AIM:To investigate the effects of recombinant human epithelium growth factor ( rhEGF) applied to skin graft.METHODS:96 cases between February 2000 and December 2001,were treated.During the operation,After scar removed...AIM:To investigate the effects of recombinant human epithelium growth factor ( rhEGF) applied to skin graft.METHODS:96 cases between February 2000 and December 2001,were treated.During the operation,After scar removed and skin grafted,the rhEGF was injected under the skin graft.80 cases without injection of rhEGF were made as contrast.Ten days later,the area of survived skin was measured and the livability of skin was calculated. RESULTS: The skin livability of cases with in jection of rhEGF was (90.67±10.02)% and the skin livability of contrast cases was(76.85±8.35)%. There axisted evident differences between them(P< 0.01).CO NCLUSION:The rhEGF was an effective method for increasing livability of skin gra ft.展开更多
Skin toxicity is a common symptom of anti-epidermal rowth factor receptor(EGFR) antibody treatment and is also a predictive marker of its efficacy in colorectal cancer patients. However, severe skin disorders induced ...Skin toxicity is a common symptom of anti-epidermal rowth factor receptor(EGFR) antibody treatment and is also a predictive marker of its efficacy in colorectal cancer patients. However, severe skin disorders induced by such antibodies negatively impact on the quality of life of patients and decreases drug compliance during treatment. If we can predict the high-risk group susceptible to severe skin toxicity before treatment, we can undertake the early management of any arising skin disorders and formulate a more accurate prognosis for anti-EGFR antibody treatment. Previous studies have identified molecular markers of skin toxicity induced by anti-EGFR antibody, such as EGFR polymorphisms, the expression of inflammatory chemokines and serum levels of EGFR ligands. A clinical trial was undertaken involving the escalation of cetuximab doses, guided by the grade of skin toxicity observed, such as no or low-grade, in metastatic colorectal cancer(the EVEREST study). The dose escalation of cetuximab was confirmed by a safety profile and had the tendency to achieve a higher response rate in KRAS wild-type patients. A large, prospective randomized trial is now ongoing(EVEREST 2) and the results of this trial may contribute to personalized medicine in KRAS wild-type colorectal cancer patients.展开更多
Objective To scrutinize the effect of growth factors resulting from the intermingled skin graft onwound healing. Methods By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF andbFGF of autografts and ...Objective To scrutinize the effect of growth factors resulting from the intermingled skin graft onwound healing. Methods By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF andbFGF of autografts and allografts on burnt rats were quantitatively analysed. Results (1) mRNA expressions ofall the four growth factors mentioned above were found both in allografts and autografts, the expressions beinghigher in autografts than allografts; (2) In autografts, TGFα expression began to rise from second post transplantday (D2), reaching a peak on D18,. TGFβ1 expression gained a high level on D2 and had itS peak on D18; PDGFexpression had its peak on D4, rebounding on D25; bFGF expression, exceedingly high on D2, dropped andrebounded in the same manner as in PDGF; (3) In the early stage of the wound healing, mRNA expression of thefour growth factors was lower in allografts, rising up in varying degrees thereafter. Conclusion (1) Graftsurvival took place both in autografts and allografts, with the former pederming better than the latter; (2) Thecombining action of growth factors that reached the affected area through autosecretion and bysecretion probablypromoted the healing of the burn wound, following signal transmission channels; (3) Rejection, which occurred inallogralts, was responsible for the decreasing viability of allografts and their replacement by the host skin.展开更多
