢失量,对两组皮疹面积、皮疹疼痛、皮疹瘙痒进行评分,记录皮疹首次改善时间,比较治疗前、治疗后两组皮疹分级、证候评分、皮肤病生活质量指数量表(DLQI)评分,比较两组治疗后临床疗效。结果治疗前,两组TNF-α、TSLP、CXCL27、IL-1β水平,皮肤油脂含量、角质层含水量、经皮水分丢失量,皮疹瘙痒、皮疹疼痛、皮疹面积评分,以及中医证候、DLQI评分比较,差异均无统计学意义(P>0.05)。与治疗前比较,治疗后两组以上指标均得到改善,差异均有统计学意义(P<0.05)。治疗后,养肤润肠汤组TNF-α、TSLP、CXCL27、IL-1β水平低于西医组,皮肤油脂含量、角质层含水量均高于西医组,经皮水分丢失量低于西医组,皮疹瘙痒、皮疹疼痛、皮疹面积评分均低于西医组,皮疹首次改善时间短于西医组,差异均有统计学意义(P<0.05)。治疗后,养肤润肠汤组皮疹分级较西医组好,差异有统计学意义(Zc=1.719,P=0.037)。养肤润肠汤组总有效率高于西医组,差异有统计学意义(χ^(2)=5.886,P=0.015)。结论给予非小细胞肺癌表皮生长因子受体抑制剂相关性皮疹患者养肤润肠汤治疗,可减少TSLP水平,抑制炎症反应,改善皮疹分级,提升其生活质量、皮肤屏障功能及临床疗效。