This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC...This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC), poly(γ-glutamic acid) (PGA), and argentine (Arg). High-molecular weight HA aqueous solution, hydrolyzed low-molecular weight HA aqueous solution, and heat- denatured Col aqueous solution were mixed, into which each aqueous solution containing EGF, VC, GC, PGA, or Arg were added, followed by freeze-drying to obtain a spongy EGF-incorporating skin care product (EGF-skin care product). In order to evaluate the first efficacy of EGF, fibroblast proliferation was assessed after 6 days of cultivation in the conditioned medium prepared by dissolving EGF-skin care product in a conventional culture medium. The fibroblast densities increased more effectively in conditioned medium with EGF than in control medium without EGF. In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. A fibroblast-incorporating Col gel sheet (cultured dermal substitute: CDS) was elevated to the air- medium interface, onto which a spongy sheet of EGF-skin care product was placed and cultured for 7 days. The condition covered with or without EGF-skin care product is divided into (+) EGF or (-) EGF, respectively. Fibroblasts in the CDS released 3.7 times more VEGF and 25 times more HGF in (+) EGF compared with (-) EGF. In another experiment, an aqueous solution of EGF-skin care product was added onto CDS and cultured for 7 days. Aqueous solutions were prepared and stored at 4°C or 37°C for a different period of 1 day, 2 weeks, and 4 weeks, respectively. Fibroblasts in CDS under different condition released similar amount of VEGF and HGF. This result indicated that the efficacy of EGF was maintained even after preservation at 37°C for 4 weeks. These findings suggest that EGF-skin care product can be used on damaged skin surface by placing its spongy sheet or its solution.展开更多
目的:NOD样受体热蛋白结构域相关蛋白6(NOD-like receptor thermal protein domain associated protein 6,NLRP6)是新发现的寡聚化核苷酸结合结构域样受体家族成员,广泛表达于肠道、肝脏、肾脏、脾脏和肌肉等组织器官,在炎症、焦亡和自...目的:NOD样受体热蛋白结构域相关蛋白6(NOD-like receptor thermal protein domain associated protein 6,NLRP6)是新发现的寡聚化核苷酸结合结构域样受体家族成员,广泛表达于肠道、肝脏、肾脏、脾脏和肌肉等组织器官,在炎症、焦亡和自噬等多种生物过程发挥广泛的调节作用。近期研究表明,NLRP6在应激条件下对多种组织器官的疾病表型具有重要影响。然而,NLRP6对组织器官生长发育的影响尚未可知。方法:采用CRISPR/Cas9基因编辑技术构建NLRP6基因敲除小鼠,饲养并记录小鼠的生长和繁殖情况。将小鼠的脾、肝、心、肾、脑、四肢和后背皮肤等组织器官进行解剖和切片染色,以评估NLRP6基因敲除对实质器官的宏观发育影响和对组织结构的微观影响。结果:在自然状态下,NLRP6基因敲除缩短了雄鼠的性成熟期并使成年雄鼠的睾丸发生不可逆的破溃与萎缩。在四肢发育上,NLRP6基因敲除诱导成年雄鼠后肢横纹肌断裂,导致后肢明显萎缩。在脾脏发育上,NLRP6基因敲除不仅显著增加了雄鼠的脾脏体积(P<0.01)还诱导雄鼠脾脏出现炎性细胞浸润。在后背皮肤上,NLRP6基因敲除引发雄鼠后背皮肤出现明显的溃疡损伤、胶原纤维增生和炎性细胞浸润。结论:在自然生长发育条件下,NLRP6基因敲除选择性影响小鼠的生殖器发育与性成熟期、后肢肌肉发育、脾脏大小及其炎症免疫状态以及后背皮肤的结构完整性,而且这一作用具有明显的雄激素依赖性。展开更多
文摘AIM:To investigate the effects of recombinant human epithelium growth factor ( rhEGF) applied to skin graft.METHODS:96 cases between February 2000 and December 2001,were treated.During the operation,After scar removed and skin grafted,the rhEGF was injected under the skin graft.80 cases without injection of rhEGF were made as contrast.Ten days later,the area of survived skin was measured and the livability of skin was calculated. RESULTS: The skin livability of cases with in jection of rhEGF was (90.67±10.02)% and the skin livability of contrast cases was(76.85±8.35)%. There axisted evident differences between them(P< 0.01).CO NCLUSION:The rhEGF was an effective method for increasing livability of skin gra ft.
文摘Skin toxicity is a common symptom of anti-epidermal rowth factor receptor(EGFR) antibody treatment and is also a predictive marker of its efficacy in colorectal cancer patients. However, severe skin disorders induced by such antibodies negatively impact on the quality of life of patients and decreases drug compliance during treatment. If we can predict the high-risk group susceptible to severe skin toxicity before treatment, we can undertake the early management of any arising skin disorders and formulate a more accurate prognosis for anti-EGFR antibody treatment. Previous studies have identified molecular markers of skin toxicity induced by anti-EGFR antibody, such as EGFR polymorphisms, the expression of inflammatory chemokines and serum levels of EGFR ligands. A clinical trial was undertaken involving the escalation of cetuximab doses, guided by the grade of skin toxicity observed, such as no or low-grade, in metastatic colorectal cancer(the EVEREST study). The dose escalation of cetuximab was confirmed by a safety profile and had the tendency to achieve a higher response rate in KRAS wild-type patients. A large, prospective randomized trial is now ongoing(EVEREST 2) and the results of this trial may contribute to personalized medicine in KRAS wild-type colorectal cancer patients.
文摘Objective To scrutinize the effect of growth factors resulting from the intermingled skin graft onwound healing. Methods By means of RT-PCR technique, mRNA expressions of TGFα, TGFβ1, PDGF andbFGF of autografts and allografts on burnt rats were quantitatively analysed. Results (1) mRNA expressions ofall the four growth factors mentioned above were found both in allografts and autografts, the expressions beinghigher in autografts than allografts; (2) In autografts, TGFα expression began to rise from second post transplantday (D2), reaching a peak on D18,. TGFβ1 expression gained a high level on D2 and had itS peak on D18; PDGFexpression had its peak on D4, rebounding on D25; bFGF expression, exceedingly high on D2, dropped andrebounded in the same manner as in PDGF; (3) In the early stage of the wound healing, mRNA expression of thefour growth factors was lower in allografts, rising up in varying degrees thereafter. Conclusion (1) Graftsurvival took place both in autografts and allografts, with the former pederming better than the latter; (2) Thecombining action of growth factors that reached the affected area through autosecretion and bysecretion probablypromoted the healing of the burn wound, following signal transmission channels; (3) Rejection, which occurred inallogralts, was responsible for the decreasing viability of allografts and their replacement by the host skin.
文摘This study aimed to investigate the potential of a skin care product composed of hyaluronic acid (HA) and collagen (Col) sponge containing epidermal growth factor (EGF), vitamin C derivative (VC), glucosylceramide (GC), poly(γ-glutamic acid) (PGA), and argentine (Arg). High-molecular weight HA aqueous solution, hydrolyzed low-molecular weight HA aqueous solution, and heat- denatured Col aqueous solution were mixed, into which each aqueous solution containing EGF, VC, GC, PGA, or Arg were added, followed by freeze-drying to obtain a spongy EGF-incorporating skin care product (EGF-skin care product). In order to evaluate the first efficacy of EGF, fibroblast proliferation was assessed after 6 days of cultivation in the conditioned medium prepared by dissolving EGF-skin care product in a conventional culture medium. The fibroblast densities increased more effectively in conditioned medium with EGF than in control medium without EGF. In order to evaluate the second efficacy of EGF, the amount of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) produced by fibroblasts were assessed in a wound surface model. A fibroblast-incorporating Col gel sheet (cultured dermal substitute: CDS) was elevated to the air- medium interface, onto which a spongy sheet of EGF-skin care product was placed and cultured for 7 days. The condition covered with or without EGF-skin care product is divided into (+) EGF or (-) EGF, respectively. Fibroblasts in the CDS released 3.7 times more VEGF and 25 times more HGF in (+) EGF compared with (-) EGF. In another experiment, an aqueous solution of EGF-skin care product was added onto CDS and cultured for 7 days. Aqueous solutions were prepared and stored at 4°C or 37°C for a different period of 1 day, 2 weeks, and 4 weeks, respectively. Fibroblasts in CDS under different condition released similar amount of VEGF and HGF. This result indicated that the efficacy of EGF was maintained even after preservation at 37°C for 4 weeks. These findings suggest that EGF-skin care product can be used on damaged skin surface by placing its spongy sheet or its solution.
文摘目的:NOD样受体热蛋白结构域相关蛋白6(NOD-like receptor thermal protein domain associated protein 6,NLRP6)是新发现的寡聚化核苷酸结合结构域样受体家族成员,广泛表达于肠道、肝脏、肾脏、脾脏和肌肉等组织器官,在炎症、焦亡和自噬等多种生物过程发挥广泛的调节作用。近期研究表明,NLRP6在应激条件下对多种组织器官的疾病表型具有重要影响。然而,NLRP6对组织器官生长发育的影响尚未可知。方法:采用CRISPR/Cas9基因编辑技术构建NLRP6基因敲除小鼠,饲养并记录小鼠的生长和繁殖情况。将小鼠的脾、肝、心、肾、脑、四肢和后背皮肤等组织器官进行解剖和切片染色,以评估NLRP6基因敲除对实质器官的宏观发育影响和对组织结构的微观影响。结果:在自然状态下,NLRP6基因敲除缩短了雄鼠的性成熟期并使成年雄鼠的睾丸发生不可逆的破溃与萎缩。在四肢发育上,NLRP6基因敲除诱导成年雄鼠后肢横纹肌断裂,导致后肢明显萎缩。在脾脏发育上,NLRP6基因敲除不仅显著增加了雄鼠的脾脏体积(P<0.01)还诱导雄鼠脾脏出现炎性细胞浸润。在后背皮肤上,NLRP6基因敲除引发雄鼠后背皮肤出现明显的溃疡损伤、胶原纤维增生和炎性细胞浸润。结论:在自然生长发育条件下,NLRP6基因敲除选择性影响小鼠的生殖器发育与性成熟期、后肢肌肉发育、脾脏大小及其炎症免疫状态以及后背皮肤的结构完整性,而且这一作用具有明显的雄激素依赖性。
